Status
Please wait ...
Gene information
General information
TC034178
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005401,TC005400 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13720990..13729997
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034178
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00847

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034178 RA
      MDEENKVNF ESVENEAKS SPTFTPHSA KSSEREETN 
VLHPDNPLL EKFQKALKE HLLRQINHL KNDIFEYDC 
GTKKNNARR EELGIMVYE QQQILEKQQ KTLDLYISK 
VQTATAACE EMQNLVKEK KELFKKQRD KVYEAEAKE 
LELRNEIEA TNLLVHQIS EWEENLESD LTVKKRVFE 
KSRKEKLKL LEDKRTQDV LIYKLMTRI WFLERELDR 
MDVQMKVKE AEREDLAQT VALGNTNIE AMEAEYRCL 
MHSWNSVVI AIGNRDKVL SCLNKELSK SEEHFKSVV 
SEIEQVKKL SQKEMRRNE EFTMLKNRF LADVQVCKA 
ALDVEIEKK TTLEQRMFE MQRIIEQTE ADIQRITDE 
AHETHTILS GLTREHEQL DTQKGELEE QIMRALEEQ 
VTNNKMLMT LQRALTQFK IKNREIEIQ VATIENKIA 
SVMSEMEAQ KFANFEGQR YLKELEEQL KELGMEADR 
YQDEMKHIE LKIAKKQRE VDLLNNKYL KMVDKSGGT 
FVSPLEMKI TTLEKNIEE IQEYNKKLQ LYWLREQSN 
LIKISEERQ EQIHNNNLM CKQILILEQ KNLRVSDEL 
EACRKLQDK VVRSINNLH SQLTVMGDN FVKTKGLKI 
NMDKDNEVL RQDYIYKLK DAELECLKL EDEITQIEE 
DKVNLSNEL IAMNRETLE WEKKFLGAK ETKNTMIEE 
RGQEGEVGT MKAEIHRMN VRYSQLKKV QDVLVQDLE 
HCVSRRDAM MTAAEAREK RVKGGMEKT RINFNRKMD 
DMRNKSKQM ENQIAEMAK KTEHMAKEI EQIRAEIAE 
MSREIETTN EHCVEIREN VEEALTKRQ MNFELLLQV 
QKKLNTYND LAKGRKPHL VHRSESALS SAYSREIST 
NSKLCKIVE NLQSDFPNH GHELVRICN TLKLPVYVL 
GFDSLVLYK NMEGVGAYG GGKAGGAFD PISFAQRPQ 
VLLRAVCWL FSIVVFGCI SSQGWLINK NGKEECLYN 
GDANACNYG VGISVIAFL ASMGFLAGE YLFEQMSSV 
KTRKHYVLG DLGFSAFWA FLYFVGFWY LASQWGKSK 
TPENGYGVN NVQAAIAFS FFSIFSWAG CAFLAFQRF 
RQGADAAFA TNYEADTSM PGSYPSYPG GPDSDQHYQ 
EPPFSAGPN QRGPTDFQA PAY
    CDS sequence
    • >TC034178 RA
      atggacgag gagaataaa gtgaatttt gaaagtgta 
gaaaatgag gcaaaatct tccccaact tttacacca 
cattcggca aaatcgtcc gaaagagag gaaactaat 
gtcttgcac cctgacaac ccccttttg gaaaaattt 
caaaaagcg ctcaaggag cacttattg cgtcaaata 
aatcacctc aaaaacgac attttcgaa tacgattgt 
gggacgaaa aaaaacaat gctagacgt gaagaactg 
gggattatg gtttacgaa caacaacaa attttggaa 
aaacaacaa aaaacccta gatttgtac atttctaaa 
gtgcaaact gcgacagca gcctgcgaa gaaatgcaa 
aacctagtc aaagaaaaa aaagaactg tttaaaaaa 
caaagagac aaagtttac gaagccgag gctaaagaa 
ctcgaattg cgaaacgaa atcgaggca acaaatctt 
ctagttcac caaatcagc gaatgggag gagaacttg 
gagtcagac ttgacggta aaaaagcgc gtttttgag 
aaaagccgc aaggaaaag ctcaaactc ctcgaagac 
aaacgcact caagacgtc ctcatttac aaactaatg 
accaggatc tggtttctc gaacgcgaa ctggacaga 
atggacgtg caaatgaag gtgaaagag gcggaaagg 
gaggatttg gcccaaact gtggccctc gggaacaca 
aacatcgaa gcaatggaa gcggaatac cgctgtcta 
atgcactcc tggaactct gtcgttatc gcaatcgga 
aatcgcgat aaagtcctc tcgtgtctc aacaaagaa 
ctgtccaag tccgaggaa cacttcaaa agcgtggtt 
tccgaaatc gaacaagtc aaaaaactc tcccagaag 
gaaatgcgt cgcaatgag gagttcaca atgttgaag 
aaccgcttc ctggccgat gtccaggtg tgcaaagcg 
gctctggac gtcgaaatc gagaaaaaa accaccctg 
gaacaacgc atgttcgaa atgcaacgc attattgaa 
caaaccgag gccgatatt caacgcata acggacgaa 
gcgcacgaa acacacacc atactttcg gggctgacg 
cgcgaacac gaacaactc gacacgcaa aaaggcgaa 
ctcgaggag caaataatg cgagctttg gaggaacaa 
gtcactaat aataaaatg ctgatgacg ctacaacgc 
gcattgaca caattcaaa atcaaaaat cgggaaatt 
gaaatacaa gtcgccacg attgagaat aaaatcgca 
tcggtgatg agcgaaatg gaagcgcag aaatttgca 
aattttgag ggacaacgg tatttgaag gagttggaa 
gagcagttg aaggagttg ggaatggag gcggaccgc 
taccaggac gagatgaaa catattgag cttaagatt 
gcaaagaaa cagcgggaa gttgatttg ttgaataac 
aagtatttg aaaatggtg gataagtcg ggcgggacg 
tttgtctcg ccccttgaa atgaaaata acaacactg 
gagaaaaat atcgaggag atacaagag tacaacaag 
aaattgcaa ttgtattgg ctgcgggaa cagtccaac 
ttgattaaa atctcggaa gaacggcag gaacaaatc 
cacaataat aatctcatg tgtaaacag attttgatc 
ctggagcag aagaatttg agggtttcg gacgagctt 
gaagcttgt cgcaagtta caagataaa gtcgttcgc 
agtataaat aatctccac agtcaattg accgtcatg 
ggtgataat ttcgtcaag actaagggt ttgaagata 
aatatggat aaagataac gaagtgttg cgtcaggat 
tatatctac aagcttaag gatgccgag cttgaatgc 
ctcaaattg gaggatgag attacgcaa attgaggag 
gataaggtg aatttgagt aatgaattg attgcaatg 
aatcgtgaa actctcgaa tgggagaag aagtttttg 
ggggcgaag gaaacgaag aatacgatg attgaggaa 
agggggcag gaaggggag gttgggacg atgaaagcg 
gagatacat cgcatgaat gtgcgctat agccaattg 
aagaaggtc caggatgtc ttggtccaa gacttggag 
cactgcgtt tcgcgaaga gacgcaatg atgacagct 
gcggaagca cgcgaaaaa cgcgtaaaa ggcggaatg 
gaaaaaact cgtattaat ttcaaccgc aaaatggac 
gacatgcga aacaaatcg aaacaaatg gaaaatcaa 
attgcggaa atggccaaa aaaaccgaa cacatggcc 
aaagaaatt gagcaaatc cgggccgaa atcgccgaa 
atgtcgcgc gagattgag acgacaaac gagcattgt 
gtcgaaatt cgcgaaaat gttgaagag gccttgacg 
aaacggcaa atgaacttc gagttgttg ctccaagtg 
cagaaaaaa ctcaacact tataatgac ctggccaag 
gggcgcaag ccccacttg gtgcaccgc tcggaaagc 
gccttgtcc tcggcctac tctagggaa atttccacc 
aattctaaa ctatgcaag attgtggaa aacctccag 
tcggacttt cccaaccat gggcacgag ctggtccgc 
atttgtaac acgctgaaa ctgcccgtt tatgttctt 
ggttttgac tcattagta ctttacaaa aacatggag 
ggtgtagga gcgtacgga ggcggtaaa gcaggaggt 
gctttcgac ccgatttcc ttcgctcag cggccccaa 
gtgctcctg agggccgtc tgctggctg ttctccatc 
gtggtgttc ggctgtata tcgtcccaa ggctggcta 
atcaacaaa aacggcaaa gaggagtgt ctgtataac 
ggggacgcg aacgcgtgc aattacggg gttggtatc 
agtgtgatt gccttcctg gccagcatg gggttcctg 
gccggggaa tacctcttc gagcaaatg tcgtcggtc 
aagacacgg aagcactac gttctgggc gacttggga 
ttctcagca ttttgggcg ttcctgtat tttgtgggc 
ttttggtac ctcgccagc caatggggt aagtccaag 
acgccggaa aacggctac ggcgtgaac aacgtccag 
gccgcaatc gccttttcc ttcttttcg attttctcc 
tgggcgggt tgcgccttt ttggcgttc cagcgtttc 
cggcagggc gctgatgca gcatttgcc accaactac 
gaggccgat acgagtatg cctggctcg tatccgtct 
tatcctggg ggccccgat tcagaccaa cactaccag 
gagccccca ttttccgcc ggtcctaac caacgagga 
ccgacggat ttccaagcg ccagcttat taa
    mRNA sequence
    • >TC034178 RA
      cttgaactt ttttaaaaa tcgataatt tcggtggcg 
tacttcccc gcgagttta agcacgttg ctaggcgac 
taatagcaa aacataaac aacttggca gtgacagtc 
cagtgacag tgtctttat ttactgaca aataaaaat 
ggacgagga gaataaagt gaattttga aagtgtaga 
aaatgaggc aaaatcttc cccaacttt tacaccaca 
ttcggcaaa atcgtccga aagagagga aactaatgt 
cttgcaccc tgacaaccc ccttttgga aaaatttca 
aaaagcgct caaggagca cttattgcg tcaaataaa 
tcacctcaa aaacgacat tttcgaata cgattgtgg 
gacgaaaaa aaacaatgc tagacgtga agaactggg 
gattatggt ttacgaaca acaacaaat tttggaaaa 
acaacaaaa aaccctaga tttgtacat ttctaaagt 
gcaaactgc gacagcagc ctgcgaaga aatgcaaaa 
cctagtcaa agaaaaaaa agaactgtt taaaaaaca 
aagagacaa agtttacga agccgaggc taaagaact 
cgaattgcg aaacgaaat cgaggcaac aaatcttct 
agttcacca aatcagcga atgggagga gaacttgga 
gtcagactt gacggtaaa aaagcgcgt ttttgagaa 
aagccgcaa ggaaaagct caaactcct cgaagacaa 
acgcactca agacgtcct catttacaa actaatgac 
caggatctg gtttctcga acgcgaact ggacagaat 
ggacgtgca aatgaaggt gaaagaggc ggaaaggga 
ggatttggc ccaaactgt ggccctcgg gaacacaaa 
catcgaagc aatggaagc ggaataccg ctgtctaat 
gcactcctg gaactctgt cgttatcgc aatcggaaa 
tcgcgataa agtcctctc gtgtctcaa caaagaact 
gtccaagtc cgaggaaca cttcaaaag cgtggtttc 
cgaaatcga acaagtcaa aaaactctc ccagaagga 
aatgcgtcg caatgagga gttcacaat gttgaagaa 
ccgcttcct ggccgatgt ccaggtgtg caaagcggc 
tctggacgt cgaaatcga gaaaaaaac caccctgga 
acaacgcat gttcgaaat gcaacgcat tattgaaca 
aaccgaggc cgatattca acgcataac ggacgaagc 
gcacgaaac acacaccat actttcggg gctgacgcg 
cgaacacga acaactcga cacgcaaaa aggcgaact 
cgaggagca aataatgcg agctttgga ggaacaagt 
cactaataa taaaatgct gatgacgct acaacgcgc 
attgacaca attcaaaat caaaaatcg ggaaattga 
aatacaagt cgccacgat tgagaataa aatcgcatc 
ggtgatgag cgaaatgga agcgcagaa atttgcaaa 
ttttgaggg acaacggta tttgaagga gttggaaga 
gcagttgaa ggagttggg aatggaggc ggaccgcta 
ccaggacga gatgaaaca tattgagct taagattgc 
aaagaaaca gcgggaagt tgatttgtt gaataacaa 
gtatttgaa aatggtgga taagtcggg cgggacgtt 
tgtctcgcc ccttgaaat gaaaataac aacactgga 
gaaaaatat cgaggagat acaagagta caacaagaa 
attgcaatt gtattggct gcgggaaca gtccaactt 
gattaaaat ctcggaaga acggcagga acaaatcca 
caataataa tctcatgtg taaacagat tttgatcct 
ggagcagaa gaatttgag ggtttcgga cgagcttga 
agcttgtcg caagttaca agataaagt cgttcgcag 
tataaataa tctccacag tcaattgac cgtcatggg 
tgataattt cgtcaagac taagggttt gaagataaa 
tatggataa agataacga agtgttgcg tcaggatta 
tatctacaa gcttaagga tgccgagct tgaatgcct 
caaattgga ggatgagat tacgcaaat tgaggagga 
taaggtgaa tttgagtaa tgaattgat tgcaatgaa 
tcgtgaaac tctcgaatg ggagaagaa gtttttggg 
ggcgaagga aacgaagaa tacgatgat tgaggaaag 
ggggcagga aggggaggt tgggacgat gaaagcgga 
gatacatcg catgaatgt gcgctatag ccaattgaa 
gaaggtcca ggatgtctt ggtccaaga cttggagca 
ctgcgtttc gcgaagaga cgcaatgat gacagctgc 
ggaagcacg cgaaaaacg cgtaaaagg cggaatgga 
aaaaactcg tattaattt caaccgcaa aatggacga 
catgcgaaa caaatcgaa acaaatgga aaatcaaat 
tgcggaaat ggccaaaaa aaccgaaca catggccaa 
agaaattga gcaaatccg ggccgaaat cgccgaaat 
gtcgcgcga gattgagac gacaaacga gcattgtgt 
cgaaattcg cgaaaatgt tgaagaggc cttgacgaa 
acggcaaat gaacttcga gttgttgct ccaagtgca 
gaaaaaact caacactta taatgacct ggccaaggg 
gcgcaagcc ccacttggt gcaccgctc ggaaagcgc 
cttgtcctc ggcctactc tagggaaat ttccaccaa 
ttctaaact atgcaagat tgtggaaaa cctccagtc 
ggactttcc caaccatgg gcacgagct ggtccgcat 
ttgtaacac gctgaaact gcccgttta tgttcttgg 
ttttgactc attagtact ttacaaaaa catggaggg 
tgtaggagc gtacggagg cggtaaagc aggaggtgc 
tttcgaccc gatttcctt cgctcagcg gccccaagt 
gctcctgag ggccgtctg ctggctgtt ctccatcgt 
ggtgttcgg ctgtatatc gtcccaagg ctggctaat 
caacaaaaa cggcaaaga ggagtgtct gtataacgg 
ggacgcgaa cgcgtgcaa ttacggggt tggtatcag 
tgtgattgc cttcctggc cagcatggg gttcctggc 
cggggaata cctcttcga gcaaatgtc gtcggtcaa 
gacacggaa gcactacgt tctgggcga cttgggatt 
ctcagcatt ttgggcgtt cctgtattt tgtgggctt 
ttggtacct cgccagcca atggggtaa gtccaagac 
gccggaaaa cggctacgg cgtgaacaa cgtccaggc 
cgcaatcgc cttttcctt cttttcgat tttctcctg 
ggcgggttg cgccttttt ggcgttcca gcgtttccg 
gcagggcgc tgatgcagc atttgccac caactacga 
ggccgatac gagtatgcc tggctcgta tccgtctta 
tcctggggg ccccgattc agaccaaca ctaccagga 
gcccccatt ttccgccgg tcctaacca acgaggacc 
gacggattt ccaagcgcc agcttatta actgggccc 
ccaattctc ataatatag tgaacatag cgcagctgc 
ctcatctct aaagcgagt cgaaaactc gccgacatt 
ttcagtata taataaaag tcctttgta cttagaatc 
gtacggaaa caaggtttt ggttttcag tattatttg 
gatgaacct caaggagtg ttgttacgt tgcgttttt 
gtaaagccg tgtacttat agtagctac ttatatcac 
acacaaaac ttcaaaatg tgttgaatt ataaaaatg 
ttacataat tatatagaa tgttcaatg tacagttat 
ataataatt gtcgagttg cttgtagcg agttttgct 
ttttttttc gaagcaatt ttaggcgtt tctgtgcta 
gtagagtca gtgtgactc gaccgattg gcgcagtcg 
gtaggatcg agtcgttgc acttttatt attttttta 
accagtgcc attttttat gttttgata atgtgggat 
caacttgtt tgcgaagcg attgttatt aacagtttt 
ttgttgagg ttgagcgac aataaataa gcaattcac 
gggcactaa ggtggcaaa aaaaacata ttgacagat 
tttacatat ctggagcac aattttcta acaattggt 
acgtctcat taaatactc aaaatttaa aatgctgct 
tcgattatg atgaatttt attatctaa gtgggtgtc 
gcatagtaa agttgttcc ttgtcacgg aaataggtc 
attagcata atttaatta gcaataaaa gacaactgt 
agatatgtt tcattatct cggttcggg atttggttt 
cgttcaagt taacgtgaa ttgatataa agactatta 
tttataatt atttttcta ttgaaatgt gatttttat 
ttttatgga atattatat ttaggtgga cgaattgat 
tcaatgtat taataagtt accaataaa tg

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY