Status
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Gene information
General information
TC005392 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13818900..13821567
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005392
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00845

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005392 RA
      MDNTAKTFV IPNELCRRA GVAASWLSV NEFGVFGHQ 
      KTPKVDKPS HFAPSDASV FQLRPDIIL FKPILRKLV 
      NEANGTFLT LQALVATEG DHKAELLKL SRQYRSIIR 
      ACLENLQDE VMRADGDYR EELQNYITI FYSVECIWH 
      LCEILFVDA VPGNIVLPH LLDWIRFHF PKYDRNAAS 
      MLAGDLGGL ESHPDYWET VIGSLLQGR VKVVRALLK 
      LHSEAESQP FRIVDQCLR AMPVYNMFS GTSIAEFNL 
      QWNHWVVDV QSKIDAKMF ISCYNLNLI MKLTVGNEI 
      AWGEIQQYC ETWYEMLAA WLFFTQPTV KSFELGQFA 
      KQCITKMSI GNRIKHLDR MLLAAMEFD ILQVIKEIQ 
      NMTDSGWFV SHLTDLLYH SGRLSDTTI ETIENFHPD 
      KLRESFLID YGSLLMGHK SLWQVGLSY LDHCPSDGP 
      EVTKLLLAR LPLNSEMRA EKIVREALN RNLTETAQS 
      ICKVQGMAC KNRGRLGHA LAWALKSQD GTFVSFLSN 
      KFLREYAES GDLNSIDLL DNLGSCILA SDRLMFLGK 
      YYEFQKLYQ ANEYKEAAN LLVSLLESK IIPNFFWSI 
      LLTDAIPLL ECEKMVFSS TDTYIIMQC LEEQEKTKD 
      LKDKLPILR LSATRNLAR ALVHEAQLK
    CDS sequence
    • >TC005392 RA
      atggacaat accgccaaa actttcgtt attccaaat 
      gagttatgc cgacgtgct ggagtggcg gcttcgtgg 
      ctttccgtc aacgaattc ggtgttttc ggccaccag 
      aaaacacca aaagttgat aaaccgagt cactttgcc 
      ccctcggac gcctctgtg tttcagctg cgccccgac 
      attattctc ttcaaacca attttacgc aaattggta 
      aatgaggcc aacgggacg tttctaacc cttcaagcc 
      cttgtagcc actgagggc gaccacaag gccgaatta 
      ttgaaactt agccggcag tacaggtcc ataataagg 
      gcctgtttg gagaatttg caagacgag gtgatgcga 
      gccgatggc gattatcgc gaagaattg caaaattac 
      attacgatt ttttactca gttgagtgt atttggcac 
      ttgtgtgaa atcttattt gttgatgcg gtcccaggc 
      aacattgtt ttgccccac ttactggac tggattcgg 
      tttcatttt ccgaaatat gaccgaaat gcggcaagt 
      atgttggct ggggatttg ggtggtttg gagtcacac 
      cctgactat tgggaaact gtcattggg tcactatta 
      caagggcgg gtcaaggtc gttagggcc ctacttaaa 
      ttacactca gaagctgag tcacaacct ttcagaatt 
      gtggatcag tgtctgaga gcaatgccg gtttataac 
      atgtttagt gggacttca atcgctgaa tttaatcta 
      caatggaat cattgggtc gtggatgtt caatcgaaa 
      attgatgcg aaaatgttt atttcttgt tataatctc 
      aatttgatt atgaagctt acggttggt aacgaaatt 
      gcatggggg gaaatacag cagtattgc gagacgtgg 
      tatgaaatg ttagccgcg tggttattt tttacccaa 
      cctactgtt aaatcgttt gagttggga cagtttgcc 
      aaacagtgt atcacgaaa atgtccata gggaataga 
      attaagcat ttggatagg atgttgttg gcagcaatg 
      gagtttgat attttacag gtgattaaa gaaatccaa 
      aatatgaca gacagtggc tggtttgtg agccactta 
      accgaccta ctttaccat tcaggccgt ctaagcgac 
      acaactatc gaaactatt gaaaatttc caccctgat 
      aaattgcgc gagtcattt ttaatcgat tatggctcg 
      cttttaatg ggccataag tcactatgg caggtcggt 
      ttaagctac ttggaccac tgtcccagc gatggccct 
      gaagtcaca aaattacta cttgcaaga ctgcctcta 
      aattccgaa atgcgggct gagaaaatt gtgagagag 
      gctctgaac cggaattta accgaaacg gcacaaagt 
      atttgtaaa gtgcaagga atggcttgt aaaaaccgg 
      gggcgtctg ggacacgcc ctcgcctgg gccctcaaa 
      tcacaagac ggcactttt gtgtcgttt ttatcgaac 
      aaatttctg cgagagtat gccgaaagt ggcgatttg 
      aacagtatt gatcttttg gataatctc ggatcgtgt 
      attttggca agcgatagg ttgatgttt ttaggcaaa 
      tattacgaa ttccagaag ttatatcaa gcgaacgag 
      tacaaagaa gccgccaat ttgctcgtt tcgttactt 
      gagtcgaaa ataatacca aactttttc tggagtatt 
      ctattaact gacgcaata ccgctactt gagtgcgaa 
      aaaatggtt ttttcctca actgatacc tacatcatt 
      atgcagtgt ttggaagag caggaaaaa accaaagac 
      ttgaaagac aaacttccg atcttgaga ctgtcagcg 
      acccggaat ttagcacga gctttggta cacgaagcc 
      caattaaaa tga
    mRNA sequence
    • >TC005392 RA
      acagatttc taaattacc gacccggct aaccgctct 
      cctcaaaca aaattcgaa aaaaatacg gtttttttg 
      aatgaaaag gcgcaaaat ttcgtgtta tttaacgct 
      tcccgaccc gtattaatg gacaatacc gccaaaact 
      ttcgttatt ccaaatgag ttatgccga cgtgctgga 
      gtggcggct tcgtggctt tccgtcaac gaattcggt 
      gttttcggc caccagaaa acaccaaaa gttgataaa 
      ccgagtcac tttgccccc tcggacgcc tctgtgttt 
      cagctgcgc cccgacatt attctcttc aaaccaatt 
      ttacgcaaa ttggtaaat gaggccaac gggacgttt 
      ctaaccctt caagccctt gtagccact gagggcgac 
      cacaaggcc gaattattg aaacttagc cggcagtac 
      aggtccata ataagggcc tgtttggag aatttgcaa 
      gacgaggtg atgcgagcc gatggcgat tatcgcgaa 
      gaattgcaa aattacatt acgattttt tactcagtt 
      gagtgtatt tggcacttg tgtgaaatc ttatttgtt 
      gatgcggtc ccaggcaac attgttttg ccccactta 
      ctggactgg attcggttt cattttccg aaatatgac 
      cgaaatgcg gcaagtatg ttggctggg gatttgggt 
      ggtttggag tcacaccct gactattgg gaaactgtc 
      attgggtca ctattacaa gggcgggtc aaggtcgtt 
      agggcccta cttaaatta cactcagaa gctgagtca 
      caacctttc agaattgtg gatcagtgt ctgagagca 
      atgccggtt tataacatg tttagtggg acttcaatc 
      gctgaattt aatctacaa tggaatcat tgggtcgtg 
      gatgttcaa tcgaaaatt gatgcgaaa atgtttatt 
      tcttgttat aatctcaat ttgattatg aagcttacg 
      gttggtaac gaaattgca tggggggaa atacagcag 
      tattgcgag acgtggtat gaaatgtta gccgcgtgg 
      ttatttttt acccaacct actgttaaa tcgtttgag 
      ttgggacag tttgccaaa cagtgtatc acgaaaatg 
      tccataggg aatagaatt aagcatttg gataggatg 
      ttgttggca gcaatggag tttgatatt ttacaggtg 
      attaaagaa atccaaaat atgacagac agtggctgg 
      tttgtgagc cacttaacc gacctactt taccattca 
      ggccgtcta agcgacaca actatcgaa actattgaa 
      aatttccac cctgataaa ttgcgcgag tcattttta 
      atcgattat ggctcgctt ttaatgggc cataagtca 
      ctatggcag gtcggttta agctacttg gaccactgt 
      cccagcgat ggccctgaa gtcacaaaa ttactactt 
      gcaagactg cctctaaat tccgaaatg cgggctgag 
      aaaattgtg agagaggct ctgaaccgg aatttaacc 
      gaaacggca caaagtatt tgtaaagtg caaggaatg 
      gcttgtaaa aaccggggg cgtctggga cacgccctc 
      gcctgggcc ctcaaatca caagacggc acttttgtg 
      tcgttttta tcgaacaaa tttctgcga gagtatgcc 
      gaaagtggc gatttgaac agtattgat cttttggat 
      aatctcgga tcgtgtatt ttggcaagc gataggttg 
      atgttttta ggcaaatat tacgaattc cagaagtta 
      tatcaagcg aacgagtac aaagaagcc gccaatttg 
      ctcgtttcg ttacttgag tcgaaaata ataccaaac 
      tttttctgg agtattcta ttaactgac gcaataccg 
      ctacttgag tgcgaaaaa atggttttt tcctcaact 
      gatacctac atcattatg cagtgtttg gaagagcag 
      gaaaaaacc aaagacttg aaagacaaa cttccgatc 
      ttgagactg tcagcgacc cggaattta gcacgagct 
      ttggtacac gaagcccaa ttaaaatga tgatttttt 
      taacaattt tttatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY