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Gene information
General information
TC005375 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid58: 761660..764698
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005375
    iBeetle screen iB_00838
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    procephalic appendages partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    gnathal appendages partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax segment sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005375 RA
      MRFIVVAIL GCCALALAF PERKITNKQ FLERQRDIL 
      RLFRHINQP SYYKDHQEI AQSFHLHDH YDHYTKPEI 
      AKHYHQIYE YGLLPRGEV FSVFYEEHL QQAIALYKL 
      FYYAKDYDT FYRTAVWAR QHVNEGVFL YSFSVAIVH 
      REDTYGIVL PPIYEIYPH YFYNNEVIQ EAYRYKQQY 
      YNQEHGYTI NANYSGFYL NLHPEQSLS YFTEDVGVN 
      SFYYYYNIY YPHWLGGED FDFAHDRRG EQYYYVYQQ 
      ILARYYLER LSNDFGEIP FFNYEVPFE NGYYPLLQY 
      PNGLFFPQR PNYAKLYEY FYNYGQKYG NNRYAYSYT 
      FVQDYERRI RDAIDRGYV FSHDGQRIN LFSEDGVNI 
      LGNLIESNP DSPDRHFYG ALHVYARHL LGYSSQPLD 
      KYHVAPSAL QHYETSLRD PAFYQFYKR IVLYFQKYK 
      SYLPSYTEH DLHFQGVEV KSVEFDRLV TYFDHFYTD 
      ISNAVYVTP QEYDSEKVQ VRVRQYRLN HKPFTYRVH 
      VSSDKEQQA VVRIYLGPK YDEYGRYIN ISHNRLNFV 
      EVDHFKYQL KVGENVIER NSHQNYFYQ NDRTSYRQL 
      YKQVLGALN GNGEFNVNA NEAYFGFPR RFLLPKGNY 
      GGQEFQFFV IVSPYVPYK YHQEGYDAS KYYYPRVGS 
      GAHHIDNYA FGYPFDRPI HYDQIFHNV PNAYFYTTK 
      IYHRHADDI NASTSAHQ
    CDS sequence
    • >TC005375 RA
      atgaggttc attgtagtg gcgatcctt ggctgttgt 
      gccctggct ttggctttc cccgaacgt aaaataacc 
      aataaacaa ttcctcgag agacaaaga gacatcctt 
      cgcctgttc cgacacata aaccagcca agttactac 
      aaagaccac caggagata gcccagagt ttccacctc 
      catgaccac tacgaccac tacactaaa cctgaaatt 
      gccaaacat taccaccaa atatacgag tatgggttg 
      cttccaaga ggcgaagtc ttttcagtg ttttacgag 
      gagcacctt cagcaagcc atagctttg tacaaactg 
      ttctattat gccaaagat tacgacacg ttttatcgg 
      acggccgtg tgggcaaga caacatgta aatgagggc 
      gttttcttg tattctttt tcggtggca atcgtccac 
      cgtgaggac acttacggg atagttttg cccccaatt 
      tacgaaatc tacccccac tatttctac aacaacgag 
      gtgatccag gaggcttac aggtacaaa caacagtat 
      tacaaccag gaacacggt tacacaatc aatgccaat 
      tactccggt ttttaccta aatctgcac cctgaacaa 
      tctttgtcg tacttcact gaagatgta ggtgtcaat 
      tccttctac tactactac aacatttac tacccccat 
      tggttgggt ggggaggac tttgacttt gcccatgac 
      aggcggggt gagcagtac tattacgtg taccaacaa 
      attctggca aggtactac cttgaacgt ttgtccaac 
      gatttcggg gaaataccg tttttcaac tatgaagta 
      ccgttcgaa aatgggtac tacccttta ttgcagtac 
      cccaatggt ctgtttttc ccccaaaga ccgaactat 
      gccaagctt tacgagtac ttctacaac tatgggcaa 
      aaatacggt aataatagg tatgcctac agttatact 
      tttgtccag gattatgag aggaggatt cgggatgcc 
      atcgatcgt ggatacgtc ttttcgcat gatggtcaa 
      aggattaac ttattctcc gaagacggt gttaacatc 
      cttggtaat ttaatcgaa tcaaaccct gactccccc 
      gaccgccac ttctacggc gcccttcac gtgtacgcc 
      cgccacctc ctggggtac tcctcccag cccttggac 
      aaataccac gtggcccct tcggccctc caacactac 
      gaaacgtcg ctccgtgac cccgctttc taccagttc 
      tacaagcgc attgttttg tatttccag aagtacaag 
      tcgtacctc ccgtcctac accgaacac gacttgcat 
      ttccaaggg gtggaggtc aagagtgtt gaattcgac 
      cgacttgtc acctacttc gaccacttt tacaccgat 
      attagcaat gctgtttac gtcacgcct caggagtac 
      gacagcgag aaggtccaa gtgagggta cgacagtac 
      cgcctgaac cacaaaccg ttcacttac cgggttcat 
      gtcagttcg gataaggag caacaagct gttgttaga 
      atttatctg gggcctaaa tatgacgag tacgggcgt 
      tatattaac attagtcac aacagactc aactttgtt 
      gaagttgat cacttcaag taccagctc aaagttggg 
      gaaaatgtg attgagagg aactcccat cagaactac 
      ttctaccag aacgatcgt actagctat cggcagttg 
      tacaagcaa gttttaggc gctttgaac ggcaatgga 
      gagtttaac gtgaacgct aacgaggcc tattttgga 
      tttcctcga agattcctt ttgcctaag ggtaattac 
      gggggtcaa gaattccag ttctttgtc attgtgagc 
      ccatatgtg ccctacaag taccaccaa gagggttat 
      gacgctagc aagtactac tacccaagg gtggggtct 
      ggtgcccat catatcgat aattatgcc tttggttat 
      ccgtttgac cgacccatt cactatgac cagatcttc 
      cacaatgta ccaaatgcc tatttctac actaccaag 
      atttaccat agacatgcc gatgatatc aatgcgtca 
      acttcggcc catcagtga
    mRNA sequence
    • >TC005375 RA
      ttgtttggc cgttcgaac aatcatgag gttcattgt 
      agtggcgat ccttggctg ttgtgccct ggctttggc 
      tttccccga acgtaaaat aaccaataa acaattcct 
      cgagagaca aagagacat ccttcgcct gttccgaca 
      cataaacca gccaagtta ctacaaaga ccaccagga 
      gatagccca gagtttcca cctccatga ccactacga 
      ccactacac taaacctga aattgccaa acattacca 
      ccaaatata cgagtatgg gttgcttcc aagaggcga 
      agtcttttc agtgtttta cgaggagca ccttcagca 
      agccatagc tttgtacaa actgttcta ttatgccaa 
      agattacga cacgtttta tcggacggc cgtgtgggc 
      aagacaaca tgtaaatga gggcgtttt cttgtattc 
      tttttcggt ggcaatcgt ccaccgtga ggacactta 
      cgggatagt tttgccccc aatttacga aatctaccc 
      ccactattt ctacaacaa cgaggtgat ccaggaggc 
      ttacaggta caaacaaca gtattacaa ccaggaaca 
      cggttacac aatcaatgc caattactc cggttttta 
      cctaaatct gcaccctga acaatcttt gtcgtactt 
      cactgaaga tgtaggtgt caattcctt ctactacta 
      ctacaacat ttactaccc ccattggtt gggtgggga 
      ggactttga ctttgccca tgacaggcg gggtgagca 
      gtactatta cgtgtacca acaaattct ggcaaggta 
      ctaccttga acgtttgtc caacgattt cggggaaat 
      accgttttt caactatga agtaccgtt cgaaaatgg 
      gtactaccc tttattgca gtaccccaa tggtctgtt 
      tttccccca aagaccgaa ctatgccaa gctttacga 
      gtacttcta caactatgg gcaaaaata cggtaataa 
      taggtatgc ctacagtta tacttttgt ccaggatta 
      tgagaggag gattcggga tgccatcga tcgtggata 
      cgtcttttc gcatgatgg tcaaaggat taacttatt 
      ctccgaaga cggtgttaa catccttgg taatttaat 
      cgaatcaaa ccctgactc ccccgaccg ccacttcta 
      cggcgccct tcacgtgta cgcccgcca cctcctggg 
      gtactcctc ccagccctt ggacaaata ccacgtggc 
      cccttcggc cctccaaca ctacgaaac gtcgctccg 
      tgaccccgc tttctacca gttctacaa gcgcattgt 
      tttgtattt ccagaagta caagtcgta cctcccgtc 
      ctacaccga acacgactt gcatttcca aggggtgga 
      ggtcaagag tgttgaatt cgaccgact tgtcaccta 
      cttcgacca cttttacac cgatattag caatgctgt 
      ttacgtcac gcctcagga gtacgacag cgagaaggt 
      ccaagtgag ggtacgaca gtaccgcct gaaccacaa 
      accgttcac ttaccgggt tcatgtcag ttcggataa 
      ggagcaaca agctgttgt tagaattta tctggggcc 
      taaatatga cgagtacgg gcgttatat taacattag 
      tcacaacag actcaactt tgttgaagt tgatcactt 
      caagtacca gctcaaagt tggggaaaa tgtgattga 
      gaggaactc ccatcagaa ctacttcta ccagaacga 
      tcgtactag ctatcggca gttgtacaa gcaagtttt 
      aggcgcttt gaacggcaa tggagagtt taacgtgaa 
      cgctaacga ggcctattt tggatttcc tcgaagatt 
      ccttttgcc taagggtaa ttacggggg tcaagaatt 
      ccagttctt tgtcattgt gagcccata tgtgcccta 
      caagtacca ccaagaggg ttatgacgc tagcaagta 
      ctactaccc aagggtggg gtctggtgc ccatcatat 
      cgataatta tgcctttgg ttatccgtt tgaccgacc 
      cattcacta tgaccagat cttccacaa tgtaccaaa 
      tgcctattt ctacactac caagattta ccatagaca 
      tgccgatga tatcaatgc gtcaacttc ggcccatca 
      gtgatcaaa ataacgatt ttgtgtaac taattaact 
      cttgttctg tatttagat taaataaag tgtattcaa 
      aaaa