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Gene information
General information
TC005322 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid58: 1304229..1308687
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005322
    iBeetle screen iB_00828
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 5
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (22 dpi)
    Days post injection


    Adult morphology
    motility decreased - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005322 RA
      MALNQALRN FLKSKNRQE PASFIHLFE LYTSLEHDP 
      HEEKALPKF LKELNRKKT EEERLDFMY NVLQKYNLF 
      RNKFTRDRK RDEHAEHCK LEGNVYFSE GNYSFALGF 
      YTKALMGAN GPEMLAICY ANRSAALLK LGLYEECLI 
      DIKRAIDHN YPERLRPKL MSRRVKAVA LKRKQKFES 
      TYYEEAPKI PTSQRNNSI PCATNSVEI KVDEKLRKR 
      VVATKDIQI GQVIAVETP CVAALINVV LFHCHDCYI 
      LCYNPIPCK TCTEVVYCS EACRENAFA KYHQKECPI 
      YLSMRKLVG IDTHFQWAL KMTLLVQTQ ADKCCDRID 
      TDDDRTRKT LMATLFYHL IKNCTTVLA GVDEAGVKN 
      FKRVLMSYM HICDYHVSD IDEIFVHGG SRDLELKQE 
      TFAKAMYPF SDKLRHSCC PNVMGWYHG VTRVLRAIR 
      TIKKGEECF FSYGPLYTN IEKEERQNY IFFIYNFKC 
      ACRACKQNW PQTESVPRV AIEDHMFLY TRKRFDVDA 
      AKMLINRKI DLMKQLERS EPNDELINQ QEMLFHCFY 
      ILGNKRAFQ SQLLACVQD DTRNSGEFV RDFHKLGSN 
      VEKVDFLYE RMRQYGLLD DKFTKDEKD DKKAKKHKE 
      KGDKFFATG AFSKALQSY TRSVMKAKS SEALAMAYA 
      NRSAALFKL EQFEEALTD VERALSGDY PENLRPSLV 
      TRREKLLGF MGAHGPAFN FFESVPEIP EEQKNASIP 
      SASSCIEIK PDEKSRRRV FAARKIEIG EIIAVEKPF 
      VFTLAAADL YHCHECYQL CYNPIPCEI CSQTLYCGE 
      ECRDKAREK YHQYECPIL ISLKNIVGK HKAFLLAIK 
      MSFMISDEN DVPEVYALV ENLSRDNND EVFTTALIT 
      ALMYHLVKT YTGKFPEDD LEAENKFKH FLMTHLRIC 
      LTHAAGIDE LYPNQVSEG QEPGQELLS FKSETVGCA 
      LYPFYALFR HACCPNVFA HHHGTQRVL RAVRTIHEG 
      QECFVSYGP YYVEHSKQE RKSRLLSQY HFTCKCRAC 
      EEDWPQLDL NPYYLTSKQ DLDFVAKIR NANIEAAVE 
      EIPRFLERL KGLEVFEPL SELTLQQKL LSYCFDMLG 
      NKRRIL
    CDS sequence
    • >TC005322 RA
      atggcatta aatcaggct ctaagaaat tttctgaaa 
      tctaaaaat cgacaagag cctgcaagt ttcatccac 
      ctgtttgag ttgtataca agtctcgaa cacgatcct 
      catgaagag aaagccctt cccaagttc ctgaaagaa 
      ttaaaccgg aagaaaact gaagaagag aggcttgat 
      ttcatgtac aacgtgctc cagaagtac aacttgttc 
      agaaataag tttacgcga gatagaaaa cgtgatgaa 
      catgcggaa cattgtaaa ctcgaaggc aacgtttat 
      ttttctgag ggcaactat agctttgct ttggggttc 
      tacacaaag gctcttatg ggtgctaat ggaccagaa 
      atgctggct atatgttac gctaatcgt tcagctgct 
      ttgctcaag ctggggttg tatgaagag tgtctgatt 
      gacattaaa agagctata gatcacaat tatcccgaa 
      cgtttgaga ccgaaatta atgtcgcgg cgtgtgaaa 
      gcagttgca cttaaaaga aaacaaaaa ttcgaaagt 
      acttattat gaggaggct cctaaaatt cccacttct 
      caacgaaac aattcaatt ccttgtgca accaattcg 
      gttgagatt aaagtcgat gagaaattg cgtaaacgt 
      gttgttgcc actaaagac atccaaatt ggacaagtg 
      attgcggta gaaacgcct tgtgttgca gcgttaatt 
      aacgttgtg ctgtttcat tgtcatgac tgttacatt 
      ctttgctac aacccaatt ccttgtaaa acttgcaca 
      gaagtggtc tactgtagt gaagcatgc agggaaaat 
      gccttcgcc aagtaccac cagaaagag tgccccatt 
      tacttatct atgagaaaa ttagtggga attgatact 
      cattttcaa tgggctttg aaaatgact cttcttgtt 
      caaacacaa gcggataag tgttgtgat agaatagat 
      acagatgac gatcgtacc aggaaaaca ttaatggca 
      accctgttt taccatctt atcaagaac tgtacaaca 
      gttttggct ggagttgat gaagctggt gtgaagaat 
      tttaaaagg gttttgatg tcgtacatg cacatttgc 
      gactatcac gtttctgat attgatgag atttttgtt 
      catggggga tctcgtgac ttggaatta aaacaggaa 
      acttttgca aaggccatg tatccgttt tctgacaaa 
      ttgaggcac tcttgttgc cccaatgtt atgggctgg 
      tatcatgga gtgacaaga gtattaagg gccattagg 
      accatcaaa aagggggaa gagtgtttt ttctcttat 
      gggcctctt tatacgaac attgagaaa gaagagcgc 
      caaaattac atctttttt atttacaac tttaaatgc 
      gcatgccgt gcttgtaaa caaaattgg cctcagact 
      gaaagtgtg cctcgagta gcgatcgaa gaccacatg 
      tttctatac acaaggaag agatttgat gtggatgct 
      gctaaaatg cttattaac agaaaaatt gacttgatg 
      aagcaactg gaaaggagt gaaccaaat gatgaattg 
      attaaccag caagaaatg ctgttccac tgcttctac 
      attttggga aataagcga gcgtttcaa agtcagctc 
      ctggcttgt gtgcaagat gacactcgc aattcggga 
      gagtttgtg cgcgatttt cacaagttg ggaagtaat 
      gtagagaaa gtggatttt ttgtatgag aggatgcga 
      cagtacggc ttgcttgac gacaaattt accaaggat 
      gaaaaagat gataagaaa gcgaagaaa cacaaggaa 
      aagggcgat aagtttttt gcaactggg gctttttcc 
      aaagccctt cagtcttat actaggagc gtcatgaag 
      gccaaaagc tcggaagcc ttggctatg gcttacgcc 
      aaccgatct gcagccctg ttcaaactc gaacagttc 
      gaggaggct ctaactgat gtagaaaga gctctttct 
      ggcgactac cctgaaaat ctgaggccc agtttggtg 
      acgcgtcgc gagaaactt ttgggtttt atgggtgcc 
      catggccct gcattcaat ttctttgaa tctgttcct 
      gaaattcca gaggagcag aaaaatgcc tcaattccg 
      agcgctagc tcctgtatt gaaataaaa ccagacgaa 
      aagtcacgt cgccgtgtt ttcgcagct cggaaaatc 
      gaaatcggc gaaataata gcggtagaa aaaccgttt 
      gttttcaca cttgccgca gccgacctt tatcattgt 
      catgagtgt taccaattg tgttacaac cctattccg 
      tgtgaaata tgcagccaa acgttgtat tgtggtgaa 
      gaatgtcgg gacaaagcc cgggaaaag taccaccaa 
      tacgaatgc ccgattttg atttccttg aaaaacata 
      gtgggaaaa cacaaggct tttctctta gccatcaaa 
      atgtcgttt atgatttct gatgaaaat gacgttccg 
      gaggtttac gctttggtg gagaacctc agtcgggac 
      aacaacgat gaggttttc accacggcg ttaataaca 
      gctttgatg tatcatctg gtgaaaacc tacaccgga 
      aaatttccc gaagacgac ctggaagct gaaaataaa 
      ttcaaacac ttccttatg acccatttg cgcatttgt 
      ttgacccat gccgctgga atcgatgaa ctttaccca 
      aaccaagtc tccgaaggg caagaacca ggccaagaa 
      ttgctatct ttcaaatcg gaaactgtg ggttgtgcc 
      ctttatccc ttctacgct cttttcaga cacgcttgt 
      tgccctaat gtttttgcc catcatcat ggtactcaa 
      cgtgtcctc agagcagtt cggacgatt cacgaagga 
      caagagtgt tttgtgtcc tatgggccc tattacgtc 
      gagcatagt aaacaagag cgcaaaagt cgtcttttg 
      tctcaatat catttcacg tgcaagtgt agagcgtgt 
      gaggaggat tggcctcaa ttggatctg aatccttat 
      tatttaacg tcaaagcaa gatttagac tttgtggcg 
      aaaatcagg aatgcgaat attgaagcg gctgttgag 
      gaaattcca aggtttttg gagcgtctg aaagggttg 
      gaagtgttc gagcctctg tcggagtta actctccaa 
      cagaaactc ttgtcttat tgttttgat atgcttggg 
      aataaaaga agaatcttg taa
    mRNA sequence
    • >TC005322 RA
      tcatccgcc atcgcgttc gtcttcaac cgttatagt 
      ttttgttct gtgttatta tttttgcaa aatggcatt 
      aaatcaggc tctaagaaa ttttctgaa atctaaaaa 
      tcgacaaga gcctgcaag tttcatcca cctgtttga 
      gttgtatac aagtctcga acacgatcc tcatgaaga 
      gaaagccct tcccaagtt cctgaaaga attaaaccg 
      gaagaaaac tgaagaaga gaggcttga tttcatgta 
      caacgtgct ccagaagta caacttgtt cagaaataa 
      gtttacgcg agatagaaa acgtgatga acatgcgga 
      acattgtaa actcgaagg caacgttta tttttctga 
      gggcaacta tagctttgc tttggggtt ctacacaaa 
      ggctcttat gggtgctaa tggaccaga aatgctggc 
      tatatgtta cgctaatcg ttcagctgc tttgctcaa 
      gctggggtt gtatgaaga gtgtctgat tgacattaa 
      aagagctat agatcacaa ttatcccga acgtttgag 
      accgaaatt aatgtcgcg gcgtgtgaa agcagttgc 
      acttaaaag aaaacaaaa attcgaaag tacttatta 
      tgaggaggc tcctaaaat tcccacttc tcaacgaaa 
      caattcaat tccttgtgc aaccaattc ggttgagat 
      taaagtcga tgagaaatt gcgtaaacg tgttgttgc 
      cactaaaga catccaaat tggacaagt gattgcggt 
      agaaacgcc ttgtgttgc agcgttaat taacgttgt 
      gctgtttca ttgtcatga ctgttacat tctttgcta 
      caacccaat tccttgtaa aacttgcac agaagtggt 
      ctactgtag tgaagcatg cagggaaaa tgccttcgc 
      caagtacca ccagaaaga gtgccccat ttacttatc 
      tatgagaaa attagtggg aattgatac tcattttca 
      atgggcttt gaaaatgac tcttcttgt tcaaacaca 
      agcggataa gtgttgtga tagaataga tacagatga 
      cgatcgtac caggaaaac attaatggc aaccctgtt 
      ttaccatct tatcaagaa ctgtacaac agttttggc 
      tggagttga tgaagctgg tgtgaagaa ttttaaaag 
      ggttttgat gtcgtacat gcacatttg cgactatca 
      cgtttctga tattgatga gatttttgt tcatggggg 
      atctcgtga cttggaatt aaaacagga aacttttgc 
      aaaggccat gtatccgtt ttctgacaa attgaggca 
      ctcttgttg ccccaatgt tatgggctg gtatcatgg 
      agtgacaag agtattaag ggccattag gaccatcaa 
      aaaggggga agagtgttt tttctctta tgggcctct 
      ttatacgaa cattgagaa agaagagcg ccaaaatta 
      catcttttt tatttacaa ctttaaatg cgcatgccg 
      tgcttgtaa acaaaattg gcctcagac tgaaagtgt 
      gcctcgagt agcgatcga agaccacat gtttctata 
      cacaaggaa gagatttga tgtggatgc tgctaaaat 
      gcttattaa cagaaaaat tgacttgat gaagcaact 
      ggaaaggag tgaaccaaa tgatgaatt gattaacca 
      gcaagaaat gctgttcca ctgcttcta cattttggg 
      aaataagcg agcgtttca aagtcagct cctggcttg 
      tgtgcaaga tgacactcg caattcggg agagtttgt 
      gcgcgattt tcacaagtt gggaagtaa tgtagagaa 
      agtggattt tttgtatga gaggatgcg acagtacgg 
      cttgcttga cgacaaatt taccaagga tgaaaaaga 
      tgataagaa agcgaagaa acacaagga aaagggcga 
      taagttttt tgcaactgg ggctttttc caaagccct 
      tcagtctta tactaggag cgtcatgaa ggccaaaag 
      ctcggaagc cttggctat ggcttacgc caaccgatc 
      tgcagccct gttcaaact cgaacagtt cgaggaggc 
      tctaactga tgtagaaag agctctttc tggcgacta 
      ccctgaaaa tctgaggcc cagtttggt gacgcgtcg 
      cgagaaact tttgggttt tatgggtgc ccatggccc 
      tgcattcaa tttctttga atctgttcc tgaaattcc 
      agaggagca gaaaaatgc ctcaattcc gagcgctag 
      ctcctgtat tgaaataaa accagacga aaagtcacg 
      tcgccgtgt tttcgcagc tcggaaaat cgaaatcgg 
      cgaaataat agcggtaga aaaaccgtt tgttttcac 
      acttgccgc agccgacct ttatcattg tcatgagtg 
      ttaccaatt gtgttacaa ccctattcc gtgtgaaat 
      atgcagcca aacgttgta ttgtggtga agaatgtcg 
      ggacaaagc ccgggaaaa gtaccacca atacgaatg 
      cccgatttt gatttcctt gaaaaacat agtgggaaa 
      acacaaggc ttttctctt agccatcaa aatgtcgtt 
      tatgatttc tgatgaaaa tgacgttcc ggaggttta 
      cgctttggt ggagaacct cagtcggga caacaacga 
      tgaggtttt caccacggc gttaataac agctttgat 
      gtatcatct ggtgaaaac ctacaccgg aaaatttcc 
      cgaagacga cctggaagc tgaaaataa attcaaaca 
      cttccttat gacccattt gcgcatttg tttgaccca 
      tgccgctgg aatcgatga actttaccc aaaccaagt 
      ctccgaagg gcaagaacc aggccaaga attgctatc 
      tttcaaatc ggaaactgt gggttgtgc cctttatcc 
      cttctacgc tcttttcag acacgcttg ttgccctaa 
      tgtttttgc ccatcatca tggtactca acgtgtcct 
      cagagcagt tcggacgat tcacgaagg acaagagtg 
      ttttgtgtc ctatgggcc ctattacgt cgagcatag 
      taaacaaga gcgcaaaag tcgtctttt gtctcaata 
      tcatttcac gtgcaagtg tagagcgtg tgaggagga 
      ttggcctca attggatct gaatcctta ttatttaac 
      gtcaaagca agatttaga ctttgtggc gaaaatcag 
      gaatgcgaa tattgaagc ggctgttga ggaaattcc 
      aaggttttt ggagcgtct gaaagggtt ggaagtgtt 
      cgagcctct gtcggagtt aactctcca acagaaact 
      cttgtctta ttgttttga tatgcttgg gaataaaag 
      aagaatctt gtaattttt tttcattaa taaatattt 
      gaagtaatg