| General information | |||
| TC005420 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 13452723..13455221 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC005420 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00853 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC005420 RA
MWQKYDENL HNNPFFHEI IKNHGDLIV KASQEEWII CVPRIGVID PNEITIETI LDHILVPSE GTTLSKKEA TIKNKQIVT NSTTRHILF EETFYIEDL KYTVFCIDS PLFSSRVTC PHVVNKIET LHHCIDFFW SENLSHVLI KIQRLCASF VDQNRSFCR ENLQNQKDL IGSLYSQCL QLVLRNRGF TDCDNIKLA VETYMQSCL DQFLTHAIN TVTCLHDSE LNKIIRNSS ELQLKDLSI PPKFCDNIT NAKSELSKI NNYVTILDK VNCLKLTFG HLYTKICLT SDDVLQLFV FLILKLNIT NWTSNLIYL TQFQFSSHE PNESNFLVT SLEAALEFI KSEQFAKIK PRHSVTPLF QTIVTGDLT NLRKLLDVK NSGGNLCHP LCDCDDCTK MDSSYDINS KGQTLLTFA ALLGRSEIV EFLLSKGAE VNSVDYFGR SALHYACLK GHQDVLLIL VNCRAEVNV RDHDGNTPL HVACNNGHE NCVKALFYA SKGLDIDCV NKHGDSPLH LAVKWNYVG ICAVLIENN ANVCVTNRK NQLPANLAQ NYYTERFFP NSVPKPPKD NNRSDKFKK IDLLLKAIE NDDLPLTCF YLGFSTSSH YDFQSKCHP LCNCEKCED LDTGQDPNV EYISVNACN IDGFTPLHF AAKFGRTEL LRILLDSGA LPNLKTLKT LQTPLHLAC IHQQVSVVK ELVKCANCE VDSQDYYGN TPLYYACVR NDAKIVEVL LTNGANADI RDYGNSIIK QVQENLTES QDIFL
- >TC005420 RA
atgtggcaa aaatacgac gaaaacctc cacaacaat ccttttttc cacgaaatt atcaaaaat cacggtgac ttaatagtg aaagcgtca caagaagag tggattatt tgtgttcca agaatcggt gtcattgac cccaatgaa ataacaatt gagacgatt cttgaccat attctcgtg ccctcagaa ggcacaaca ttgagcaaa aaggaagcc acaatcaaa aacaaacaa atcgtaact aattcaaca acccgtcac atacttttc gaggaaacg ttctacata gaagacttg aaatataca gttttctgt attgatagc cccctgttc tcaagccga gtgacatgc ccccatgta gtcaacaaa attgagaca ctccaccac tgtattgat tttttctgg agtgagaat ttgagccac gttttaatc aaaatccag cgactgtgt gcaagtttc gtcgaccag aatcgtagt ttttgccgc gaaaatttg caaaaccag aaggattta atcgggagt ttgtactcc cagtgtttg caacttgtg ttgcgtaat cggggattc accgactgt gacaacatc aagctagcg gtggaaaca tacatgcag agctgtctt gaccagttc ctcacccat gctataaac acagtgacg tgcctccat gactccgaa ttgaacaaa atcatccga aactcaagc gagcttcaa ttgaaagac ttgtccatc cctccgaaa ttctgtgat aatattacg aacgctaaa agcgaacta agcaaaata aataactac gttacgatc ctagacaag gttaattgc ctaaaatta acgttcggt catttatac accaagatt tgtttaacc tccgacgat gttttacaa cttttcgtc ttcctaatt cttaaacta aacatcacc aactggact tcaaaccta atttactta acccaattc cagttcagt tcgcacgaa cccaacgaa agtaatttc ctcgtcact tcgctggaa gcggcttta gaattcatc aagagcgag caattcgcc aaaatcaaa cctcgccat tcggtgact cccctattc caaacaatc gtcaccggc gacttgacc aacttgcgt aaactgcta gacgtgaag aacagtggc ggtaatttg tgtcatccg ttgtgcgat tgcgacgat tgcacaaaa atggactcg agttatgat ataaacagc aaaggtcag actcttctc acttttgcc gccttgttg gggcgcagt gaaatcgtc gaatttttg ctaagtaaa ggggctgag gtcaactct gttgattat ttcgggcga agtgcgttg cattacgcc tgccttaaa ggccaccaa gacgtttta ttaattcta gtcaactgt cgggctgag gtcaatgtg agagaccat gacgggaat actccctta catgtcgct tgtaacaat gggcatgag aattgcgtc aaagctttg ttctacgcc tccaaaggc ttggacatc gattgtgtc aataagcac ggggacagt ccgctgcat ttggccgtc aaatggaat tatgtgggg atatgtgcg gtcttgatc gagaataac gccaatgtt tgtgttact aatcgcaag aatcaacta ccagcaaac ttggcacag aactactac accgagcga tttttcccc aactctgtc ccaaaaccc ccaaaggat aataacaga agcgacaaa ttcaaaaaa atcgatctg ttactcaaa gccatcgaa aacgatgat ttacccttg acttgtttt tatctgggt ttttcgacc agttcccac tatgatttt caatcaaag tgtcaccct ttatgcaac tgcgaaaag tgtgaagat ttagacact gggcaggac cccaatgtt gaatatatc agcgttaat gcgtgcaat attgacggt tttacgcca cttcatttc gccgcgaaa tttggaagg acggaacta ctccgtatc ctactagat tccggtgct ttacccaac ttgaaaact ttaaaaaca ctacaaact ccactacac ttggcttgc atccaccaa caagtttcc gtggtgaaa gaattggtc aaatgtgca aattgtgaa gtggattcg caagattat tacggaaac acgcctctc tattatgca tgcgttcga aatgacgcc aaaattgtc gaagtattg ctcacaaac ggagccaat gccgatatt agggattat ggaaactcg ataataaaa caagtccaa gaaaatctc accgagagc caggatatt ttcttgtga
- >TC005420 RA
gactttagg aggccaata actcaataa ctatatttt ctgacccat tctttcaaa gccactcgg tattttgaa ctgcattaa agtaaattt ctactttaa ttttggcca attcactaa aatttgaaa atggcgcac ttgacacgc atgtgtgcc gtcacctta gccacacac atgtcaaaa aaataacct caaaatgtg gcaaaaata cgacgaaaa cctccacaa caatccttt tttccacga aattatcaa aaatcacgg tgacttaat agtgaaagc gtcacaaga agagtggat tatttgtgt tccaagaat cggtgtcat tgaccccaa tgaaataac aattgagac gattcttga ccatattct cgtgccctc agaaggcac aacattgag caaaaagga agccacaat caaaaacaa acaaatcgt aactaattc aacaacccg tcacatact tttcgagga aacgttcta catagaaga cttgaaata tacagtttt ctgtattga tagccccct gttctcaag ccgagtgac atgccccca tgtagtcaa caaaattga gacactcca ccactgtat tgatttttt ctggagtga gaatttgag ccacgtttt aatcaaaat ccagcgact gtgtgcaag tttcgtcga ccagaatcg tagtttttg ccgcgaaaa tttgcaaaa ccagaagga tttaatcgg gagtttgta ctcccagtg tttgcaact tgtgttgcg taatcgggg attcaccga ctgtgacaa catcaagct agcggtgga aacatacat gcagagctg tcttgacca gttcctcac ccatgctat aaacacagt gacgtgcct ccatgactc cgaattgaa caaaatcat ccgaaactc aagcgagct tcaattgaa agacttgtc catccctcc gaaattctg tgataatat tacgaacgc taaaagcga actaagcaa aataaataa ctacgttac gatcctaga caaggttaa ttgcctaaa attaacgtt cggtcattt atacaccaa gatttgttt aacctccga cgatgtttt acaactttt cgtcttcct aattcttaa actaaacat caccaactg gacttcaaa cctaattta cttaaccca attccagtt cagttcgca cgaacccaa cgaaagtaa tttcctcgt cacttcgct ggaagcggc tttagaatt catcaagag cgagcaatt cgccaaaat caaacctcg ccattcggt gactcccct attccaaac aatcgtcac cggcgactt gaccaactt gcgtaaact gctagacgt gaagaacag tggcggtaa tttgtgtca tccgttgtg cgattgcga cgattgcac aaaaatgga ctcgagtta tgatataaa cagcaaagg tcagactct tctcacttt tgccgcctt gttggggcg cagtgaaat cgtcgaatt tttgctaag taaaggggc tgaggtcaa ctctgttga ttatttcgg gcgaagtgc gttgcatta cgcctgcct taaaggcca ccaagacgt tttattaat tctagtcaa ctgtcgggc tgaggtcaa tgtgagaga ccatgacgg gaatactcc cttacatgt cgcttgtaa caatgggca tgagaattg cgtcaaagc tttgttcta cgcctccaa aggcttgga catcgattg tgtcaataa gcacgggga cagtccgct gcatttggc cgtcaaatg gaattatgt ggggatatg tgcggtctt gatcgagaa taacgccaa tgtttgtgt tactaatcg caagaatca actaccagc aaacttggc acagaacta ctacaccga gcgattttt ccccaactc tgtcccaaa acccccaaa ggataataa cagaagcga caaattcaa aaaaatcga tctgttact caaagccat cgaaaacga tgatttacc cttgacttg tttttatct gggtttttc gaccagttc ccactatga ttttcaatc aaagtgtca ccctttatg caactgcga aaagtgtga agatttaga cactgggca ggaccccaa tgttgaata tatcagcgt taatgcgtg caatattga cggttttac gccacttca tttcgccgc gaaatttgg aaggacgga actactccg tatcctact agattccgg tgctttacc caacttgaa aactttaaa aacactaca aactccact acacttggc ttgcatcca ccaacaagt ttccgtggt gaaagaatt ggtcaaatg tgcaaattg tgaagtgga ttcgcaaga ttattacgg aaacacgcc tctctatta tgcatgcgt tcgaaatga cgccaaaat tgtcgaagt attgctcac aaacggagc caatgccga tattaggga ttatggaaa ctcgataat aaaacaagt ccaagaaaa tctcaccga gagccagga tattttctt gtgaaataa aattttata cacttt
