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Gene information
General information
TC005420 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13452723..13455221
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005420
    iBeetle screen iB_00853

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005420 RA
      MWQKYDENL HNNPFFHEI IKNHGDLIV KASQEEWII 
      CVPRIGVID PNEITIETI LDHILVPSE GTTLSKKEA 
      TIKNKQIVT NSTTRHILF EETFYIEDL KYTVFCIDS 
      PLFSSRVTC PHVVNKIET LHHCIDFFW SENLSHVLI 
      KIQRLCASF VDQNRSFCR ENLQNQKDL IGSLYSQCL 
      QLVLRNRGF TDCDNIKLA VETYMQSCL DQFLTHAIN 
      TVTCLHDSE LNKIIRNSS ELQLKDLSI PPKFCDNIT 
      NAKSELSKI NNYVTILDK VNCLKLTFG HLYTKICLT 
      SDDVLQLFV FLILKLNIT NWTSNLIYL TQFQFSSHE 
      PNESNFLVT SLEAALEFI KSEQFAKIK PRHSVTPLF 
      QTIVTGDLT NLRKLLDVK NSGGNLCHP LCDCDDCTK 
      MDSSYDINS KGQTLLTFA ALLGRSEIV EFLLSKGAE 
      VNSVDYFGR SALHYACLK GHQDVLLIL VNCRAEVNV 
      RDHDGNTPL HVACNNGHE NCVKALFYA SKGLDIDCV 
      NKHGDSPLH LAVKWNYVG ICAVLIENN ANVCVTNRK 
      NQLPANLAQ NYYTERFFP NSVPKPPKD NNRSDKFKK 
      IDLLLKAIE NDDLPLTCF YLGFSTSSH YDFQSKCHP 
      LCNCEKCED LDTGQDPNV EYISVNACN IDGFTPLHF 
      AAKFGRTEL LRILLDSGA LPNLKTLKT LQTPLHLAC 
      IHQQVSVVK ELVKCANCE VDSQDYYGN TPLYYACVR 
      NDAKIVEVL LTNGANADI RDYGNSIIK QVQENLTES 
      QDIFL
    CDS sequence
    • >TC005420 RA
      atgtggcaa aaatacgac gaaaacctc cacaacaat 
      ccttttttc cacgaaatt atcaaaaat cacggtgac 
      ttaatagtg aaagcgtca caagaagag tggattatt 
      tgtgttcca agaatcggt gtcattgac cccaatgaa 
      ataacaatt gagacgatt cttgaccat attctcgtg 
      ccctcagaa ggcacaaca ttgagcaaa aaggaagcc 
      acaatcaaa aacaaacaa atcgtaact aattcaaca 
      acccgtcac atacttttc gaggaaacg ttctacata 
      gaagacttg aaatataca gttttctgt attgatagc 
      cccctgttc tcaagccga gtgacatgc ccccatgta 
      gtcaacaaa attgagaca ctccaccac tgtattgat 
      tttttctgg agtgagaat ttgagccac gttttaatc 
      aaaatccag cgactgtgt gcaagtttc gtcgaccag 
      aatcgtagt ttttgccgc gaaaatttg caaaaccag 
      aaggattta atcgggagt ttgtactcc cagtgtttg 
      caacttgtg ttgcgtaat cggggattc accgactgt 
      gacaacatc aagctagcg gtggaaaca tacatgcag 
      agctgtctt gaccagttc ctcacccat gctataaac 
      acagtgacg tgcctccat gactccgaa ttgaacaaa 
      atcatccga aactcaagc gagcttcaa ttgaaagac 
      ttgtccatc cctccgaaa ttctgtgat aatattacg 
      aacgctaaa agcgaacta agcaaaata aataactac 
      gttacgatc ctagacaag gttaattgc ctaaaatta 
      acgttcggt catttatac accaagatt tgtttaacc 
      tccgacgat gttttacaa cttttcgtc ttcctaatt 
      cttaaacta aacatcacc aactggact tcaaaccta 
      atttactta acccaattc cagttcagt tcgcacgaa 
      cccaacgaa agtaatttc ctcgtcact tcgctggaa 
      gcggcttta gaattcatc aagagcgag caattcgcc 
      aaaatcaaa cctcgccat tcggtgact cccctattc 
      caaacaatc gtcaccggc gacttgacc aacttgcgt 
      aaactgcta gacgtgaag aacagtggc ggtaatttg 
      tgtcatccg ttgtgcgat tgcgacgat tgcacaaaa 
      atggactcg agttatgat ataaacagc aaaggtcag 
      actcttctc acttttgcc gccttgttg gggcgcagt 
      gaaatcgtc gaatttttg ctaagtaaa ggggctgag 
      gtcaactct gttgattat ttcgggcga agtgcgttg 
      cattacgcc tgccttaaa ggccaccaa gacgtttta 
      ttaattcta gtcaactgt cgggctgag gtcaatgtg 
      agagaccat gacgggaat actccctta catgtcgct 
      tgtaacaat gggcatgag aattgcgtc aaagctttg 
      ttctacgcc tccaaaggc ttggacatc gattgtgtc 
      aataagcac ggggacagt ccgctgcat ttggccgtc 
      aaatggaat tatgtgggg atatgtgcg gtcttgatc 
      gagaataac gccaatgtt tgtgttact aatcgcaag 
      aatcaacta ccagcaaac ttggcacag aactactac 
      accgagcga tttttcccc aactctgtc ccaaaaccc 
      ccaaaggat aataacaga agcgacaaa ttcaaaaaa 
      atcgatctg ttactcaaa gccatcgaa aacgatgat 
      ttacccttg acttgtttt tatctgggt ttttcgacc 
      agttcccac tatgatttt caatcaaag tgtcaccct 
      ttatgcaac tgcgaaaag tgtgaagat ttagacact 
      gggcaggac cccaatgtt gaatatatc agcgttaat 
      gcgtgcaat attgacggt tttacgcca cttcatttc 
      gccgcgaaa tttggaagg acggaacta ctccgtatc 
      ctactagat tccggtgct ttacccaac ttgaaaact 
      ttaaaaaca ctacaaact ccactacac ttggcttgc 
      atccaccaa caagtttcc gtggtgaaa gaattggtc 
      aaatgtgca aattgtgaa gtggattcg caagattat 
      tacggaaac acgcctctc tattatgca tgcgttcga 
      aatgacgcc aaaattgtc gaagtattg ctcacaaac 
      ggagccaat gccgatatt agggattat ggaaactcg 
      ataataaaa caagtccaa gaaaatctc accgagagc 
      caggatatt ttcttgtga
    mRNA sequence
    • >TC005420 RA
      gactttagg aggccaata actcaataa ctatatttt 
      ctgacccat tctttcaaa gccactcgg tattttgaa 
      ctgcattaa agtaaattt ctactttaa ttttggcca 
      attcactaa aatttgaaa atggcgcac ttgacacgc 
      atgtgtgcc gtcacctta gccacacac atgtcaaaa 
      aaataacct caaaatgtg gcaaaaata cgacgaaaa 
      cctccacaa caatccttt tttccacga aattatcaa 
      aaatcacgg tgacttaat agtgaaagc gtcacaaga 
      agagtggat tatttgtgt tccaagaat cggtgtcat 
      tgaccccaa tgaaataac aattgagac gattcttga 
      ccatattct cgtgccctc agaaggcac aacattgag 
      caaaaagga agccacaat caaaaacaa acaaatcgt 
      aactaattc aacaacccg tcacatact tttcgagga 
      aacgttcta catagaaga cttgaaata tacagtttt 
      ctgtattga tagccccct gttctcaag ccgagtgac 
      atgccccca tgtagtcaa caaaattga gacactcca 
      ccactgtat tgatttttt ctggagtga gaatttgag 
      ccacgtttt aatcaaaat ccagcgact gtgtgcaag 
      tttcgtcga ccagaatcg tagtttttg ccgcgaaaa 
      tttgcaaaa ccagaagga tttaatcgg gagtttgta 
      ctcccagtg tttgcaact tgtgttgcg taatcgggg 
      attcaccga ctgtgacaa catcaagct agcggtgga 
      aacatacat gcagagctg tcttgacca gttcctcac 
      ccatgctat aaacacagt gacgtgcct ccatgactc 
      cgaattgaa caaaatcat ccgaaactc aagcgagct 
      tcaattgaa agacttgtc catccctcc gaaattctg 
      tgataatat tacgaacgc taaaagcga actaagcaa 
      aataaataa ctacgttac gatcctaga caaggttaa 
      ttgcctaaa attaacgtt cggtcattt atacaccaa 
      gatttgttt aacctccga cgatgtttt acaactttt 
      cgtcttcct aattcttaa actaaacat caccaactg 
      gacttcaaa cctaattta cttaaccca attccagtt 
      cagttcgca cgaacccaa cgaaagtaa tttcctcgt 
      cacttcgct ggaagcggc tttagaatt catcaagag 
      cgagcaatt cgccaaaat caaacctcg ccattcggt 
      gactcccct attccaaac aatcgtcac cggcgactt 
      gaccaactt gcgtaaact gctagacgt gaagaacag 
      tggcggtaa tttgtgtca tccgttgtg cgattgcga 
      cgattgcac aaaaatgga ctcgagtta tgatataaa 
      cagcaaagg tcagactct tctcacttt tgccgcctt 
      gttggggcg cagtgaaat cgtcgaatt tttgctaag 
      taaaggggc tgaggtcaa ctctgttga ttatttcgg 
      gcgaagtgc gttgcatta cgcctgcct taaaggcca 
      ccaagacgt tttattaat tctagtcaa ctgtcgggc 
      tgaggtcaa tgtgagaga ccatgacgg gaatactcc 
      cttacatgt cgcttgtaa caatgggca tgagaattg 
      cgtcaaagc tttgttcta cgcctccaa aggcttgga 
      catcgattg tgtcaataa gcacgggga cagtccgct 
      gcatttggc cgtcaaatg gaattatgt ggggatatg 
      tgcggtctt gatcgagaa taacgccaa tgtttgtgt 
      tactaatcg caagaatca actaccagc aaacttggc 
      acagaacta ctacaccga gcgattttt ccccaactc 
      tgtcccaaa acccccaaa ggataataa cagaagcga 
      caaattcaa aaaaatcga tctgttact caaagccat 
      cgaaaacga tgatttacc cttgacttg tttttatct 
      gggtttttc gaccagttc ccactatga ttttcaatc 
      aaagtgtca ccctttatg caactgcga aaagtgtga 
      agatttaga cactgggca ggaccccaa tgttgaata 
      tatcagcgt taatgcgtg caatattga cggttttac 
      gccacttca tttcgccgc gaaatttgg aaggacgga 
      actactccg tatcctact agattccgg tgctttacc 
      caacttgaa aactttaaa aacactaca aactccact 
      acacttggc ttgcatcca ccaacaagt ttccgtggt 
      gaaagaatt ggtcaaatg tgcaaattg tgaagtgga 
      ttcgcaaga ttattacgg aaacacgcc tctctatta 
      tgcatgcgt tcgaaatga cgccaaaat tgtcgaagt 
      attgctcac aaacggagc caatgccga tattaggga 
      ttatggaaa ctcgataat aaaacaagt ccaagaaaa 
      tctcaccga gagccagga tattttctt gtgaaataa 
      aattttata cacttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY