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Gene information
General information
TC005408 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid58: 396668..404631
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005408
    iBeetle screen iB_00851
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005408 RA
      MSDEVAENG HSEGDVPEI ELIIRASTI DGRRKGACL 
      FCQEYFMEL YLLAELKTI SLKVTTVDM QKPPPDFRT 
      NFEATPPPI LIDRGMAIL ENEKIERHI MKSVPGGHN 
      LFVQDKEVA TLIENLYTK FKMYVTKFE STDTKEASQ 
      PLLSHLERI NDFLAKRGT RFLTGDTMS CFDCELMPR 
      LQHIRIGAK AFRKFEIPT RFTALWTYM ANMYELEAF 
      RQSCPADQD IISHIKNQL GLRTTARIE LETPVFTTS 
      IPIVAET
    CDS sequence
    • >TC005408 RA
      atgtcggac gaagtagca gaaaacggt cattctgag 
      ggagacgtc cccgaaatc gagctcatc attcgtgct 
      tcaacgata gatggccgc cgcaaaggc gcctgtctc 
      ttctgccag gaatatttc atggaactc tacctcttg 
      gccgaactg aaaacaatc agtctaaag gtgacaaca 
      gtcgatatg caaaaaccg ccacccgac ttccgcacc 
      aatttcgag gcaacgcca cctcccatt ctaatcgac 
      cgaggaatg gcaattttg gagaacgaa aaaatcgaa 
      cgacatatc atgaagagc gtccccggc ggtcataac 
      ttattcgtg caagacaag gaagtggca actttgatc 
      gaaaacctc tacaccaag ttcaaaatg tacgtaacc 
      aagttcgaa tcgactgac acgaaagag gcaagtcag 
      ccgctcctt tcgcacttg gaaagaatc aacgacttc 
      ctggcgaag agagggacc aggtttctg accggcgat 
      actatgagt tgtttcgat tgcgaattg atgccgaga 
      cttcaacat atccggatc ggagcgaaa gccttccgg 
      aaattcgaa attccgacc agatttacc gcactctgg 
      acctacatg gcgaacatg tacgagttg gaggcgttc 
      agacagagc tgtcccgcc gatcaggac atcattagt 
      catattaag aaccaactg gggctcaga acaacggca 
      cggatagag ctcgagacg cctgttttt accacttct 
      attcccatt gttgctgag acataa
    mRNA sequence
    • >TC005408 RA
      atgttttac acaaataga atccaaatg tcatcgata 
      tctgaaggg gctgcctgt caatatagc ccgtccttg 
      ccggcctcc aactgtcaa ctcaatttt tcccattga 
      gtcacaaga cagcaaagt tgcagagaa gtggtgcta 
      gtgctagaa atcgtgtcc caagccgac ccagtgcct 
      tttgacgaa gtcctccac cgtagaaat gtcggacga 
      agtagcaga aaacggtca ttctgaggg agacgtccc 
      cgaaatcga gctcatcat tcgtgcttc aacgataga 
      tggccgccg caaaggcgc ctgtctctt ctgccagga 
      atatttcat ggaactcta cctcttggc cgaactgaa 
      aacaatcag tctaaaggt gacaacagt cgatatgca 
      aaaaccgcc acccgactt ccgcaccaa tttcgaggc 
      aacgccacc tcccattct aatcgaccg aggaatggc 
      aattttgga gaacgaaaa aatcgaacg acatatcat 
      gaagagcgt ccccggcgg tcataactt attcgtgca 
      agacaagga agtggcaac tttgatcga aaacctcta 
      caccaagtt caaaatgta cgtaaccaa gttcgaatc 
      gactgacac gaaagaggc aagtcagcc gctcctttc 
      gcacttgga aagaatcaa cgacttcct ggcgaagag 
      agggaccag gtttctgac cggcgatac tatgagttg 
      tttcgattg cgaattgat gccgagact tcaacatat 
      ccggatcgg agcgaaagc cttccggaa attcgaaat 
      tccgaccag atttaccgc actctggac ctacatggc 
      gaacatgta cgagttgga ggcgttcag acagagctg 
      tcccgccga tcaggacat cattagtca tattaagaa 
      ccaactggg gctcagaac aacggcacg gatagagct 
      cgagacgcc tgtttttac cacttctat tcccattgt 
      tgctgagac ataatcatc gagggcagg tggcaaggg 
      gcgatttag tacaaaatt atcaccggt aggtgtcgc 
      tagccttgc gcgtgcgct tgcagcgag gcacaaact 
      gtggctcct ggcgccctt ttcttaaaa aaaaactat 
      tgttgtata gttttttcc gaaaagggc gctcggtcc 
      cgagcgttt tgtgccccc tgtgcattt ttgcgaact 
      gtttttgat actaaaggc tttttcttg ttgaacagt 
      attgttgca aaattttga gctggactt gaattttta 
      atccgcatt atctttaag ttttataaa gttacttaa 
      aaatgttta tcatttgta tcgatttag ctaagtatg 
      gatcataat tatgttaca ttgtatttt tacgtaata 
      aaggtaact gtgaacgta gcactttgt tgttataat 
      aaataacaa tacaaaaa