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Gene information
General information
TC005424 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13367004..13387692
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005424
    iBeetle screen iB_00856

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005424 RA
      MSSKPGSSK SVESQRPVP PVLRDVGKL AAGVASVST 
      GGSATGNLR NKYALAPGH SLMDWIRLG SSGKDLTGV 
      GSQAGNLSV TPSELALHN KETDAWLCI RGRVYNVTA 
      YLPFHPGGP EQLMKGAGK DATRLFEEV HPWVNFDQI 
      LTKCYVGKL KAHPEFEPE EVFKSEKNA PPPPQNNET 
      VANATKEPI LLPRFDWIQ KLDYITIIF YTGNFSNPM 
      LETTTRGDD NTHSIFLTY NNTIFDNEI VFAGKVHFP 
      GRIKVTCET GKVELCYKK CQGSVWDNF GVLKQKARP 
      YTDSLVQAK LKCKVVNKI KVNYNTVLI ELERSDNTK 
      SVVPLGKHI KVFDTIKGE EIVRSYTPV PICLFSKFR 
      QQNYTTDNI CLMVKRYPE GNLSKILGD CLTGDTVTI 
      SKPLGSFNL QEIEKRETF IILAAGTGI TPMFAIILF 
      LLERRIRKC QRLRLLFFN RTPDDIPFR TQFEELQRE 
      EPRFKVFHV LSQADNTWT GLRGHVSRS ILEETIADH 
      LKDTTYVKS DVYFMVCGP TIFTTLTQQ LFKDLQFKD 
      EQIHFFLG
    CDS sequence
    • >TC005424 RA
      atgagttcg aagcccggc agttcgaaa agtgtggag 
      tctcaaaga ccggtgccc cccgttttg cgcgatgtt 
      ggcaagctg gcagcaggc gtcgcctcc gtcagcacc 
      gggggatcg gcaacagga aacctccgc aacaaatat 
      gcgctcgct cccggccac tccctcatg gactggatc 
      cggttgggc tcctccggc aaggacctc accggcgtg 
      ggttcacag gccggcaac ctttcggtg acacccagc 
      gagctcgcc ctgcataac aaggagacg gacgcctgg 
      ctatgcatc cgagggcgg gtgtacaac gtcaccgca 
      tacctgccg ttccatcct gggggcccc gagcagctc 
      atgaagggg gcgggaaaa gacgccacc cgattgttc 
      gaagaggtg catccctgg gtcaacttc gaccaaatt 
      ctaaccaag tgttacgtt ggtaaattg aaagcacat 
      ccggaattt gagccggaa gaagtgttc aaaagcgag 
      aaaaacgca ccgccacca ccacaaaac aatgagact 
      gtcgcaaat gccacaaag gaaccaatt ttgttaccg 
      agattcgac tggatccaa aagttggat tacattacg 
      attattttt tataccggg aatttttcg aacccgatg 
      cttgagacg acgactcgg ggcgatgat aacacacac 
      agtattttt ttaacttat aataacacg atcttcgat 
      aatgagatt gtctttgcc gggaaggtg cactttccg 
      ggacggatt aaagtcacg tgtgagacg gggaaagtc 
      gagttgtgt tataagaaa tgccaagga agtgtttgg 
      gataatttt ggggtactt aagcagaag gcaaggcct 
      tacactgac tcgcttgtc caagccaaa ttaaaatgt 
      aaagtcgtt aacaagatc aaagttaac tacaacacg 
      gttcttatc gaattggaa cgaagtgat aacaccaaa 
      agtgtcgtc ccgctcggg aaacacatc aaggttttt 
      gacacaatc aaaggggag gaaatcgtc cggagctac 
      acccccgtc cccatctgc cttttcagc aaattccgc 
      caacagaat tacaccact gacaatatt tgtctcatg 
      gtgaaacgc taccccgag ggaaatctt agcaaaata 
      cttggagat tgtctaact ggcgacact gttacaatt 
      tccaagcct ttagggtct ttcaattta caagaaatc 
      gaaaaacgt gagactttc attatccta gctgctggc 
      acaggcatc acgcccatg tttgccata atattgttc 
      ctcctagag cgaagaata agaaaatgt cagcgtttg 
      cgactgctg tttttcaac cgcaccccc gacgacatc 
      cccttccga acccagttc gaggagctc caaagggag 
      gagccccgt tttaaggtg ttccacgtg ttgtcgcag 
      gccgataac acgtggacg gggctcagg gggcacgta 
      agtcggtca atcctcgag gaaacaatc gccgatcat 
      ttgaaagac accacctat gttaaaagc gacgtctat 
      tttatggtg tgtggtcct acgattttc acaactttg 
      acgcaacaa ttgttcaaa gatttgcaa tttaaagac 
      gaacaaatt cactttttc ttaggatag
    mRNA sequence
    • >TC005424 RA
      ttaaaaacc cccaaacgc gtgcgacaa tttgaataa 
      ttaatcaca aaatcgaaa tgttgaccc gtgacaaaa 
      aacccaccc ttgcaaccc ccaataact gaaaaaaca 
      acaagttta caaaaagtg aggttacgt catctgtca 
      aaccggccg attttttgc gaaaaaatc ggccaaaaa 
      cgcgagtgt gaccgttcg agttcatgt ggtgattgc 
      gggctccat gagttcgaa gcccggcag ttcgaaaag 
      tgtggagtc tcaaagacc ggtgccccc cgttttgcg 
      cgatgttgg caagctggc agcaggcgt cgcctccgt 
      cagcaccgg gggatcggc aacaggaaa cctccgcaa 
      caaatatgc gctcgctcc cggccactc cctcatgga 
      ctggatccg gttgggctc ctccggcaa ggacctcac 
      cggcgtggg ttcacaggc cggcaacct ttcggtgac 
      acccagcga gctcgccct gcataacaa ggagacgga 
      cgcctggct atgcatccg agggcgggt gtacaacgt 
      caccgcata cctgccgtt ccatcctgg gggccccga 
      gcagctcat gaagggggc gggaaaaga cgccacccg 
      attgttcga agaggtgca tccctgggt caacttcga 
      ccaaattct aaccaagtg ttacgttgg taaattgaa 
      agcacatcc ggaatttga gccggaaga agtgttcaa 
      aagcgagaa aaacgcacc gccaccacc acaaaacaa 
      tgagactgt cgcaaatgc cacaaagga accaatttt 
      gttaccgag attcgactg gatccaaaa gttggatta 
      cattacgat tatttttta taccgggaa tttttcgaa 
      cccgatgct tgagacgac gactcgggg cgatgataa 
      cacacacag tattttttt aacttataa taacacgat 
      cttcgataa tgagattgt ctttgccgg gaaggtgca 
      ctttccggg acggattaa agtcacgtg tgagacggg 
      gaaagtcga gttgtgtta taagaaatg ccaaggaag 
      tgtttggga taattttgg ggtacttaa gcagaaggc 
      aaggcctta cactgactc gcttgtcca agccaaatt 
      aaaatgtaa agtcgttaa caagatcaa agttaacta 
      caacacggt tcttatcga attggaacg aagtgataa 
      caccaaaag tgtcgtccc gctcgggaa acacatcaa 
      ggtttttga cacaatcaa aggggagga aatcgtccg 
      gagctacac ccccgtccc catctgcct tttcagcaa 
      attccgcca acagaatta caccactga caatatttg 
      tctcatggt gaaacgcta ccccgaggg aaatcttag 
      caaaatact tggagattg tctaactgg cgacactgt 
      tacaatttc caagccttt agggtcttt caatttaca 
      agaaatcga aaaacgtga gactttcat tatcctagc 
      tgctggcac aggcatcac gcccatgtt tgccataat 
      attgttcct cctagagcg aagaataag aaaatgtca 
      gcgtttgcg actgctgtt tttcaaccg cacccccga 
      cgacatccc cttccgaac ccagttcga ggagctcca 
      aagggagga gccccgttt taaggtgtt ccacgtgtt 
      gtcgcaggc cgataacac gtggacggg gctcagggg 
      gcacgtaag tcggtcaat cctcgagga aacaatcgc 
      cgatcattt gaaagacac cacctatgt taaaagcga 
      cgtctattt tatggtgtg tggtcctac gattttcac 
      aactttgac gcaacaatt gttcaaaga tttgcaatt 
      taaagacga acaaattca ctttttctt aggatagca 
      ggccgtcga tcacttcgt cttgagcat ttttcagtt 
      aataggaga gaaaagagc aataattca gtcctagaa 
      cgcacgcgc tacgtgaac acagcctgg gtaggtttg 
      ggcgcgggc aacaaaaaa taaaactgt tttttactt 
      aaaacaaat cgcaaattc ccccaaagg agcggaaaa 
      aatcaaaat tgaggctac gtcggtgac gtcacaaca 
      cttgacagt tatgacacc aattacaaa catggctta 
      tcacttatt tctttgtac ggttttgcg cttgcac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY