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Gene information
General information
TC005357 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14106816..14111510
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC005357
      iBeetle screen iB_00834

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
      Days post injection

      adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection

      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC005357 RA
        MASNVPEKN EENVPDWVL AKRGINFLI NNNAKEAQE 
LFSSYPESL PMCAGYSFA AFMDALMTF EDDKLNLAT 
SVLKEVERK CTSETGWFK SVKKAFGSS DNNSLAENL 
ETQIILADS QVCLAILTF LQQDISGYF KGGWVLRKA 
WKVYQGTYQ EILHLYKEA GGEVGAPLP EVVMEPLSP 
GECSSTGSE ASKSDPLNG YTKKIPYSH SAILTHTQT 
DSSSGCSTP NSMKNNCSR SSIDASALS FLKKSLSVN 
SALSKHTAK AEWSLSLAY FTSTFHLFS PTDKLRSAQ 
VDKETIERL MGAVSFGYG LFQLGISLL PPSLLRLTN 
FLGFGGNRQ NGIACLMYA REGIDMRAP LATLSLLWY 
HTIVRPFYA LDGSNVQAG VNAATQLLQ ESEGEYGQS 
ALFLFFKGR VNRLNSDIP NALKSFQSS VENATQREI 
KILALHEVG WCHLIQLDY CNAENTFSY LKSWSRWSR 
AFYSYLAGI CCGSCRNSS SLTNIKEIK TGVTLGTKG 
NQLDEFLSR RAKCCPSDD DELSTLPAI FWKLLVYEM 
LYLWNALPS CLPQNIEQI KRDCEQVGD GMEPMLGLS 
KLILGCCNC IQRQFSAGI ANFRQCLDL RKGCPNNAV 
DAHVSAFSQ YELGALLIK TNETKAEGK LLLQNIAQY 
KDYDFEQRL NVRVHSMLK HL
      CDS sequence
      • >TC005357 RA
        atggcttca aacgtcccg gaaaaaaac gaagaaaac 
gtgcctgat tgggtcctg gcaaagcgg ggaatcaac 
tttctaatc aacaacaat gctaaagaa gcccaagag 
ttgttttct tcgtatccg gagagtcta ccaatgtgt 
gctggctac tcatttgcc gcgttcatg gatgcctta 
atgaccttc gaagacgac aaactcaac ctggcaact 
tccgtcctc aaggaagtt gaacgaaag tgcacttct 
gaaaccggt tggttcaaa tcggtcaaa aaagcgttc 
ggttccagc gacaacaac tcgcttgcc gaaaatcta 
gaaacgcag ataattcta gccgactcg caagtgtgt 
ctggcgatc ttgactttc ctgcagcag gacatttcc 
gggtatttc aagggcggc tgggtgctg aggaaggcc 
tggaaggtg taccaaggc acctaccag gagatcctc 
cacttgtac aaggaggca ggcggcgaa gtcggcgcc 
cctttacca gaagttgtg atggagccg ctcagtcca 
ggcgagtgc agctccact ggttcggaa gccagcaag 
tccgatcct ctaaacggg tataccaaa aaaatccct 
tactcccac tccgccatc ctcacacac acccaaacc 
gactcctcc tccggctgc tccaccccc aactcaatg 
aagaacaac tgtagcaga tctagcata gatgcgtct 
gcactttcg ttcctcaaa aaatcgctc tcggtcaat 
agcgcgctc tcaaaacac acggccaag gccgaatgg 
tcgctctct ttggcttac ttcacttca acttttcac 
ctattttca ccaacagac aaactgaga agtgctcaa 
gttgacaag gaaacgatc gagcgctta atgggggct 
gtgtcgttc ggctatggt ttgttccag ttggggatt 
tccctactg ccgccgtcc ctgctcaga cttacgaat 
tttctgggg tttggaggc aacagacag aatggcatt 
gcctgtctg atgtatgca agggaaggg attgacatg 
agggcaccg ctagcaact ttgtcgtta ctttggtac 
catactata gttaggccg ttttatgct cttgatggc 
agtaatgtt caagctggg gttaatgcc gccactcag 
ttgctacaa gaatcggaa ggagagtat ggacaaagt 
gcccttttt ttgtttttc aaagggcgg gtcaatcgg 
ttaaatagc gacattccc aacgctttg aaatccttc 
caatcgtca gtcgagaac gcaactcag cgcgaaatc 
aaaattttg gcccttcat gaagtaggt tggtgccat 
ttgatccaa ctggattac tgtaacgcc gaaaacact 
ttctcgtat ttgaagtct tggagtcgt tggagtcgc 
gcattttac agttacttg gctgggatt tgttgcggt 
tcgtgtcgc aattcgagc agtttgacc aacattaag 
gaaatcaaa acgggagtc acccttggt actaagggc 
aaccaactg gatgagttt ctctcccga cgtgccaaa 
tgttgtccg tccgatgac gacgaactg tccactttg 
ccagccatt ttttggaaa ttgctagtt tacgaaatg 
ctttattta tggaacgct ttaccgagc tgtttaccg 
caaaatatc gaacagatt aaacgagac tgtgagcaa 
gtgggggat gggatggag ccgatgttg ggcttgtct 
aagctgatt ctgggctgt tgtaactgc attcaaagg 
cagttttct gcaggaatt gccaatttt cggcagtgt 
ctggacttg aggaaaggg tgtcccaac aatgcagtc 
gatgcccat gtttccgct ttctcgcaa tacgaattg 
ggggcgttg ttaattaaa actaacgaa acgaaagct 
gagggtaaa ttgttgctt caaaacata gctcagtac 
aaagactac gattttgag cagcggctc aatgtgaga 
gtccactca atgctgaaa catctgtag
      mRNA sequence
      • >TC005357 RA
        acatttctg tcaaaacac ccccaaaat tcccagtga 
acatccctc caaaatgcc acactaaat caggcagag 
atggcttca aacgtcccg gaaaaaaac gaagaaaac 
gtgcctgat tgggtcctg gcaaagcgg ggaatcaac 
tttctaatc aacaacaat gctaaagaa gcccaagag 
ttgttttct tcgtatccg gagagtcta ccaatgtgt 
gctggctac tcatttgcc gcgttcatg gatgcctta 
atgaccttc gaagacgac aaactcaac ctggcaact 
tccgtcctc aaggaagtt gaacgaaag tgcacttct 
gaaaccggt tggttcaaa tcggtcaaa aaagcgttc 
ggttccagc gacaacaac tcgcttgcc gaaaatcta 
gaaacgcag ataattcta gccgactcg caagtgtgt 
ctggcgatc ttgactttc ctgcagcag gacatttcc 
gggtatttc aagggcggc tgggtgctg aggaaggcc 
tggaaggtg taccaaggc acctaccag gagatcctc 
cacttgtac aaggaggca ggcggcgaa gtcggcgcc 
cctttacca gaagttgtg atggagccg ctcagtcca 
ggcgagtgc agctccact ggttcggaa gccagcaag 
tccgatcct ctaaacggg tataccaaa aaaatccct 
tactcccac tccgccatc ctcacacac acccaaacc 
gactcctcc tccggctgc tccaccccc aactcaatg 
aagaacaac tgtagcaga tctagcata gatgcgtct 
gcactttcg ttcctcaaa aaatcgctc tcggtcaat 
agcgcgctc tcaaaacac acggccaag gccgaatgg 
tcgctctct ttggcttac ttcacttca acttttcac 
ctattttca ccaacagac aaactgaga agtgctcaa 
gttgacaag gaaacgatc gagcgctta atgggggct 
gtgtcgttc ggctatggt ttgttccag ttggggatt 
tccctactg ccgccgtcc ctgctcaga cttacgaat 
tttctgggg tttggaggc aacagacag aatggcatt 
gcctgtctg atgtatgca agggaaggg attgacatg 
agggcaccg ctagcaact ttgtcgtta ctttggtac 
catactata gttaggccg ttttatgct cttgatggc 
agtaatgtt caagctggg gttaatgcc gccactcag 
ttgctacaa gaatcggaa ggagagtat ggacaaagt 
gcccttttt ttgtttttc aaagggcgg gtcaatcgg 
ttaaatagc gacattccc aacgctttg aaatccttc 
caatcgtca gtcgagaac gcaactcag cgcgaaatc 
aaaattttg gcccttcat gaagtaggt tggtgccat 
ttgatccaa ctggattac tgtaacgcc gaaaacact 
ttctcgtat ttgaagtct tggagtcgt tggagtcgc 
gcattttac agttacttg gctgggatt tgttgcggt 
tcgtgtcgc aattcgagc agtttgacc aacattaag 
gaaatcaaa acgggagtc acccttggt actaagggc 
aaccaactg gatgagttt ctctcccga cgtgccaaa 
tgttgtccg tccgatgac gacgaactg tccactttg 
ccagccatt ttttggaaa ttgctagtt tacgaaatg 
ctttattta tggaacgct ttaccgagc tgtttaccg 
caaaatatc gaacagatt aaacgagac tgtgagcaa 
gtgggggat gggatggag ccgatgttg ggcttgtct 
aagctgatt ctgggctgt tgtaactgc attcaaagg 
cagttttct gcaggaatt gccaatttt cggcagtgt 
ctggacttg aggaaaggg tgtcccaac aatgcagtc 
gatgcccat gtttccgct ttctcgcaa tacgaattg 
ggggcgttg ttaattaaa actaacgaa acgaaagct 
gagggtaaa ttgttgctt caaaacata gctcagtac 
aaagactac gattttgag cagcggctc aatgtgaga 
gtccactca atgctgaaa catctgtag accaagaac 
tagtgacaa tattacgaa ttaatgtaa tataacaat 
aataccgat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY