General information | |||
TC005375 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 13948455..13951493 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC005375 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00838 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
procephalic appendages partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
gnathal appendages partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%
head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax segment sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
ovary without detectable defects - Number of animals showing the phenotype: 4
- >TC005375 RA
MRFIVVAIL GCCALALAF PERKITNKQ FLERQRDIL RLFRHINQP SYYKDHQEI AQSFHLHDH YDHYTKPEI AKHYHQIYE YGLLPRGEV FSVFYEEHL QQAIALYKL FYYAKDYDT FYRTAVWAR QHVNEGVFL YSFSVAIVH REDTYGIVL PPIYEIYPH YFYNNEVIQ EAYRYKQQY YNQEHGYTI NANYSGFYL NLHPEQSLS YFTEDVGVN SFYYYYNIY YPHWLGGED FDFAHDRRG EQYYYVYQQ ILARYYLER LSNDFGEIP FFNYEVPFE NGYYPLLQY PNGLFFPQR PNYAKLYEY FYNYGQKYG NNRYAYSYT FVQDYERRI RDAIDRGYV FSHDGQRIN LFSEDGVNI LGNLIESNP DSPDRHFYG ALHVYARHL LGYSSQPLD KYHVAPSAL QHYETSLRD PAFYQFYKR IVLYFQKYK SYLPSYTEH DLHFQGVEV KSVEFDRLV TYFDHFYTD ISNAVYVTP QEYDSEKVQ VRVRQYRLN HKPFTYRVH VSSDKEQQA VVRIYLGPK YDEYGRYIN ISHNRLNFV EVDHFKYQL KVGENVIER NSHQNYFYQ NDRTSYRQL YKQVLGALN GNGEFNVNA NEAYFGFPR RFLLPKGNY GGQEFQFFV IVSPYVPYK YHQEGYDAS KYYYPRVGS GAHHIDNYA FGYPFDRPI HYDQIFHNV PNAYFYTTK IYHRHADDI NASTSAHQ
- >TC005375 RA
atgaggttc attgtagtg gcgatcctt ggctgttgt gccctggct ttggctttc cccgaacgt aaaataacc aataaacaa ttcctcgag agacaaaga gacatcctt cgcctgttc cgacacata aaccagcca agttactac aaagaccac caggagata gcccagagt ttccacctc catgaccac tacgaccac tacactaaa cctgaaatt gccaaacat taccaccaa atatacgag tatgggttg cttccaaga ggcgaagtc ttttcagtg ttttacgag gagcacctt cagcaagcc atagctttg tacaaactg ttctattat gccaaagat tacgacacg ttttatcgg acggccgtg tgggcaaga caacatgta aatgagggc gttttcttg tattctttt tcggtggca atcgtccac cgtgaggac acttacggg atagttttg cccccaatt tacgaaatc tacccccac tatttctac aacaacgag gtgatccag gaggcttac aggtacaaa caacagtat tacaaccag gaacacggt tacacaatc aatgccaat tactccggt ttttaccta aatctgcac cctgaacaa tctttgtcg tacttcact gaagatgta ggtgtcaat tccttctac tactactac aacatttac tacccccat tggttgggt ggggaggac tttgacttt gcccatgac aggcggggt gagcagtac tattacgtg taccaacaa attctggca aggtactac cttgaacgt ttgtccaac gatttcggg gaaataccg tttttcaac tatgaagta ccgttcgaa aatgggtac tacccttta ttgcagtac cccaatggt ctgtttttc ccccaaaga ccgaactat gccaagctt tacgagtac ttctacaac tatgggcaa aaatacggt aataatagg tatgcctac agttatact tttgtccag gattatgag aggaggatt cgggatgcc atcgatcgt ggatacgtc ttttcgcat gatggtcaa aggattaac ttattctcc gaagacggt gttaacatc cttggtaat ttaatcgaa tcaaaccct gactccccc gaccgccac ttctacggc gcccttcac gtgtacgcc cgccacctc ctggggtac tcctcccag cccttggac aaataccac gtggcccct tcggccctc caacactac gaaacgtcg ctccgtgac cccgctttc taccagttc tacaagcgc attgttttg tatttccag aagtacaag tcgtacctc ccgtcctac accgaacac gacttgcat ttccaaggg gtggaggtc aagagtgtt gaattcgac cgacttgtc acctacttc gaccacttt tacaccgat attagcaat gctgtttac gtcacgcct caggagtac gacagcgag aaggtccaa gtgagggta cgacagtac cgcctgaac cacaaaccg ttcacttac cgggttcat gtcagttcg gataaggag caacaagct gttgttaga atttatctg gggcctaaa tatgacgag tacgggcgt tatattaac attagtcac aacagactc aactttgtt gaagttgat cacttcaag taccagctc aaagttggg gaaaatgtg attgagagg aactcccat cagaactac ttctaccag aacgatcgt actagctat cggcagttg tacaagcaa gttttaggc gctttgaac ggcaatgga gagtttaac gtgaacgct aacgaggcc tattttgga tttcctcga agattcctt ttgcctaag ggtaattac gggggtcaa gaattccag ttctttgtc attgtgagc ccatatgtg ccctacaag taccaccaa gagggttat gacgctagc aagtactac tacccaagg gtggggtct ggtgcccat catatcgat aattatgcc tttggttat ccgtttgac cgacccatt cactatgac cagatcttc cacaatgta ccaaatgcc tatttctac actaccaag atttaccat agacatgcc gatgatatc aatgcgtca acttcggcc catcagtga
- >TC005375 RA
ttgtttggc cgttcgaac aatcatgag gttcattgt agtggcgat ccttggctg ttgtgccct ggctttggc tttccccga acgtaaaat aaccaataa acaattcct cgagagaca aagagacat ccttcgcct gttccgaca cataaacca gccaagtta ctacaaaga ccaccagga gatagccca gagtttcca cctccatga ccactacga ccactacac taaacctga aattgccaa acattacca ccaaatata cgagtatgg gttgcttcc aagaggcga agtcttttc agtgtttta cgaggagca ccttcagca agccatagc tttgtacaa actgttcta ttatgccaa agattacga cacgtttta tcggacggc cgtgtgggc aagacaaca tgtaaatga gggcgtttt cttgtattc tttttcggt ggcaatcgt ccaccgtga ggacactta cgggatagt tttgccccc aatttacga aatctaccc ccactattt ctacaacaa cgaggtgat ccaggaggc ttacaggta caaacaaca gtattacaa ccaggaaca cggttacac aatcaatgc caattactc cggttttta cctaaatct gcaccctga acaatcttt gtcgtactt cactgaaga tgtaggtgt caattcctt ctactacta ctacaacat ttactaccc ccattggtt gggtgggga ggactttga ctttgccca tgacaggcg gggtgagca gtactatta cgtgtacca acaaattct ggcaaggta ctaccttga acgtttgtc caacgattt cggggaaat accgttttt caactatga agtaccgtt cgaaaatgg gtactaccc tttattgca gtaccccaa tggtctgtt tttccccca aagaccgaa ctatgccaa gctttacga gtacttcta caactatgg gcaaaaata cggtaataa taggtatgc ctacagtta tacttttgt ccaggatta tgagaggag gattcggga tgccatcga tcgtggata cgtcttttc gcatgatgg tcaaaggat taacttatt ctccgaaga cggtgttaa catccttgg taatttaat cgaatcaaa ccctgactc ccccgaccg ccacttcta cggcgccct tcacgtgta cgcccgcca cctcctggg gtactcctc ccagccctt ggacaaata ccacgtggc cccttcggc cctccaaca ctacgaaac gtcgctccg tgaccccgc tttctacca gttctacaa gcgcattgt tttgtattt ccagaagta caagtcgta cctcccgtc ctacaccga acacgactt gcatttcca aggggtgga ggtcaagag tgttgaatt cgaccgact tgtcaccta cttcgacca cttttacac cgatattag caatgctgt ttacgtcac gcctcagga gtacgacag cgagaaggt ccaagtgag ggtacgaca gtaccgcct gaaccacaa accgttcac ttaccgggt tcatgtcag ttcggataa ggagcaaca agctgttgt tagaattta tctggggcc taaatatga cgagtacgg gcgttatat taacattag tcacaacag actcaactt tgttgaagt tgatcactt caagtacca gctcaaagt tggggaaaa tgtgattga gaggaactc ccatcagaa ctacttcta ccagaacga tcgtactag ctatcggca gttgtacaa gcaagtttt aggcgcttt gaacggcaa tggagagtt taacgtgaa cgctaacga ggcctattt tggatttcc tcgaagatt ccttttgcc taagggtaa ttacggggg tcaagaatt ccagttctt tgtcattgt gagcccata tgtgcccta caagtacca ccaagaggg ttatgacgc tagcaagta ctactaccc aagggtggg gtctggtgc ccatcatat cgataatta tgcctttgg ttatccgtt tgaccgacc cattcacta tgaccagat cttccacaa tgtaccaaa tgcctattt ctacactac caagattta ccatagaca tgccgatga tatcaatgc gtcaacttc ggcccatca gtgatcaaa ataacgatt ttgtgtaac taattaact cttgttctg tatttagat taaataaag tgtattcaa aaaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
