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Gene information
General information
TC005384 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13858347..13861961
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC005384
    iBeetle screen iB_00841

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number no/almost no eggs -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005384 RA
      MWWFFVLLL GAPIVFFIF LYRTQKHWE RRGLKQKNM 
GVTIPDNWA ETKYPITFP DYVQKFYEM FPNRRYVGY 
YTISNPSLF IRDLDLIKQ IIVKDFEYF TDHQHFIWE 
ENDPLWSQN LFSLKGKKW REMRTTLSP TFTSSKIKY 
MFTLVQKNG EQFARYFLD KNENFMVFE MKDIFTRFT 
NDVIASSAF GVECDSLRE PQNEFYLMS KAVTDFTGL 
WKIVAGTFY FLMPSVAKF LNISLFPKK GSKFFSNLV 
KSNIKSRLQ HGIVRPDMI NIMLEARKH GLKHEETQP 
SQDTGFATI EESELGKSG KVKKEITDD DITSQALVF 
IFAGFETVS SMMCFLSYE LALNPDIQK RLIQEIDQT 
LDACGGSIT YESLMGMKY LDMVLTESL RKWPISAGL 
DRVCTKEYV IEPKLPGEN PVRVDKGTT ILVPVYALL 
RDPKYFPNP EKFDPERFS DENKGKIVP YTFLPFGAG 
PRSCIGTRF AIMETKVLF FSILRHFEI VRVEKTAVP 
IRYIPQQIT LSSADGFWL GFKRRVKV
    CDS sequence
    • >TC005384 RA
      atgtggtgg ttttttgtg cttttgcta ggcgccccc 
attgtgttc tttattttt ttatatcga actcagaag 
cactgggag aggagagga ctgaagcag aagaatatg 
ggcgtcacg ataccagat aactgggct gagaccaaa 
tatcccatc acttttcca gactacgta caaaagttc 
tacgaaatg tttcccaac agaagatac gtgggctat 
tacaccatc tcaaacccc agccttttt atccgcgac 
ctcgaccta atcaaacaa atcatagtc aaagacttt 
gaatatttc accgaccac cagcacttc atctgggag 
gagaacgac cctctctgg agccagaac ctcttctct 
ttgaagggc aaaaagtgg cgcgaaatg cgcacaacc 
cttagccct accttcacc agcagcaaa atcaaatac 
atgttcact ctagtccag aaaaacggc gaacagttc 
gctcgctat ttcctcgac aaaaacgag aactttatg 
gttttcgaa atgaaggac atcttcaca cgtttcacc 
aacgacgtc attgccagc agtgccttt ggggtcgaa 
tgcgactcg ctacgagag ccccaaaac gagttctat 
ctgatgagc aaagctgtg actgatttt acgggactt 
tggaaaatt gtagctggg actttttat tttttgatg 
ccttcagtg gcaaaattc ctgaacata agtctgttc 
ccgaagaaa ggcagcaag ttttttagc aacttggtc 
aaaagtaac atcaaaagt cgcctccag cacggtata 
gtccgccct gacatgatc aacataatg ctagaagca 
cgcaaacat ggcttaaaa cacgaggaa acccaaccc 
agtcaagac accggtttt gcaacaatc gaggaatca 
gaacttgga aaaagtggc aaagtcaag aaggagatc 
actgacgat gacataact tcccaagcc cttgttttc 
attttcgcc ggttttgaa accgtctcc tcgatgatg 
tgcttcctg agctacgaa ctcgccctt aatcccgac 
atccagaag cgcctgatc caggaaatc gaccaaact 
ctggacgcc tgcggcggt tccataaca tacgagagc 
ctaatgggc atgaagtac ctggacatg gttttgacc 
gagagcctc cgaaagtgg ccgatttcc gccggtttg 
gaccgcgtt tgcacgaag gagtacgtc atcgagccg 
aagctgccc ggggaaaac ccggtgagg gttgataag 
ggtactacg attctggtg ccggtttat gcccttcta 
agggatccc aagtacttt ccgaacccg gaaaagttt 
gatccggaa cggttcagt gatgaaaat aagggtaaa 
atagtgccg tatacgttt ttgcccttt ggggctggg 
cccaggagt tgcattggg acgaggttt gcgattatg 
gagaccaag gttttgttt ttctcgatt ttgaggcac 
tttgagatt gtgagggtg gagaaaact gcggttccg 
attaggtat ataccgcag caaatcacg ctgtcgtcg 
gctgatggg ttctggtta gggttcaag cgaagggtt 
aaggtttaa
    mRNA sequence
    • >TC005384 RA
      tgctgcata taaccaaac tgcactatt aacacgtcc 
agtagaaac caagtcgtg agaaaaaat gtggtggtt 
ttttgtgct tttgctagg cgcccccat tgtgttctt 
tattttttt atatcgaac tcagaagca ctgggagag 
gagaggact gaagcagaa gaatatggg cgtcacgat 
accagataa ctgggctga gaccaaata tcccatcac 
ttttccaga ctacgtaca aaagttcta cgaaatgtt 
tcccaacag aagatacgt gggctatta caccatctc 
aaaccccag cctttttat ccgcgacct cgacctaat 
caaacaaat catagtcaa agactttga atatttcac 
cgaccacca gcacttcat ctgggagga gaacgaccc 
tctctggag ccagaacct cttctcttt gaagggcaa 
aaagtggcg cgaaatgcg cacaaccct tagccctac 
cttcaccag cagcaaaat caaatacat gttcactct 
agtccagaa aaacggcga acagttcgc tcgctattt 
cctcgacaa aaacgagaa ctttatggt tttcgaaat 
gaaggacat cttcacacg tttcaccaa cgacgtcat 
tgccagcag tgcctttgg ggtcgaatg cgactcgct 
acgagagcc ccaaaacga gttctatct gatgagcaa 
agctgtgac tgattttac gggactttg gaaaattgt 
agctgggac tttttattt tttgatgcc ttcagtggc 
aaaattcct gaacataag tctgttccc gaagaaagg 
cagcaagtt ttttagcaa cttggtcaa aagtaacat 
caaaagtcg cctccagca cggtatagt ccgccctga 
catgatcaa cataatgct agaagcacg caaacatgg 
cttaaaaca cgaggaaac ccaacccag tcaagacac 
cggttttgc aacaatcga ggaatcaga acttggaaa 
aagtggcaa agtcaagaa ggagatcac tgacgatga 
cataacttc ccaagccct tgttttcat tttcgccgg 
ttttgaaac cgtctcctc gatgatgtg cttcctgag 
ctacgaact cgcccttaa tcccgacat ccagaagcg 
cctgatcca ggaaatcga ccaaactct ggacgcctg 
cggcggttc cataacata cgagagcct aatgggcat 
gaagtacct ggacatggt tttgaccga gagcctccg 
aaagtggcc gatttccgc cggtttgga ccgcgtttg 
cacgaagga gtacgtcat cgagccgaa gctgcccgg 
ggaaaaccc ggtgagggt tgataaggg tactacgat 
tctggtgcc ggtttatgc ccttctaag ggatcccaa 
gtactttcc gaacccgga aaagtttga tccggaacg 
gttcagtga tgaaaataa gggtaaaat agtgccgta 
tacgttttt gccctttgg ggctgggcc caggagttg 
cattgggac gaggtttgc gattatgga gaccaaggt 
tttgttttt ctcgatttt gaggcactt tgagattgt 
gagggtgga gaaaactgc ggttccgat taggtatat 
accgcagca aatcacgct gtcgtcggc tgatgggtt 
ctggttagg gttcaagcg aagggttaa ggtttaagg 
ggaaatcaa taaagttgg ttcggaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY