Status
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Gene information
General information
TC034178
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005401,TC005400
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid58: 534195..543202
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034178
    iBeetle screen iB_00847
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034178 RA
      MDEENKVNF ESVENEAKS SPTFTPHSA KSSEREETN 
      VLHPDNPLL EKFQKALKE HLLRQINHL KNDIFEYDC 
      GTKKNNARR EELGIMVYE QQQILEKQQ KTLDLYISK 
      VQTATAACE EMQNLVKEK KELFKKQRD KVYEAEAKE 
      LELRNEIEA TNLLVHQIS EWEENLESD LTVKKRVFE 
      KSRKEKLKL LEDKRTQDV LIYKLMTRI WFLERELDR 
      MDVQMKVKE AEREDLAQT VALGNTNIE AMEAEYRCL 
      MHSWNSVVI AIGNRDKVL SCLNKELSK SEEHFKSVV 
      SEIEQVKKL SQKEMRRNE EFTMLKNRF LADVQVCKA 
      ALDVEIEKK TTLEQRMFE MQRIIEQTE ADIQRITDE 
      AHETHTILS GLTREHEQL DTQKGELEE QIMRALEEQ 
      VTNNKMLMT LQRALTQFK IKNREIEIQ VATIENKIA 
      SVMSEMEAQ KFANFEGQR YLKELEEQL KELGMEADR 
      YQDEMKHIE LKIAKKQRE VDLLNNKYL KMVDKSGGT 
      FVSPLEMKI TTLEKNIEE IQEYNKKLQ LYWLREQSN 
      LIKISEERQ EQIHNNNLM CKQILILEQ KNLRVSDEL 
      EACRKLQDK VVRSINNLH SQLTVMGDN FVKTKGLKI 
      NMDKDNEVL RQDYIYKLK DAELECLKL EDEITQIEE 
      DKVNLSNEL IAMNRETLE WEKKFLGAK ETKNTMIEE 
      RGQEGEVGT MKAEIHRMN VRYSQLKKV QDVLVQDLE 
      HCVSRRDAM MTAAEAREK RVKGGMEKT RINFNRKMD 
      DMRNKSKQM ENQIAEMAK KTEHMAKEI EQIRAEIAE 
      MSREIETTN EHCVEIREN VEEALTKRQ MNFELLLQV 
      QKKLNTYND LAKGRKPHL VHRSESALS SAYSREIST 
      NSKLCKIVE NLQSDFPNH GHELVRICN TLKLPVYVL 
      GFDSLVLYK NMEGVGAYG GGKAGGAFD PISFAQRPQ 
      VLLRAVCWL FSIVVFGCI SSQGWLINK NGKEECLYN 
      GDANACNYG VGISVIAFL ASMGFLAGE YLFEQMSSV 
      KTRKHYVLG DLGFSAFWA FLYFVGFWY LASQWGKSK 
      TPENGYGVN NVQAAIAFS FFSIFSWAG CAFLAFQRF 
      RQGADAAFA TNYEADTSM PGSYPSYPG GPDSDQHYQ 
      EPPFSAGPN QRGPTDFQA PAY
    CDS sequence
    • >TC034178 RA
      atggacgag gagaataaa gtgaatttt gaaagtgta 
      gaaaatgag gcaaaatct tccccaact tttacacca 
      cattcggca aaatcgtcc gaaagagag gaaactaat 
      gtcttgcac cctgacaac ccccttttg gaaaaattt 
      caaaaagcg ctcaaggag cacttattg cgtcaaata 
      aatcacctc aaaaacgac attttcgaa tacgattgt 
      gggacgaaa aaaaacaat gctagacgt gaagaactg 
      gggattatg gtttacgaa caacaacaa attttggaa 
      aaacaacaa aaaacccta gatttgtac atttctaaa 
      gtgcaaact gcgacagca gcctgcgaa gaaatgcaa 
      aacctagtc aaagaaaaa aaagaactg tttaaaaaa 
      caaagagac aaagtttac gaagccgag gctaaagaa 
      ctcgaattg cgaaacgaa atcgaggca acaaatctt 
      ctagttcac caaatcagc gaatgggag gagaacttg 
      gagtcagac ttgacggta aaaaagcgc gtttttgag 
      aaaagccgc aaggaaaag ctcaaactc ctcgaagac 
      aaacgcact caagacgtc ctcatttac aaactaatg 
      accaggatc tggtttctc gaacgcgaa ctggacaga 
      atggacgtg caaatgaag gtgaaagag gcggaaagg 
      gaggatttg gcccaaact gtggccctc gggaacaca 
      aacatcgaa gcaatggaa gcggaatac cgctgtcta 
      atgcactcc tggaactct gtcgttatc gcaatcgga 
      aatcgcgat aaagtcctc tcgtgtctc aacaaagaa 
      ctgtccaag tccgaggaa cacttcaaa agcgtggtt 
      tccgaaatc gaacaagtc aaaaaactc tcccagaag 
      gaaatgcgt cgcaatgag gagttcaca atgttgaag 
      aaccgcttc ctggccgat gtccaggtg tgcaaagcg 
      gctctggac gtcgaaatc gagaaaaaa accaccctg 
      gaacaacgc atgttcgaa atgcaacgc attattgaa 
      caaaccgag gccgatatt caacgcata acggacgaa 
      gcgcacgaa acacacacc atactttcg gggctgacg 
      cgcgaacac gaacaactc gacacgcaa aaaggcgaa 
      ctcgaggag caaataatg cgagctttg gaggaacaa 
      gtcactaat aataaaatg ctgatgacg ctacaacgc 
      gcattgaca caattcaaa atcaaaaat cgggaaatt 
      gaaatacaa gtcgccacg attgagaat aaaatcgca 
      tcggtgatg agcgaaatg gaagcgcag aaatttgca 
      aattttgag ggacaacgg tatttgaag gagttggaa 
      gagcagttg aaggagttg ggaatggag gcggaccgc 
      taccaggac gagatgaaa catattgag cttaagatt 
      gcaaagaaa cagcgggaa gttgatttg ttgaataac 
      aagtatttg aaaatggtg gataagtcg ggcgggacg 
      tttgtctcg ccccttgaa atgaaaata acaacactg 
      gagaaaaat atcgaggag atacaagag tacaacaag 
      aaattgcaa ttgtattgg ctgcgggaa cagtccaac 
      ttgattaaa atctcggaa gaacggcag gaacaaatc 
      cacaataat aatctcatg tgtaaacag attttgatc 
      ctggagcag aagaatttg agggtttcg gacgagctt 
      gaagcttgt cgcaagtta caagataaa gtcgttcgc 
      agtataaat aatctccac agtcaattg accgtcatg 
      ggtgataat ttcgtcaag actaagggt ttgaagata 
      aatatggat aaagataac gaagtgttg cgtcaggat 
      tatatctac aagcttaag gatgccgag cttgaatgc 
      ctcaaattg gaggatgag attacgcaa attgaggag 
      gataaggtg aatttgagt aatgaattg attgcaatg 
      aatcgtgaa actctcgaa tgggagaag aagtttttg 
      ggggcgaag gaaacgaag aatacgatg attgaggaa 
      agggggcag gaaggggag gttgggacg atgaaagcg 
      gagatacat cgcatgaat gtgcgctat agccaattg 
      aagaaggtc caggatgtc ttggtccaa gacttggag 
      cactgcgtt tcgcgaaga gacgcaatg atgacagct 
      gcggaagca cgcgaaaaa cgcgtaaaa ggcggaatg 
      gaaaaaact cgtattaat ttcaaccgc aaaatggac 
      gacatgcga aacaaatcg aaacaaatg gaaaatcaa 
      attgcggaa atggccaaa aaaaccgaa cacatggcc 
      aaagaaatt gagcaaatc cgggccgaa atcgccgaa 
      atgtcgcgc gagattgag acgacaaac gagcattgt 
      gtcgaaatt cgcgaaaat gttgaagag gccttgacg 
      aaacggcaa atgaacttc gagttgttg ctccaagtg 
      cagaaaaaa ctcaacact tataatgac ctggccaag 
      gggcgcaag ccccacttg gtgcaccgc tcggaaagc 
      gccttgtcc tcggcctac tctagggaa atttccacc 
      aattctaaa ctatgcaag attgtggaa aacctccag 
      tcggacttt cccaaccat gggcacgag ctggtccgc 
      atttgtaac acgctgaaa ctgcccgtt tatgttctt 
      ggttttgac tcattagta ctttacaaa aacatggag 
      ggtgtagga gcgtacgga ggcggtaaa gcaggaggt 
      gctttcgac ccgatttcc ttcgctcag cggccccaa 
      gtgctcctg agggccgtc tgctggctg ttctccatc 
      gtggtgttc ggctgtata tcgtcccaa ggctggcta 
      atcaacaaa aacggcaaa gaggagtgt ctgtataac 
      ggggacgcg aacgcgtgc aattacggg gttggtatc 
      agtgtgatt gccttcctg gccagcatg gggttcctg 
      gccggggaa tacctcttc gagcaaatg tcgtcggtc 
      aagacacgg aagcactac gttctgggc gacttggga 
      ttctcagca ttttgggcg ttcctgtat tttgtgggc 
      ttttggtac ctcgccagc caatggggt aagtccaag 
      acgccggaa aacggctac ggcgtgaac aacgtccag 
      gccgcaatc gccttttcc ttcttttcg attttctcc 
      tgggcgggt tgcgccttt ttggcgttc cagcgtttc 
      cggcagggc gctgatgca gcatttgcc accaactac 
      gaggccgat acgagtatg cctggctcg tatccgtct 
      tatcctggg ggccccgat tcagaccaa cactaccag 
      gagccccca ttttccgcc ggtcctaac caacgagga 
      ccgacggat ttccaagcg ccagcttat taa
    mRNA sequence
    • >TC034178 RA
      cttgaactt ttttaaaaa tcgataatt tcggtggcg 
      tacttcccc gcgagttta agcacgttg ctaggcgac 
      taatagcaa aacataaac aacttggca gtgacagtc 
      cagtgacag tgtctttat ttactgaca aataaaaat 
      ggacgagga gaataaagt gaattttga aagtgtaga 
      aaatgaggc aaaatcttc cccaacttt tacaccaca 
      ttcggcaaa atcgtccga aagagagga aactaatgt 
      cttgcaccc tgacaaccc ccttttgga aaaatttca 
      aaaagcgct caaggagca cttattgcg tcaaataaa 
      tcacctcaa aaacgacat tttcgaata cgattgtgg 
      gacgaaaaa aaacaatgc tagacgtga agaactggg 
      gattatggt ttacgaaca acaacaaat tttggaaaa 
      acaacaaaa aaccctaga tttgtacat ttctaaagt 
      gcaaactgc gacagcagc ctgcgaaga aatgcaaaa 
      cctagtcaa agaaaaaaa agaactgtt taaaaaaca 
      aagagacaa agtttacga agccgaggc taaagaact 
      cgaattgcg aaacgaaat cgaggcaac aaatcttct 
      agttcacca aatcagcga atgggagga gaacttgga 
      gtcagactt gacggtaaa aaagcgcgt ttttgagaa 
      aagccgcaa ggaaaagct caaactcct cgaagacaa 
      acgcactca agacgtcct catttacaa actaatgac 
      caggatctg gtttctcga acgcgaact ggacagaat 
      ggacgtgca aatgaaggt gaaagaggc ggaaaggga 
      ggatttggc ccaaactgt ggccctcgg gaacacaaa 
      catcgaagc aatggaagc ggaataccg ctgtctaat 
      gcactcctg gaactctgt cgttatcgc aatcggaaa 
      tcgcgataa agtcctctc gtgtctcaa caaagaact 
      gtccaagtc cgaggaaca cttcaaaag cgtggtttc 
      cgaaatcga acaagtcaa aaaactctc ccagaagga 
      aatgcgtcg caatgagga gttcacaat gttgaagaa 
      ccgcttcct ggccgatgt ccaggtgtg caaagcggc 
      tctggacgt cgaaatcga gaaaaaaac caccctgga 
      acaacgcat gttcgaaat gcaacgcat tattgaaca 
      aaccgaggc cgatattca acgcataac ggacgaagc 
      gcacgaaac acacaccat actttcggg gctgacgcg 
      cgaacacga acaactcga cacgcaaaa aggcgaact 
      cgaggagca aataatgcg agctttgga ggaacaagt 
      cactaataa taaaatgct gatgacgct acaacgcgc 
      attgacaca attcaaaat caaaaatcg ggaaattga 
      aatacaagt cgccacgat tgagaataa aatcgcatc 
      ggtgatgag cgaaatgga agcgcagaa atttgcaaa 
      ttttgaggg acaacggta tttgaagga gttggaaga 
      gcagttgaa ggagttggg aatggaggc ggaccgcta 
      ccaggacga gatgaaaca tattgagct taagattgc 
      aaagaaaca gcgggaagt tgatttgtt gaataacaa 
      gtatttgaa aatggtgga taagtcggg cgggacgtt 
      tgtctcgcc ccttgaaat gaaaataac aacactgga 
      gaaaaatat cgaggagat acaagagta caacaagaa 
      attgcaatt gtattggct gcgggaaca gtccaactt 
      gattaaaat ctcggaaga acggcagga acaaatcca 
      caataataa tctcatgtg taaacagat tttgatcct 
      ggagcagaa gaatttgag ggtttcgga cgagcttga 
      agcttgtcg caagttaca agataaagt cgttcgcag 
      tataaataa tctccacag tcaattgac cgtcatggg 
      tgataattt cgtcaagac taagggttt gaagataaa 
      tatggataa agataacga agtgttgcg tcaggatta 
      tatctacaa gcttaagga tgccgagct tgaatgcct 
      caaattgga ggatgagat tacgcaaat tgaggagga 
      taaggtgaa tttgagtaa tgaattgat tgcaatgaa 
      tcgtgaaac tctcgaatg ggagaagaa gtttttggg 
      ggcgaagga aacgaagaa tacgatgat tgaggaaag 
      ggggcagga aggggaggt tgggacgat gaaagcgga 
      gatacatcg catgaatgt gcgctatag ccaattgaa 
      gaaggtcca ggatgtctt ggtccaaga cttggagca 
      ctgcgtttc gcgaagaga cgcaatgat gacagctgc 
      ggaagcacg cgaaaaacg cgtaaaagg cggaatgga 
      aaaaactcg tattaattt caaccgcaa aatggacga 
      catgcgaaa caaatcgaa acaaatgga aaatcaaat 
      tgcggaaat ggccaaaaa aaccgaaca catggccaa 
      agaaattga gcaaatccg ggccgaaat cgccgaaat 
      gtcgcgcga gattgagac gacaaacga gcattgtgt 
      cgaaattcg cgaaaatgt tgaagaggc cttgacgaa 
      acggcaaat gaacttcga gttgttgct ccaagtgca 
      gaaaaaact caacactta taatgacct ggccaaggg 
      gcgcaagcc ccacttggt gcaccgctc ggaaagcgc 
      cttgtcctc ggcctactc tagggaaat ttccaccaa 
      ttctaaact atgcaagat tgtggaaaa cctccagtc 
      ggactttcc caaccatgg gcacgagct ggtccgcat 
      ttgtaacac gctgaaact gcccgttta tgttcttgg 
      ttttgactc attagtact ttacaaaaa catggaggg 
      tgtaggagc gtacggagg cggtaaagc aggaggtgc 
      tttcgaccc gatttcctt cgctcagcg gccccaagt 
      gctcctgag ggccgtctg ctggctgtt ctccatcgt 
      ggtgttcgg ctgtatatc gtcccaagg ctggctaat 
      caacaaaaa cggcaaaga ggagtgtct gtataacgg 
      ggacgcgaa cgcgtgcaa ttacggggt tggtatcag 
      tgtgattgc cttcctggc cagcatggg gttcctggc 
      cggggaata cctcttcga gcaaatgtc gtcggtcaa 
      gacacggaa gcactacgt tctgggcga cttgggatt 
      ctcagcatt ttgggcgtt cctgtattt tgtgggctt 
      ttggtacct cgccagcca atggggtaa gtccaagac 
      gccggaaaa cggctacgg cgtgaacaa cgtccaggc 
      cgcaatcgc cttttcctt cttttcgat tttctcctg 
      ggcgggttg cgccttttt ggcgttcca gcgtttccg 
      gcagggcgc tgatgcagc atttgccac caactacga 
      ggccgatac gagtatgcc tggctcgta tccgtctta 
      tcctggggg ccccgattc agaccaaca ctaccagga 
      gcccccatt ttccgccgg tcctaacca acgaggacc 
      gacggattt ccaagcgcc agcttatta actgggccc 
      ccaattctc ataatatag tgaacatag cgcagctgc 
      ctcatctct aaagcgagt cgaaaactc gccgacatt 
      ttcagtata taataaaag tcctttgta cttagaatc 
      gtacggaaa caaggtttt ggttttcag tattatttg 
      gatgaacct caaggagtg ttgttacgt tgcgttttt 
      gtaaagccg tgtacttat agtagctac ttatatcac 
      acacaaaac ttcaaaatg tgttgaatt ataaaaatg 
      ttacataat tatatagaa tgttcaatg tacagttat 
      ataataatt gtcgagttg cttgtagcg agttttgct 
      ttttttttc gaagcaatt ttaggcgtt tctgtgcta 
      gtagagtca gtgtgactc gaccgattg gcgcagtcg 
      gtaggatcg agtcgttgc acttttatt attttttta 
      accagtgcc attttttat gttttgata atgtgggat 
      caacttgtt tgcgaagcg attgttatt aacagtttt 
      ttgttgagg ttgagcgac aataaataa gcaattcac 
      gggcactaa ggtggcaaa aaaaacata ttgacagat 
      tttacatat ctggagcac aattttcta acaattggt 
      acgtctcat taaatactc aaaatttaa aatgctgct 
      tcgattatg atgaatttt attatctaa gtgggtgtc 
      gcatagtaa agttgttcc ttgtcacgg aaataggtc 
      attagcata atttaatta gcaataaaa gacaactgt 
      agatatgtt tcattatct cggttcggg atttggttt 
      cgttcaagt taacgtgaa ttgatataa agactatta 
      tttataatt atttttcta ttgaaatgt gatttttat 
      ttttatgga atattatat ttaggtgga cgaattgat 
      tcaatgtat taataagtt accaataaa tg