Status
Please wait ...
Gene information
General information
TC005369 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid58: 822415..827043
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005369
    iBeetle screen iB_00836
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 7 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005369 RA
      MDKYEQISV VGEGSYGLV MKCRHKETD QIVAIKKFL 
      ETEEDATIR KMALREIRM LKRLKHENL VTMIEVFRH 
      RKRFFLVFE FLEGTVLDE LEKMPGGLG DERCRERIY 
      QVTRAINYC HSNNIIHRD VKPENVLVS SLGVVKLCD 
      FGFARLVCI SGEPCTEYV ATRWYRAPE LLVGESNYG 
      APVDIWAIG CLFAEMMTG DPLFPGESD IDQLYLIVK 
      MLGKPCHRH QHLMSRSSH LRGMIKSAS SETDLGKLF 
      PSWSLFALD FLASCMKMD PHERSTSEE LLKSSYFTH 
      DRFPQKFLP ALREKVNTE FNNNPLLRK YKTEILMST 
      DKKEETKRR GGDQIKWRF SLAEGSIKR KYSYDVFNE 
      SDKNLISLS KTSQRLNVV QKSGQKPQK PSGSNAEMQ 
      MLEKSLESL AKFSERPVS ARKDSPLPP SPPEFQSLQ 
      QFSDFNKSP NVLHPSINN ISFNRDPPK KSPNLLQSL 
      TVKSNLNQV PLLTNPRTQ FLKKIESSV TSDPPWLGF 
      PKKNKEQKA RNDVFTLPN LPGATGSPN KNRKKGEAE 
      LNITVTPRI QSSTSQKLS PSKNSSSFP MM
    CDS sequence
    • >TC005369 RA
      atggacaaa tacgagcaa atttcggtc gttggggaa 
      ggctcttac ggcctggtg atgaagtgc aggcacaag 
      gaaaccgac caaatcgtc gctattaaa aagttcctc 
      gaaaccgaa gaggacgct acaatccgc aaaatggcc 
      ctgcgagaa attcgcatg ctcaaaaga ctgaaacat 
      gaaaatctc gtcacaatg atcgaggtt ttccgccac 
      cggaagcgc tttttcctc gtttttgag tttctggaa 
      gggacggtt ttagacgag ctggagaaa atgcccgga 
      gggctggga gatgagcgg tgtcgggag aggatatac 
      caagtcacc agagccatc aactactgt cacagcaat 
      aacattatt catcgtgac gtgaaaccg gaaaacgtg 
      ctagtttcc tccctcggc gttgtgaaa ctttgcgat 
      tttggtttc gcgcgtctt gtgtgcatc tcgggggag 
      ccttgcacc gagtacgtg gccaccagg tggtaccgt 
      gcccctgaa ctcctggtg ggcgagtca aactatggc 
      gccccggtc gatatttgg gccattggc tgcctcttc 
      gccgagatg atgaccgga gacccgttg tttcccggt 
      gagagtgac atcgatcaa ctgtacctc atcgtcaaa 
      atgttgggc aaaccctgc cacaggcac caacacctc 
      atgtccagg agttcgcac ctccgtgga atgatcaaa 
      tcagcgtcc agtgagacg gacttgggc aaactcttc 
      cccagctgg tcgctcttc gccctcgac ttcctggcc 
      tcttgcatg aaaatggac ccccacgag agatccacg 
      tcggaggag ttgttgaaa agtagttat ttcacccat 
      gataggttc ccccagaag tttttacca gcgttacgc 
      gagaaggtc aacaccgaa tttaacaat aacccactt 
      ttgcggaaa tacaagact gagattttg atgtcaacg 
      gacaagaaa gaagagaca aaacgacgg ggaggcgac 
      caaatcaag tggcgcttt agcctggct gagggaagc 
      atcaagcgg aaatatagt tacgacgtt ttcaacgaa 
      agtgataag aacttgatt agtttatcg aaaacaagc 
      cagagactt aacgtggtg caaaaatcc ggtcaaaaa 
      ccccaaaaa ccgagcggt agcaacgcc gaaatgcaa 
      atgcttgaa aaatcgctc gaaagtttg gccaagttc 
      agtgagcgt ccagtctcg gcccggaaa gacagtccc 
      ttgccccca tcgcccccc gaattccag tccttgcaa 
      cagttcagt gactttaat aaatccccc aatgtcttg 
      caccccagc atcaacaac attagtttc aacagagac 
      cccccgaaa aaatccccc aacttgttg caaagttta 
      acagtgaaa tcgaacttg aatcaagtg cccctattg 
      acaaatccc cggactcag tttttgaaa aaaatcgag 
      agtagtgtg acgtcagat cccccgtgg ctgggattc 
      cccaagaaa aataaggag caaaaggcg cgaaacgat 
      gtcttcacg cttccaaat ttacctgga gctacaggg 
      agtccgaat aaaaaccgg aagaagggg gaagcggag 
      ctgaacatc accgtgact ccaagaata caatcatct 
      acctcacaa aaactctca ccatccaaa aactcgagt 
      agttttcct atgatgtga
    mRNA sequence
    • >TC005369 RA
      ccagtcggt ggaaagcgt cgtgaaaag cgggccgct 
      tggtgcact ggaaaaggt gaaagttca tggtcttgt 
      gcaaaatgc aatcctgga attttcagc cacatcacg 
      acgaaatta tttcgcgca aatgatgag ttgaaacgc 
      cgccatgga caaatacga gcaaatttc ggtcgttgg 
      ggaaggctc ttacggcct ggtgatgaa gtgcaggca 
      caaggaaac cgaccaaat cgtcgctat taaaaagtt 
      cctcgaaac cgaagagga cgctacaat ccgcaaaat 
      ggccctgcg agaaattcg catgctcaa aagactgaa 
      acatgaaaa tctcgtcac aatgatcga ggttttccg 
      ccaccggaa gcgcttttt cctcgtttt tgagtttct 
      ggaagggac ggttttaga cgagctgga gaaaatgcc 
      cggagggct gggagatga gcggtgtcg ggagaggat 
      ataccaagt caccagagc catcaacta ctgtcacag 
      caataacat tattcatcg tgacgtgaa accggaaaa 
      cgtgctagt ttcctccct cggcgttgt gaaactttg 
      cgattttgg tttcgcgcg tcttgtgtg catctcggg 
      ggagccttg caccgagta cgtggccac caggtggta 
      ccgtgcccc tgaactcct ggtgggcga gtcaaacta 
      tggcgcccc ggtcgatat ttgggccat tggctgcct 
      cttcgccga gatgatgac cggagaccc gttgtttcc 
      cggtgagag tgacatcga tcaactgta cctcatcgt 
      caaaatgtt gggcaaacc ctgccacag gcaccaaca 
      cctcatgtc caggagttc gcacctccg tggaatgat 
      caaatcagc gtccagtga gacggactt gggcaaact 
      cttccccag ctggtcgct cttcgccct cgacttcct 
      ggcctcttg catgaaaat ggaccccca cgagagatc 
      cacgtcgga ggagttgtt gaaaagtag ttatttcac 
      ccatgatag gttccccca gaagttttt accagcgtt 
      acgcgagaa ggtcaacac cgaatttaa caataaccc 
      acttttgcg gaaatacaa gactgagat tttgatgtc 
      aacggacaa gaaagaaga gacaaaacg acggggagg 
      cgaccaaat caagtggcg ctttagcct ggctgaggg 
      aagcatcaa gcggaaata tagttacga cgttttcaa 
      cgaaagtga taagaactt gattagttt atcgaaaac 
      aagccagag acttaacgt ggtgcaaaa atccggtca 
      aaaacccca aaaaccgag cggtagcaa cgccgaaat 
      gcaaatgct tgaaaaatc gctcgaaag tttggccaa 
      gttcagtga gcgtccagt ctcggcccg gaaagacag 
      tcccttgcc cccatcgcc ccccgaatt ccagtcctt 
      gcaacagtt cagtgactt taataaatc ccccaatgt 
      cttgcaccc cagcatcaa caacattag tttcaacag 
      agacccccc gaaaaaatc ccccaactt gttgcaaag 
      tttaacagt gaaatcgaa cttgaatca agtgcccct 
      attgacaaa tccccggac tcagttttt gaaaaaaat 
      cgagagtag tgtgacgtc agatccccc gtggctggg 
      attccccaa gaaaaataa ggagcaaaa ggcgcgaaa 
      cgatgtctt cacgcttcc aaatttacc tggagctac 
      agggagtcc gaataaaaa ccggaagaa gggggaagc 
      ggagctgaa catcaccgt gactccaag aatacaatc 
      atctacctc acaaaaact ctcaccatc caaaaactc 
      gagtagttt tcctatgat gtgatttag ttattgatt 
      tgcttgtgt aaatagggc gtcttgtaa aacttgttt 
      ctattctag gcctacaga tataagaaa gcaaaacgg 
      aaactcaat attttggat atttcgctc cctttgtct 
      gttaaggag aaaggagat gtttaattt tttatcata 
      gacccaact attatgtac catgttata ttatttaaa 
      taaaggata tatttatat