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Gene information
General information
TC034176
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005403
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid58: 510304..512500
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034176
    iBeetle screen iB_00848
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 5 - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034176 RA
      MSQILLKLF LFTGILTTG YCNYLKQAR DQHRLQLSS 
      KSDLRYLSS LSDPNYINK VLDKILIPR VAGTPEHEK 
      VFKFLTSEL RRLGWQVDV DEFVERAPI FSQVTFKNI 
      VASPNPQAE RFLVLACHY DSKYFPNAV FVGAIDSAV 
      PCAMMLEIA KTLSKELGR IKDSPVGLK FIFFDGEEA 
      FEEWGPNDS IWGAKHLAQ VLHANTSTA NGEIVTELD 
      KMDVLVLLD LIGMKNPKF LNFFENTQR WFVRLSQIE 
      IELHRMNLL RNHKQGYFQ SSRPYGRIE DDHLPFLRR 
      DVPVLHLIS TPFPPEWHT PRDNRNIVD MNTVNNLNL 
      ILKIFISEY LQLLV
    • >TC034176 RB
      MSQILLKLF LFTGILTTG YCNYLKQAR DQHRLQLSS 
      KSDLRYLSS LSDPNYINK VLDKILIPR VAGTPEHEK 
      VFKFLTSEL RRLGWQVDV DEFVERAPI FSQVTFKNI 
      VASPNPQAE RFLVLACHY DSKYFPNAV FVGAIDSAV 
      PCAMMLEIA KTLSKELGR IKDSPVGLK FIFFDGEEA 
      FEEWGPNDS IWGAKHLAQ VLHANTSTA NGEIVTELD 
      KMDVLVLLD LIGMKNPKF LNFFENTQR WFVRLSQIE 
      IELHRMNLL RNHKQGYFQ SSRPYGRIE DDHLPFLRR 
      DVPVLHLIS TPFPPEWHT PRDNRNIVD MNTVNNLNL 
      ILKIFISEY LQLSLAESA EELPEKEL
    CDS sequence
    • >TC034176 RA
      atgagccaa attttacta aaacttttc ctattcacg 
      ggtatactc acaaccgga tattgcaat tacctgaaa 
      caagcccgc gaccaacac aggctgcaa ttatcgtca 
      aaaagcgac ttgcgctac ttatcctcc ctctccgac 
      ccaaattac atcaacaaa gtgctcgat aaaatccta 
      attccaaga gtagcggga acccccgag cacgaaaaa 
      gtcttcaag ttcctgacc tcggaactg cgccgattg 
      gggtggcaa gtggacgtc gacgagttc gtcgaacgc 
      gcccccatt ttcagccaa gtcacgttc aaaaatatc 
      gtggcaagc cccaacccc caggctgaa cgtttcctg 
      gttttggcc tgtcattat gactctaaa tattttcca 
      aacgccgtt tttgtgggc gctattgat tctgcagtg 
      ccctgtgct atgatgctc gaaatcgcc aaaacccta 
      tcgaaagag ttggggcgt attaaagat tcgccagtt 
      ggcttgaaa tttattttt ttcgacggt gaggaggcg 
      tttgaagag tggggcccc aatgactcg atttggggg 
      gcgaagcat ctggcacag gtgctccac gcgaacacg 
      agcacggca aatggggaa atcgtgacc gaattggat 
      aaaatggac gttttagtt ttgctagac ttgattggg 
      atgaaaaat cccaagttt ttgaatttt tttgaaaac 
      acccagcga tggtttgtg aggttgtcg caaattgag 
      attgagttg cacaggatg aaccttttg aggaaccac 
      aaacagggc tattttcag tcaagtagg ccgtatggg 
      aggattgag gacgaccat ctcccgttt ttgaggagg 
      gatgtgcca gtgctacat ttgattagc actccattc 
      cctcccgaa tggcacact ccgcgtgat aatcgcaat 
      atcgttgac atgaacact gtcaataat ctcaacttg 
      atattgaaa atttttatt agcgaatat ttacagctg 
      ttagtataa
    • >TC034176 RB
      atgagccaa attttacta aaacttttc ctattcacg 
      ggtatactc acaaccgga tattgcaat tacctgaaa 
      caagcccgc gaccaacac aggctgcaa ttatcgtca 
      aaaagcgac ttgcgctac ttatcctcc ctctccgac 
      ccaaattac atcaacaaa gtgctcgat aaaatccta 
      attccaaga gtagcggga acccccgag cacgaaaaa 
      gtcttcaag ttcctgacc tcggaactg cgccgattg 
      gggtggcaa gtggacgtc gacgagttc gtcgaacgc 
      gcccccatt ttcagccaa gtcacgttc aaaaatatc 
      gtggcaagc cccaacccc caggctgaa cgtttcctg 
      gttttggcc tgtcattat gactctaaa tattttcca 
      aacgccgtt tttgtgggc gctattgat tctgcagtg 
      ccctgtgct atgatgctc gaaatcgcc aaaacccta 
      tcgaaagag ttggggcgt attaaagat tcgccagtt 
      ggcttgaaa tttattttt ttcgacggt gaggaggcg 
      tttgaagag tggggcccc aatgactcg atttggggg 
      gcgaagcat ctggcacag gtgctccac gcgaacacg 
      agcacggca aatggggaa atcgtgacc gaattggat 
      aaaatggac gttttagtt ttgctagac ttgattggg 
      atgaaaaat cccaagttt ttgaatttt tttgaaaac 
      acccagcga tggtttgtg aggttgtcg caaattgag 
      attgagttg cacaggatg aaccttttg aggaaccac 
      aaacagggc tattttcag tcaagtagg ccgtatggg 
      aggattgag gacgaccat ctcccgttt ttgaggagg 
      gatgtgcca gtgctacat ttgattagc actccattc 
      cctcccgaa tggcacact ccgcgtgat aatcgcaat 
      atcgttgac atgaacact gtcaataat ctcaacttg 
      atattgaaa atttttatt agcgaatat ttacagctt 
      tccttggcg gagtcggcg gaagaacta ccggaaaag 
      gagttgtga
    mRNA sequence
    • >TC034176 RA
      tacgagtta gcctctgcg ttattcaaa tttaattcc 
      tgctgtctt tcagtagtg tagtgttta agtgtagtg 
      ttgtgtctt ttggttcaa aaagtgcgc cgttttagt 
      gacgttagt gtgacattt gccacattg gcagtcata 
      aaatttagg ttaggctcc tgtcaaaac catatgttt 
      ttccagtat tatcaagta aattaacaa aaaatgagc 
      caaatttta ctaaaactt ttcctattc acgggtata 
      ctcacaacc ggatattgc aattacctg aaacaagcc 
      cgcgaccaa cacaggctg caattatcg tcaaaaagc 
      gacttgcgc tacttatcc tccctctcc gacccaaat 
      tacatcaac aaagtgctc gataaaatc ctaattcca 
      agagtagcg ggaaccccc gagcacgaa aaagtcttc 
      aagttcctg acctcggaa ctgcgccga ttggggtgg 
      caagtggac gtcgacgag ttcgtcgaa cgcgccccc 
      attttcagc caagtcacg ttcaaaaat atcgtggca 
      agccccaac ccccaggct gaacgtttc ctggttttg 
      gcctgtcat tatgactct aaatatttt ccaaacgcc 
      gtttttgtg ggcgctatt gattctgca gtgccctgt 
      gctatgatg ctcgaaatc gccaaaacc ctatcgaaa 
      gagttgggg cgtattaaa gattcgcca gttggcttg 
      aaatttatt tttttcgac ggtgaggag gcgtttgaa 
      gagtggggc cccaatgac tcgatttgg ggggcgaag 
      catctggca caggtgctc cacgcgaac acgagcacg 
      gcaaatggg gaaatcgtg accgaattg gataaaatg 
      gacgtttta gttttgcta gacttgatt gggatgaaa 
      aatcccaag tttttgaat ttttttgaa aacacccag 
      cgatggttt gtgaggttg tcgcaaatt gagattgag 
      ttgcacagg atgaacctt ttgaggaac cacaaacag 
      ggctatttt cagtcaagt aggccgtat gggaggatt 
      gaggacgac catctcccg tttttgagg agggatgtg 
      ccagtgcta catttgatt agcactcca ttccctccc 
      gaatggcac actccgcgt gataatcgc aatatcgtt 
      gacatgaac actgtcaat aatctcaac ttgatattg 
      aaaattttt attagcgaa tatttacag ctgttagta 
      taatttatt atttttttt agttgggta aaaagaaat 
      tttgtgggg gttttattt attgtatgc atcaagtag 
      gtcatgatt gacgtaaca attgtcatt tttttaaag 
      cctactttg atattaaac gcaaaaatt ctattttcc 
      caacgtgtg tctgtctga tcaatgcat aattaccaa 
      ctaacaatt cattgatat tttaaatag ttccttggc 
      ggagtcggc ggaagaact accggaaaa ggagttgtg 
      atacatcaa agactcaat ttattatgc attgttgcg 
      aaaaatttt ttgtggagt ctacttaac tattagata 
      accattttt cgtatttta cagatatgt taaacctga 
      cgacaagac tcgcgaaaa tcccatccc tttgatcac 
      tgtctttgt atttgcact aatttgtat tatttacaa 
      aaatttaca gcgaaaata acttgggtg ctttaaaac 
      ccacccagt acacaacag tgtattact ggttgcaga 
      atcaaagtc aattgattt atcaggaat aaaaccgaa 
      tttacactc ttgataaat gtaaatagt attaaatat 
      ttttttacg gaaattagg aattttgca atatttatt 
      ttttattta atttattgt tgtatcgta tgtaataaa 
      aaaaatata atttgttga agcagaacc tcgtatttg 
      ttgacctga aacagaatc ctggaaaaa cgtcaagga 
      acgataagt tcgcactaa cagttgtgt ccatctaac 
      ttctaacta attaaaaaa tcgaataaa tagctcaaa 
      tattt
    • >TC034176 RB
      tacgagtta gcctctgcg ttattcaaa tttaattcc 
      tgctgtctt tcagtagtg tagtgttta agtgtagtg 
      ttgtgtctt ttggttcaa aaagtgcgc cgttttagt 
      gacgttagt gtgacattt gccacattg gcagtcata 
      aaatttagg ttaggctcc tgtcaaaac catatgttt 
      ttccagtat tatcaagta aattaacaa aaaatgagc 
      caaatttta ctaaaactt ttcctattc acgggtata 
      ctcacaacc ggatattgc aattacctg aaacaagcc 
      cgcgaccaa cacaggctg caattatcg tcaaaaagc 
      gacttgcgc tacttatcc tccctctcc gacccaaat 
      tacatcaac aaagtgctc gataaaatc ctaattcca 
      agagtagcg ggaaccccc gagcacgaa aaagtcttc 
      aagttcctg acctcggaa ctgcgccga ttggggtgg 
      caagtggac gtcgacgag ttcgtcgaa cgcgccccc 
      attttcagc caagtcacg ttcaaaaat atcgtggca 
      agccccaac ccccaggct gaacgtttc ctggttttg 
      gcctgtcat tatgactct aaatatttt ccaaacgcc 
      gtttttgtg ggcgctatt gattctgca gtgccctgt 
      gctatgatg ctcgaaatc gccaaaacc ctatcgaaa 
      gagttgggg cgtattaaa gattcgcca gttggcttg 
      aaatttatt tttttcgac ggtgaggag gcgtttgaa 
      gagtggggc cccaatgac tcgatttgg ggggcgaag 
      catctggca caggtgctc cacgcgaac acgagcacg 
      gcaaatggg gaaatcgtg accgaattg gataaaatg 
      gacgtttta gttttgcta gacttgatt gggatgaaa 
      aatcccaag tttttgaat ttttttgaa aacacccag 
      cgatggttt gtgaggttg tcgcaaatt gagattgag 
      ttgcacagg atgaacctt ttgaggaac cacaaacag 
      ggctatttt cagtcaagt aggccgtat gggaggatt 
      gaggacgac catctcccg tttttgagg agggatgtg 
      ccagtgcta catttgatt agcactcca ttccctccc 
      gaatggcac actccgcgt gataatcgc aatatcgtt 
      gacatgaac actgtcaat aatctcaac ttgatattg 
      aaaattttt attagcgaa tatttacag ctttccttg 
      gcggagtcg gcggaagaa ctaccggaa aaggagttg 
      tgatacatc aaagactca atttattat gcattgttg 
      cgaaaaatt ttttgtgga gtctactta actattaga 
      taaccattt ttcgtattt tacagatat gttaaacct 
      gacgacaag actcgcgaa aatcccatc cctttgatc 
      actgtcttt gtatttgca ctaatttgt attatttac 
      aaaaattta cagcgaaaa taacttggg tgctttaaa 
      acccaccca gtacacaac agtgtatta ctggttgca 
      gaatcaaag tcaattgat ttatcagga ataaaaccg 
      aatttacac tcttgataa atgtaaata gtattaaat 
      attttttta cggaaatta ggaattttg caatattta 
      ttttttatt taatttatt gttgtatcg tatgtaata 
      aaaaaaata taatttg