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Gene information
General information
TC005420 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13452723..13455221
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC005420
    iBeetle screen iB_00853

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005420 RA
      MWQKYDENL HNNPFFHEI IKNHGDLIV KASQEEWII 
CVPRIGVID PNEITIETI LDHILVPSE GTTLSKKEA 
TIKNKQIVT NSTTRHILF EETFYIEDL KYTVFCIDS 
PLFSSRVTC PHVVNKIET LHHCIDFFW SENLSHVLI 
KIQRLCASF VDQNRSFCR ENLQNQKDL IGSLYSQCL 
QLVLRNRGF TDCDNIKLA VETYMQSCL DQFLTHAIN 
TVTCLHDSE LNKIIRNSS ELQLKDLSI PPKFCDNIT 
NAKSELSKI NNYVTILDK VNCLKLTFG HLYTKICLT 
SDDVLQLFV FLILKLNIT NWTSNLIYL TQFQFSSHE 
PNESNFLVT SLEAALEFI KSEQFAKIK PRHSVTPLF 
QTIVTGDLT NLRKLLDVK NSGGNLCHP LCDCDDCTK 
MDSSYDINS KGQTLLTFA ALLGRSEIV EFLLSKGAE 
VNSVDYFGR SALHYACLK GHQDVLLIL VNCRAEVNV 
RDHDGNTPL HVACNNGHE NCVKALFYA SKGLDIDCV 
NKHGDSPLH LAVKWNYVG ICAVLIENN ANVCVTNRK 
NQLPANLAQ NYYTERFFP NSVPKPPKD NNRSDKFKK 
IDLLLKAIE NDDLPLTCF YLGFSTSSH YDFQSKCHP 
LCNCEKCED LDTGQDPNV EYISVNACN IDGFTPLHF 
AAKFGRTEL LRILLDSGA LPNLKTLKT LQTPLHLAC 
IHQQVSVVK ELVKCANCE VDSQDYYGN TPLYYACVR 
NDAKIVEVL LTNGANADI RDYGNSIIK QVQENLTES 
QDIFL
    CDS sequence
    • >TC005420 RA
      atgtggcaa aaatacgac gaaaacctc cacaacaat 
ccttttttc cacgaaatt atcaaaaat cacggtgac 
ttaatagtg aaagcgtca caagaagag tggattatt 
tgtgttcca agaatcggt gtcattgac cccaatgaa 
ataacaatt gagacgatt cttgaccat attctcgtg 
ccctcagaa ggcacaaca ttgagcaaa aaggaagcc 
acaatcaaa aacaaacaa atcgtaact aattcaaca 
acccgtcac atacttttc gaggaaacg ttctacata 
gaagacttg aaatataca gttttctgt attgatagc 
cccctgttc tcaagccga gtgacatgc ccccatgta 
gtcaacaaa attgagaca ctccaccac tgtattgat 
tttttctgg agtgagaat ttgagccac gttttaatc 
aaaatccag cgactgtgt gcaagtttc gtcgaccag 
aatcgtagt ttttgccgc gaaaatttg caaaaccag 
aaggattta atcgggagt ttgtactcc cagtgtttg 
caacttgtg ttgcgtaat cggggattc accgactgt 
gacaacatc aagctagcg gtggaaaca tacatgcag 
agctgtctt gaccagttc ctcacccat gctataaac 
acagtgacg tgcctccat gactccgaa ttgaacaaa 
atcatccga aactcaagc gagcttcaa ttgaaagac 
ttgtccatc cctccgaaa ttctgtgat aatattacg 
aacgctaaa agcgaacta agcaaaata aataactac 
gttacgatc ctagacaag gttaattgc ctaaaatta 
acgttcggt catttatac accaagatt tgtttaacc 
tccgacgat gttttacaa cttttcgtc ttcctaatt 
cttaaacta aacatcacc aactggact tcaaaccta 
atttactta acccaattc cagttcagt tcgcacgaa 
cccaacgaa agtaatttc ctcgtcact tcgctggaa 
gcggcttta gaattcatc aagagcgag caattcgcc 
aaaatcaaa cctcgccat tcggtgact cccctattc 
caaacaatc gtcaccggc gacttgacc aacttgcgt 
aaactgcta gacgtgaag aacagtggc ggtaatttg 
tgtcatccg ttgtgcgat tgcgacgat tgcacaaaa 
atggactcg agttatgat ataaacagc aaaggtcag 
actcttctc acttttgcc gccttgttg gggcgcagt 
gaaatcgtc gaatttttg ctaagtaaa ggggctgag 
gtcaactct gttgattat ttcgggcga agtgcgttg 
cattacgcc tgccttaaa ggccaccaa gacgtttta 
ttaattcta gtcaactgt cgggctgag gtcaatgtg 
agagaccat gacgggaat actccctta catgtcgct 
tgtaacaat gggcatgag aattgcgtc aaagctttg 
ttctacgcc tccaaaggc ttggacatc gattgtgtc 
aataagcac ggggacagt ccgctgcat ttggccgtc 
aaatggaat tatgtgggg atatgtgcg gtcttgatc 
gagaataac gccaatgtt tgtgttact aatcgcaag 
aatcaacta ccagcaaac ttggcacag aactactac 
accgagcga tttttcccc aactctgtc ccaaaaccc 
ccaaaggat aataacaga agcgacaaa ttcaaaaaa 
atcgatctg ttactcaaa gccatcgaa aacgatgat 
ttacccttg acttgtttt tatctgggt ttttcgacc 
agttcccac tatgatttt caatcaaag tgtcaccct 
ttatgcaac tgcgaaaag tgtgaagat ttagacact 
gggcaggac cccaatgtt gaatatatc agcgttaat 
gcgtgcaat attgacggt tttacgcca cttcatttc 
gccgcgaaa tttggaagg acggaacta ctccgtatc 
ctactagat tccggtgct ttacccaac ttgaaaact 
ttaaaaaca ctacaaact ccactacac ttggcttgc 
atccaccaa caagtttcc gtggtgaaa gaattggtc 
aaatgtgca aattgtgaa gtggattcg caagattat 
tacggaaac acgcctctc tattatgca tgcgttcga 
aatgacgcc aaaattgtc gaagtattg ctcacaaac 
ggagccaat gccgatatt agggattat ggaaactcg 
ataataaaa caagtccaa gaaaatctc accgagagc 
caggatatt ttcttgtga
    mRNA sequence
    • >TC005420 RA
      gactttagg aggccaata actcaataa ctatatttt 
ctgacccat tctttcaaa gccactcgg tattttgaa 
ctgcattaa agtaaattt ctactttaa ttttggcca 
attcactaa aatttgaaa atggcgcac ttgacacgc 
atgtgtgcc gtcacctta gccacacac atgtcaaaa 
aaataacct caaaatgtg gcaaaaata cgacgaaaa 
cctccacaa caatccttt tttccacga aattatcaa 
aaatcacgg tgacttaat agtgaaagc gtcacaaga 
agagtggat tatttgtgt tccaagaat cggtgtcat 
tgaccccaa tgaaataac aattgagac gattcttga 
ccatattct cgtgccctc agaaggcac aacattgag 
caaaaagga agccacaat caaaaacaa acaaatcgt 
aactaattc aacaacccg tcacatact tttcgagga 
aacgttcta catagaaga cttgaaata tacagtttt 
ctgtattga tagccccct gttctcaag ccgagtgac 
atgccccca tgtagtcaa caaaattga gacactcca 
ccactgtat tgatttttt ctggagtga gaatttgag 
ccacgtttt aatcaaaat ccagcgact gtgtgcaag 
tttcgtcga ccagaatcg tagtttttg ccgcgaaaa 
tttgcaaaa ccagaagga tttaatcgg gagtttgta 
ctcccagtg tttgcaact tgtgttgcg taatcgggg 
attcaccga ctgtgacaa catcaagct agcggtgga 
aacatacat gcagagctg tcttgacca gttcctcac 
ccatgctat aaacacagt gacgtgcct ccatgactc 
cgaattgaa caaaatcat ccgaaactc aagcgagct 
tcaattgaa agacttgtc catccctcc gaaattctg 
tgataatat tacgaacgc taaaagcga actaagcaa 
aataaataa ctacgttac gatcctaga caaggttaa 
ttgcctaaa attaacgtt cggtcattt atacaccaa 
gatttgttt aacctccga cgatgtttt acaactttt 
cgtcttcct aattcttaa actaaacat caccaactg 
gacttcaaa cctaattta cttaaccca attccagtt 
cagttcgca cgaacccaa cgaaagtaa tttcctcgt 
cacttcgct ggaagcggc tttagaatt catcaagag 
cgagcaatt cgccaaaat caaacctcg ccattcggt 
gactcccct attccaaac aatcgtcac cggcgactt 
gaccaactt gcgtaaact gctagacgt gaagaacag 
tggcggtaa tttgtgtca tccgttgtg cgattgcga 
cgattgcac aaaaatgga ctcgagtta tgatataaa 
cagcaaagg tcagactct tctcacttt tgccgcctt 
gttggggcg cagtgaaat cgtcgaatt tttgctaag 
taaaggggc tgaggtcaa ctctgttga ttatttcgg 
gcgaagtgc gttgcatta cgcctgcct taaaggcca 
ccaagacgt tttattaat tctagtcaa ctgtcgggc 
tgaggtcaa tgtgagaga ccatgacgg gaatactcc 
cttacatgt cgcttgtaa caatgggca tgagaattg 
cgtcaaagc tttgttcta cgcctccaa aggcttgga 
catcgattg tgtcaataa gcacgggga cagtccgct 
gcatttggc cgtcaaatg gaattatgt ggggatatg 
tgcggtctt gatcgagaa taacgccaa tgtttgtgt 
tactaatcg caagaatca actaccagc aaacttggc 
acagaacta ctacaccga gcgattttt ccccaactc 
tgtcccaaa acccccaaa ggataataa cagaagcga 
caaattcaa aaaaatcga tctgttact caaagccat 
cgaaaacga tgatttacc cttgacttg tttttatct 
gggtttttc gaccagttc ccactatga ttttcaatc 
aaagtgtca ccctttatg caactgcga aaagtgtga 
agatttaga cactgggca ggaccccaa tgttgaata 
tatcagcgt taatgcgtg caatattga cggttttac 
gccacttca tttcgccgc gaaatttgg aaggacgga 
actactccg tatcctact agattccgg tgctttacc 
caacttgaa aactttaaa aacactaca aactccact 
acacttggc ttgcatcca ccaacaagt ttccgtggt 
gaaagaatt ggtcaaatg tgcaaattg tgaagtgga 
ttcgcaaga ttattacgg aaacacgcc tctctatta 
tgcatgcgt tcgaaatga cgccaaaat tgtcgaagt 
attgctcac aaacggagc caatgccga tattaggga 
ttatggaaa ctcgataat aaaacaagt ccaagaaaa 
tctcaccga gagccagga tattttctt gtgaaataa 
aattttata cacttt
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