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Gene information
General information
TC005319 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14500751..14504743
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005319
    iBeetle screen iB_00827

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 5
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 87.5% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    wings not closed - Number of animals showing the phenotype: 7

    oblique dorsal thoracic muscles thinner - Number of animals showing the phenotype: 7

    Adult morphology
    wings not closed - Number of animals showing the phenotype: 2

    mesothoracic leg cuticle partially colour lighter - Number of animals showing the phenotype: 2


    elytra colour lighter - Number of animals showing the phenotype: 2

    motility decreased - (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005319 RA
      MWRNSLRFL VKLPQQSKK LAVQSYNSL QWAPFRDSG 
      LQRFISTTP KLSMFIQTQ ETPNPNSLK FLPGVKVLE 
      EGQTIDFPN GQAAYCSPL GKLLFRIEG VKSVFLGPE 
      FITVTKTDD EIEWKIIKP EIFATIMDF FASGLPVLN 
      DATPNADTQ INEDDSEIV QMIKELLDT RIRPTVQED 
      GGDIIFMGY DDGIVKLKL QGACTSCPS SIVTLKNGV 
      QNMLQFYIP EVLGVEQVQ DEELCKIEA K
    CDS sequence
    • >TC005319 RA
      atgtggcgt aattcactt cgatttttg gtaaagttg 
      cctcagcaa tccaagaaa cttgctgtg cagtcctac 
      aattccctt cagtgggcc cctttccga gacagtgga 
      ctccaacgc tttatctca accactcca aagttatca 
      atgtttatt cagactcaa gagacccca aatccaaac 
      agtctcaag tttttgcca ggggttaaa gttctagag 
      gagggccaa actatagat tttcctaac ggacaagcg 
      gcttattgt tcacctttg ggaaaatta cttttccga 
      attgaaggt gttaaaagt gtttttctg ggaccggaa 
      tttatcact gttactaaa accgatgat gaaattgaa 
      tggaaaatt attaaacct gagattttt gccacaatt 
      atggacttt tttgccagc ggattaccg gtcttgaat 
      gacgccacc ccgaacgca gacacacaa atcaacgaa 
      gatgatagt gaaattgtt caaatgatt aaggaacta 
      ttagacaca aggattagg ccaactgta caagaggat 
      ggaggtgac attattttc atggggtat gacgacggt 
      atagttaaa ttaaaactg caaggggct tgcactagt 
      tgcccgagt tcaatagtt acactcaaa aatggtgtc 
      caaaatatg ctgcagttt tatattcct gaagttttg 
      ggagtggaa caagtgcaa gatgaggaa ctgtgcaag 
      atcgaagcg aagtaa
    mRNA sequence
    • >TC005319 RA
      ataaaattc aacatgtgc aaacacata cacctattg 
      tgtagatat ctcaaacaa cagaacact tccctaact 
      gctagtact ggtctgtca ggtaatatc cttctctaa 
      atctcattt ttacttttt ggctgccta aatttaatc 
      agaagaaca aaaaagata aattcttat aggttcaga 
      tgatgttac atcgatatt atagtcatt tgatcatct 
      gtgatactc caccaacgt ttttgggat cattgtaaa 
      aaaaacaga aggtaaatt ttcttggga aggttaggg 
      gctggggcc tagcggtga gggtgcaaa ataatgtca 
      ttatgaatt attagtagt aaactgggt gtagggggg 
      attttgtgg tgggactaa catgttaat acctacctg 
      ttttcaaca cagatgaac gtaatttgc tgatcagcg 
      aagattcca aagcctctg aagtttccg gaattgtaa 
      ccggcctta ccgtcaatg aagctctaa accccaaag 
      agtttgaca agcagttta ttgccaaag aaaggcaga 
      gactgcgtg aaactttta atcaactgg tagaaggta 
      tttgtacgc atcgttaac ccgaaatcg atttattgt 
      ttgatttac tttaatatt tgtgggcat ctgagacta 
      ttttttcat aaccaacct agcaaatag tttttttca 
      ctgatcgaa ctatatatg actcaaaca taaataaat 
      aaaaaagaa atcagtccc gtacgaata ataaaggtc 
      aatattttg aaaattcgt aaacagaaa cgtcaggaa 
      ctaataaaa acacaagta gcaattata aataacgga 
      ttttattag actgattta taaatgtac ccctgtggc 
      aatatcaga ataatttat cggataact atttcagtt 
      ttaagaaaa cagagtcac ttttcatga taaacaaaa 
      gcgataaat aaaagttta atgccatac atttatttg 
      cattaatta tgaaaattt gaaaaccgc gctttttat 
      ataaacaga atttaaaaa gccaaactc ttgcagtgt 
      aattttccg tagccttaa attttccca cctttgtga 
      atttaatta ccaaagaca tattgtaaa ttctccaat 
      ttaagcaga aaatattga ctcaaccaa tctgtcaaa 
      tgtcggtat tctaaccat aaaattaaa ataagcaaa 
      tcttaaaat gtggcgtaa ttcacttcg atttttggt 
      aaagttgcc tcagcaatc caagaaact tgctgtgca 
      gtcctacaa ttcccttca gtgggcccc tttccgaga 
      cagtggact ccaacgctt tatctcaac cactccaaa 
      gttatcaat gtttattca gactcaaga gaccccaaa 
      tccaaacag tctcaagtt tttgccagg ggttaaagt 
      tctagagga gggccaaac tatagattt tcctaacgg 
      acaagcggc ttattgttc acctttggg aaaattact 
      tttccgaat tgaaggtgt taaaagtgt ttttctggg 
      accggaatt tatcactgt tactaaaac cgatgatga 
      aattgaatg gaaaattat taaacctga gatttttgc 
      cacaattat ggacttttt tgccagcgg attaccggt 
      cttgaatga cgccacccc gaacgcaga cacacaaat 
      caacgaaga tgatagtga aattgttca aatgattaa 
      ggaactatt agacacaag gattaggcc aactgtaca 
      agaggatgg aggtgacat tattttcat ggggtatga 
      cgacggtat agttaaatt aaaactgca aggggcttg 
      cactagttg cccgagttc aatagttac actcaaaaa 
      tggtgtcca aaatatgct gcagtttta tattcctga 
      agttttggg agtggaaca agtgcaaga tgaggaact 
      gtgcaagat cgaagcgaa gtaatttgc aaactaatc 
      attgattat tgtacttaa gtgattaat tatattaat 
      attaatcaa ataaacgtg ttaatccta t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY