Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00833 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: > 50
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present -
Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide -
Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: bad quality of preparation,
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
| Comment: metathorax and abdomen not present |
| Comment: metathorax and abdomen not present |
remarks: bad quality of preparation,
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
Days post injection
- >TC034188 RA
MLTIILSVV LGALIYYKL IKPASFWEE RNITHASAW PLIGSMWES VIQKKNFNE ISADIYKKY PNDRYVGYM QFSNPALLI RDLNLIKQI GVKAFDHFH DHLAFVGVK AEPLFSKSL AILQGEEWK QMRATLSPV FTSSKMKNM YHLIYECAE NFAKHFQGR KEDVEVKDI FSKFTNDVI ATAAFGIQI NSLEEPNNE FYLMGKLLT TFKGWQLIK FFFAQSVPR IFELTKLNI FNQKVIKYF YDIIIDNIH KREKEGIIR PDLIHLLME ARKGKLKHD NSNEGGDGF ATVEESDIG KNSKQLELT EDLMVAQAL IFFFAGFET ISTSFSFTA YELATNPDV QKKLQKEID SAFQENHGK ISYNVLQSM KYLDQVVSG GYFHCLKFF YDFFAESLR LWPPAPQTD RFCNTDFVL EPTKPDERR FTIEKGVTT IIPIYGIHR DPQYFPNPD KFDPERFSD ENKAKIVPG TYMPFGVGP RNCIGSRLA LLELKTLFF HLLSKCDIV TNSKTVIPL KISPNNGNI TPKEGIHLS FKPRKINGK IFRRLIISP LTKMLTTIL LTLLSALIY FKLIKPASF WETRNVPHV TSWPLVGSM FPVLTQKKH FVEISQNIY TQFPNAKCV GYVLFTKPT LLIRDLDLL KQIGVKEFD SFHDHLPFV TEATEPLLS KSLLNLLGD EWRKMRATL SPAFTSSKM KHMYQLICE CAERFTQHF GNRTVDVEI KDVFSRFSN DVIASAAFG IQVDSLKEP GNEFYSMGK AVTSILNGF QMVKVFLAL SFPNIAKLI QFRAIPPKV TSFFYDIII DNIKKRKNE GIVRPDMVH LLMEAQSGK LKHDNNTEQ VDGFATVEE SEIGKNSNK IQLTDELIV AQALIFLLA GFDSVSTGL SFIAYELAT NPDVQKKLQ EEIDSVLEK NQGKDKSTI IAILKQFVK MLTLILGVI LGAIIYYKL RKPAHFWAE KNIPHLSSW PIVGNMWPF VVQKKHITE IIADIYRQF PTDRYVGYY HFMTPTLLL RDVELIKQI GVKEFDSFH DHVSVSNTN TEPLLSEAL TNLPGEKWK EMRATLSPA FTSSKIKNM YSLISECAE NFVDFFQGR EQDVEIKEV FSRFSNDVI ATAAFGIRV NSVQEPNNE FYAMGRSLT TFSAFQILK IFLAHLVPK IAEFFEYRV LPRKVTDFF EGLILDNMQ KREAEKIVR PDLIHLLME ARKGKLKHD NSNEGGEGF ATVEESEIG KNTKQVELT DEKIVAQAL LFFFAGFET VSTGVSFMA YELATNPHV QKKLQKEID LTLQENHGK ISYNVLQSM KYLDQVVCE SLRLWPPAP QTDRLCNKN FVIEASKPH ERTFTVEKD TMVMISMFA IHRDPQYFP DPEKFDPER FSDENKAKI VPGTYMPFG VGPRNCIAK FDIVPNGKT VIPIKISPS TANMVPQEG IHLTFKPRL
- >TC034188 RB
MLTIILSVV LGALIYYKL IKPASFWEE RNITHASAW PLIGSMWES VIQKKNFNE ISADIYKKY PNDRYVGYM QFSNPALLI RDLNLIKQI GVKAFDHFH DHLAFVGVK AEPLFSKSL AILQGEEWK QMRATLSPV FTSSKMKNM YHLIYECAE NFAKHFQGR KEDVEVKDI FSKFTNDVI ATAAFGIQI NSLEEPNNE FYLMGKLLT TFKGWQLIK FFFAQSVPR IFELTKLNI FNQKVIKYF YDIIIDNIH KREKEGIIR PDLIHLLME ARKGKLKHD NSNEGGDGF ATVEESDIG KNSKQLELT EDLMVAQAL IFFFAGFET ISTSFSFTA YELATNPDV QKKLQKEID SAFQENHGK ISYNVLQSM KYLDQVVSE SLRLWPPAP QTDRFCNTD FVLEPTKPD ERRFTIEKG VTTIIPIYG IHRDPQYFP NPDKFDPER FSDENKAKI VPGTYMPFG VGPRNCIGS RLALLELKT LFFHLLSKC DIVTNSKTV IPLKISPNN GNITPKEGI HLSFKPRKI NGKIFRRLI ISPLTKMLT TILLTLLSA LIYFKLIKP ASFWETRNV PHVTSWPLV GSMFPVLTQ KKHFVEISQ NIYTQFPNA KCVGYVLFT KPTLLIRDL DLLKQIGVK EFDSFHDHL PFVTEATEP LLSKSLLNL LGDEWRKMR ATLSPAFTS SKMKHMYQL ICECAERFT QHFGNRTVD VEIKDVFSR FSNDVIASA AFGIQVDSL KEPGNEFYS MGKAVTSIL NGFQMVKVF LALSFPNIA KLIQFRAIP PKVTSFFYD IIIDNIKKR KNEGIVRPD MVHLLMEAQ SGKLKHDNN TEQVDGFAT VEESEIGKN SNKIQLTDE LIVAQALIF LLAGFDSVS TGLSFIAYE LATNPDVQK KLQEEIDSV LEKNQGKDK STIIAILKQ FVKMLTLIL GVILGAIIY YKLRKPAHF WAEKNIPHL SSWPIVGNM WPFVVQKKH ITEIIADIY RQFPTDRYV GYYHFMTPT LLLRDVELI KQIGVKEFD SFHDHVSVS NTNTEPLLS EALTNLPGE KWKEMRATL SPAFTSSKI KNMYSLISE CAENFVDFF QGREQDVEI KEVFSRFSN DVIATAAFG IRVNSVQEP NNEFYAMGR SLTTFSAFQ ILKIFLAHL VPKIAEFFE YRVLPRKVT DFFEGLILD NMQKREAEK IVRPDLIHL LMEARKGKL KHDNSNEGG EGFATVEES EIGKNTKQV ELTDEKIVA QALLFFFAG FETVSTGVS FMAYELATN PHVQKKLQK EIDLTLQEN HGKISYNVL QSMKYLDQV VCESLRLWP PAPQTDRLC NKNFVIEAS KPHERTFTV EKDTMVMIS MFAIHRDPQ YFPDPEKFD PERFSDENK AKIVPGTYM PFGVGPRNC IAKFDIVPN GKTVIPIKI SPSTANMVP QEGIHLTFK PRL
- >TC034188 RA
atgttgact ataatccta agtgtggtt ttgggtgcg cttatttat tacaaatta ataaaaccg gccagtttt tgggaagaa agaaacatt actcacgct tcagcatgg cctctgatt ggaagtatg tgggaatcg gttattcaa aagaaaaat ttcaatgaa atatctgcc gatatttac aagaaatac ccaaacgac agatacgtg ggttacatg caattttca aacccagct ctcttaatc agagatttg aatttgatt aaacaaatc ggagtaaaa gcttttgat cattttcac gaccatctt gcgtttgtt ggtgtcaaa gcagagccc ttgttcagt aaaagtctt gctatttta caaggagag gaatggaag caaatgaga gcgacttta agcccagtt ttcactagc agtaaaatg aaaaatatg tatcatttg atttacgaa tgtgccgaa aatttcgcc aaacatttt caaggcagg aaagaagat gtcgaagtg aaggatatt ttttcgaaa tttaccaac gatgtgatc gctacagcg gcattcggg attcaaatt aattcacta gaggagcct aataacgag ttttatttg atgggaaaa ttgctgact acgttcaaa ggttggcaa ctaattaag ttttttttt gcccagtca gttccccga atttttgag ctcactaaa ttaaacatt tttaatcaa aaagttata aaatatttt tatgatata attattgac aatatacac aaaagggaa aaagaggga attattcgt cccgatttg attcattta ttgatggaa gctcgtaaa ggaaaactt aaacatgat aatagtaat gaaggtggt gatggtttt gccacagtt gaagaatcc gacattggc aaaaactcc aaacagttg gaactaaca gaagactta atggttgct caagctttg attttcttt ttcgctggt tttgaaacg atttcgacc agtttcagt ttcacggct tatgaattg gccacaaat cctgacgtt cagaagaaa ctacaaaag gaaatagac tcggcattt caagaaaat cacggaaaa atttcatac aatgtgtta caatcaatg aaatatctg gaccaagtt gtgtccggt gggtatttc cactgtctt aaatttttt tacgatttt tttgcagaa agtttaaga ctttggcca ccagctccc caaaccgat cgtttctgc aacaccgac tttgttctt gaaccaaca aaaccagac gaaagacga tttacgatc gagaaaggt gttacaaca attattccg atttatgga atacataga gaccctcag tactttcca aatccggat aaattcgat cctgaacgg tttagtgac gaaaataag gccaaaatt gtgccaggg acttatatg ccgtttggt gtcggtcca agaaattgc attggttcg aggcttgct ttgttggaa ctgaaaaca ctattcttc cacttgtta tcaaaatgt gatattgtg acgaacagc aagacggta attccgctt aaaatcagc ccaaataat gggaatatt acgccaaaa gagggaatc cacctttcg ttcaaaccc agaaaaata aacgggaaa atcttccgc cgactcatc atcagtcca ttaaccaaa atgttaacg accattttg ctcacgctt ctctccgct ttaatttac ttcaagtta ataaaaccg gcaagtttt tgggaaacg cgcaacgtc ccccacgtc acatcatgg cctctagtt ggaagcatg ttccctgtg ctaacccaa aagaaacac tttgtggaa atttcccaa aacatttac acccaattt ccgaacgcc aaatgcgtg ggttacgtc ctattcaca aaacccacc cttttgatc cgcgacttg gacctcctt aagcaaatt ggcgtcaaa gaattcgac agtttccac gaccatttg cccttcgtg accgaagcc acggagcct ctcttgagc aaaagcctc cttaattta ctgggggat gagtggagg aaaatgcgg gcgactcta agcccggcc ttcaccagc agcaaaatg aaacacatg taccaatta atttgcgag tgtgccgaa cgtttcacc caacatttc gggaaccgg actgttgat gtggaaatc aaggacgtg ttttcacgg tttagcaac gatgtgatt gcaagtgcg gcgttcgga atccaagtc gattcgtta aaagagcca ggaaatgag ttctattca atgggcaaa gctgtgact tcgatactt aacggcttt caaatggtc aaagttttc ctggctttg tccttcccc aacattgct aaacttata caatttaga gcaatcccg cccaaagtc acaagtttt ttttacgac ataataata gataatata aaaaaacgc aaaaatgag ggaattgtt cgcccagac atggtccac cttttgatg gaagcccaa agtgggaaa ctaaaacat gacaacaac acggaacaa gttgatggg tttgcaaca gttgaggaa tcggaaatt ggcaaaaac tccaacaaa atccagctc acagacgaa ctaatcgtg gcacaagcc cttattttt ttactagct ggtttcgac agtgtttcc accggttta agcttcata gcgtacgaa ttagccact aatcctgac gttcagaag aaactacaa gaagaaata gactcggtg cttgagaaa aatcaaggg aaagataaa tcaacaatt attgcaata ttgaaacag ttcgtaaaa atgttgact ctaatattg ggtgtaatt ttgggtgcg ataatttac tacaagttg agaaaaccg gcccatttt tgggccgaa aaaaacatc ccccacctg tcatcatgg cccattgtt ggcaacatg tggccattt gtggtccaa aagaaacac atcacggaa ataattgct gatatttac cgccagttt ccgacagac agatatgtg ggttattat cacttcatg accccgact ctcttgcta cgagacgtt gaattgatt aaacaaatc ggagtgaag gaatttgat agttttcac gaccacgtt tctgtcagt aataccaac actgaacct ttactcagt gaagctttg actaattta cctggagag aagtggaag gaaatgagg gcgactcta agtccggct tttaccagc agtaaaatc aaaaatatg tacagtttg atctccgaa tgtgcggaa aatttcgtc gattttttc caaggcagg gagcaggac gttgaaatc aaggaagtt ttctcacgg tttagcaac gatgtgatt gcaactgcg gcctttggt attcgagtt aactcggtc caggaaccc aacaatgaa ttttatgcc atggggcga tcgctaacc actttcagt gccttccaa attttgaaa atattcctg gcacatctt gtaccaaaa atcgctgaa ttttttgaa tatagggta ctaccaagg aaagtcaca gattttttc gaaggttta attttggac aatatgcag aaacgggag gctgaaaaa attgttcgg cccgatttg attcattta ttgatggaa gctcgtaaa ggaaaactt aaacatgat aatagtaat gaaggtggt gagggtttt gccacagtt gaagaatca gaaattggc aaaaacacg aaacaagtg gaactaact gatgagaaa attgtggcc caagctctg ctctttttt ttcgctggt tttgaaact gtttccacg ggtgtcagc ttcatggcg tatgaattg gccacaaac ccacacgtt cagaagaaa ctacaaaag gaaatagac ttgacactg caagaaaat cacggaaaa atttcgtac aacgtgcta caatcgatg aaatatctg gaccaagtt gtgtgtgaa agtttacga ctttggcca ccggctcca caaaccgac cgtttgtgt aataaaaat tttgtaatt gaagcgagc aaaccccat gagaggact ttcacagtg gagaaggat acaatggtt atgatatcg atgtttgca attcatcgg gatcctcaa tattttccg gatcctgaa aaatttgat cctgaacgg tttagtgac gaaaataag gcgaaaatt gtgccaggg acgtacatg ccgtttggt gtgggacca cgaaattgc atcgcaaaa ttcgatatt gtaccaaat ggaaagaca gttattccg attaaaatt agcccaagt acggccaat atggtgcca caagagggc atccacctc acgttcaaa cctaggttg tga
- >TC034188 RB
atgttgact ataatccta agtgtggtt ttgggtgcg cttatttat tacaaatta ataaaaccg gccagtttt tgggaagaa agaaacatt actcacgct tcagcatgg cctctgatt ggaagtatg tgggaatcg gttattcaa aagaaaaat ttcaatgaa atatctgcc gatatttac aagaaatac ccaaacgac agatacgtg ggttacatg caattttca aacccagct ctcttaatc agagatttg aatttgatt aaacaaatc ggagtaaaa gcttttgat cattttcac gaccatctt gcgtttgtt ggtgtcaaa gcagagccc ttgttcagt aaaagtctt gctatttta caaggagag gaatggaag caaatgaga gcgacttta agcccagtt ttcactagc agtaaaatg aaaaatatg tatcatttg atttacgaa tgtgccgaa aatttcgcc aaacatttt caaggcagg aaagaagat gtcgaagtg aaggatatt ttttcgaaa tttaccaac gatgtgatc gctacagcg gcattcggg attcaaatt aattcacta gaggagcct aataacgag ttttatttg atgggaaaa ttgctgact acgttcaaa ggttggcaa ctaattaag ttttttttt gcccagtca gttccccga atttttgag ctcactaaa ttaaacatt tttaatcaa aaagttata aaatatttt tatgatata attattgac aatatacac aaaagggaa aaagaggga attattcgt cccgatttg attcattta ttgatggaa gctcgtaaa ggaaaactt aaacatgat aatagtaat gaaggtggt gatggtttt gccacagtt gaagaatcc gacattggc aaaaactcc aaacagttg gaactaaca gaagactta atggttgct caagctttg attttcttt ttcgctggt tttgaaacg atttcgacc agtttcagt ttcacggct tatgaattg gccacaaat cctgacgtt cagaagaaa ctacaaaag gaaatagac tcggcattt caagaaaat cacggaaaa atttcatac aatgtgtta caatcaatg aaatatctg gaccaagtt gtgtccgaa agtttaaga ctttggcca ccagctccc caaaccgat cgtttctgc aacaccgac tttgttctt gaaccaaca aaaccagac gaaagacga tttacgatc gagaaaggt gttacaaca attattccg atttatgga atacataga gaccctcag tactttcca aatccggat aaattcgat cctgaacgg tttagtgac gaaaataag gccaaaatt gtgccaggg acttatatg ccgtttggt gtcggtcca agaaattgc attggttcg aggcttgct ttgttggaa ctgaaaaca ctattcttc cacttgtta tcaaaatgt gatattgtg acgaacagc aagacggta attccgctt aaaatcagc ccaaataat gggaatatt acgccaaaa gagggaatc cacctttcg ttcaaaccc agaaaaata aacgggaaa atcttccgc cgactcatc atcagtcca ttaaccaaa atgttaacg accattttg ctcacgctt ctctccgct ttaatttac ttcaagtta ataaaaccg gcaagtttt tgggaaacg cgcaacgtc ccccacgtc acatcatgg cctctagtt ggaagcatg ttccctgtg ctaacccaa aagaaacac tttgtggaa atttcccaa aacatttac acccaattt ccgaacgcc aaatgcgtg ggttacgtc ctattcaca aaacccacc cttttgatc cgcgacttg gacctcctt aagcaaatt ggcgtcaaa gaattcgac agtttccac gaccatttg cccttcgtg accgaagcc acggagcct ctcttgagc aaaagcctc cttaattta ctgggggat gagtggagg aaaatgcgg gcgactcta agcccggcc ttcaccagc agcaaaatg aaacacatg taccaatta atttgcgag tgtgccgaa cgtttcacc caacatttc gggaaccgg actgttgat gtggaaatc aaggacgtg ttttcacgg tttagcaac gatgtgatt gcaagtgcg gcgttcgga atccaagtc gattcgtta aaagagcca ggaaatgag ttctattca atgggcaaa gctgtgact tcgatactt aacggcttt caaatggtc aaagttttc ctggctttg tccttcccc aacattgct aaacttata caatttaga gcaatcccg cccaaagtc acaagtttt ttttacgac ataataata gataatata aaaaaacgc aaaaatgag ggaattgtt cgcccagac atggtccac cttttgatg gaagcccaa agtgggaaa ctaaaacat gacaacaac acggaacaa gttgatggg tttgcaaca gttgaggaa tcggaaatt ggcaaaaac tccaacaaa atccagctc acagacgaa ctaatcgtg gcacaagcc cttattttt ttactagct ggtttcgac agtgtttcc accggttta agcttcata gcgtacgaa ttagccact aatcctgac gttcagaag aaactacaa gaagaaata gactcggtg cttgagaaa aatcaaggg aaagataaa tcaacaatt attgcaata ttgaaacag ttcgtaaaa atgttgact ctaatattg ggtgtaatt ttgggtgcg ataatttac tacaagttg agaaaaccg gcccatttt tgggccgaa aaaaacatc ccccacctg tcatcatgg cccattgtt ggcaacatg tggccattt gtggtccaa aagaaacac atcacggaa ataattgct gatatttac cgccagttt ccgacagac agatatgtg ggttattat cacttcatg accccgact ctcttgcta cgagacgtt gaattgatt aaacaaatc ggagtgaag gaatttgat agttttcac gaccacgtt tctgtcagt aataccaac actgaacct ttactcagt gaagctttg actaattta cctggagag aagtggaag gaaatgagg gcgactcta agtccggct tttaccagc agtaaaatc aaaaatatg tacagtttg atctccgaa tgtgcggaa aatttcgtc gattttttc caaggcagg gagcaggac gttgaaatc aaggaagtt ttctcacgg tttagcaac gatgtgatt gcaactgcg gcctttggt attcgagtt aactcggtc caggaaccc aacaatgaa ttttatgcc atggggcga tcgctaacc actttcagt gccttccaa attttgaaa atattcctg gcacatctt gtaccaaaa atcgctgaa ttttttgaa tatagggta ctaccaagg aaagtcaca gattttttc gaaggttta attttggac aatatgcag aaacgggag gctgaaaaa attgttcgg cccgatttg attcattta ttgatggaa gctcgtaaa ggaaaactt aaacatgat aatagtaat gaaggtggt gagggtttt gccacagtt gaagaatca gaaattggc aaaaacacg aaacaagtg gaactaact gatgagaaa attgtggcc caagctctg ctctttttt ttcgctggt tttgaaact gtttccacg ggtgtcagc ttcatggcg tatgaattg gccacaaac ccacacgtt cagaagaaa ctacaaaag gaaatagac ttgacactg caagaaaat cacggaaaa atttcgtac aacgtgcta caatcgatg aaatatctg gaccaagtt gtgtgtgaa agtttacga ctttggcca ccggctcca caaaccgac cgtttgtgt aataaaaat tttgtaatt gaagcgagc aaaccccat gagaggact ttcacagtg gagaaggat acaatggtt atgatatcg atgtttgca attcatcgg gatcctcaa tattttccg gatcctgaa aaatttgat cctgaacgg tttagtgac gaaaataag gcgaaaatt gtgccaggg acgtacatg ccgtttggt gtgggacca cgaaattgc atcgcaaaa ttcgatatt gtaccaaat ggaaagaca gttattccg attaaaatt agcccaagt acggccaat atggtgcca caagagggc atccacctc acgttcaaa cctaggttg tga
- >TC034188 RA
tgtaccaaa atgttgact ataatccta agtgtggtt ttgggtgcg cttatttat tacaaatta ataaaaccg gccagtttt tgggaagaa agaaacatt actcacgct tcagcatgg cctctgatt ggaagtatg tgggaatcg gttattcaa aagaaaaat ttcaatgaa atatctgcc gatatttac aagaaatac ccaaacgac agatacgtg ggttacatg caattttca aacccagct ctcttaatc agagatttg aatttgatt aaacaaatc ggagtaaaa gcttttgat cattttcac gaccatctt gcgtttgtt ggtgtcaaa gcagagccc ttgttcagt aaaagtctt gctatttta caaggagag gaatggaag caaatgaga gcgacttta agcccagtt ttcactagc agtaaaatg aaaaatatg tatcatttg atttacgaa tgtgccgaa aatttcgcc aaacatttt caaggcagg aaagaagat gtcgaagtg aaggatatt ttttcgaaa tttaccaac gatgtgatc gctacagcg gcattcggg attcaaatt aattcacta gaggagcct aataacgag ttttatttg atgggaaaa ttgctgact acgttcaaa ggttggcaa ctaattaag ttttttttt gcccagtca gttccccga atttttgag ctcactaaa ttaaacatt tttaatcaa aaagttata aaatatttt tatgatata attattgac aatatacac aaaagggaa aaagaggga attattcgt cccgatttg attcattta ttgatggaa gctcgtaaa ggaaaactt aaacatgat aatagtaat gaaggtggt gatggtttt gccacagtt gaagaatcc gacattggc aaaaactcc aaacagttg gaactaaca gaagactta atggttgct caagctttg attttcttt ttcgctggt tttgaaacg atttcgacc agtttcagt ttcacggct tatgaattg gccacaaat cctgacgtt cagaagaaa ctacaaaag gaaatagac tcggcattt caagaaaat cacggaaaa atttcatac aatgtgtta caatcaatg aaatatctg gaccaagtt gtgtccggt gggtatttc cactgtctt aaatttttt tacgatttt tttgcagaa agtttaaga ctttggcca ccagctccc caaaccgat cgtttctgc aacaccgac tttgttctt gaaccaaca aaaccagac gaaagacga tttacgatc gagaaaggt gttacaaca attattccg atttatgga atacataga gaccctcag tactttcca aatccggat aaattcgat cctgaacgg tttagtgac gaaaataag gccaaaatt gtgccaggg acttatatg ccgtttggt gtcggtcca agaaattgc attggttcg aggcttgct ttgttggaa ctgaaaaca ctattcttc cacttgtta tcaaaatgt gatattgtg acgaacagc aagacggta attccgctt aaaatcagc ccaaataat gggaatatt acgccaaaa gagggaatc cacctttcg ttcaaaccc agaaaaata aacgggaaa atcttccgc cgactcatc atcagtcca ttaaccaaa atgttaacg accattttg ctcacgctt ctctccgct ttaatttac ttcaagtta ataaaaccg gcaagtttt tgggaaacg cgcaacgtc ccccacgtc acatcatgg cctctagtt ggaagcatg ttccctgtg ctaacccaa aagaaacac tttgtggaa atttcccaa aacatttac acccaattt ccgaacgcc aaatgcgtg ggttacgtc ctattcaca aaacccacc cttttgatc cgcgacttg gacctcctt aagcaaatt ggcgtcaaa gaattcgac agtttccac gaccatttg cccttcgtg accgaagcc acggagcct ctcttgagc aaaagcctc cttaattta ctgggggat gagtggagg aaaatgcgg gcgactcta agcccggcc ttcaccagc agcaaaatg aaacacatg taccaatta atttgcgag tgtgccgaa cgtttcacc caacatttc gggaaccgg actgttgat gtggaaatc aaggacgtg ttttcacgg tttagcaac gatgtgatt gcaagtgcg gcgttcgga atccaagtc gattcgtta aaagagcca ggaaatgag ttctattca atgggcaaa gctgtgact tcgatactt aacggcttt caaatggtc aaagttttc ctggctttg tccttcccc aacattgct aaacttata caatttaga gcaatcccg cccaaagtc acaagtttt ttttacgac ataataata gataatata aaaaaacgc aaaaatgag ggaattgtt cgcccagac atggtccac cttttgatg gaagcccaa agtgggaaa ctaaaacat gacaacaac acggaacaa gttgatggg tttgcaaca gttgaggaa tcggaaatt ggcaaaaac tccaacaaa atccagctc acagacgaa ctaatcgtg gcacaagcc cttattttt ttactagct ggtttcgac agtgtttcc accggttta agcttcata gcgtacgaa ttagccact aatcctgac gttcagaag aaactacaa gaagaaata gactcggtg cttgagaaa aatcaaggg aaagataaa tcaacaatt attgcaata ttgaaacag ttcgtaaaa atgttgact ctaatattg ggtgtaatt ttgggtgcg ataatttac tacaagttg agaaaaccg gcccatttt tgggccgaa aaaaacatc ccccacctg tcatcatgg cccattgtt ggcaacatg tggccattt gtggtccaa aagaaacac atcacggaa ataattgct gatatttac cgccagttt ccgacagac agatatgtg ggttattat cacttcatg accccgact ctcttgcta cgagacgtt gaattgatt aaacaaatc ggagtgaag gaatttgat agttttcac gaccacgtt tctgtcagt aataccaac actgaacct ttactcagt gaagctttg actaattta cctggagag aagtggaag gaaatgagg gcgactcta agtccggct tttaccagc agtaaaatc aaaaatatg tacagtttg atctccgaa tgtgcggaa aatttcgtc gattttttc caaggcagg gagcaggac gttgaaatc aaggaagtt ttctcacgg tttagcaac gatgtgatt gcaactgcg gcctttggt attcgagtt aactcggtc caggaaccc aacaatgaa ttttatgcc atggggcga tcgctaacc actttcagt gccttccaa attttgaaa atattcctg gcacatctt gtaccaaaa atcgctgaa ttttttgaa tatagggta ctaccaagg aaagtcaca gattttttc gaaggttta attttggac aatatgcag aaacgggag gctgaaaaa attgttcgg cccgatttg attcattta ttgatggaa gctcgtaaa ggaaaactt aaacatgat aatagtaat gaaggtggt gagggtttt gccacagtt gaagaatca gaaattggc aaaaacacg aaacaagtg gaactaact gatgagaaa attgtggcc caagctctg ctctttttt ttcgctggt tttgaaact gtttccacg ggtgtcagc ttcatggcg tatgaattg gccacaaac ccacacgtt cagaagaaa ctacaaaag gaaatagac ttgacactg caagaaaat cacggaaaa atttcgtac aacgtgcta caatcgatg aaatatctg gaccaagtt gtgtgtgaa agtttacga ctttggcca ccggctcca caaaccgac cgtttgtgt aataaaaat tttgtaatt gaagcgagc aaaccccat gagaggact ttcacagtg gagaaggat acaatggtt atgatatcg atgtttgca attcatcgg gatcctcaa tattttccg gatcctgaa aaatttgat cctgaacgg tttagtgac gaaaataag gcgaaaatt gtgccaggg acgtacatg ccgtttggt gtgggacca cgaaattgc atcgcaaaa ttcgatatt gtaccaaat ggaaagaca gttattccg attaaaatt agcccaagt acggccaat atggtgcca caagagggc atccacctc acgttcaaa cctaggttg tgaaatata ttatttaaa gtttc
- >TC034188 RB
tgtaccaaa atgttgact ataatccta agtgtggtt ttgggtgcg cttatttat tacaaatta ataaaaccg gccagtttt tgggaagaa agaaacatt actcacgct tcagcatgg cctctgatt ggaagtatg tgggaatcg gttattcaa aagaaaaat ttcaatgaa atatctgcc gatatttac aagaaatac ccaaacgac agatacgtg ggttacatg caattttca aacccagct ctcttaatc agagatttg aatttgatt aaacaaatc ggagtaaaa gcttttgat cattttcac gaccatctt gcgtttgtt ggtgtcaaa gcagagccc ttgttcagt aaaagtctt gctatttta caaggagag gaatggaag caaatgaga gcgacttta agcccagtt ttcactagc agtaaaatg aaaaatatg tatcatttg atttacgaa tgtgccgaa aatttcgcc aaacatttt caaggcagg aaagaagat gtcgaagtg aaggatatt ttttcgaaa tttaccaac gatgtgatc gctacagcg gcattcggg attcaaatt aattcacta gaggagcct aataacgag ttttatttg atgggaaaa ttgctgact acgttcaaa ggttggcaa ctaattaag ttttttttt gcccagtca gttccccga atttttgag ctcactaaa ttaaacatt tttaatcaa aaagttata aaatatttt tatgatata attattgac aatatacac aaaagggaa aaagaggga attattcgt cccgatttg attcattta ttgatggaa gctcgtaaa ggaaaactt aaacatgat aatagtaat gaaggtggt gatggtttt gccacagtt gaagaatcc gacattggc aaaaactcc aaacagttg gaactaaca gaagactta atggttgct caagctttg attttcttt ttcgctggt tttgaaacg atttcgacc agtttcagt ttcacggct tatgaattg gccacaaat cctgacgtt cagaagaaa ctacaaaag gaaatagac tcggcattt caagaaaat cacggaaaa atttcatac aatgtgtta caatcaatg aaatatctg gaccaagtt gtgtccgaa agtttaaga ctttggcca ccagctccc caaaccgat cgtttctgc aacaccgac tttgttctt gaaccaaca aaaccagac gaaagacga tttacgatc gagaaaggt gttacaaca attattccg atttatgga atacataga gaccctcag tactttcca aatccggat aaattcgat cctgaacgg tttagtgac gaaaataag gccaaaatt gtgccaggg acttatatg ccgtttggt gtcggtcca agaaattgc attggttcg aggcttgct ttgttggaa ctgaaaaca ctattcttc cacttgtta tcaaaatgt gatattgtg acgaacagc aagacggta attccgctt aaaatcagc ccaaataat gggaatatt acgccaaaa gagggaatc cacctttcg ttcaaaccc agaaaaata aacgggaaa atcttccgc cgactcatc atcagtcca ttaaccaaa atgttaacg accattttg ctcacgctt ctctccgct ttaatttac ttcaagtta ataaaaccg gcaagtttt tgggaaacg cgcaacgtc ccccacgtc acatcatgg cctctagtt ggaagcatg ttccctgtg ctaacccaa aagaaacac tttgtggaa atttcccaa aacatttac acccaattt ccgaacgcc aaatgcgtg ggttacgtc ctattcaca aaacccacc cttttgatc cgcgacttg gacctcctt aagcaaatt ggcgtcaaa gaattcgac agtttccac gaccatttg cccttcgtg accgaagcc acggagcct ctcttgagc aaaagcctc cttaattta ctgggggat gagtggagg aaaatgcgg gcgactcta agcccggcc ttcaccagc agcaaaatg aaacacatg taccaatta atttgcgag tgtgccgaa cgtttcacc caacatttc gggaaccgg actgttgat gtggaaatc aaggacgtg ttttcacgg tttagcaac gatgtgatt gcaagtgcg gcgttcgga atccaagtc gattcgtta aaagagcca ggaaatgag ttctattca atgggcaaa gctgtgact tcgatactt aacggcttt caaatggtc aaagttttc ctggctttg tccttcccc aacattgct aaacttata caatttaga gcaatcccg cccaaagtc acaagtttt ttttacgac ataataata gataatata aaaaaacgc aaaaatgag ggaattgtt cgcccagac atggtccac cttttgatg gaagcccaa agtgggaaa ctaaaacat gacaacaac acggaacaa gttgatggg tttgcaaca gttgaggaa tcggaaatt ggcaaaaac tccaacaaa atccagctc acagacgaa ctaatcgtg gcacaagcc cttattttt ttactagct ggtttcgac agtgtttcc accggttta agcttcata gcgtacgaa ttagccact aatcctgac gttcagaag aaactacaa gaagaaata gactcggtg cttgagaaa aatcaaggg aaagataaa tcaacaatt attgcaata ttgaaacag ttcgtaaaa atgttgact ctaatattg ggtgtaatt ttgggtgcg ataatttac tacaagttg agaaaaccg gcccatttt tgggccgaa aaaaacatc ccccacctg tcatcatgg cccattgtt ggcaacatg tggccattt gtggtccaa aagaaacac atcacggaa ataattgct gatatttac cgccagttt ccgacagac agatatgtg ggttattat cacttcatg accccgact ctcttgcta cgagacgtt gaattgatt aaacaaatc ggagtgaag gaatttgat agttttcac gaccacgtt tctgtcagt aataccaac actgaacct ttactcagt gaagctttg actaattta cctggagag aagtggaag gaaatgagg gcgactcta agtccggct tttaccagc agtaaaatc aaaaatatg tacagtttg atctccgaa tgtgcggaa aatttcgtc gattttttc caaggcagg gagcaggac gttgaaatc aaggaagtt ttctcacgg tttagcaac gatgtgatt gcaactgcg gcctttggt attcgagtt aactcggtc caggaaccc aacaatgaa ttttatgcc atggggcga tcgctaacc actttcagt gccttccaa attttgaaa atattcctg gcacatctt gtaccaaaa atcgctgaa ttttttgaa tatagggta ctaccaagg aaagtcaca gattttttc gaaggttta attttggac aatatgcag aaacgggag gctgaaaaa attgttcgg cccgatttg attcattta ttgatggaa gctcgtaaa ggaaaactt aaacatgat aatagtaat gaaggtggt gagggtttt gccacagtt gaagaatca gaaattggc aaaaacacg aaacaagtg gaactaact gatgagaaa attgtggcc caagctctg ctctttttt ttcgctggt tttgaaact gtttccacg ggtgtcagc ttcatggcg tatgaattg gccacaaac ccacacgtt cagaagaaa ctacaaaag gaaatagac ttgacactg caagaaaat cacggaaaa atttcgtac aacgtgcta caatcgatg aaatatctg gaccaagtt gtgtgtgaa agtttacga ctttggcca ccggctcca caaaccgac cgtttgtgt aataaaaat tttgtaatt gaagcgagc aaaccccat gagaggact ttcacagtg gagaaggat acaatggtt atgatatcg atgtttgca attcatcgg gatcctcaa tattttccg gatcctgaa aaatttgat cctgaacgg tttagtgac gaaaataag gcgaaaatt gtgccaggg acgtacatg ccgtttggt gtgggacca cgaaattgc atcgcaaaa ttcgatatt gtaccaaat ggaaagaca gttattccg attaaaatt agcccaagt acggccaat atggtgcca caagagggc atccacctc acgttcaaa cctaggttg tgaaatata ttatttaaa gtttc
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