Status
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Gene information
General information
TC034198
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005327 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14433168..14451902
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034198
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00829

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 leg musculature tb1-ptar1 not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    leg musculature femural musculature mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    Comment: the muscles in the coxa are present in all cases
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034198 RA
      MNSTLPQND TIMFNYSIL DIGNDTHKN VTGTPHDYI 
PYSKRPETY VVPVLFFVI FVVGVLGNG TLVVIFLRH 
RTMRNVPNT YILSLALAD LLVIVTSVP FTSIIYTLE 
SWPWGELIC KVSETAKDI SIGVSVFTL TALSADRFF 
AIVDPLKKF HTTSGGRKA TRITLCTAI SIWILAILC 
AIPAAIGSH LKDIEGQFI VCYPFPTHW LNHKYPQVM 
ILLRFLILY VIPLTIIAI FYLNMANHL IVSTRNVPG 
EMQGTQRQI RARKKVAIT VLIFVLVFA LCFLPSHIF 
MLFFYFDPN SSEHYNAFW HYLRIIGFC LCYLNSCAN 
PVALYWVSG AFRKHFNRY LLCIKPKRS RCNTCQGHQ 
ATTLSYVPT RKNLSVCSR KSRGLSINR REPVNGQET 
SITLLGNGN GHVCSKM
    CDS sequence
    • >TC034198 RA
      atgaattcc acattaccg cagaatgac acgattatg 
ttcaactac agcatactc gacattggt aatgataca 
cacaagaat gttactgga acacctcat gattacatt 
ccctacagt aaaagaccg gaaacgtac gttgttccg 
gtcttgttc ttcgttatt tttgtggtg ggagtgttg 
ggaaatggg acgcttgtt gtgattttc ctgaggcac 
aggacgatg cgaaatgtt ccaaacaca tacatactt 
tcactggct ttggcagat ctgcttgtt atagtgacg 
agtgtccct ttcacttcg attatatac actttggag 
tcatggcca tggggtgaa ctgatctgc aaggtgtct 
gaaacggcc aaagatatc tccattgga gtctcagta 
ttcacactc actgcatta tctgcagac cgctttttc 
gctattgtg gatcctctc aagaagttt cacaccacg 
agtggagga aggaaagcc actcggatc actctctgt 
acggccatt tcgatatgg attttggcg attttgtgc 
gctattcca gcagcaatt ggatcacac ttgaaggat 
atagaagga cagtttatc gtctgttat ccctttcct 
acacactgg ttgaaccat aaatatccc caagtgatg 
attctattg cgatttctc attttatac gttattcct 
ctaactatt attgcaatt ttctatctg aacatggca 
aaccatttg atcgtcagc actaggaac gttccagga 
gaaatgcaa gggacacaa agacagatt agagctcgc 
aagaaagtc gcaatcacg gtcctcatt ttcgtgctt 
gtgttcgct ttgtgtttc ctgccctcg cacatcttc 
atgctgttc ttctacttt gatccaaac tcttctgaa 
cactacaac gcattctgg cactattta agaataatc 
ggtttctgc ctatgttac ttgaattct tgcgccaat 
ccggtggct ctgtattgg gttagcgga gctttcagg 
aagcatttt aatcgatat cttctctgc atcaaaccg 
aaacgttcc cgctgtaac acttgtcaa gggcaccaa 
gcaaccact ctttcctat gttccaact cggaaaaac 
ctttcggtt tgcagtcgg aaaagccgt ggtttgtcc 
atcaatcga cgagagcca gtcaatgga caagaaact 
tccatcaca cttttaggc aacggaaac ggtcacgtc 
tgtagcaaa atgtaa
    mRNA sequence
    • >TC034198 RA
      caattcata agctttcgg taaatttca gtgtagtac 
gcgcggctt tctgattaa gctcggata tttatgagt 
cattcattc attatcgaa aaaaaactc gcgaactta 
aaaatcgaa agttggacg ttttcggaa tttgcattt 
tggtgcact ccaatggat acaataacg tgatcggtt 
gcattgatc aacaatgaa ttccacatt accgcagaa 
tgacacgat tatgttcaa ctacagcat actcgacat 
tggtaatga tacacacaa gaatgttac tggaacacc 
tcatgatta cattcccta cagtaaaag accggaaac 
gtacgttgt tccggtctt gttcttcgt tatttttgt 
ggtgggagt gttgggaaa tgggacgct tgttgtgat 
tttcctgag gcacaggac gatgcgaaa tgttccaaa 
cacatacat actttcact ggctttggc agatctgct 
tgttatagt gacgagtgt ccctttcac ttcgattat 
atacacttt ggagtcatg gccatgggg tgaactgat 
ctgcaaggt gtctgaaac ggccaaaga tatctccat 
tggagtctc agtattcac actcactgc attatctgc 
agaccgctt tttcgctat tgtggatcc tctcaagaa 
gtttcacac cacgagtgg aggaaggaa agccactcg 
gatcactct ctgtacggc catttcgat atggatttt 
ggcgatttt gtgcgctat tccagcagc aattggatc 
acacttgaa ggatataga aggacagtt tatcgtctg 
ttatccctt tcctacaca ctggttgaa ccataaata 
tccccaagt gatgattct attgcgatt tctcatttt 
atacgttat tcctctaac tattattgc aattttcta 
tctgaacat ggcaaacca tttgatcgt cagcactag 
gaacgttcc aggagaaat gcaagggac acaaagaca 
gattagagc tcgcaagaa agtcgcaat cacggtcct 
cattttcgt gcttgtgtt cgctttgtg tttcctgcc 
ctcgcacat cttcatgct gttcttcta ctttgatcc 
aaactcttc tgaacacta caacgcatt ctggcacta 
tttaagaat aatcggttt ctgcctatg ttacttgaa 
ttcttgcgc caatccggt ggctctgta ttgggttag 
cggagcttt caggaagca ttttaatcg atatcttct 
ctgcatcaa accgaaacg ttcccgctg taacacttg 
tcaagggca ccaagcaac cactctttc ctatgttcc 
aactcggaa aaacctttc ggtttgcag tcggaaaag 
ccgtggttt gtccatcaa tcgacgaga gccagtcaa 
tggacaaga aacttccat cacactttt aggcaacgg 
aaacggtca cgtctgtag caaaatgta agcgatttt 
tccttgata ttaatttcg gaaaagtgt ttgattttt 
tttatataa atcggtgtg tgagagcga acgaatctg 
tacatagtc aatgtttaa taagcataa gggaaaaga 
gtacactta caaaccaaa tgttaatct tgatgtaca 
atatcgatg tttaaataa aacgttttc aaatag

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY