Status
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Gene information
General information
TC034193
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005336,TC005335 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14345244..14356002
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034193
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00830

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034193 RA
      MCCSHTTGS CRSVCEKIS LAQLAADSR LRNETVEEV 
RKFCSPQLS PFWECLNAT FKDMSRGES WSGRVCCPI 
PQSNSCRKA CITATSTQD LAQGCRQSD EIEFFRCLE 
RQKSGEECC SNARSDDCH QVCLEIFRS RWTPNPPLR 
LKVQEMCEA NSPKVMDCV KDFIKVTPA KDLHKKKHC 
CDMSNQSKC RETCKKLLS MKNITVQES IDGLQLGGC 
GSPLPQEYF WSCFLKSTE TAETTVEVS RIERVGMDS 
AKSHCCPRA ASHSCQKLC TKTFTKFWS TSWDEFYNR 
CLTQVMEEN LRNCIDEVD EPCELGCDG LSFCTNFNN 
RPTELFRSC NTQADEAAR NDVSVWQFQ NKLTLPGLI 
LPLKNISQC SPNVWKTVA CTLQIKPCS RHSHANQIC 
RDVCLDILS KCVDWTRIS PEHSAETIC ASLSPEDPN 
VSCIRLQNF LYPSETSVQ RISEQVFSP CKGNPCEPN 
EVCLLNRKC IHGTNCLPY KCVPGCKLG EVSEYKVPH 
GTYVWIPND NNNKQQSCL KICKCNAGK IEECQPLLC 
VKLKPCLMG TAMHGTSFN IDCKTCSCF ASEDICSKK 
QCESSALTG ENTAYTTLP CNCVPHYVP VCGKDGNNY 
PSACLAKCA GLTDSEIEP APCEDPCRS HTCPIGHKC 
VPRPQICLF SREHRDCKQ YECINGTTN CHNLPKSPV 
CSTNNTEFD NSCLLAHHN AKLAYHGPC LRNCRHEGV 
VCGFNGRTY ISECAAWAD MVSVDYVGR CRRVGLIGT 
TKTKRCPDV KCQALPDPN CLGVTPPGA CCPICGGTL 
NLLYSRQQI NRALYALDT NTDSLTLKA MLKALDRQI 
QVAQCVLRG YLTVEMDIF VTVQTTEKY PSKLQLEAC 
VQEAEKIAS LVNMQSPRI VSEVTLSSL TLANVIHVN 
SASSLGATM VFVVLLLRL F
    CDS sequence
    • >TC034193 RA
      atgtgctgt tcgcatacc accggctcc tgcagaagt 
gtatgcgag aagatatct ttggctcaa ttggcggcc 
gatagtcgt ctaaggaac gaaactgtg gaagaagta 
cggaaattc tgttcacct cagctgagt ccgttctgg 
gagtgcctc aatgcaacg ttcaaagat atgtcgcgt 
ggagagtcg tggtcaggc cgcgtctgc tgccccatc 
ccccaaagc aacagctgc cgaaaagct tgcattact 
gcaacctcc actcaagac ctggcccaa ggttgccgc 
caaagcgac gaaatcgaa tttttccgc tgcttagaa 
cgccaaaaa agcggagaa gagtgttgc agtaatgcg 
agaagtgac gattgccac caggtgtgt cttgaaatc 
tttaggagt cggtggacg cctaatccg ccactacga 
ctcaaagtc caggaaatg tgcgaagcc aacagtccc 
aaagtcatg gactgtgtc aaggatttc attaaagtg 
acgccggca aaggattta cacaaaaag aagcactgt 
tgcgacatg tccaaccag agcaagtgt cgcgaaact 
tgcaaaaaa ctactctct atgaaaaat atcacggtc 
caagagagc atagatggg ttacagttg ggtgggtgt 
gggtcacct ctcccccag gagtacttc tggtcctgt 
ttcctcaaa tcgaccgaa actgctgaa accacagtc 
gaagtttca cgaatcgaa agggttggg atggattcg 
gcaaaatcc cactgttgt ccaagggcc gcaagtcac 
tcgtgccaa aaactgtgc actaagaca ttcaccaag 
ttttggtca acgtcctgg gacgagttt tacaatcgg 
tgtttgacg caagtcatg gaggagaac ttaaggaat 
tgtatcgat gaagtggac gaaccatgc gagcttgga 
tgcgatggt cttagtttt tgtacaaat ttcaacaac 
cgaccgact gagttgttc cgttcttgt aacactcaa 
gcagacgaa gcggcccgg aatgacgtt tccgtgtgg 
caatttcag aacaaatta acactccct ggacttatc 
ctcccactt aaaaacatc tctcaatgc tcgccaaat 
gtctggaag actgtggcc tgcactctc cagattaaa 
ccctgttcg aggcacagt catgccaat caaatttgt 
cgcgacgtt tgcctggat attctctcg aaatgtgtg 
gattggacc cgaatatca cctgaacat tcggctgaa 
acgatctgt gctagttta tcacccgaa gatccgaac 
gtttcctgc atccgtctt caaaatttc ctatatcca 
agtgagaca agtgtccag agaatcagc gaacaggtt 
ttttcgccg tgtaaaggc aacccatgt gagcccaac 
gaagtgtgc cttttaaac cgaaaatgc atccatggg 
accaattgt ctcccgtac aagtgtgtc cctgggtgc 
aaactaggc gaagtgagc gagtacaaa gtaccacac 
ggtacctat gtctggatc cccaatgac aataacaac 
aaacaacaa agttgcttg aagatttgt aaatgcaac 
gcggggaaa atcgaagaa tgccaacct ctcctgtgc 
gtcaaactc aaaccatgt ctcatgggc accgccatg 
catggtact tcatttaat atcgactgc aagacgtgt 
agttgtttt gcgagtgag gatatttgt agcaagaaa 
caatgtgaa agtagtgcc ctgacgggc gaaaacaca 
gcttacacc acactgcct tgtaattgt gtcccacat 
tacgtacca gtgtgtggg aaagatggt aataattac 
ccatcggcc tgtttagcc aagtgcgcc ggtttgacc 
gactctgag attgaacca gccccatgt gaggaccca 
tgccgctcc cacacctgt cccatcggc cacaagtgc 
gtcccaaga ccccaaatt tgtctcttc tcgcgcgag 
catcgcgat tgcaaacaa tacgagtgc attaatggt 
accaccaat tgccacaac ctgcccaaa tcccccgtc 
tgcagtacc aacaacacc gaattcgac aactcctgt 
cttttggcg caccataac gccaaactg gcctaccat 
ggcccctgt ttgcgcaac tgccgccac gaaggagtc 
gtttgtggt ttcaatggt cgcacctac atctcagaa 
tgtgcagca tgggctgac atggtctcc gttgattac 
gttgggcgt tgtagaaga gtcggttta attggaacc 
accaaaacc aagcgatgt cccgacgtg aaatgccag 
gctttgccc gaccccaat tgtctcgga gtgacccca 
ccgggggct tgctgcccc atttgtggc ggtaccctt 
aacttgctc tactcccgc cagcaaata aaccgagct 
ttgtatgcc ctggacaca aacactgat tccctgact 
ttgaaagcg atgctcaaa gctctggat cggcaaatt 
caagtcgcg caatgtgta cttcgaggg tatttgact 
gtggaaatg gatattttc gtgacggtg caaacgaca 
gagaagtac ccgtccaag ttgcagctc gaggcttgt 
gtgcaagag gcggagaag atcgcaagt ttggtcaat 
atgcaaagt ccgaggatt gtgagtgaa gttacgttg 
agttcgctc actttggct aatgttatc catgtgaat 
tcggcgtcg agtttgggg gcaacgatg gtttttgtg 
gttttgtta ttgagattg ttttga
    mRNA sequence
    • >TC034193 RA
      agtcgtcga aatgagtgt gtgatcgtt tcttcagca 
agcgcgcgt tcacgtgca acaccatgt catttcaga 
ctaataaaa gtgccattt tttcccttt gctttattg 
tgctttaca cccggatat tagcgcaag atttaatgt 
gctgttcgc ataccaccg gctcctgca gaagtgtat 
gcgagaaga tatctttgg ctcaattgg cggccgata 
gtcgtctaa ggaacgaaa ctgtggaag aagtacgga 
aattctgtt cacctcagc tgagtccgt tctgggagt 
gcctcaatg caacgttca aagatatgt cgcgtggag 
agtcgtggt caggccgcg tctgctgcc ccatccccc 
aaagcaaca gctgccgaa aagcttgca ttactgcaa 
cctccactc aagacctgg cccaaggtt gccgccaaa 
gcgacgaaa tcgaatttt tccgctgct tagaacgcc 
aaaaaagcg gagaagagt gttgcagta atgcgagaa 
gtgacgatt gccaccagg tgtgtcttg aaatcttta 
ggagtcggt ggacgccta atccgccac tacgactca 
aagtccagg aaatgtgcg aagccaaca gtcccaaag 
tcatggact gtgtcaagg atttcatta aagtgacgc 
cggcaaagg atttacaca aaaagaagc actgttgcg 
acatgtcca accagagca agtgtcgcg aaacttgca 
aaaaactac tctctatga aaaatatca cggtccaag 
agagcatag atgggttac agttgggtg ggtgtgggt 
cacctctcc cccaggagt acttctggt cctgtttcc 
tcaaatcga ccgaaactg ctgaaacca cagtcgaag 
tttcacgaa tcgaaaggg ttgggatgg attcggcaa 
aatcccact gttgtccaa gggccgcaa gtcactcgt 
gccaaaaac tgtgcacta agacattca ccaagtttt 
ggtcaacgt cctgggacg agttttaca atcggtgtt 
tgacgcaag tcatggagg agaacttaa ggaattgta 
tcgatgaag tggacgaac catgcgagc ttggatgcg 
atggtctta gtttttgta caaatttca acaaccgac 
cgactgagt tgttccgtt cttgtaaca ctcaagcag 
acgaagcgg cccggaatg acgtttccg tgtggcaat 
ttcagaaca aattaacac tccctggac ttatcctcc 
cacttaaaa acatctctc aatgctcgc caaatgtct 
ggaagactg tggcctgca ctctccaga ttaaaccct 
gttcgaggc acagtcatg ccaatcaaa tttgtcgcg 
acgtttgcc tggatattc tctcgaaat gtgtggatt 
ggacccgaa tatcacctg aacattcgg ctgaaacga 
tctgtgcta gtttatcac ccgaagatc cgaacgttt 
cctgcatcc gtcttcaaa atttcctat atccaagtg 
agacaagtg tccagagaa tcagcgaac aggtttttt 
cgccgtgta aaggcaacc catgtgagc ccaacgaag 
tgtgccttt taaaccgaa aatgcatcc atgggacca 
attgtctcc cgtacaagt gtgtccctg ggtgcaaac 
taggcgaag tgagcgagt acaaagtac cacacggta 
cctatgtct ggatcccca atgacaata acaacaaac 
aacaaagtt gcttgaaga tttgtaaat gcaacgcgg 
ggaaaatcg aagaatgcc aacctctcc tgtgcgtca 
aactcaaac catgtctca tgggcaccg ccatgcatg 
gtacttcat ttaatatcg actgcaaga cgtgtagtt 
gttttgcga gtgaggata tttgtagca agaaacaat 
gtgaaagta gtgccctga cgggcgaaa acacagctt 
acaccacac tgccttgta attgtgtcc cacattacg 
taccagtgt gtgggaaag atggtaata attacccat 
cggcctgtt tagccaagt gcgccggtt tgaccgact 
ctgagattg aaccagccc catgtgagg acccatgcc 
gctcccaca cctgtccca tcggccaca agtgcgtcc 
caagacccc aaatttgtc tcttctcgc gcgagcatc 
gcgattgca aacaatacg agtgcatta atggtacca 
ccaattgcc acaacctgc ccaaatccc ccgtctgca 
gtaccaaca acaccgaat tcgacaact cctgtcttt 
tggcgcacc ataacgcca aactggcct accatggcc 
cctgtttgc gcaactgcc gccacgaag gagtcgttt 
gtggtttca atggtcgca cctacatct cagaatgtg 
cagcatggg ctgacatgg tctccgttg attacgttg 
ggcgttgta gaagagtcg gtttaattg gaaccacca 
aaaccaagc gatgtcccg acgtgaaat gccaggctt 
tgcccgacc ccaattgtc tcggagtga ccccaccgg 
gggcttgct gccccattt gtggcggta cccttaact 
tgctctact cccgccagc aaataaacc gagctttgt 
atgccctgg acacaaaca ctgattccc tgactttga 
aagcgatgc tcaaagctc tggatcggc aaattcaag 
tcgcgcaat gtgtacttc gagggtatt tgactgtgg 
aaatggata ttttcgtga cggtgcaaa cgacagaga 
agtacccgt ccaagttgc agctcgagg cttgtgtgc 
aagaggcgg agaagatcg caagtttgg tcaatatgc 
aaagtccga ggattgtga gtgaagtta cgttgagtt 
cgctcactt tggctaatg ttatccatg tgaattcgg 
cgtcgagtt tgggggcaa cgatggttt ttgtggttt 
tgttattga gattgtttt gagctataa gtaggttct 
accaaaaac tttgatctc gcggaaaag aggccaaat 
attatatac caaagaata tgcgactgc ggttgtgcc 
atcgctcta ttttcggcg ctttgacaa ttacgcttc 
tatttttag agcgttaca aaggaactt ggatcgttg 
ggaaaatta tgcaaagac ttacacaca agtgccaaa 
ttgcgttgt tagggtggg ccagtttct agcaagtct 
gtgataatt attatgtac gatattgtg taaataact 
gatacttta cgcggttta ttaacagag ctgtaaata 
acaaatgta aatagaaga gatgatttt ataccgctt 
tgtttattt taacaagtt taagactcg acaatcagt 
tatagttaa gttttatct gtcgtaatt tgattttat 
ttcttgtgt aattttata accaaatta ttttgtaat 
ttattgaaa tacattatt aaaaaaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY