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Gene information
General information
TC005339 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14334211..14338678
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005339
    iBeetle screen iB_00831

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005339 RA
      MDIVESVSK RTFSGSSGT YNVQALELA AARERSKLK 
      GFNITVRFL DDTEHEFHV GKRCKGSVI MDQVYQHLE 
      LVEKDYFGL QYSENGSAP NVNKPELMR WLDPSKPIK 
      KQLNNCQYP LYFRVKFYV SDPSKLQEE YTRYQFCLQ 
      IRRDILEGR LVLAPSTAI LLASYTVQA ELGDYQPEE 
      HGPNYLSSI QLIPGQCEE MEKKIAELH KLHKGQSPA 
      QAEFNFLDH AKRIEMYGV ELHKTRDNT NREIQLGVT 
      HMGLVVFQN NIKINTFSW SKIMKISFK RKQFFIQLI 
      RELSEDYDT LLGFNMESY RSSKTLWKA CVEHHTFFR 
      LDSPKVKRK FPFSLGSKF TYSGRTEFQ TVADVKRRG 
      KLERKFVRS PSKQLVRPV PPIEEKGKG ILAPGRPPR 
      PYDNKVTSL GAKEPRKAW ADDSQLSDD DGGFLERTL 
      EGPFSPGGR VLSYVDEEI PSPTSNGLY DTPPYSASH 
      SPTSQITDE TLVTITLHP EEGKYGFNV KGGTSRDVP 
      ILVSRVAPN TPADKCTPR LSEGDQVIQ INGVDVSHA 
      LHEDVVMLI HRARGTSDG KLVLVVKPN VLYAGMEEF 
      EEPPYQYVP VGELQRPPS PENALEQSM LLLADGLAS 
      GALIARYEM LYRKHPELT CDVSLESKN INKNRYRDI 
      SPYDSTRVI LKNAPSGDY INANYVNMK INGTDIINK 
      YIATQGPLQ STCEDFWQM ILEEKCNLI VMLTTLIER 
      GRAKCHKYW PNVGETLTM QNVIIQCVG ETTDESGSF 
      IFRDFSLTD VKNNTEWNL KHIQYVAWP DHGVPDSPA 
      LFLSFTEKV REARKGDAP VVVHCSAGI GRTGVLVLM 
      ETALCLIEA GEPVYPLEI VKTMREQRA MMIQNASQY 
      RFVCESVHT AYLQTIGDE AKLFR
    CDS sequence
    • >TC005339 RA
      atggacata gtcgagagc gtgtcaaaa cggacgttt 
      agtggctcg agtggtact tataatgtc caagcttta 
      gaactggca gcagcacgg gaacggagc aaactgaag 
      ggctttaac atcacggtg cgttttctc gacgacact 
      gagcatgag ttccatgtg ggcaaaagg tgcaaaggc 
      agcgttatc atggaccaa gtgtaccag cacttggag 
      ctcgttgag aaggactac ttcgggctc cagtactcg 
      gaaaatggg agtgctccc aatgtcaat aaaccggaa 
      ctcatgcgt tggttagat ccgtccaaa cccatcaaa 
      aagcagctg aacaactgc caataccct ttgtatttt 
      agagttaag ttttatgtg tctgatcca agtaagctg 
      caagaggag tacacgcga taccagttt tgtctacaa 
      atccgccga gatattcta gaagggagg ctggtttta 
      gcccccagc actgccatt cttttagct agttataca 
      gttcaagcc gagttaggg gattatcaa cccgaggaa 
      catgggccc aattattta tccagcata caactcata 
      ccaggacaa tgcgaggaa atggagaag aaaattgca 
      gaattacat aaattacac aaaggacaa tctccagct 
      caagctgaa tttaatttt ctcgatcat gcaaaacgc 
      atcgaaatg tacggtgta gagctccat aaaactcga 
      gataacacc aaccgggaa attcagtta ggtgtgacc 
      catatgggt ctggtcgtc tttcaaaac aacataaaa 
      atcaacaca ttttcatgg tcgaaaata atgaaaatt 
      tcattcaaa cgtaaacag tttttcatc cagttgatc 
      cgcgaactg tccgaagat tacgatact ttgctcgga 
      ttcaacatg gaatcgtat cgttcgtca aaaacactc 
      tggaaagct tgtgttgaa catcacact ttctttcga 
      ttggattca ccaaaagtt aaacgaaaa ttccctttt 
      tcgttgggc tcgaaattt acttattct ggtcggact 
      gaatttcaa acagttgcc gatgtcaaa cgaagagga 
      aaactcgaa cggaaattt gttcgatcg ccgagtaaa 
      caattggtt cgtccagtg ccaccaatt gaagaaaag 
      ggcaaaggg atactagcc ccaggtcgc cctccgaga 
      ccttacgat aacaaagtc acttctttg ggggccaaa 
      gaaccgagg aaagcatgg gctgatgat tcacagttg 
      tctgatgac gatggtggt tttctggaa cgtactcta 
      gaaggtcca ttttccccc ggtggccgt gttttaagt 
      tacgttgat gaggaaatt ccatcgcct acttcaaac 
      ggtttgtat gatacacct ccatacagc gctagccat 
      tcgccaacg tcacaaatc accgatgaa actctagta 
      actattact ttacatccc gaggaggga aaatacggt 
      tttaacgtt aagggtggt acaagtcgt gatgtcccg 
      attttggtg tctagagta gcaccaaat acaccagct 
      gataaatgc acacctcgt ttatctgaa ggtgatcaa 
      gtgattcag ataaatggg gtcgacgtt tcgcatgct 
      ttacatgaa gatgttgtt atgttaata cacagggcg 
      aggggtaca agtgatgga aaattggta ttggtggtt 
      aaacccaac gttttgtat gccggtatg gaagaattc 
      gaagaaccc ccataccaa tacgtccct gtaggtgaa 
      ttacaacgc cccccatct ccggaaaat gcccttgag 
      caaagcatg ctcctattg gctgatggt ttagcgagt 
      ggcgccctc atcgcccgc tatgaaatg ttatatcgc 
      aaacatccc gaattaaca tgcgatgtt tcacttgaa 
      tcgaaaaat atcaacaag aatcgttac agggatatt 
      tcgccttat gactcgact cgtgtgata ctaaaaaat 
      gcacccagt ggtgattac atcaacgcc aactacgtc 
      aatatgaaa ataaacggt actgatata attaataag 
      tatatagcg actcaaggc ccactacaa tcaacctgt 
      gaggacttt tggcaaatg attttggaa gagaaatgt 
      aatttaatt gtgatgtta acgacacta attgaacgc 
      ggacgtgcc aagtgtcat aaatattgg ccaaatgtg 
      ggcgaaact ttgacgatg caaaatgtg ataattcaa 
      tgtgttgga gaaacaacg gacgaatct ggaagtttt 
      attttcaga gattttagt ctgactgac gttaaaaat 
      aacactgaa tggaacttg aaacacatc cagtatgtg 
      gcatggcca gatcatgga gtacccgat tctcccgca 
      ctttttctc agttttacg gaaaaagtg cgagaggcc 
      cgtaaaggg gatgcccct gttgtggtc cactgtagt 
      gcagggatt ggtcgaact ggtgtcctg gttctcatg 
      gagacggcc ttgtgctta attgaggcc ggggaacca 
      gtgtacccc cttgaaatt gtcaaaacg atgagagag 
      caacgagca atgatgata caaaatgct agtcaatat 
      cggttcgtt tgcgaaagt gttcacaca gcttatttg 
      caaacaatt ggtgacgaa gcgaagctt tttaggtga
    mRNA sequence
    • >TC005339 RA
      aatctgggc aaaaaccag ccattgacg tatcaataa 
      tcaaataat gaatgaaat actaaaatt gtgataaaa 
      ccactttgt gataacact aacgtgaat gtgatgcat 
      cgaattcgt gccaaaccc tttgaaatt gcgtgagcc 
      ctttgcgac agctgatcg aaatcgcag ctaaccatg 
      atgatatga cttgagtgt caaaccgaa aaaagcgct 
      tcactgtct tatttcctt taatttggt cgttatcac 
      ttatcagtg ggctagttc tatgtttgt gtcgggtcc 
      gtaatgccc caaagcgca gttcctagc catggacat 
      agtcgagag cgtgtcaaa acggacgtt tagtggctc 
      gagtggtac ttataatgt ccaagcttt agaactggc 
      agcagcacg ggaacggag caaactgaa gggctttaa 
      catcacggt gcgttttct cgacgacac tgagcatga 
      gttccatgt gggcaaaag gtgcaaagg cagcgttat 
      catggacca agtgtacca gcacttgga gctcgttga 
      gaaggacta cttcgggct ccagtactc ggaaaatgg 
      gagtgctcc caatgtcaa taaaccgga actcatgcg 
      ttggttaga tccgtccaa acccatcaa aaagcagct 
      gaacaactg ccaataccc tttgtattt tagagttaa 
      gttttatgt gtctgatcc aagtaagct gcaagagga 
      gtacacgcg ataccagtt ttgtctaca aatccgccg 
      agatattct agaagggag gctggtttt agcccccag 
      cactgccat tcttttagc tagttatac agttcaagc 
      cgagttagg ggattatca acccgagga acatgggcc 
      caattattt atccagcat acaactcat accaggaca 
      atgcgagga aatggagaa gaaaattgc agaattaca 
      taaattaca caaaggaca atctccagc tcaagctga 
      atttaattt tctcgatca tgcaaaacg catcgaaat 
      gtacggtgt agagctcca taaaactcg agataacac 
      caaccggga aattcagtt aggtgtgac ccatatggg 
      tctggtcgt ctttcaaaa caacataaa aatcaacac 
      attttcatg gtcgaaaat aatgaaaat ttcattcaa 
      acgtaaaca gtttttcat ccagttgat ccgcgaact 
      gtccgaaga ttacgatac tttgctcgg attcaacat 
      ggaatcgta tcgttcgtc aaaaacact ctggaaagc 
      ttgtgttga acatcacac tttctttcg attggattc 
      accaaaagt taaacgaaa attcccttt ttcgttggg 
      ctcgaaatt tacttattc tggtcggac tgaatttca 
      aacagttgc cgatgtcaa acgaagagg aaaactcga 
      acggaaatt tgttcgatc gccgagtaa acaattggt 
      tcgtccagt gccaccaat tgaagaaaa gggcaaagg 
      gatactagc cccaggtcg ccctccgag accttacga 
      taacaaagt cacttcttt gggggccaa agaaccgag 
      gaaagcatg ggctgatga ttcacagtt gtctgatga 
      cgatggtgg ttttctgga acgtactct agaaggtcc 
      attttcccc cggtggccg tgttttaag ttacgttga 
      tgaggaaat tccatcgcc tacttcaaa cggtttgta 
      tgatacacc tccatacag cgctagcca ttcgccaac 
      gtcacaaat caccgatga aactctagt aactattac 
      tttacatcc cgaggaggg aaaatacgg ttttaacgt 
      taagggtgg tacaagtcg tgatgtccc gattttggt 
      gtctagagt agcaccaaa tacaccagc tgataaatg 
      cacacctcg tttatctga aggtgatca agtgattca 
      gataaatgg ggtcgacgt ttcgcatgc tttacatga 
      agatgttgt tatgttaat acacagggc gaggggtac 
      aagtgatgg aaaattggt attggtggt taaacccaa 
      cgttttgta tgccggtat ggaagaatt cgaagaacc 
      cccatacca atacgtccc tgtaggtga attacaacg 
      ccccccatc tccggaaaa tgcccttga gcaaagcat 
      gctcctatt ggctgatgg tttagcgag tggcgccct 
      catcgcccg ctatgaaat gttatatcg caaacatcc 
      cgaattaac atgcgatgt ttcacttga atcgaaaaa 
      tatcaacaa gaatcgtta cagggatat ttcgcctta 
      tgactcgac tcgtgtgat actaaaaaa tgcacccag 
      tggtgatta catcaacgc caactacgt caatatgaa 
      aataaacgg tactgatat aattaataa gtatatagc 
      gactcaagg cccactaca atcaacctg tgaggactt 
      ttggcaaat gattttgga agagaaatg taatttaat 
      tgtgatgtt aacgacact aattgaacg cggacgtgc 
      caagtgtca taaatattg gccaaatgt gggcgaaac 
      tttgacgat gcaaaatgt gataattca atgtgttgg 
      agaaacaac ggacgaatc tggaagttt tattttcag 
      agattttag tctgactga cgttaaaaa taacactga 
      atggaactt gaaacacat ccagtatgt ggcatggcc 
      agatcatgg agtacccga ttctcccgc actttttct 
      cagttttac ggaaaaagt gcgagaggc ccgtaaagg 
      ggatgcccc tgttgtggt ccactgtag tgcagggat 
      tggtcgaac tggtgtcct ggttctcat ggagacggc 
      cttgtgctt aattgaggc cggggaacc agtgtaccc 
      ccttgaaat tgtcaaaac gatgagaga gcaacgagc 
      aatgatgat acaaaatgc tagtcaata tcggttcgt 
      ttgcgaaag tgttcacac agcttattt gcaaacaat 
      tggtgacga agcgaagct ttttaggtg agaaggttt 
      tgtttagga cagccaggg tgttcaact aatccgtcg 
      tgaggtaat gtgattgtt tttcattga accggcggt 
      gccttgagg caattattt ttttcatta acaaatact 
      cacatcacg aatttgatt cgctgcagt ttgattttt 
      ccttgtttt cggggattg ttagtgctt tagcacagc 
      ctacttgat ggataggga cacttaatt aattatgtg 
      acgctcact atcgaagag gctgtggct tgtggttat 
      taggcagtt attaatcgt actaagatg aagacatta 
      aaggctgat tttttctat gtaaattaa tattgtccc 
      gtattagat gaattgtaa aggatagaa aaatatttg 
      aagagtgtt cgtgttcat cactttaaa aactgtgtt 
      ttccttttg tttaacaaa ctaattacg atttttttc 
      taaaaaaaa agtacaaaa agcacttgt at
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