Status
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Gene information
General information
TC005421 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13439533..13442001
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005421
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00854

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005421 RA
      MAKIKKKTI TKRPQKAKK AKLNKTKKS EDYSSMSVD 
      KFLDQDFEA SASDEETNH DSVEESSGD EIQEHKAAL 
      SKLKDTDPE FYKFLQDND KKLLEFNLS DEESDDEES 
      APHKPPEEL EVASDESDF EDDSQVKET GTVTLKMLK 
      SWENSIQSD KSNKTITTV MQAFHAALL RVSSEEGQE 
      QSNFKVEGP AVFNGVIQL CVLHLGPAL RRFLGLSDG 
      SKQPPHKCK RFVKVRRVL KDYFMDLSK LLKGVTSAN 
      IQTVLLKHL HYLSPFMVS YPNITKTIL KRLITIWGT 
      AEEGVRVLA FLCILRITN GQKAKFLDT VLKSMYMTY 
      VKNSKFVSV GSLACINFM RRSLVEMFA LDAGVAYQH 
      VFLYIRQLA IYLRNAITV NKKENIHAV YNWQFVNSL 
      KLWGSFLSA SYHKMQLAQ LVYPLVQVC LGVLKLVPT 
      AQYYPLRFH VVKILMDLS RDTGVFIPI LPFILEVLS 
      GYDFNKKHQ KVSMKPIQF TFLLRVSKS QMMENGFKD 
      ALIEAIYEL LLEYLTTNS HSISFPDLS LLCVVQIKQ 
      FVKKCANAN YNRKMKQLL EKIEQNSKF IENERSKTT 
      LNITDFKQI EGWEAQVKT KGTPLATFY DSWIKLHKV 
      KKNKQLTNN EELGEYNLP TIKKKPAKV RNEGPVELF 
      PSDSEDEDG GEEKKRKRG KRGGKNVNK KIKDESGPV 
      EDEGGADIV EDLKMSDW
    CDS sequence
    • >TC005421 RA
      atggcaaaa atcaagaag aaaaccatt accaagcgc 
      ccccaaaag gcgaagaag gcgaaatta aacaaaact 
      aagaaatcg gaagactac agttcaatg tcagtcgat 
      aaattcctc gatcaagat tttgaagca agtgcttct 
      gacgaggaa actaaccac gattcagtc gaggaatcg 
      tcaggagac gaaatacag gaacacaaa gccgccctg 
      agcaaacta aaagacacg gatcccgaa ttttacaaa 
      ttcttgcaa gacaatgac aaaaaattg cttgaattc 
      aacctttcg gacgaagag tctgacgat gaggaaagc 
      gccccacac aaaccacca gaagaattg gaagtcgcg 
      agtgacgaa agtgacttt gaggacgac tcccaagtc 
      aaagaaacg ggcacagtg actttaaaa atgctcaaa 
      agttgggaa aattcaata cagagcgac aaatcaaac 
      aagactata accacagtc atgcaggcg tttcacgcc 
      gccttgctt agagtcagc tcagaggag gggcaagaa 
      caatcgaat ttcaaggtc gagggcccc gctgtcttc 
      aacggcgtg atccaattg tgtgttttg catttgggg 
      cccgccttg cgccgcttt ctggggctg tcggacggc 
      tcaaagcag cccccacac aagtgcaag aggttcgtc 
      aaggtcagg cgcgtcctt aaggactat ttcatggac 
      ttgtctaag ttattgaaa ggcgtcact tctgccaac 
      attcaaacc gtcctcctc aaacacctc cactacctg 
      tccccgttc atggtctcc taccccaac ataactaaa 
      acgatcctg aaacgcctg atcacgatt tggggcacc 
      gccgaggaa ggggtacgt gttttagct ttcttgtgc 
      attttgcgt attaccaac ggccaaaaa gcgaaattt 
      ttggacacg gtcctgaaa tcgatgtat atgacctat 
      gtgaaaaat tcgaaattt gtgagcgtt ggctctttg 
      gcgtgcata aatttcatg cggcggtct ttggttgaa 
      atgtttgcg ttagatgcg ggtgttgct tatcaacac 
      gtttttttg tacatccgc caattggcc atttattta 
      cgtaacgcc atcactgtt aacaaaaaa gaaaacatt 
      catgcggtc tataattgg caatttgtc aattcttta 
      aaactgtgg gggagtttt ctaagtgcg agttatcac 
      aaaatgcag ttggcacag ctggtgtac cctctagtg 
      caagtgtgt ttgggtgtg cttaagttg gtacccact 
      gcccaatat tacccgttg aggtttcac gtggtgaaa 
      attttgatg gatttgtcg cgtgatacg ggcgttttt 
      atcccgatc ctgcctttt atccttgaa gtcctgagc 
      ggttatgat tttaataag aagcaccaa aaagtttcg 
      atgaaaccg atccaattc acatttttg ttgcgcgtc 
      tcaaagtcg caaatgatg gaaaatggg ttcaaggat 
      gcgcttatt gaggcgatt tacgagctg ttgctcgaa 
      tatttaaca acgaattcg cacagtatt agttttcca 
      gatcttagt cttttgtgt gttgttcag atcaaacag 
      tttgttaaa aaatgcgct aatgcgaat tacaaccgg 
      aaaatgaaa caacttctc gagaaaatc gagcaaaat 
      tcgaaattt atcgaaaat gagaggagt aaaactacg 
      ttgaatatc accgatttt aagcaaatt gagggctgg 
      gaggcgcaa gttaagaca aaggggacg cctcttgcc 
      acgttttat gacagttgg attaagctt cataaagtt 
      aagaagaac aaacagttg actaataat gaagagttg 
      ggagagtat aatttgccg acgattaag aaaaagccg 
      gcgaaagtt cgaaatgaa ggtccggtt gaattgttc 
      ccgagtgat agtgaagat gaggatggg ggggaagaa 
      aagaaacgg aaaagggga aagagaggt gggaagaat 
      gttaataag aagattaag gacgaaagt gggcctgtt 
      gaagatgaa ggaggggct gatattgtt gaggatttg 
      aaaatgagc gattggtga
    mRNA sequence
    • >TC005421 RA
      aacactgct ttgcccttt ttgaatgag aattaatcg 
      ataaatcac gtccttcaa ttcatgata gacgaaaaa 
      tcagcaaaa aatcgttaa ttattcttc gttgctaac 
      ttcactcat gtaactcat aaccgcgaa gtgtcataa 
      cctctcagt ttttcacgt gttgtttaa aatggcaaa 
      aatcaagaa gaaaaccat taccaagcg cccccaaaa 
      ggcgaagaa ggcgaaatt aaacaaaac taagaaatc 
      ggaagacta cagttcaat gtcagtcga taaattcct 
      cgatcaaga ttttgaagc aagtgcttc tgacgagga 
      aactaacca cgattcagt cgaggaatc gtcaggaga 
      cgaaataca ggaacacaa agccgccct gagcaaact 
      aaaagacac ggatcccga attttacaa attcttgca 
      agacaatga caaaaaatt gcttgaatt caacctttc 
      ggacgaaga gtctgacga tgaggaaag cgccccaca 
      caaaccacc agaagaatt ggaagtcgc gagtgacga 
      aagtgactt tgaggacga ctcccaagt caaagaaac 
      gggcacagt gactttaaa aatgctcaa aagttggga 
      aaattcaat acagagcga caaatcaaa caagactat 
      aaccacagt catgcaggc gtttcacgc cgccttgct 
      tagagtcag ctcagagga ggggcaaga acaatcgaa 
      tttcaaggt cgagggccc cgctgtctt caacggcgt 
      gatccaatt gtgtgtttt gcatttggg gcccgcctt 
      gcgccgctt tctggggct gtcggacgg ctcaaagca 
      gcccccaca caagtgcaa gaggttcgt caaggtcag 
      gcgcgtcct taaggacta tttcatgga cttgtctaa 
      gttattgaa aggcgtcac ttctgccaa cattcaaac 
      cgtcctcct caaacacct ccactacct gtccccgtt 
      catggtctc ctaccccaa cataactaa aacgatcct 
      gaaacgcct gatcacgat ttggggcac cgccgagga 
      aggggtacg tgttttagc tttcttgtg cattttgcg 
      tattaccaa cggccaaaa agcgaaatt tttggacac 
      ggtcctgaa atcgatgta tatgaccta tgtgaaaaa 
      ttcgaaatt tgtgagcgt tggctcttt ggcgtgcat 
      aaatttcat gcggcggtc tttggttga aatgtttgc 
      gttagatgc gggtgttgc ttatcaaca cgttttttt 
      gtacatccg ccaattggc catttattt acgtaacgc 
      catcactgt taacaaaaa agaaaacat tcatgcggt 
      ctataattg gcaatttgt caattcttt aaaactgtg 
      ggggagttt tctaagtgc gagttatca caaaatgca 
      gttggcaca gctggtgta ccctctagt gcaagtgtg 
      tttgggtgt gcttaagtt ggtacccac tgcccaata 
      ttacccgtt gaggtttca cgtggtgaa aattttgat 
      ggatttgtc gcgtgatac gggcgtttt tatcccgat 
      cctgccttt tatccttga agtcctgag cggttatga 
      ttttaataa gaagcacca aaaagtttc gatgaaacc 
      gatccaatt cacattttt gttgcgcgt ctcaaagtc 
      gcaaatgat ggaaaatgg gttcaagga tgcgcttat 
      tgaggcgat ttacgagct gttgctcga atatttaac 
      aacgaattc gcacagtat tagttttcc agatcttag 
      tcttttgtg tgttgttca gatcaaaca gtttgttaa 
      aaaatgcgc taatgcgaa ttacaaccg gaaaatgaa 
      acaacttct cgagaaaat cgagcaaaa ttcgaaatt 
      tatcgaaaa tgagaggag taaaactac gttgaatat 
      caccgattt taagcaaat tgagggctg ggaggcgca 
      agttaagac aaaggggac gcctcttgc cacgtttta 
      tgacagttg gattaagct tcataaagt taagaagaa 
      caaacagtt gactaataa tgaagagtt gggagagta 
      taatttgcc gacgattaa gaaaaagcc ggcgaaagt 
      tcgaaatga aggtccggt tgaattgtt cccgagtga 
      tagtgaaga tgaggatgg gggggaaga aaagaaacg 
      gaaaagggg aaagagagg tgggaagaa tgttaataa 
      gaagattaa ggacgaaag tgggcctgt tgaagatga 
      aggaggggc tgatattgt tgaggattt gaaaatgag 
      cgattggtg attgtttca gtaaaaatt atttttatt 
      atgttattt taataaagt gattttgaa ata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY