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Gene information
General information
TC005406 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid58: 493961..497223
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005406
    iBeetle screen iB_00850
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005406 RA
      MSDKTNGFH DDFEEVRVP VPWGHISGK WWGPRSSQP 
      VLAIHGWQD NAGTFDTLA PLLASKGHS ILCIDLPGH 
      GLSSHLADG HYYYLFWDG IHIVRRIVK HFNWRPVTL 
      MGHSLGGGI AFLYAGTYP QEVAKYISF DIASPSVRD 
      PAKIIASIP DCVDKFLKY ETLTPEQMP CYKYNDMID 
      IVEEAYKGG VTRASCEIM MRRGMRPAG HKKDHYVFN 
      RDPRLKVAA LAFMTPDQV LEFAARTTC RVLNIRGNP 
      GMKFDFPEF YDKVLDAIG GKVEKHVVE GSHHLHLNN 
      PERVVDIVD RFLST
    CDS sequence
    • >TC005406 RA
      atgagtgat aagacaaat gggtttcac gacgatttt 
      gaggaagtt cgggttccg gtgccctgg gggcacatc 
      agtggtaaa tggtggggc cccaggtcc agccagccc 
      gtgctggcc atccacggc tggcaagac aacgccggc 
      accttcgac actttagcc cccttgctg gcgtccaaa 
      ggccactcg atcctctgc atcgacctg ccaggccac 
      ggtttgtcc tcccacctg gccgacggc cactactac 
      tacctcttc tgggacggc atccacatc gtccgccgc 
      atcgtcaag cacttcaac tggcgcccc gtcaccctc 
      atggggcac tccctcggc gggggcatc gccttcctc 
      tacgccggc acctacccc caggaggtg gccaagtac 
      atcagcttc gatattgcg agtccgagt gtgcgagac 
      cccgccaaa atcatcgcc agcatcccc gactgtgtt 
      gacaaattc ctaaaatac gagactcta acccccgaa 
      caaatgccc tgttacaaa tacaacgat atgattgat 
      attgtggag gaggcctat aaagggggc gtcaccaga 
      gcctcctgc gagattatg atgaggcga gggatgcgg 
      ccggcgggg cacaagaaa gaccactac gtttttaat 
      cgagatccg aggcttaaa gtggcagcg ctggcgttt 
      atgacgcct gatcaagtg ctggagttt gccgcgcgg 
      acgacgtgt cgcgttttg aatattcgg gggaatcct 
      gggatgaag tttgatttt ccggagttt tatgataag 
      gtgcttgat gcgattggt gggaaggtg gagaagcac 
      gtggttgag ggcagtcat cacttgcat ttaaataac 
      ccagaacgg gttgttgat attgttgat aggtttcta 
      tcaacgtaa
    mRNA sequence
    • >TC005406 RA
      gttcaggtg gggtttgaa tgagttgaa atcgtggct 
      gggtgggaa aaattgtca ttgatcgtc gaaatgagt 
      gataagaca aatgggttt cacgacgat tttgaggaa 
      gttcgggtt ccggtgccc tgggggcac atcagtggt 
      aaatggtgg ggccccagg tccagccag cccgtgctg 
      gccatccac ggctggcaa gacaacgcc ggcaccttc 
      gacacttta gcccccttg ctggcgtcc aaaggccac 
      tcgatcctc tgcatcgac ctgccaggc cacggtttg 
      tcctcccac ctggccgac ggccactac tactacctc 
      ttctgggac ggcatccac atcgtccgc cgcatcgtc 
      aagcacttc aactggcgc cccgtcacc ctcatgggg 
      cactccctc ggcgggggc atcgccttc ctctacgcc 
      ggcacctac ccccaggag gtggccaag tacatcagc 
      ttcgatatt gcgagtccg agtgtgcga gaccccgcc 
      aaaatcatc gccagcatc cccgactgt gttgacaaa 
      ttcctaaaa tacgagact ctaaccccc gaacaaatg 
      ccctgttac aaatacaac gatatgatt gatattgtg 
      gaggaggcc tataaaggg ggcgtcacc agagcctcc 
      tgcgagatt atgatgagg cgagggatg cggccggcg 
      gggcacaag aaagaccac tacgttttt aatcgagat 
      ccgaggctt aaagtggca gcgctggcg tttatgacg 
      cctgatcaa gtgctggag tttgccgcg cggacgacg 
      tgtcgcgtt ttgaatatt cgggggaat cctgggatg 
      aagtttgat tttccggag ttttatgat aaggtgctt 
      gatgcgatt ggtgggaag gtggagaag cacgtggtt 
      gagggcagt catcacttg catttaaat aacccagaa 
      cgggttgtt gatattgtt gataggttt ctatcaacg 
      taaagttag acggaattg tgatattta tttttctag 
      tgtcagtat tttattgtt gattctttc ttgtagttg 
      aataaaaca ctttggctt cgatttttt ctcgacgag 
      taattgctt tatttttgt taatgattc ttcacgaac 
      gtgtgaaat aaaatttcg attttttc