Status
Please wait ...
Gene information
General information
TC034193
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005336,TC005335
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14345244..14356002
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034193
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00830

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034193 RA
      MCCSHTTGS CRSVCEKIS LAQLAADSR LRNETVEEV 
      RKFCSPQLS PFWECLNAT FKDMSRGES WSGRVCCPI 
      PQSNSCRKA CITATSTQD LAQGCRQSD EIEFFRCLE 
      RQKSGEECC SNARSDDCH QVCLEIFRS RWTPNPPLR 
      LKVQEMCEA NSPKVMDCV KDFIKVTPA KDLHKKKHC 
      CDMSNQSKC RETCKKLLS MKNITVQES IDGLQLGGC 
      GSPLPQEYF WSCFLKSTE TAETTVEVS RIERVGMDS 
      AKSHCCPRA ASHSCQKLC TKTFTKFWS TSWDEFYNR 
      CLTQVMEEN LRNCIDEVD EPCELGCDG LSFCTNFNN 
      RPTELFRSC NTQADEAAR NDVSVWQFQ NKLTLPGLI 
      LPLKNISQC SPNVWKTVA CTLQIKPCS RHSHANQIC 
      RDVCLDILS KCVDWTRIS PEHSAETIC ASLSPEDPN 
      VSCIRLQNF LYPSETSVQ RISEQVFSP CKGNPCEPN 
      EVCLLNRKC IHGTNCLPY KCVPGCKLG EVSEYKVPH 
      GTYVWIPND NNNKQQSCL KICKCNAGK IEECQPLLC 
      VKLKPCLMG TAMHGTSFN IDCKTCSCF ASEDICSKK 
      QCESSALTG ENTAYTTLP CNCVPHYVP VCGKDGNNY 
      PSACLAKCA GLTDSEIEP APCEDPCRS HTCPIGHKC 
      VPRPQICLF SREHRDCKQ YECINGTTN CHNLPKSPV 
      CSTNNTEFD NSCLLAHHN AKLAYHGPC LRNCRHEGV 
      VCGFNGRTY ISECAAWAD MVSVDYVGR CRRVGLIGT 
      TKTKRCPDV KCQALPDPN CLGVTPPGA CCPICGGTL 
      NLLYSRQQI NRALYALDT NTDSLTLKA MLKALDRQI 
      QVAQCVLRG YLTVEMDIF VTVQTTEKY PSKLQLEAC 
      VQEAEKIAS LVNMQSPRI VSEVTLSSL TLANVIHVN 
      SASSLGATM VFVVLLLRL F
    CDS sequence
    • >TC034193 RA
      atgtgctgt tcgcatacc accggctcc tgcagaagt 
      gtatgcgag aagatatct ttggctcaa ttggcggcc 
      gatagtcgt ctaaggaac gaaactgtg gaagaagta 
      cggaaattc tgttcacct cagctgagt ccgttctgg 
      gagtgcctc aatgcaacg ttcaaagat atgtcgcgt 
      ggagagtcg tggtcaggc cgcgtctgc tgccccatc 
      ccccaaagc aacagctgc cgaaaagct tgcattact 
      gcaacctcc actcaagac ctggcccaa ggttgccgc 
      caaagcgac gaaatcgaa tttttccgc tgcttagaa 
      cgccaaaaa agcggagaa gagtgttgc agtaatgcg 
      agaagtgac gattgccac caggtgtgt cttgaaatc 
      tttaggagt cggtggacg cctaatccg ccactacga 
      ctcaaagtc caggaaatg tgcgaagcc aacagtccc 
      aaagtcatg gactgtgtc aaggatttc attaaagtg 
      acgccggca aaggattta cacaaaaag aagcactgt 
      tgcgacatg tccaaccag agcaagtgt cgcgaaact 
      tgcaaaaaa ctactctct atgaaaaat atcacggtc 
      caagagagc atagatggg ttacagttg ggtgggtgt 
      gggtcacct ctcccccag gagtacttc tggtcctgt 
      ttcctcaaa tcgaccgaa actgctgaa accacagtc 
      gaagtttca cgaatcgaa agggttggg atggattcg 
      gcaaaatcc cactgttgt ccaagggcc gcaagtcac 
      tcgtgccaa aaactgtgc actaagaca ttcaccaag 
      ttttggtca acgtcctgg gacgagttt tacaatcgg 
      tgtttgacg caagtcatg gaggagaac ttaaggaat 
      tgtatcgat gaagtggac gaaccatgc gagcttgga 
      tgcgatggt cttagtttt tgtacaaat ttcaacaac 
      cgaccgact gagttgttc cgttcttgt aacactcaa 
      gcagacgaa gcggcccgg aatgacgtt tccgtgtgg 
      caatttcag aacaaatta acactccct ggacttatc 
      ctcccactt aaaaacatc tctcaatgc tcgccaaat 
      gtctggaag actgtggcc tgcactctc cagattaaa 
      ccctgttcg aggcacagt catgccaat caaatttgt 
      cgcgacgtt tgcctggat attctctcg aaatgtgtg 
      gattggacc cgaatatca cctgaacat tcggctgaa 
      acgatctgt gctagttta tcacccgaa gatccgaac 
      gtttcctgc atccgtctt caaaatttc ctatatcca 
      agtgagaca agtgtccag agaatcagc gaacaggtt 
      ttttcgccg tgtaaaggc aacccatgt gagcccaac 
      gaagtgtgc cttttaaac cgaaaatgc atccatggg 
      accaattgt ctcccgtac aagtgtgtc cctgggtgc 
      aaactaggc gaagtgagc gagtacaaa gtaccacac 
      ggtacctat gtctggatc cccaatgac aataacaac 
      aaacaacaa agttgcttg aagatttgt aaatgcaac 
      gcggggaaa atcgaagaa tgccaacct ctcctgtgc 
      gtcaaactc aaaccatgt ctcatgggc accgccatg 
      catggtact tcatttaat atcgactgc aagacgtgt 
      agttgtttt gcgagtgag gatatttgt agcaagaaa 
      caatgtgaa agtagtgcc ctgacgggc gaaaacaca 
      gcttacacc acactgcct tgtaattgt gtcccacat 
      tacgtacca gtgtgtggg aaagatggt aataattac 
      ccatcggcc tgtttagcc aagtgcgcc ggtttgacc 
      gactctgag attgaacca gccccatgt gaggaccca 
      tgccgctcc cacacctgt cccatcggc cacaagtgc 
      gtcccaaga ccccaaatt tgtctcttc tcgcgcgag 
      catcgcgat tgcaaacaa tacgagtgc attaatggt 
      accaccaat tgccacaac ctgcccaaa tcccccgtc 
      tgcagtacc aacaacacc gaattcgac aactcctgt 
      cttttggcg caccataac gccaaactg gcctaccat 
      ggcccctgt ttgcgcaac tgccgccac gaaggagtc 
      gtttgtggt ttcaatggt cgcacctac atctcagaa 
      tgtgcagca tgggctgac atggtctcc gttgattac 
      gttgggcgt tgtagaaga gtcggttta attggaacc 
      accaaaacc aagcgatgt cccgacgtg aaatgccag 
      gctttgccc gaccccaat tgtctcgga gtgacccca 
      ccgggggct tgctgcccc atttgtggc ggtaccctt 
      aacttgctc tactcccgc cagcaaata aaccgagct 
      ttgtatgcc ctggacaca aacactgat tccctgact 
      ttgaaagcg atgctcaaa gctctggat cggcaaatt 
      caagtcgcg caatgtgta cttcgaggg tatttgact 
      gtggaaatg gatattttc gtgacggtg caaacgaca 
      gagaagtac ccgtccaag ttgcagctc gaggcttgt 
      gtgcaagag gcggagaag atcgcaagt ttggtcaat 
      atgcaaagt ccgaggatt gtgagtgaa gttacgttg 
      agttcgctc actttggct aatgttatc catgtgaat 
      tcggcgtcg agtttgggg gcaacgatg gtttttgtg 
      gttttgtta ttgagattg ttttga
    mRNA sequence
    • >TC034193 RA
      agtcgtcga aatgagtgt gtgatcgtt tcttcagca 
      agcgcgcgt tcacgtgca acaccatgt catttcaga 
      ctaataaaa gtgccattt tttcccttt gctttattg 
      tgctttaca cccggatat tagcgcaag atttaatgt 
      gctgttcgc ataccaccg gctcctgca gaagtgtat 
      gcgagaaga tatctttgg ctcaattgg cggccgata 
      gtcgtctaa ggaacgaaa ctgtggaag aagtacgga 
      aattctgtt cacctcagc tgagtccgt tctgggagt 
      gcctcaatg caacgttca aagatatgt cgcgtggag 
      agtcgtggt caggccgcg tctgctgcc ccatccccc 
      aaagcaaca gctgccgaa aagcttgca ttactgcaa 
      cctccactc aagacctgg cccaaggtt gccgccaaa 
      gcgacgaaa tcgaatttt tccgctgct tagaacgcc 
      aaaaaagcg gagaagagt gttgcagta atgcgagaa 
      gtgacgatt gccaccagg tgtgtcttg aaatcttta 
      ggagtcggt ggacgccta atccgccac tacgactca 
      aagtccagg aaatgtgcg aagccaaca gtcccaaag 
      tcatggact gtgtcaagg atttcatta aagtgacgc 
      cggcaaagg atttacaca aaaagaagc actgttgcg 
      acatgtcca accagagca agtgtcgcg aaacttgca 
      aaaaactac tctctatga aaaatatca cggtccaag 
      agagcatag atgggttac agttgggtg ggtgtgggt 
      cacctctcc cccaggagt acttctggt cctgtttcc 
      tcaaatcga ccgaaactg ctgaaacca cagtcgaag 
      tttcacgaa tcgaaaggg ttgggatgg attcggcaa 
      aatcccact gttgtccaa gggccgcaa gtcactcgt 
      gccaaaaac tgtgcacta agacattca ccaagtttt 
      ggtcaacgt cctgggacg agttttaca atcggtgtt 
      tgacgcaag tcatggagg agaacttaa ggaattgta 
      tcgatgaag tggacgaac catgcgagc ttggatgcg 
      atggtctta gtttttgta caaatttca acaaccgac 
      cgactgagt tgttccgtt cttgtaaca ctcaagcag 
      acgaagcgg cccggaatg acgtttccg tgtggcaat 
      ttcagaaca aattaacac tccctggac ttatcctcc 
      cacttaaaa acatctctc aatgctcgc caaatgtct 
      ggaagactg tggcctgca ctctccaga ttaaaccct 
      gttcgaggc acagtcatg ccaatcaaa tttgtcgcg 
      acgtttgcc tggatattc tctcgaaat gtgtggatt 
      ggacccgaa tatcacctg aacattcgg ctgaaacga 
      tctgtgcta gtttatcac ccgaagatc cgaacgttt 
      cctgcatcc gtcttcaaa atttcctat atccaagtg 
      agacaagtg tccagagaa tcagcgaac aggtttttt 
      cgccgtgta aaggcaacc catgtgagc ccaacgaag 
      tgtgccttt taaaccgaa aatgcatcc atgggacca 
      attgtctcc cgtacaagt gtgtccctg ggtgcaaac 
      taggcgaag tgagcgagt acaaagtac cacacggta 
      cctatgtct ggatcccca atgacaata acaacaaac 
      aacaaagtt gcttgaaga tttgtaaat gcaacgcgg 
      ggaaaatcg aagaatgcc aacctctcc tgtgcgtca 
      aactcaaac catgtctca tgggcaccg ccatgcatg 
      gtacttcat ttaatatcg actgcaaga cgtgtagtt 
      gttttgcga gtgaggata tttgtagca agaaacaat 
      gtgaaagta gtgccctga cgggcgaaa acacagctt 
      acaccacac tgccttgta attgtgtcc cacattacg 
      taccagtgt gtgggaaag atggtaata attacccat 
      cggcctgtt tagccaagt gcgccggtt tgaccgact 
      ctgagattg aaccagccc catgtgagg acccatgcc 
      gctcccaca cctgtccca tcggccaca agtgcgtcc 
      caagacccc aaatttgtc tcttctcgc gcgagcatc 
      gcgattgca aacaatacg agtgcatta atggtacca 
      ccaattgcc acaacctgc ccaaatccc ccgtctgca 
      gtaccaaca acaccgaat tcgacaact cctgtcttt 
      tggcgcacc ataacgcca aactggcct accatggcc 
      cctgtttgc gcaactgcc gccacgaag gagtcgttt 
      gtggtttca atggtcgca cctacatct cagaatgtg 
      cagcatggg ctgacatgg tctccgttg attacgttg 
      ggcgttgta gaagagtcg gtttaattg gaaccacca 
      aaaccaagc gatgtcccg acgtgaaat gccaggctt 
      tgcccgacc ccaattgtc tcggagtga ccccaccgg 
      gggcttgct gccccattt gtggcggta cccttaact 
      tgctctact cccgccagc aaataaacc gagctttgt 
      atgccctgg acacaaaca ctgattccc tgactttga 
      aagcgatgc tcaaagctc tggatcggc aaattcaag 
      tcgcgcaat gtgtacttc gagggtatt tgactgtgg 
      aaatggata ttttcgtga cggtgcaaa cgacagaga 
      agtacccgt ccaagttgc agctcgagg cttgtgtgc 
      aagaggcgg agaagatcg caagtttgg tcaatatgc 
      aaagtccga ggattgtga gtgaagtta cgttgagtt 
      cgctcactt tggctaatg ttatccatg tgaattcgg 
      cgtcgagtt tgggggcaa cgatggttt ttgtggttt 
      tgttattga gattgtttt gagctataa gtaggttct 
      accaaaaac tttgatctc gcggaaaag aggccaaat 
      attatatac caaagaata tgcgactgc ggttgtgcc 
      atcgctcta ttttcggcg ctttgacaa ttacgcttc 
      tatttttag agcgttaca aaggaactt ggatcgttg 
      ggaaaatta tgcaaagac ttacacaca agtgccaaa 
      ttgcgttgt tagggtggg ccagtttct agcaagtct 
      gtgataatt attatgtac gatattgtg taaataact 
      gatacttta cgcggttta ttaacagag ctgtaaata 
      acaaatgta aatagaaga gatgatttt ataccgctt 
      tgtttattt taacaagtt taagactcg acaatcagt 
      tatagttaa gttttatct gtcgtaatt tgattttat 
      ttcttgtgt aattttata accaaatta ttttgtaat 
      ttattgaaa tacattatt aaaaaaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY