| General information | |||
| TC034178
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 13720990..13729997 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034178 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00847 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%
embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
- >TC034178 RA
MDEENKVNF ESVENEAKS SPTFTPHSA KSSEREETN VLHPDNPLL EKFQKALKE HLLRQINHL KNDIFEYDC GTKKNNARR EELGIMVYE QQQILEKQQ KTLDLYISK VQTATAACE EMQNLVKEK KELFKKQRD KVYEAEAKE LELRNEIEA TNLLVHQIS EWEENLESD LTVKKRVFE KSRKEKLKL LEDKRTQDV LIYKLMTRI WFLERELDR MDVQMKVKE AEREDLAQT VALGNTNIE AMEAEYRCL MHSWNSVVI AIGNRDKVL SCLNKELSK SEEHFKSVV SEIEQVKKL SQKEMRRNE EFTMLKNRF LADVQVCKA ALDVEIEKK TTLEQRMFE MQRIIEQTE ADIQRITDE AHETHTILS GLTREHEQL DTQKGELEE QIMRALEEQ VTNNKMLMT LQRALTQFK IKNREIEIQ VATIENKIA SVMSEMEAQ KFANFEGQR YLKELEEQL KELGMEADR YQDEMKHIE LKIAKKQRE VDLLNNKYL KMVDKSGGT FVSPLEMKI TTLEKNIEE IQEYNKKLQ LYWLREQSN LIKISEERQ EQIHNNNLM CKQILILEQ KNLRVSDEL EACRKLQDK VVRSINNLH SQLTVMGDN FVKTKGLKI NMDKDNEVL RQDYIYKLK DAELECLKL EDEITQIEE DKVNLSNEL IAMNRETLE WEKKFLGAK ETKNTMIEE RGQEGEVGT MKAEIHRMN VRYSQLKKV QDVLVQDLE HCVSRRDAM MTAAEAREK RVKGGMEKT RINFNRKMD DMRNKSKQM ENQIAEMAK KTEHMAKEI EQIRAEIAE MSREIETTN EHCVEIREN VEEALTKRQ MNFELLLQV QKKLNTYND LAKGRKPHL VHRSESALS SAYSREIST NSKLCKIVE NLQSDFPNH GHELVRICN TLKLPVYVL GFDSLVLYK NMEGVGAYG GGKAGGAFD PISFAQRPQ VLLRAVCWL FSIVVFGCI SSQGWLINK NGKEECLYN GDANACNYG VGISVIAFL ASMGFLAGE YLFEQMSSV KTRKHYVLG DLGFSAFWA FLYFVGFWY LASQWGKSK TPENGYGVN NVQAAIAFS FFSIFSWAG CAFLAFQRF RQGADAAFA TNYEADTSM PGSYPSYPG GPDSDQHYQ EPPFSAGPN QRGPTDFQA PAY
- >TC034178 RA
atggacgag gagaataaa gtgaatttt gaaagtgta gaaaatgag gcaaaatct tccccaact tttacacca cattcggca aaatcgtcc gaaagagag gaaactaat gtcttgcac cctgacaac ccccttttg gaaaaattt caaaaagcg ctcaaggag cacttattg cgtcaaata aatcacctc aaaaacgac attttcgaa tacgattgt gggacgaaa aaaaacaat gctagacgt gaagaactg gggattatg gtttacgaa caacaacaa attttggaa aaacaacaa aaaacccta gatttgtac atttctaaa gtgcaaact gcgacagca gcctgcgaa gaaatgcaa aacctagtc aaagaaaaa aaagaactg tttaaaaaa caaagagac aaagtttac gaagccgag gctaaagaa ctcgaattg cgaaacgaa atcgaggca acaaatctt ctagttcac caaatcagc gaatgggag gagaacttg gagtcagac ttgacggta aaaaagcgc gtttttgag aaaagccgc aaggaaaag ctcaaactc ctcgaagac aaacgcact caagacgtc ctcatttac aaactaatg accaggatc tggtttctc gaacgcgaa ctggacaga atggacgtg caaatgaag gtgaaagag gcggaaagg gaggatttg gcccaaact gtggccctc gggaacaca aacatcgaa gcaatggaa gcggaatac cgctgtcta atgcactcc tggaactct gtcgttatc gcaatcgga aatcgcgat aaagtcctc tcgtgtctc aacaaagaa ctgtccaag tccgaggaa cacttcaaa agcgtggtt tccgaaatc gaacaagtc aaaaaactc tcccagaag gaaatgcgt cgcaatgag gagttcaca atgttgaag aaccgcttc ctggccgat gtccaggtg tgcaaagcg gctctggac gtcgaaatc gagaaaaaa accaccctg gaacaacgc atgttcgaa atgcaacgc attattgaa caaaccgag gccgatatt caacgcata acggacgaa gcgcacgaa acacacacc atactttcg gggctgacg cgcgaacac gaacaactc gacacgcaa aaaggcgaa ctcgaggag caaataatg cgagctttg gaggaacaa gtcactaat aataaaatg ctgatgacg ctacaacgc gcattgaca caattcaaa atcaaaaat cgggaaatt gaaatacaa gtcgccacg attgagaat aaaatcgca tcggtgatg agcgaaatg gaagcgcag aaatttgca aattttgag ggacaacgg tatttgaag gagttggaa gagcagttg aaggagttg ggaatggag gcggaccgc taccaggac gagatgaaa catattgag cttaagatt gcaaagaaa cagcgggaa gttgatttg ttgaataac aagtatttg aaaatggtg gataagtcg ggcgggacg tttgtctcg ccccttgaa atgaaaata acaacactg gagaaaaat atcgaggag atacaagag tacaacaag aaattgcaa ttgtattgg ctgcgggaa cagtccaac ttgattaaa atctcggaa gaacggcag gaacaaatc cacaataat aatctcatg tgtaaacag attttgatc ctggagcag aagaatttg agggtttcg gacgagctt gaagcttgt cgcaagtta caagataaa gtcgttcgc agtataaat aatctccac agtcaattg accgtcatg ggtgataat ttcgtcaag actaagggt ttgaagata aatatggat aaagataac gaagtgttg cgtcaggat tatatctac aagcttaag gatgccgag cttgaatgc ctcaaattg gaggatgag attacgcaa attgaggag gataaggtg aatttgagt aatgaattg attgcaatg aatcgtgaa actctcgaa tgggagaag aagtttttg ggggcgaag gaaacgaag aatacgatg attgaggaa agggggcag gaaggggag gttgggacg atgaaagcg gagatacat cgcatgaat gtgcgctat agccaattg aagaaggtc caggatgtc ttggtccaa gacttggag cactgcgtt tcgcgaaga gacgcaatg atgacagct gcggaagca cgcgaaaaa cgcgtaaaa ggcggaatg gaaaaaact cgtattaat ttcaaccgc aaaatggac gacatgcga aacaaatcg aaacaaatg gaaaatcaa attgcggaa atggccaaa aaaaccgaa cacatggcc aaagaaatt gagcaaatc cgggccgaa atcgccgaa atgtcgcgc gagattgag acgacaaac gagcattgt gtcgaaatt cgcgaaaat gttgaagag gccttgacg aaacggcaa atgaacttc gagttgttg ctccaagtg cagaaaaaa ctcaacact tataatgac ctggccaag gggcgcaag ccccacttg gtgcaccgc tcggaaagc gccttgtcc tcggcctac tctagggaa atttccacc aattctaaa ctatgcaag attgtggaa aacctccag tcggacttt cccaaccat gggcacgag ctggtccgc atttgtaac acgctgaaa ctgcccgtt tatgttctt ggttttgac tcattagta ctttacaaa aacatggag ggtgtagga gcgtacgga ggcggtaaa gcaggaggt gctttcgac ccgatttcc ttcgctcag cggccccaa gtgctcctg agggccgtc tgctggctg ttctccatc gtggtgttc ggctgtata tcgtcccaa ggctggcta atcaacaaa aacggcaaa gaggagtgt ctgtataac ggggacgcg aacgcgtgc aattacggg gttggtatc agtgtgatt gccttcctg gccagcatg gggttcctg gccggggaa tacctcttc gagcaaatg tcgtcggtc aagacacgg aagcactac gttctgggc gacttggga ttctcagca ttttgggcg ttcctgtat tttgtgggc ttttggtac ctcgccagc caatggggt aagtccaag acgccggaa aacggctac ggcgtgaac aacgtccag gccgcaatc gccttttcc ttcttttcg attttctcc tgggcgggt tgcgccttt ttggcgttc cagcgtttc cggcagggc gctgatgca gcatttgcc accaactac gaggccgat acgagtatg cctggctcg tatccgtct tatcctggg ggccccgat tcagaccaa cactaccag gagccccca ttttccgcc ggtcctaac caacgagga ccgacggat ttccaagcg ccagcttat taa
- >TC034178 RA
cttgaactt ttttaaaaa tcgataatt tcggtggcg tacttcccc gcgagttta agcacgttg ctaggcgac taatagcaa aacataaac aacttggca gtgacagtc cagtgacag tgtctttat ttactgaca aataaaaat ggacgagga gaataaagt gaattttga aagtgtaga aaatgaggc aaaatcttc cccaacttt tacaccaca ttcggcaaa atcgtccga aagagagga aactaatgt cttgcaccc tgacaaccc ccttttgga aaaatttca aaaagcgct caaggagca cttattgcg tcaaataaa tcacctcaa aaacgacat tttcgaata cgattgtgg gacgaaaaa aaacaatgc tagacgtga agaactggg gattatggt ttacgaaca acaacaaat tttggaaaa acaacaaaa aaccctaga tttgtacat ttctaaagt gcaaactgc gacagcagc ctgcgaaga aatgcaaaa cctagtcaa agaaaaaaa agaactgtt taaaaaaca aagagacaa agtttacga agccgaggc taaagaact cgaattgcg aaacgaaat cgaggcaac aaatcttct agttcacca aatcagcga atgggagga gaacttgga gtcagactt gacggtaaa aaagcgcgt ttttgagaa aagccgcaa ggaaaagct caaactcct cgaagacaa acgcactca agacgtcct catttacaa actaatgac caggatctg gtttctcga acgcgaact ggacagaat ggacgtgca aatgaaggt gaaagaggc ggaaaggga ggatttggc ccaaactgt ggccctcgg gaacacaaa catcgaagc aatggaagc ggaataccg ctgtctaat gcactcctg gaactctgt cgttatcgc aatcggaaa tcgcgataa agtcctctc gtgtctcaa caaagaact gtccaagtc cgaggaaca cttcaaaag cgtggtttc cgaaatcga acaagtcaa aaaactctc ccagaagga aatgcgtcg caatgagga gttcacaat gttgaagaa ccgcttcct ggccgatgt ccaggtgtg caaagcggc tctggacgt cgaaatcga gaaaaaaac caccctgga acaacgcat gttcgaaat gcaacgcat tattgaaca aaccgaggc cgatattca acgcataac ggacgaagc gcacgaaac acacaccat actttcggg gctgacgcg cgaacacga acaactcga cacgcaaaa aggcgaact cgaggagca aataatgcg agctttgga ggaacaagt cactaataa taaaatgct gatgacgct acaacgcgc attgacaca attcaaaat caaaaatcg ggaaattga aatacaagt cgccacgat tgagaataa aatcgcatc ggtgatgag cgaaatgga agcgcagaa atttgcaaa ttttgaggg acaacggta tttgaagga gttggaaga gcagttgaa ggagttggg aatggaggc ggaccgcta ccaggacga gatgaaaca tattgagct taagattgc aaagaaaca gcgggaagt tgatttgtt gaataacaa gtatttgaa aatggtgga taagtcggg cgggacgtt tgtctcgcc ccttgaaat gaaaataac aacactgga gaaaaatat cgaggagat acaagagta caacaagaa attgcaatt gtattggct gcgggaaca gtccaactt gattaaaat ctcggaaga acggcagga acaaatcca caataataa tctcatgtg taaacagat tttgatcct ggagcagaa gaatttgag ggtttcgga cgagcttga agcttgtcg caagttaca agataaagt cgttcgcag tataaataa tctccacag tcaattgac cgtcatggg tgataattt cgtcaagac taagggttt gaagataaa tatggataa agataacga agtgttgcg tcaggatta tatctacaa gcttaagga tgccgagct tgaatgcct caaattgga ggatgagat tacgcaaat tgaggagga taaggtgaa tttgagtaa tgaattgat tgcaatgaa tcgtgaaac tctcgaatg ggagaagaa gtttttggg ggcgaagga aacgaagaa tacgatgat tgaggaaag ggggcagga aggggaggt tgggacgat gaaagcgga gatacatcg catgaatgt gcgctatag ccaattgaa gaaggtcca ggatgtctt ggtccaaga cttggagca ctgcgtttc gcgaagaga cgcaatgat gacagctgc ggaagcacg cgaaaaacg cgtaaaagg cggaatgga aaaaactcg tattaattt caaccgcaa aatggacga catgcgaaa caaatcgaa acaaatgga aaatcaaat tgcggaaat ggccaaaaa aaccgaaca catggccaa agaaattga gcaaatccg ggccgaaat cgccgaaat gtcgcgcga gattgagac gacaaacga gcattgtgt cgaaattcg cgaaaatgt tgaagaggc cttgacgaa acggcaaat gaacttcga gttgttgct ccaagtgca gaaaaaact caacactta taatgacct ggccaaggg gcgcaagcc ccacttggt gcaccgctc ggaaagcgc cttgtcctc ggcctactc tagggaaat ttccaccaa ttctaaact atgcaagat tgtggaaaa cctccagtc ggactttcc caaccatgg gcacgagct ggtccgcat ttgtaacac gctgaaact gcccgttta tgttcttgg ttttgactc attagtact ttacaaaaa catggaggg tgtaggagc gtacggagg cggtaaagc aggaggtgc tttcgaccc gatttcctt cgctcagcg gccccaagt gctcctgag ggccgtctg ctggctgtt ctccatcgt ggtgttcgg ctgtatatc gtcccaagg ctggctaat caacaaaaa cggcaaaga ggagtgtct gtataacgg ggacgcgaa cgcgtgcaa ttacggggt tggtatcag tgtgattgc cttcctggc cagcatggg gttcctggc cggggaata cctcttcga gcaaatgtc gtcggtcaa gacacggaa gcactacgt tctgggcga cttgggatt ctcagcatt ttgggcgtt cctgtattt tgtgggctt ttggtacct cgccagcca atggggtaa gtccaagac gccggaaaa cggctacgg cgtgaacaa cgtccaggc cgcaatcgc cttttcctt cttttcgat tttctcctg ggcgggttg cgccttttt ggcgttcca gcgtttccg gcagggcgc tgatgcagc atttgccac caactacga ggccgatac gagtatgcc tggctcgta tccgtctta tcctggggg ccccgattc agaccaaca ctaccagga gcccccatt ttccgccgg tcctaacca acgaggacc gacggattt ccaagcgcc agcttatta actgggccc ccaattctc ataatatag tgaacatag cgcagctgc ctcatctct aaagcgagt cgaaaactc gccgacatt ttcagtata taataaaag tcctttgta cttagaatc gtacggaaa caaggtttt ggttttcag tattatttg gatgaacct caaggagtg ttgttacgt tgcgttttt gtaaagccg tgtacttat agtagctac ttatatcac acacaaaac ttcaaaatg tgttgaatt ataaaaatg ttacataat tatatagaa tgttcaatg tacagttat ataataatt gtcgagttg cttgtagcg agttttgct ttttttttc gaagcaatt ttaggcgtt tctgtgcta gtagagtca gtgtgactc gaccgattg gcgcagtcg gtaggatcg agtcgttgc acttttatt attttttta accagtgcc attttttat gttttgata atgtgggat caacttgtt tgcgaagcg attgttatt aacagtttt ttgttgagg ttgagcgac aataaataa gcaattcac gggcactaa ggtggcaaa aaaaacata ttgacagat tttacatat ctggagcac aattttcta acaattggt acgtctcat taaatactc aaaatttaa aatgctgct tcgattatg atgaatttt attatctaa gtgggtgtc gcatagtaa agttgttcc ttgtcacgg aaataggtc attagcata atttaatta gcaataaaa gacaactgt agatatgtt tcattatct cggttcggg atttggttt cgttcaagt taacgtgaa ttgatataa agactatta tttataatt atttttcta ttgaaatgt gatttttat ttttatgga atattatat ttaggtgga cgaattgat tcaatgtat taataagtt accaataaa tg
