GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00841 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
ovary without detectable defects - Number of animals showing the phenotype: 4
- >TC005384 RA
MWWFFVLLL GAPIVFFIF LYRTQKHWE RRGLKQKNM GVTIPDNWA ETKYPITFP DYVQKFYEM FPNRRYVGY YTISNPSLF IRDLDLIKQ IIVKDFEYF TDHQHFIWE ENDPLWSQN LFSLKGKKW REMRTTLSP TFTSSKIKY MFTLVQKNG EQFARYFLD KNENFMVFE MKDIFTRFT NDVIASSAF GVECDSLRE PQNEFYLMS KAVTDFTGL WKIVAGTFY FLMPSVAKF LNISLFPKK GSKFFSNLV KSNIKSRLQ HGIVRPDMI NIMLEARKH GLKHEETQP SQDTGFATI EESELGKSG KVKKEITDD DITSQALVF IFAGFETVS SMMCFLSYE LALNPDIQK RLIQEIDQT LDACGGSIT YESLMGMKY LDMVLTESL RKWPISAGL DRVCTKEYV IEPKLPGEN PVRVDKGTT ILVPVYALL RDPKYFPNP EKFDPERFS DENKGKIVP YTFLPFGAG PRSCIGTRF AIMETKVLF FSILRHFEI VRVEKTAVP IRYIPQQIT LSSADGFWL GFKRRVKV
- >TC005384 RA
atgtggtgg ttttttgtg cttttgcta ggcgccccc attgtgttc tttattttt ttatatcga actcagaag cactgggag aggagagga ctgaagcag aagaatatg ggcgtcacg ataccagat aactgggct gagaccaaa tatcccatc acttttcca gactacgta caaaagttc tacgaaatg tttcccaac agaagatac gtgggctat tacaccatc tcaaacccc agccttttt atccgcgac ctcgaccta atcaaacaa atcatagtc aaagacttt gaatatttc accgaccac cagcacttc atctgggag gagaacgac cctctctgg agccagaac ctcttctct ttgaagggc aaaaagtgg cgcgaaatg cgcacaacc cttagccct accttcacc agcagcaaa atcaaatac atgttcact ctagtccag aaaaacggc gaacagttc gctcgctat ttcctcgac aaaaacgag aactttatg gttttcgaa atgaaggac atcttcaca cgtttcacc aacgacgtc attgccagc agtgccttt ggggtcgaa tgcgactcg ctacgagag ccccaaaac gagttctat ctgatgagc aaagctgtg actgatttt acgggactt tggaaaatt gtagctggg actttttat tttttgatg ccttcagtg gcaaaattc ctgaacata agtctgttc ccgaagaaa ggcagcaag ttttttagc aacttggtc aaaagtaac atcaaaagt cgcctccag cacggtata gtccgccct gacatgatc aacataatg ctagaagca cgcaaacat ggcttaaaa cacgaggaa acccaaccc agtcaagac accggtttt gcaacaatc gaggaatca gaacttgga aaaagtggc aaagtcaag aaggagatc actgacgat gacataact tcccaagcc cttgttttc attttcgcc ggttttgaa accgtctcc tcgatgatg tgcttcctg agctacgaa ctcgccctt aatcccgac atccagaag cgcctgatc caggaaatc gaccaaact ctggacgcc tgcggcggt tccataaca tacgagagc ctaatgggc atgaagtac ctggacatg gttttgacc gagagcctc cgaaagtgg ccgatttcc gccggtttg gaccgcgtt tgcacgaag gagtacgtc atcgagccg aagctgccc ggggaaaac ccggtgagg gttgataag ggtactacg attctggtg ccggtttat gcccttcta agggatccc aagtacttt ccgaacccg gaaaagttt gatccggaa cggttcagt gatgaaaat aagggtaaa atagtgccg tatacgttt ttgcccttt ggggctggg cccaggagt tgcattggg acgaggttt gcgattatg gagaccaag gttttgttt ttctcgatt ttgaggcac tttgagatt gtgagggtg gagaaaact gcggttccg attaggtat ataccgcag caaatcacg ctgtcgtcg gctgatggg ttctggtta gggttcaag cgaagggtt aaggtttaa
- >TC005384 RA
tgctgcata taaccaaac tgcactatt aacacgtcc agtagaaac caagtcgtg agaaaaaat gtggtggtt ttttgtgct tttgctagg cgcccccat tgtgttctt tattttttt atatcgaac tcagaagca ctgggagag gagaggact gaagcagaa gaatatggg cgtcacgat accagataa ctgggctga gaccaaata tcccatcac ttttccaga ctacgtaca aaagttcta cgaaatgtt tcccaacag aagatacgt gggctatta caccatctc aaaccccag cctttttat ccgcgacct cgacctaat caaacaaat catagtcaa agactttga atatttcac cgaccacca gcacttcat ctgggagga gaacgaccc tctctggag ccagaacct cttctcttt gaagggcaa aaagtggcg cgaaatgcg cacaaccct tagccctac cttcaccag cagcaaaat caaatacat gttcactct agtccagaa aaacggcga acagttcgc tcgctattt cctcgacaa aaacgagaa ctttatggt tttcgaaat gaaggacat cttcacacg tttcaccaa cgacgtcat tgccagcag tgcctttgg ggtcgaatg cgactcgct acgagagcc ccaaaacga gttctatct gatgagcaa agctgtgac tgattttac gggactttg gaaaattgt agctgggac tttttattt tttgatgcc ttcagtggc aaaattcct gaacataag tctgttccc gaagaaagg cagcaagtt ttttagcaa cttggtcaa aagtaacat caaaagtcg cctccagca cggtatagt ccgccctga catgatcaa cataatgct agaagcacg caaacatgg cttaaaaca cgaggaaac ccaacccag tcaagacac cggttttgc aacaatcga ggaatcaga acttggaaa aagtggcaa agtcaagaa ggagatcac tgacgatga cataacttc ccaagccct tgttttcat tttcgccgg ttttgaaac cgtctcctc gatgatgtg cttcctgag ctacgaact cgcccttaa tcccgacat ccagaagcg cctgatcca ggaaatcga ccaaactct ggacgcctg cggcggttc cataacata cgagagcct aatgggcat gaagtacct ggacatggt tttgaccga gagcctccg aaagtggcc gatttccgc cggtttgga ccgcgtttg cacgaagga gtacgtcat cgagccgaa gctgcccgg ggaaaaccc ggtgagggt tgataaggg tactacgat tctggtgcc ggtttatgc ccttctaag ggatcccaa gtactttcc gaacccgga aaagtttga tccggaacg gttcagtga tgaaaataa gggtaaaat agtgccgta tacgttttt gccctttgg ggctgggcc caggagttg cattgggac gaggtttgc gattatgga gaccaaggt tttgttttt ctcgatttt gaggcactt tgagattgt gagggtgga gaaaactgc ggttccgat taggtatat accgcagca aatcacgct gtcgtcggc tgatgggtt ctggttagg gttcaagcg aagggttaa ggtttaagg ggaaatcaa taaagttgg ttcggaatt
