| General information | |||
| TC005357 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 14106816..14111510 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC005357 | ||
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00834 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)
adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC005357 RA
MASNVPEKN EENVPDWVL AKRGINFLI NNNAKEAQE LFSSYPESL PMCAGYSFA AFMDALMTF EDDKLNLAT SVLKEVERK CTSETGWFK SVKKAFGSS DNNSLAENL ETQIILADS QVCLAILTF LQQDISGYF KGGWVLRKA WKVYQGTYQ EILHLYKEA GGEVGAPLP EVVMEPLSP GECSSTGSE ASKSDPLNG YTKKIPYSH SAILTHTQT DSSSGCSTP NSMKNNCSR SSIDASALS FLKKSLSVN SALSKHTAK AEWSLSLAY FTSTFHLFS PTDKLRSAQ VDKETIERL MGAVSFGYG LFQLGISLL PPSLLRLTN FLGFGGNRQ NGIACLMYA REGIDMRAP LATLSLLWY HTIVRPFYA LDGSNVQAG VNAATQLLQ ESEGEYGQS ALFLFFKGR VNRLNSDIP NALKSFQSS VENATQREI KILALHEVG WCHLIQLDY CNAENTFSY LKSWSRWSR AFYSYLAGI CCGSCRNSS SLTNIKEIK TGVTLGTKG NQLDEFLSR RAKCCPSDD DELSTLPAI FWKLLVYEM LYLWNALPS CLPQNIEQI KRDCEQVGD GMEPMLGLS KLILGCCNC IQRQFSAGI ANFRQCLDL RKGCPNNAV DAHVSAFSQ YELGALLIK TNETKAEGK LLLQNIAQY KDYDFEQRL NVRVHSMLK HL
- >TC005357 RA
atggcttca aacgtcccg gaaaaaaac gaagaaaac gtgcctgat tgggtcctg gcaaagcgg ggaatcaac tttctaatc aacaacaat gctaaagaa gcccaagag ttgttttct tcgtatccg gagagtcta ccaatgtgt gctggctac tcatttgcc gcgttcatg gatgcctta atgaccttc gaagacgac aaactcaac ctggcaact tccgtcctc aaggaagtt gaacgaaag tgcacttct gaaaccggt tggttcaaa tcggtcaaa aaagcgttc ggttccagc gacaacaac tcgcttgcc gaaaatcta gaaacgcag ataattcta gccgactcg caagtgtgt ctggcgatc ttgactttc ctgcagcag gacatttcc gggtatttc aagggcggc tgggtgctg aggaaggcc tggaaggtg taccaaggc acctaccag gagatcctc cacttgtac aaggaggca ggcggcgaa gtcggcgcc cctttacca gaagttgtg atggagccg ctcagtcca ggcgagtgc agctccact ggttcggaa gccagcaag tccgatcct ctaaacggg tataccaaa aaaatccct tactcccac tccgccatc ctcacacac acccaaacc gactcctcc tccggctgc tccaccccc aactcaatg aagaacaac tgtagcaga tctagcata gatgcgtct gcactttcg ttcctcaaa aaatcgctc tcggtcaat agcgcgctc tcaaaacac acggccaag gccgaatgg tcgctctct ttggcttac ttcacttca acttttcac ctattttca ccaacagac aaactgaga agtgctcaa gttgacaag gaaacgatc gagcgctta atgggggct gtgtcgttc ggctatggt ttgttccag ttggggatt tccctactg ccgccgtcc ctgctcaga cttacgaat tttctgggg tttggaggc aacagacag aatggcatt gcctgtctg atgtatgca agggaaggg attgacatg agggcaccg ctagcaact ttgtcgtta ctttggtac catactata gttaggccg ttttatgct cttgatggc agtaatgtt caagctggg gttaatgcc gccactcag ttgctacaa gaatcggaa ggagagtat ggacaaagt gcccttttt ttgtttttc aaagggcgg gtcaatcgg ttaaatagc gacattccc aacgctttg aaatccttc caatcgtca gtcgagaac gcaactcag cgcgaaatc aaaattttg gcccttcat gaagtaggt tggtgccat ttgatccaa ctggattac tgtaacgcc gaaaacact ttctcgtat ttgaagtct tggagtcgt tggagtcgc gcattttac agttacttg gctgggatt tgttgcggt tcgtgtcgc aattcgagc agtttgacc aacattaag gaaatcaaa acgggagtc acccttggt actaagggc aaccaactg gatgagttt ctctcccga cgtgccaaa tgttgtccg tccgatgac gacgaactg tccactttg ccagccatt ttttggaaa ttgctagtt tacgaaatg ctttattta tggaacgct ttaccgagc tgtttaccg caaaatatc gaacagatt aaacgagac tgtgagcaa gtgggggat gggatggag ccgatgttg ggcttgtct aagctgatt ctgggctgt tgtaactgc attcaaagg cagttttct gcaggaatt gccaatttt cggcagtgt ctggacttg aggaaaggg tgtcccaac aatgcagtc gatgcccat gtttccgct ttctcgcaa tacgaattg ggggcgttg ttaattaaa actaacgaa acgaaagct gagggtaaa ttgttgctt caaaacata gctcagtac aaagactac gattttgag cagcggctc aatgtgaga gtccactca atgctgaaa catctgtag
- >TC005357 RA
acatttctg tcaaaacac ccccaaaat tcccagtga acatccctc caaaatgcc acactaaat caggcagag atggcttca aacgtcccg gaaaaaaac gaagaaaac gtgcctgat tgggtcctg gcaaagcgg ggaatcaac tttctaatc aacaacaat gctaaagaa gcccaagag ttgttttct tcgtatccg gagagtcta ccaatgtgt gctggctac tcatttgcc gcgttcatg gatgcctta atgaccttc gaagacgac aaactcaac ctggcaact tccgtcctc aaggaagtt gaacgaaag tgcacttct gaaaccggt tggttcaaa tcggtcaaa aaagcgttc ggttccagc gacaacaac tcgcttgcc gaaaatcta gaaacgcag ataattcta gccgactcg caagtgtgt ctggcgatc ttgactttc ctgcagcag gacatttcc gggtatttc aagggcggc tgggtgctg aggaaggcc tggaaggtg taccaaggc acctaccag gagatcctc cacttgtac aaggaggca ggcggcgaa gtcggcgcc cctttacca gaagttgtg atggagccg ctcagtcca ggcgagtgc agctccact ggttcggaa gccagcaag tccgatcct ctaaacggg tataccaaa aaaatccct tactcccac tccgccatc ctcacacac acccaaacc gactcctcc tccggctgc tccaccccc aactcaatg aagaacaac tgtagcaga tctagcata gatgcgtct gcactttcg ttcctcaaa aaatcgctc tcggtcaat agcgcgctc tcaaaacac acggccaag gccgaatgg tcgctctct ttggcttac ttcacttca acttttcac ctattttca ccaacagac aaactgaga agtgctcaa gttgacaag gaaacgatc gagcgctta atgggggct gtgtcgttc ggctatggt ttgttccag ttggggatt tccctactg ccgccgtcc ctgctcaga cttacgaat tttctgggg tttggaggc aacagacag aatggcatt gcctgtctg atgtatgca agggaaggg attgacatg agggcaccg ctagcaact ttgtcgtta ctttggtac catactata gttaggccg ttttatgct cttgatggc agtaatgtt caagctggg gttaatgcc gccactcag ttgctacaa gaatcggaa ggagagtat ggacaaagt gcccttttt ttgtttttc aaagggcgg gtcaatcgg ttaaatagc gacattccc aacgctttg aaatccttc caatcgtca gtcgagaac gcaactcag cgcgaaatc aaaattttg gcccttcat gaagtaggt tggtgccat ttgatccaa ctggattac tgtaacgcc gaaaacact ttctcgtat ttgaagtct tggagtcgt tggagtcgc gcattttac agttacttg gctgggatt tgttgcggt tcgtgtcgc aattcgagc agtttgacc aacattaag gaaatcaaa acgggagtc acccttggt actaagggc aaccaactg gatgagttt ctctcccga cgtgccaaa tgttgtccg tccgatgac gacgaactg tccactttg ccagccatt ttttggaaa ttgctagtt tacgaaatg ctttattta tggaacgct ttaccgagc tgtttaccg caaaatatc gaacagatt aaacgagac tgtgagcaa gtgggggat gggatggag ccgatgttg ggcttgtct aagctgatt ctgggctgt tgtaactgc attcaaagg cagttttct gcaggaatt gccaatttt cggcagtgt ctggacttg aggaaaggg tgtcccaac aatgcagtc gatgcccat gtttccgct ttctcgcaa tacgaattg ggggcgttg ttaattaaa actaacgaa acgaaagct gagggtaaa ttgttgctt caaaacata gctcagtac aaagactac gattttgag cagcggctc aatgtgaga gtccactca atgctgaaa catctgtag accaagaac tagtgacaa tattacgaa ttaatgtaa tataacaat aataccgat
