General information | |||
TC034193
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 14345244..14356002 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC034193 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00830 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
- >TC034193 RA
MCCSHTTGS CRSVCEKIS LAQLAADSR LRNETVEEV RKFCSPQLS PFWECLNAT FKDMSRGES WSGRVCCPI PQSNSCRKA CITATSTQD LAQGCRQSD EIEFFRCLE RQKSGEECC SNARSDDCH QVCLEIFRS RWTPNPPLR LKVQEMCEA NSPKVMDCV KDFIKVTPA KDLHKKKHC CDMSNQSKC RETCKKLLS MKNITVQES IDGLQLGGC GSPLPQEYF WSCFLKSTE TAETTVEVS RIERVGMDS AKSHCCPRA ASHSCQKLC TKTFTKFWS TSWDEFYNR CLTQVMEEN LRNCIDEVD EPCELGCDG LSFCTNFNN RPTELFRSC NTQADEAAR NDVSVWQFQ NKLTLPGLI LPLKNISQC SPNVWKTVA CTLQIKPCS RHSHANQIC RDVCLDILS KCVDWTRIS PEHSAETIC ASLSPEDPN VSCIRLQNF LYPSETSVQ RISEQVFSP CKGNPCEPN EVCLLNRKC IHGTNCLPY KCVPGCKLG EVSEYKVPH GTYVWIPND NNNKQQSCL KICKCNAGK IEECQPLLC VKLKPCLMG TAMHGTSFN IDCKTCSCF ASEDICSKK QCESSALTG ENTAYTTLP CNCVPHYVP VCGKDGNNY PSACLAKCA GLTDSEIEP APCEDPCRS HTCPIGHKC VPRPQICLF SREHRDCKQ YECINGTTN CHNLPKSPV CSTNNTEFD NSCLLAHHN AKLAYHGPC LRNCRHEGV VCGFNGRTY ISECAAWAD MVSVDYVGR CRRVGLIGT TKTKRCPDV KCQALPDPN CLGVTPPGA CCPICGGTL NLLYSRQQI NRALYALDT NTDSLTLKA MLKALDRQI QVAQCVLRG YLTVEMDIF VTVQTTEKY PSKLQLEAC VQEAEKIAS LVNMQSPRI VSEVTLSSL TLANVIHVN SASSLGATM VFVVLLLRL F
- >TC034193 RA
atgtgctgt tcgcatacc accggctcc tgcagaagt gtatgcgag aagatatct ttggctcaa ttggcggcc gatagtcgt ctaaggaac gaaactgtg gaagaagta cggaaattc tgttcacct cagctgagt ccgttctgg gagtgcctc aatgcaacg ttcaaagat atgtcgcgt ggagagtcg tggtcaggc cgcgtctgc tgccccatc ccccaaagc aacagctgc cgaaaagct tgcattact gcaacctcc actcaagac ctggcccaa ggttgccgc caaagcgac gaaatcgaa tttttccgc tgcttagaa cgccaaaaa agcggagaa gagtgttgc agtaatgcg agaagtgac gattgccac caggtgtgt cttgaaatc tttaggagt cggtggacg cctaatccg ccactacga ctcaaagtc caggaaatg tgcgaagcc aacagtccc aaagtcatg gactgtgtc aaggatttc attaaagtg acgccggca aaggattta cacaaaaag aagcactgt tgcgacatg tccaaccag agcaagtgt cgcgaaact tgcaaaaaa ctactctct atgaaaaat atcacggtc caagagagc atagatggg ttacagttg ggtgggtgt gggtcacct ctcccccag gagtacttc tggtcctgt ttcctcaaa tcgaccgaa actgctgaa accacagtc gaagtttca cgaatcgaa agggttggg atggattcg gcaaaatcc cactgttgt ccaagggcc gcaagtcac tcgtgccaa aaactgtgc actaagaca ttcaccaag ttttggtca acgtcctgg gacgagttt tacaatcgg tgtttgacg caagtcatg gaggagaac ttaaggaat tgtatcgat gaagtggac gaaccatgc gagcttgga tgcgatggt cttagtttt tgtacaaat ttcaacaac cgaccgact gagttgttc cgttcttgt aacactcaa gcagacgaa gcggcccgg aatgacgtt tccgtgtgg caatttcag aacaaatta acactccct ggacttatc ctcccactt aaaaacatc tctcaatgc tcgccaaat gtctggaag actgtggcc tgcactctc cagattaaa ccctgttcg aggcacagt catgccaat caaatttgt cgcgacgtt tgcctggat attctctcg aaatgtgtg gattggacc cgaatatca cctgaacat tcggctgaa acgatctgt gctagttta tcacccgaa gatccgaac gtttcctgc atccgtctt caaaatttc ctatatcca agtgagaca agtgtccag agaatcagc gaacaggtt ttttcgccg tgtaaaggc aacccatgt gagcccaac gaagtgtgc cttttaaac cgaaaatgc atccatggg accaattgt ctcccgtac aagtgtgtc cctgggtgc aaactaggc gaagtgagc gagtacaaa gtaccacac ggtacctat gtctggatc cccaatgac aataacaac aaacaacaa agttgcttg aagatttgt aaatgcaac gcggggaaa atcgaagaa tgccaacct ctcctgtgc gtcaaactc aaaccatgt ctcatgggc accgccatg catggtact tcatttaat atcgactgc aagacgtgt agttgtttt gcgagtgag gatatttgt agcaagaaa caatgtgaa agtagtgcc ctgacgggc gaaaacaca gcttacacc acactgcct tgtaattgt gtcccacat tacgtacca gtgtgtggg aaagatggt aataattac ccatcggcc tgtttagcc aagtgcgcc ggtttgacc gactctgag attgaacca gccccatgt gaggaccca tgccgctcc cacacctgt cccatcggc cacaagtgc gtcccaaga ccccaaatt tgtctcttc tcgcgcgag catcgcgat tgcaaacaa tacgagtgc attaatggt accaccaat tgccacaac ctgcccaaa tcccccgtc tgcagtacc aacaacacc gaattcgac aactcctgt cttttggcg caccataac gccaaactg gcctaccat ggcccctgt ttgcgcaac tgccgccac gaaggagtc gtttgtggt ttcaatggt cgcacctac atctcagaa tgtgcagca tgggctgac atggtctcc gttgattac gttgggcgt tgtagaaga gtcggttta attggaacc accaaaacc aagcgatgt cccgacgtg aaatgccag gctttgccc gaccccaat tgtctcgga gtgacccca ccgggggct tgctgcccc atttgtggc ggtaccctt aacttgctc tactcccgc cagcaaata aaccgagct ttgtatgcc ctggacaca aacactgat tccctgact ttgaaagcg atgctcaaa gctctggat cggcaaatt caagtcgcg caatgtgta cttcgaggg tatttgact gtggaaatg gatattttc gtgacggtg caaacgaca gagaagtac ccgtccaag ttgcagctc gaggcttgt gtgcaagag gcggagaag atcgcaagt ttggtcaat atgcaaagt ccgaggatt gtgagtgaa gttacgttg agttcgctc actttggct aatgttatc catgtgaat tcggcgtcg agtttgggg gcaacgatg gtttttgtg gttttgtta ttgagattg ttttga
- >TC034193 RA
agtcgtcga aatgagtgt gtgatcgtt tcttcagca agcgcgcgt tcacgtgca acaccatgt catttcaga ctaataaaa gtgccattt tttcccttt gctttattg tgctttaca cccggatat tagcgcaag atttaatgt gctgttcgc ataccaccg gctcctgca gaagtgtat gcgagaaga tatctttgg ctcaattgg cggccgata gtcgtctaa ggaacgaaa ctgtggaag aagtacgga aattctgtt cacctcagc tgagtccgt tctgggagt gcctcaatg caacgttca aagatatgt cgcgtggag agtcgtggt caggccgcg tctgctgcc ccatccccc aaagcaaca gctgccgaa aagcttgca ttactgcaa cctccactc aagacctgg cccaaggtt gccgccaaa gcgacgaaa tcgaatttt tccgctgct tagaacgcc aaaaaagcg gagaagagt gttgcagta atgcgagaa gtgacgatt gccaccagg tgtgtcttg aaatcttta ggagtcggt ggacgccta atccgccac tacgactca aagtccagg aaatgtgcg aagccaaca gtcccaaag tcatggact gtgtcaagg atttcatta aagtgacgc cggcaaagg atttacaca aaaagaagc actgttgcg acatgtcca accagagca agtgtcgcg aaacttgca aaaaactac tctctatga aaaatatca cggtccaag agagcatag atgggttac agttgggtg ggtgtgggt cacctctcc cccaggagt acttctggt cctgtttcc tcaaatcga ccgaaactg ctgaaacca cagtcgaag tttcacgaa tcgaaaggg ttgggatgg attcggcaa aatcccact gttgtccaa gggccgcaa gtcactcgt gccaaaaac tgtgcacta agacattca ccaagtttt ggtcaacgt cctgggacg agttttaca atcggtgtt tgacgcaag tcatggagg agaacttaa ggaattgta tcgatgaag tggacgaac catgcgagc ttggatgcg atggtctta gtttttgta caaatttca acaaccgac cgactgagt tgttccgtt cttgtaaca ctcaagcag acgaagcgg cccggaatg acgtttccg tgtggcaat ttcagaaca aattaacac tccctggac ttatcctcc cacttaaaa acatctctc aatgctcgc caaatgtct ggaagactg tggcctgca ctctccaga ttaaaccct gttcgaggc acagtcatg ccaatcaaa tttgtcgcg acgtttgcc tggatattc tctcgaaat gtgtggatt ggacccgaa tatcacctg aacattcgg ctgaaacga tctgtgcta gtttatcac ccgaagatc cgaacgttt cctgcatcc gtcttcaaa atttcctat atccaagtg agacaagtg tccagagaa tcagcgaac aggtttttt cgccgtgta aaggcaacc catgtgagc ccaacgaag tgtgccttt taaaccgaa aatgcatcc atgggacca attgtctcc cgtacaagt gtgtccctg ggtgcaaac taggcgaag tgagcgagt acaaagtac cacacggta cctatgtct ggatcccca atgacaata acaacaaac aacaaagtt gcttgaaga tttgtaaat gcaacgcgg ggaaaatcg aagaatgcc aacctctcc tgtgcgtca aactcaaac catgtctca tgggcaccg ccatgcatg gtacttcat ttaatatcg actgcaaga cgtgtagtt gttttgcga gtgaggata tttgtagca agaaacaat gtgaaagta gtgccctga cgggcgaaa acacagctt acaccacac tgccttgta attgtgtcc cacattacg taccagtgt gtgggaaag atggtaata attacccat cggcctgtt tagccaagt gcgccggtt tgaccgact ctgagattg aaccagccc catgtgagg acccatgcc gctcccaca cctgtccca tcggccaca agtgcgtcc caagacccc aaatttgtc tcttctcgc gcgagcatc gcgattgca aacaatacg agtgcatta atggtacca ccaattgcc acaacctgc ccaaatccc ccgtctgca gtaccaaca acaccgaat tcgacaact cctgtcttt tggcgcacc ataacgcca aactggcct accatggcc cctgtttgc gcaactgcc gccacgaag gagtcgttt gtggtttca atggtcgca cctacatct cagaatgtg cagcatggg ctgacatgg tctccgttg attacgttg ggcgttgta gaagagtcg gtttaattg gaaccacca aaaccaagc gatgtcccg acgtgaaat gccaggctt tgcccgacc ccaattgtc tcggagtga ccccaccgg gggcttgct gccccattt gtggcggta cccttaact tgctctact cccgccagc aaataaacc gagctttgt atgccctgg acacaaaca ctgattccc tgactttga aagcgatgc tcaaagctc tggatcggc aaattcaag tcgcgcaat gtgtacttc gagggtatt tgactgtgg aaatggata ttttcgtga cggtgcaaa cgacagaga agtacccgt ccaagttgc agctcgagg cttgtgtgc aagaggcgg agaagatcg caagtttgg tcaatatgc aaagtccga ggattgtga gtgaagtta cgttgagtt cgctcactt tggctaatg ttatccatg tgaattcgg cgtcgagtt tgggggcaa cgatggttt ttgtggttt tgttattga gattgtttt gagctataa gtaggttct accaaaaac tttgatctc gcggaaaag aggccaaat attatatac caaagaata tgcgactgc ggttgtgcc atcgctcta ttttcggcg ctttgacaa ttacgcttc tatttttag agcgttaca aaggaactt ggatcgttg ggaaaatta tgcaaagac ttacacaca agtgccaaa ttgcgttgt tagggtggg ccagtttct agcaagtct gtgataatt attatgtac gatattgtg taaataact gatacttta cgcggttta ttaacagag ctgtaaata acaaatgta aatagaaga gatgatttt ataccgctt tgtttattt taacaagtt taagactcg acaatcagt tatagttaa gttttatct gtcgtaatt tgattttat ttcttgtgt aattttata accaaatta ttttgtaat ttattgaaa tacattatt aaaaaaact
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
