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Gene information
General information
TC005372 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13969276..13970208
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005372
    iBeetle screen iB_00837

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 leg musculature mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    leg musculature coxal musculature partially present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005372 RA
      MAFSAHFFT FLAIIITSH LTLCQEPEV VEDISSNLT 
      SFTNSTHNA TLTNTTESS SNKTLKLVQ CLPDFGAPE 
      VQLVNDTEL IKLLFTQSN ITSRDTPAG CILVLFYSK 
      YCPFSSMAA PHFNALPRA FPDIKMVAI NALAYRLFN 
      TQNGIVGVP TLMLFHNGR PVAKFNESE YTLELFSKF 
      LAKHTGIAA AEKSIVTSA DFKGPVTSV PAREFDIFL 
      IISWVFIAL CGAYYFTKS KCWGWIVET IQSNWRESE 
      AHAQHEHAD
    CDS sequence
    • >TC005372 RA
      atggctttt tctgcacat tttttcaca ttcctcgcg 
      ataatcata acctcacac ttgacgtta tgtcaggag 
      cccgaagtt gtggaagac atctccagc aacctcacc 
      tccttcacc aactccacc cacaacgca actctcaca 
      aacaccact gaatcatcc agcaacaaa accctcaaa 
      ctcgtgcaa tgtttgccc gattttggg gcccccgaa 
      gtccaacta gtcaacgac accgagctc attaaactc 
      ctcttcacc cagagcaac atcacgagc cgggacacc 
      cccgccggg tgcatccta gtgctgttc tactcgaaa 
      tattgcccg ttttccagc atggcagcc ccccatttt 
      aacgctctg ccaagggct ttccccgat attaaaatg 
      gtggctatc aacgcgctg gcttatcgg ttgtttaat 
      acgcagaac gggattgtg ggggttccc acgttaatg 
      ttgtttcat aacgggaga cccgttgct aaatttaat 
      gagtcggag tatacactt gagcttttt tccaaattt 
      ttggccaaa catacgggg attgcggct gccgaaaag 
      tcaattgtg acctcggct gatttcaag gggcctgtc 
      accagtgtc cctgcgcgc gaatttgat atttttttg 
      atcatttcc tgggttttt attgctctg tgtggggct 
      tattatttc acgaagtcc aagtgctgg gggtggatt 
      gttgagacc attcagagc aactggagg gagtcggag 
      gcacacgcc cagcatgag catgccgat tag
    mRNA sequence
    • >TC005372 RA
      tttctgtat cgcttgttt taacccaac caaatcaat 
      aattcgaaa tgataaaat aagacacga aatggcttt 
      ttctgcaca ttttttcac attcctcgc gataatcat 
      aacctcaca cttgacgtt atgtcagga gcccgaagt 
      tgtggaaga catctccag caacctcac ctccttcac 
      caactccac ccacaacgc aactctcac aaacaccac 
      tgaatcatc cagcaacaa aaccctcaa actcgtgca 
      atgtttgcc cgattttgg ggcccccga agtccaact 
      agtcaacga caccgagct cattaaact cctcttcac 
      ccagagcaa catcacgag ccgggacac ccccgccgg 
      gtgcatcct agtgctgtt ctactcgaa atattgccc 
      gttttccag catggcagc cccccattt taacgctct 
      gccaagggc tttccccga tattaaaat ggtggctat 
      caacgcgct ggcttatcg gttgtttaa tacgcagaa 
      cgggattgt gggggttcc cacgttaat gttgtttca 
      taacgggag acccgttgc taaatttaa tgagtcgga 
      gtatacact tgagctttt ttccaaatt tttggccaa 
      acatacggg gattgcggc tgccgaaaa gtcaattgt 
      gacctcggc tgatttcaa ggggcctgt caccagtgt 
      ccctgcgcg cgaatttga tattttttt gatcatttc 
      ctgggtttt tattgctct gtgtggggc ttattattt 
      cacgaagtc caagtgctg ggggtggat tgttgagac 
      cattcagag caactggag ggagtcgga ggcacacgc 
      ccagcatga gcatgccga ttaggccct tttttgttt 
      aaataaatt atttttatt gaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY