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Gene information
General information
TC005384 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13858347..13861961
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005384
    iBeetle screen iB_00841

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number no/almost no eggs -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005384 RA
      MWWFFVLLL GAPIVFFIF LYRTQKHWE RRGLKQKNM 
      GVTIPDNWA ETKYPITFP DYVQKFYEM FPNRRYVGY 
      YTISNPSLF IRDLDLIKQ IIVKDFEYF TDHQHFIWE 
      ENDPLWSQN LFSLKGKKW REMRTTLSP TFTSSKIKY 
      MFTLVQKNG EQFARYFLD KNENFMVFE MKDIFTRFT 
      NDVIASSAF GVECDSLRE PQNEFYLMS KAVTDFTGL 
      WKIVAGTFY FLMPSVAKF LNISLFPKK GSKFFSNLV 
      KSNIKSRLQ HGIVRPDMI NIMLEARKH GLKHEETQP 
      SQDTGFATI EESELGKSG KVKKEITDD DITSQALVF 
      IFAGFETVS SMMCFLSYE LALNPDIQK RLIQEIDQT 
      LDACGGSIT YESLMGMKY LDMVLTESL RKWPISAGL 
      DRVCTKEYV IEPKLPGEN PVRVDKGTT ILVPVYALL 
      RDPKYFPNP EKFDPERFS DENKGKIVP YTFLPFGAG 
      PRSCIGTRF AIMETKVLF FSILRHFEI VRVEKTAVP 
      IRYIPQQIT LSSADGFWL GFKRRVKV
    CDS sequence
    • >TC005384 RA
      atgtggtgg ttttttgtg cttttgcta ggcgccccc 
      attgtgttc tttattttt ttatatcga actcagaag 
      cactgggag aggagagga ctgaagcag aagaatatg 
      ggcgtcacg ataccagat aactgggct gagaccaaa 
      tatcccatc acttttcca gactacgta caaaagttc 
      tacgaaatg tttcccaac agaagatac gtgggctat 
      tacaccatc tcaaacccc agccttttt atccgcgac 
      ctcgaccta atcaaacaa atcatagtc aaagacttt 
      gaatatttc accgaccac cagcacttc atctgggag 
      gagaacgac cctctctgg agccagaac ctcttctct 
      ttgaagggc aaaaagtgg cgcgaaatg cgcacaacc 
      cttagccct accttcacc agcagcaaa atcaaatac 
      atgttcact ctagtccag aaaaacggc gaacagttc 
      gctcgctat ttcctcgac aaaaacgag aactttatg 
      gttttcgaa atgaaggac atcttcaca cgtttcacc 
      aacgacgtc attgccagc agtgccttt ggggtcgaa 
      tgcgactcg ctacgagag ccccaaaac gagttctat 
      ctgatgagc aaagctgtg actgatttt acgggactt 
      tggaaaatt gtagctggg actttttat tttttgatg 
      ccttcagtg gcaaaattc ctgaacata agtctgttc 
      ccgaagaaa ggcagcaag ttttttagc aacttggtc 
      aaaagtaac atcaaaagt cgcctccag cacggtata 
      gtccgccct gacatgatc aacataatg ctagaagca 
      cgcaaacat ggcttaaaa cacgaggaa acccaaccc 
      agtcaagac accggtttt gcaacaatc gaggaatca 
      gaacttgga aaaagtggc aaagtcaag aaggagatc 
      actgacgat gacataact tcccaagcc cttgttttc 
      attttcgcc ggttttgaa accgtctcc tcgatgatg 
      tgcttcctg agctacgaa ctcgccctt aatcccgac 
      atccagaag cgcctgatc caggaaatc gaccaaact 
      ctggacgcc tgcggcggt tccataaca tacgagagc 
      ctaatgggc atgaagtac ctggacatg gttttgacc 
      gagagcctc cgaaagtgg ccgatttcc gccggtttg 
      gaccgcgtt tgcacgaag gagtacgtc atcgagccg 
      aagctgccc ggggaaaac ccggtgagg gttgataag 
      ggtactacg attctggtg ccggtttat gcccttcta 
      agggatccc aagtacttt ccgaacccg gaaaagttt 
      gatccggaa cggttcagt gatgaaaat aagggtaaa 
      atagtgccg tatacgttt ttgcccttt ggggctggg 
      cccaggagt tgcattggg acgaggttt gcgattatg 
      gagaccaag gttttgttt ttctcgatt ttgaggcac 
      tttgagatt gtgagggtg gagaaaact gcggttccg 
      attaggtat ataccgcag caaatcacg ctgtcgtcg 
      gctgatggg ttctggtta gggttcaag cgaagggtt 
      aaggtttaa
    mRNA sequence
    • >TC005384 RA
      tgctgcata taaccaaac tgcactatt aacacgtcc 
      agtagaaac caagtcgtg agaaaaaat gtggtggtt 
      ttttgtgct tttgctagg cgcccccat tgtgttctt 
      tattttttt atatcgaac tcagaagca ctgggagag 
      gagaggact gaagcagaa gaatatggg cgtcacgat 
      accagataa ctgggctga gaccaaata tcccatcac 
      ttttccaga ctacgtaca aaagttcta cgaaatgtt 
      tcccaacag aagatacgt gggctatta caccatctc 
      aaaccccag cctttttat ccgcgacct cgacctaat 
      caaacaaat catagtcaa agactttga atatttcac 
      cgaccacca gcacttcat ctgggagga gaacgaccc 
      tctctggag ccagaacct cttctcttt gaagggcaa 
      aaagtggcg cgaaatgcg cacaaccct tagccctac 
      cttcaccag cagcaaaat caaatacat gttcactct 
      agtccagaa aaacggcga acagttcgc tcgctattt 
      cctcgacaa aaacgagaa ctttatggt tttcgaaat 
      gaaggacat cttcacacg tttcaccaa cgacgtcat 
      tgccagcag tgcctttgg ggtcgaatg cgactcgct 
      acgagagcc ccaaaacga gttctatct gatgagcaa 
      agctgtgac tgattttac gggactttg gaaaattgt 
      agctgggac tttttattt tttgatgcc ttcagtggc 
      aaaattcct gaacataag tctgttccc gaagaaagg 
      cagcaagtt ttttagcaa cttggtcaa aagtaacat 
      caaaagtcg cctccagca cggtatagt ccgccctga 
      catgatcaa cataatgct agaagcacg caaacatgg 
      cttaaaaca cgaggaaac ccaacccag tcaagacac 
      cggttttgc aacaatcga ggaatcaga acttggaaa 
      aagtggcaa agtcaagaa ggagatcac tgacgatga 
      cataacttc ccaagccct tgttttcat tttcgccgg 
      ttttgaaac cgtctcctc gatgatgtg cttcctgag 
      ctacgaact cgcccttaa tcccgacat ccagaagcg 
      cctgatcca ggaaatcga ccaaactct ggacgcctg 
      cggcggttc cataacata cgagagcct aatgggcat 
      gaagtacct ggacatggt tttgaccga gagcctccg 
      aaagtggcc gatttccgc cggtttgga ccgcgtttg 
      cacgaagga gtacgtcat cgagccgaa gctgcccgg 
      ggaaaaccc ggtgagggt tgataaggg tactacgat 
      tctggtgcc ggtttatgc ccttctaag ggatcccaa 
      gtactttcc gaacccgga aaagtttga tccggaacg 
      gttcagtga tgaaaataa gggtaaaat agtgccgta 
      tacgttttt gccctttgg ggctgggcc caggagttg 
      cattgggac gaggtttgc gattatgga gaccaaggt 
      tttgttttt ctcgatttt gaggcactt tgagattgt 
      gagggtgga gaaaactgc ggttccgat taggtatat 
      accgcagca aatcacgct gtcgtcggc tgatgggtt 
      ctggttagg gttcaagcg aagggttaa ggtttaagg 
      ggaaatcaa taaagttgg ttcggaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY