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Gene information
General information
TC005316 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid58: 1321863..1327678
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005316
    iBeetle screen iB_00826
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg present - Number of animals showing the phenotype: 4

    tropharium irregular - Number of animals showing the phenotype: 4
    egg chamber mostly not present - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Pupal morphology
    antenna position irregular - Number of animals showing the phenotype: 5

    wings shortened - Number of animals showing the phenotype: 5
    wings not closed - Number of animals showing the phenotype: 5

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005316 RA
      MNHVTGQYR RNDDTSAEV EEMETQEVT TTDSTDTGG 
      GEMDQQNGE VIIGPQPDP GMTVQDADM EEDESRSEA 
      TFRYTVQQF SKLKDSALS PACYVRNLP WKIMVMPRN 
      SHGQDRTAQ RSLGFFLQC NGESESSSW SCYAVAELR 
      LLSVRPDVE PFSRKIQHL FYSKENDWG FSHFMAWNE 
      VLDPEKGYI KDDAITLEV HVVADAPHG VSWDSKKHT 
      GYVGLKNQG ATCYMNSLL QTLYFTNQL RKAVYKMPT 
      ESDDSTKSV ALALQRVFH ELQFCDKPV GTKKLTKSF 
      GWETLDSFM QHDVQEFLR VLLDKLESK MKGTCVEGT 
      VPKLFEGKM ISYIRCKNV DYSSTRSET FYDIQLNIK 
      GKKNIDESF KDYIAKETL DGDNKYDAG EHGLQDAEK 
      GVIFSAFPP VLHLHLMRF QYDPVTDCS VKFNDRFEF 
      YEKISLDAY LQEPDPNNP ANYTLHAVL VHSGDNHGG 
      HYVVFINPR GDGKWCKFD DDVVSKCTK QEAIEHNYG 
      GHDEDMNMT VKHCTNAYM LVYIRDSEL HNVLQEVTD 
      ADIPSELAD RLAEEKRME QVRRKERNE AHLYMTINV 
      LLEDSFDGH QGNDLYDPD RPLFRVFKI KKMALVSEM 
      MDMFADAFK YPTEQIRPW PFSQRSNQT MRPCMLDLD 
      TESHKAIID SAENQNPWT IFLELLPPD SGLTALPNF 
      DKETDVLLF FKMYDPKQK KIHYCGHSY LPVTSKLGD 
      LIPMLNERA GFPPDTELV LYEEIRPNM IEKITNYSD 
      PLEKVLDEL MDGDIILFE KEEREEFSD LPTCIDYFK 
      DLYYRVEVT FVDKCTPND PGFTMELSQ RMTYDQLAR 
      AVAQRVGTD PYLLQFFKC QNYKDSPGH PLRCTFEGT 
      LKDLLVFSK PKAPKKIFY QQLSIRVNE LENKKQFKC 
      LYVGPNVFE EKELILYPN KRGTVSDLL EEAKKQIEF 
      GEGSTGKLR FTEVSCNKV ALGPKEDTP LDHLVINAA 
      KVYRIEQVP RDELHINED EMLISCAHF QKEVFSTFG 
      SPFLFKIKQ GEPFTKVKE RIQKRLGVP DKEFEKYKF 
      SIVAMGRQQ VLQDDEYVV NLADFRPLP NQAGSPKPW 
      LGLDHMNKA PKRSRFNYL EKAIKIYN
    CDS sequence
    • >TC005316 RA
      atgaaccac gttacgggc cagtacaga cgcaacgac 
      gacacttcc gccgaagtg gaggagatg gaaacacag 
      gaagtcacc accaccgat tctacggac actgggggc 
      ggtgaaatg gaccaacag aacggcgaa gtcatcatc 
      gggccacag cccgaccct ggaatgaca gttcaagac 
      gcagacatg gaagaggac gaatcgcgt tcagaagcc 
      acctttcgc tacactgtc caacagttt agcaaactg 
      aaagactct gctctgtct ccggcatgt tacgtacgt 
      aacctccct tggaaaatc atggttatg ccccggaac 
      agtcatgga caggatcgg actgcacag cgctctttg 
      ggctttttc cttcagtgt aacggcgaa agcgagtcc 
      tcttcgtgg tcttgctat gccgttgct gagctgaga 
      ctgctctca gttcgacca gatgttgag cctttctcg 
      cgaaaaatt caacatttg ttctattcg aaggaaaat 
      gattgggga ttttcacat tttatggcg tggaatgag 
      gtcttagac ccggagaaa ggctatatt aaagatgac 
      gcgattact ttggaagta catgtggtg gctgatgcc 
      ccacatgga gtctcatgg gacagtaaa aaacataca 
      ggttacgtg ggtttaaaa aatcaagga gcgacctgt 
      tacatgaac tcacttctt caaacttta tattttaca 
      aatcagtta cgaaaagcc gtttataaa atgccaacc 
      gaatcagat gattcgact aaatctgta gcacttgct 
      ctacagcga gtctttcac gaactacaa ttctgcgat 
      aaaccagta ggtacgaag aaattgacg aaaagtttc 
      ggttgggaa acgttagat tcatttatg caacatgat 
      gtgcaggaa tttctcagg gtactttta gataaactt 
      gaaagtaag atgaaggga acttgtgtg gagggcacc 
      gttccgaaa cttttcgaa ggtaaaatg atttcgtac 
      attcggtgt aagaacgtg gattattcg agcacacgt 
      tcggagacg ttctatgat attcagttg aatattaag 
      gggaagaaa aatatcgat gagtcgttt aaggattac 
      atcgcaaag gagactcta gatggcgat aataaatac 
      gacgcaggt gaacatggg ttgcaagat gctgaaaaa 
      ggggtgatt ttttcggct tttcctcct gttttgcac 
      ttgcatttg atgcggttt caatacgat cccgtcact 
      gactgttcc gttaaattc aatgatcgt tttgaattt 
      tacgaaaag atttcgtta gatgcttat ttgcaagaa 
      ccggatcct aacaatccg gccaactat actctacat 
      gcagttcta gtacattca ggcgacaat cacggtggt 
      cattatgtt gtttttata aatcctaga ggtgatggc 
      aagtggtgt aaatttgac gatgatgtt gtctccaaa 
      tgtacgaaa caagaagca atcgaacat aactacggc 
      ggacatgat gaggatatg aacatgaca gtgaaacat 
      tgtacaaat gcgtacatg ttggtatac atcagagat 
      tcagagttg cataacgta ttacaagaa gtaaccgat 
      gctgatata ccaagtgaa ttagctgat cgtttggct 
      gaagaaaaa cgaatggaa caagtgaga cgaaaagaa 
      cgcaacgaa gcccatctc tatatgact atcaatgtt 
      ttgcttgaa gatagtttt gatggacat cagggtaat 
      gatttatac gatcctgat cgtccacta tttcgtgtt 
      tttaaaatc aaaaagatg gcactagtg agcgaaatg 
      atggatatg ttcgccgat gcgtttaaa tatccaact 
      gaacaaata agaccatgg ccatttagt caacgatca 
      aatcagaca atgaggcct tgtatgtta gatctagac 
      actgagtcg cataaagct attatagat tcagctgaa 
      aaccagaat ccttggact atatttctc gagttgttg 
      ccacctgat tcgggctta actgcgtta ccaaatttt 
      gacaaagag accgatgtt ttgttgttt ttcaaaatg 
      tatgatccg aaacagaag aaaatacat tattgtgga 
      catagttac ttaccggtt actagtaaa ttaggagat 
      ttgataccg atgttgaat gagagggca gggtttccg 
      ccggatacg gagttggtc ttgtatgag gagattagg 
      cctaatatg attgagaaa attactaat tatagtgat 
      ccgttggaa aaggttttg gacgagttg atggatggc 
      gatataatc ctgttcgag aaagaagaa cgtgaggag 
      ttttctgac ttgccaacc tgtatcgac tacttcaaa 
      gacctatat tatcgtgtc gaggtaaca tttgtcgac 
      aaatgtaca ccgaacgat cccggtttt acaatggaa 
      ttatctcaa agaatgact tatgatcag ttggcacgt 
      gctgtagct cagagggtc ggtaccgat ccttactta 
      ttacaattt tttaaatgt caaaattac aaagatagt 
      cctggacat cctttaagg tgcactttt gagggcacc 
      ttgaaagat ctcttagtt ttttccaag ccaaaggcg 
      cctaagaaa atcttttat caacagttg agtattcgt 
      gttaacgaa ttagaaaat aaaaaacaa tttaaatgt 
      ctttatgtg ggaccgaat gtgtttgaa gagaaggag 
      ttaattttg tatccgaat aagagaggg acggtaagt 
      gatttgttg gaagaggcg aagaaacaa atcgagttt 
      ggtgaagga agtacaggg aaattaagg tttaccgaa 
      gttagttgt aataaagta gcactgggt cccaaagaa 
      gatacgccg ctagatcat ttggtaata aatgcagca 
      aaggtttat agaattgaa caagtgcca agagatgag 
      ttgcacata aacgaggac gaaatgctt atttcttgt 
      gcgcatttt caaaaggaa gttttttcg acttttggc 
      tcccctttc ttatttaaa attaaacaa ggggagcct 
      tttaccaag gtcaaagaa cgtattcaa aagcgactt 
      ggtgttcct gataaggaa ttcgaaaag tacaagttt 
      tctatcgtt gctatgggc cgacaacag gtccttcaa 
      gacgacgaa tatgtagtt aatttagcc gattttaga 
      ccacttccg aatcaagct gggagtccc aaaccatgg 
      ttgggcctg gatcatatg aacaaggcc ccgaaaaga 
      tcacgtttt aattattta gaaaaagcc atcaaaatt 
      tacaattga
    mRNA sequence
    • >TC005316 RA
      ttgccagta tttttttcc ttaaaatta attaaagaa 
      aaactgtgt tcggtttcg aattgccaa tatgacagt 
      tgtgggtgc atttgttta aaattgttg taaattgtg 
      taacttgtg gaaagtgcg ttattttgt gccatttcc 
      ggtgttttt tgtgaatta gcgcgggaa aattcgtat 
      ctgggcccc ccatttctt gcgccctcg tcgcatacg 
      catccgcgc agtcgattg cagaaaccg gtgttcgtc 
      gtcgagtgt tgtgcccac catcgctat ttcaatgca 
      aagaaaatg cctccattt tgtttattt ttctcattg 
      aaaagctca attagcgtt tccaaataa aatcgagtg 
      agtgtttca agtgtgaaa agtccgtca cgtgtcaca 
      actctctat atatgtgtg ttgtgtgca gccctctct 
      cagagggta caaaaacca gcaagaatt ggtcgcgtc 
      acgagtaag cactttgtc aaattgact acggtctct 
      ggcactaaa aataatacc cagaataac cacggtggt 
      aactgttgc gttgcacag tcggacaaa gaccttttg 
      aatagcaac aaaaagaac aaaggtgtg ttcgggttc 
      gcgaaagac agaggggct gtcaagacg ccagcgctc 
      aatatgaac cacgttacg ggccagtac agacgcaac 
      gacgacact tccgccgaa gtggaggag atggaaaca 
      caggaagtc accaccacc gattctacg gacactggg 
      ggcggtgaa atggaccaa cagaacggc gaagtcatc 
      atcgggcca cagcccgac cctggaatg acagttcaa 
      gacgcagac atggaagag gacgaatcg cgttcagaa 
      gccaccttt cgctacact gtccaacag tttagcaaa 
      ctgaaagac tctgctctg tctccggca tgttacgta 
      cgtaacctc ccttggaaa atcatggtt atgccccgg 
      aacagtcat ggacaggat cggactgca cagcgctct 
      ttgggcttt ttccttcag tgtaacggc gaaagcgag 
      tcctcttcg tggtcttgc tatgccgtt gctgagctg 
      agactgctc tcagttcga ccagatgtt gagcctttc 
      tcgcgaaaa attcaacat ttgttctat tcgaaggaa 
      aatgattgg ggattttca cattttatg gcgtggaat 
      gaggtctta gacccggag aaaggctat attaaagat 
      gacgcgatt actttggaa gtacatgtg gtggctgat 
      gccccacat ggagtctca tgggacagt aaaaaacat 
      acaggttac gtgggttta aaaaatcaa ggagcgacc 
      tgttacatg aactcactt cttcaaact ttatatttt 
      acaaatcag ttacgaaaa gccgtttat aaaatgcca 
      accgaatca gatgattcg actaaatct gtagcactt 
      gctctacag cgagtcttt cacgaacta caattctgc 
      gataaacca gtaggtacg aagaaattg acgaaaagt 
      ttcggttgg gaaacgtta gattcattt atgcaacat 
      gatgtgcag gaatttctc agggtactt ttagataaa 
      cttgaaagt aagatgaag ggaacttgt gtggagggc 
      accgttccg aaacttttc gaaggtaaa atgatttcg 
      tacattcgg tgtaagaac gtggattat tcgagcaca 
      cgttcggag acgttctat gatattcag ttgaatatt 
      aaggggaag aaaaatatc gatgagtcg tttaaggat 
      tacatcgca aaggagact ctagatggc gataataaa 
      tacgacgca ggtgaacat gggttgcaa gatgctgaa 
      aaaggggtg attttttcg gcttttcct cctgttttg 
      cacttgcat ttgatgcgg tttcaatac gatcccgtc 
      actgactgt tccgttaaa ttcaatgat cgttttgaa 
      ttttacgaa aagatttcg ttagatgct tatttgcaa 
      gaaccggat cctaacaat ccggccaac tatactcta 
      catgcagtt ctagtacat tcaggcgac aatcacggt 
      ggtcattat gttgttttt ataaatcct agaggtgat 
      ggcaagtgg tgtaaattt gacgatgat gttgtctcc 
      aaatgtacg aaacaagaa gcaatcgaa cataactac 
      ggcggacat gatgaggat atgaacatg acagtgaaa 
      cattgtaca aatgcgtac atgttggta tacatcaga 
      gattcagag ttgcataac gtattacaa gaagtaacc 
      gatgctgat ataccaagt gaattagct gatcgtttg 
      gctgaagaa aaacgaatg gaacaagtg agacgaaaa 
      gaacgcaac gaagcccat ctctatatg actatcaat 
      gttttgctt gaagatagt tttgatgga catcagggt 
      aatgattta tacgatcct gatcgtcca ctatttcgt 
      gtttttaaa atcaaaaag atggcacta gtgagcgaa 
      atgatggat atgttcgcc gatgcgttt aaatatcca 
      actgaacaa ataagacca tggccattt agtcaacga 
      tcaaatcag acaatgagg ccttgtatg ttagatcta 
      gacactgag tcgcataaa gctattata gattcagct 
      gaaaaccag aatccttgg actatattt ctcgagttg 
      ttgccacct gattcgggc ttaactgcg ttaccaaat 
      tttgacaaa gagaccgat gttttgttg tttttcaaa 
      atgtatgat ccgaaacag aagaaaata cattattgt 
      ggacatagt tacttaccg gttactagt aaattagga 
      gatttgata ccgatgttg aatgagagg gcagggttt 
      ccgccggat acggagttg gtcttgtat gaggagatt 
      aggcctaat atgattgag aaaattact aattatagt 
      gatccgttg gaaaaggtt ttggacgag ttgatggat 
      ggcgatata atcctgttc gagaaagaa gaacgtgag 
      gagttttct gacttgcca acctgtatc gactacttc 
      aaagaccta tattatcgt gtcgaggta acatttgtc 
      gacaaatgt acaccgaac gatcccggt tttacaatg 
      gaattatct caaagaatg acttatgat cagttggca 
      cgtgctgta gctcagagg gtcggtacc gatccttac 
      ttattacaa ttttttaaa tgtcaaaat tacaaagat 
      agtcctgga catccttta aggtgcact tttgagggc 
      accttgaaa gatctctta gttttttcc aagccaaag 
      gcgcctaag aaaatcttt tatcaacag ttgagtatt 
      cgtgttaac gaattagaa aataaaaaa caatttaaa 
      tgtctttat gtgggaccg aatgtgttt gaagagaag 
      gagttaatt ttgtatccg aataagaga gggacggta 
      agtgatttg ttggaagag gcgaagaaa caaatcgag 
      tttggtgaa ggaagtaca gggaaatta aggtttacc 
      gaagttagt tgtaataaa gtagcactg ggtcccaaa 
      gaagatacg ccgctagat catttggta ataaatgca 
      gcaaaggtt tatagaatt gaacaagtg ccaagagat 
      gagttgcac ataaacgag gacgaaatg cttatttct 
      tgtgcgcat tttcaaaag gaagttttt tcgactttt 
      ggctcccct ttcttattt aaaattaaa caaggggag 
      ccttttacc aaggtcaaa gaacgtatt caaaagcga 
      cttggtgtt cctgataag gaattcgaa aagtacaag 
      ttttctatc gttgctatg ggccgacaa caggtcctt 
      caagacgac gaatatgta gttaattta gccgatttt 
      agaccactt ccgaatcaa gctgggagt cccaaacca 
      tggttgggc ctggatcat atgaacaag gccccgaaa 
      agatcacgt tttaattat ttagaaaaa gccatcaaa 
      atttacaat tgagagact gagtgagga agcttattt 
      tcacaacct tgcactttt cggctctgc agtattgcc 
      aattgatcg tgatttccc taaagaatc gataaatgt 
      aaggtgcgg ctgctttgt catttggtg ctttgtgtg 
      agcacaagt cttctgctg tgtgacacc attttgtat 
      ataaaatat atatttgtt ctgtctgtc tatttagaa 
      tatattcgc gctaaatgc tgaatgcta gctgtttat 
      ttacttcgg tttcgagtt attttcttt gaattaatt 
      gaattaact gtttgtaga tttttccgg taatgagac 
      ggtttgaat catgttgaa aataattat tatttgaac 
      taaatcatt aagggagct gcgattttt ttacattaa 
      atttataaa ttgtgatgt tggttgtgt aacattttg 
      tttttaata agcatgaaa tatatgcaa ctgattttt 
      cactgcgtt ctactatgt ttctcaagc tgtaaaaat 
      tatatactt tattgttat ttgaaactt tatgtaaac 
      acattttat aatagaata aagaactaa agtatta