Status
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Gene information
General information
TC005385 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13855359..13857031
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005385
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00842

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005385 RA
      MLLSLFAVF LSVLIFFKF VSPCTYWKE KGVKHAQAW 
      PFIGSMGRV FFKKQPYVE FLTELYNKF PNERYVGFM 
      KFTQNHILI RDIELIGKI TGKEFNFFT DRTVSSRLR 
      KDKILSQSL ISLTGDHWR DMRATLSPA FTSNRTRNL 
      YTFIETCAR NFVNHYKAE VEIEVKEVF AKFANDVIA 
      SVAFSTELN SLKEPNDFY TNASQLKPD FFKQFITTS 
      DKNRQDLIS LLKDESLKF ETYKRAKGE GFAIGEEPQ 
      IDPLSRKIQ LTDEQILAQ ALLFFLTGF ETISTSASF 
      LAYELAVNL DVQKKLQRE IDKVVQITG DRIPYDALL 
      SMKYLDQVI SESLRKWPP KFETDRVCT RDYHIKAQK 
      GEKSLLIRK GMSVVIPIM AIHRDPDYY PNPNRFDPE 
      RFSDENRDK VVPGTYLPF GAGPRNCLG DDSVFLLTG 
      DWNCVSVGA RLALLEMKT VFGYLLAKF DIVQAKKTQ 
      MPIKLSAKC LDMEPEEGF WLGFRPRN
    CDS sequence
    • >TC005385 RA
      atgcttctc tcattgttt gctgtgttt ttaagtgtg 
      ttaattttt ttcaaattc gtaagtcct tgcacttac 
      tggaaggaa aaaggggtt aagcatgcc caagcttgg 
      ccgttcatt ggcagcatg ggccgggtt tttttcaaa 
      aaacaaccg tacgttgaa tttctaacc gaattatac 
      aacaaattt ccaaacgaa agatatgta ggtttcatg 
      aaattcact caaaaccac atcttgatc cgtgacatt 
      gaactaatc ggaaaaatc acagggaaa gagtttaat 
      ttttttaca gaccgaaca gtttcgtct cgactcaga 
      aaagataaa attttgtcc caaagttta atttcgctc 
      actggtgat cattggaga gacatgagg gccacccta 
      agcccagct ttcaccagc aataggacc agaaacttg 
      tacactttt attgagacg tgtgcccga aatttcgtc 
      aatcactac aaagccgag gttgaaatt gaagtcaaa 
      gaagtgttt gcaaaattt gccaacgac gtcattgct 
      tcagtcgct ttcagtacc gaactgaac tcactcaaa 
      gaaccgaac gatttttac acaaacgcc tcacaactc 
      aagccagac ttcttcaaa caatttatc accacaagt 
      gataaaaat cgccaagac ttgatcagt ttactaaag 
      gatgaaagt ttaaaattc gagacgtac aaaagggca 
      aagggtgaa ggcttcgcc attggggag gaaccacaa 
      atcgatccc ttgtccaga aaaatccaa ctcacagat 
      gagcaaatt ctggcccaa gccttactt tttttcctg 
      actggtttc gagacaatt tcaacgtcg gccagcttc 
      ctggcttac gaacttgca gtcaatttg gacgtccag 
      aaaaaacta caacgcgag atcgacaaa gtggtgcaa 
      ataaccggc gatagaatc ccttatgat gctttactt 
      tcaatgaaa tatctggac caagtcatt tcagaaagt 
      ttacgcaag tggccgcca aaattcgaa actgaccgg 
      gtttgcacc cgcgattac cacatcaag gcccagaag 
      ggcgaaaaa tccctcttg atacgaaaa gggatgtct 
      gtagttatc ccaataatg gcaatacat cgggaccct 
      gattactac cctaaccct aatcgcttc gatcctgag 
      cgttttagt gacgaaaat agggacaag gtggttcca 
      gggacgtat ctaccgttt ggagcgggg ccaaggaat 
      tgtcttggt gatgacagt gtgttttta ttgacaggg 
      gattggaat tgtgtgtcc gtaggtgcg agattagca 
      ctgttggaa atgaaaaca gtgttcggt tatttgctg 
      gcaaagttt gacattgtt caggctaag aaaactcaa 
      atgccgatt aaacttagc gccaagtgt ttggatatg 
      gagccggag gaagggttc tggctcggg ttcaggccc 
      agaaactaa
    mRNA sequence
    • >TC005385 RA
      gttgcaaca tttaaccga aacacaatg cttctctca 
      ttgtttgct gtgttttta agtgtgtta atttttttc 
      aaattcgta agtccttgc acttactgg aaggaaaaa 
      ggggttaag catgcccaa gcttggccg ttcattggc 
      agcatgggc cgggttttt ttcaaaaaa caaccgtac 
      gttgaattt ctaaccgaa ttatacaac aaatttcca 
      aacgaaaga tatgtaggt ttcatgaaa ttcactcaa 
      aaccacatc ttgatccgt gacattgaa ctaatcgga 
      aaaatcaca gggaaagag tttaatttt tttacagac 
      cgaacagtt tcgtctcga ctcagaaaa gataaaatt 
      ttgtcccaa agtttaatt tcgctcact ggtgatcat 
      tggagagac atgagggcc accctaagc ccagctttc 
      accagcaat aggaccaga aacttgtac acttttatt 
      gagacgtgt gcccgaaat ttcgtcaat cactacaaa 
      gccgaggtt gaaattgaa gtcaaagaa gtgtttgca 
      aaatttgcc aacgacgtc attgcttca gtcgctttc 
      agtaccgaa ctgaactca ctcaaagaa ccgaacgat 
      ttttacaca aacgcctca caactcaag ccagacttc 
      ttcaaacaa tttatcacc acaagtgat aaaaatcgc 
      caagacttg atcagttta ctaaaggat gaaagttta 
      aaattcgag acgtacaaa agggcaaag ggtgaaggc 
      ttcgccatt ggggaggaa ccacaaatc gatcccttg 
      tccagaaaa atccaactc acagatgag caaattctg 
      gcccaagcc ttacttttt ttcctgact ggtttcgag 
      acaatttca acgtcggcc agcttcctg gcttacgaa 
      cttgcagtc aatttggac gtccagaaa aaactacaa 
      cgcgagatc gacaaagtg gtgcaaata accggcgat 
      agaatccct tatgatgct ttactttca atgaaatat 
      ctggaccaa gtcatttca gaaagttta cgcaagtgg 
      ccgccaaaa ttcgaaact gaccgggtt tgcacccgc 
      gattaccac atcaaggcc cagaagggc gaaaaatcc 
      ctcttgata cgaaaaggg atgtctgta gttatccca 
      ataatggca atacatcgg gaccctgat tactaccct 
      aaccctaat cgcttcgat cctgagcgt tttagtgac 
      gaaaatagg gacaaggtg gttccaggg acgtatcta 
      ccgtttgga gcggggcca aggaattgt cttggtgat 
      gacagtgtg tttttattg acaggggat tggaattgt 
      gtgtccgta ggtgcgaga ttagcactg ttggaaatg 
      aaaacagtg ttcggttat ttgctggca aagtttgac 
      attgttcag gctaagaaa actcaaatg ccgattaaa 
      cttagcgcc aagtgtttg gatatggag ccggaggaa 
      gggttctgg ctcgggttc aggcccaga aactaattg 
      aaatcaagc aaaatcatt gtgttattt attttccct 
      aactcgcaa tttttcgaa ataaatctg ttttaaatg 
      g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY