General information | |||
TC005316 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 14508658..14514473 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC005316 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00826 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
tropharium irregular - Number of animals showing the phenotype: 4
egg chamber mostly not present - Number of animals showing the phenotype: 4
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (19 dpi)
- >TC005316 RA
MNHVTGQYR RNDDTSAEV EEMETQEVT TTDSTDTGG GEMDQQNGE VIIGPQPDP GMTVQDADM EEDESRSEA TFRYTVQQF SKLKDSALS PACYVRNLP WKIMVMPRN SHGQDRTAQ RSLGFFLQC NGESESSSW SCYAVAELR LLSVRPDVE PFSRKIQHL FYSKENDWG FSHFMAWNE VLDPEKGYI KDDAITLEV HVVADAPHG VSWDSKKHT GYVGLKNQG ATCYMNSLL QTLYFTNQL RKAVYKMPT ESDDSTKSV ALALQRVFH ELQFCDKPV GTKKLTKSF GWETLDSFM QHDVQEFLR VLLDKLESK MKGTCVEGT VPKLFEGKM ISYIRCKNV DYSSTRSET FYDIQLNIK GKKNIDESF KDYIAKETL DGDNKYDAG EHGLQDAEK GVIFSAFPP VLHLHLMRF QYDPVTDCS VKFNDRFEF YEKISLDAY LQEPDPNNP ANYTLHAVL VHSGDNHGG HYVVFINPR GDGKWCKFD DDVVSKCTK QEAIEHNYG GHDEDMNMT VKHCTNAYM LVYIRDSEL HNVLQEVTD ADIPSELAD RLAEEKRME QVRRKERNE AHLYMTINV LLEDSFDGH QGNDLYDPD RPLFRVFKI KKMALVSEM MDMFADAFK YPTEQIRPW PFSQRSNQT MRPCMLDLD TESHKAIID SAENQNPWT IFLELLPPD SGLTALPNF DKETDVLLF FKMYDPKQK KIHYCGHSY LPVTSKLGD LIPMLNERA GFPPDTELV LYEEIRPNM IEKITNYSD PLEKVLDEL MDGDIILFE KEEREEFSD LPTCIDYFK DLYYRVEVT FVDKCTPND PGFTMELSQ RMTYDQLAR AVAQRVGTD PYLLQFFKC QNYKDSPGH PLRCTFEGT LKDLLVFSK PKAPKKIFY QQLSIRVNE LENKKQFKC LYVGPNVFE EKELILYPN KRGTVSDLL EEAKKQIEF GEGSTGKLR FTEVSCNKV ALGPKEDTP LDHLVINAA KVYRIEQVP RDELHINED EMLISCAHF QKEVFSTFG SPFLFKIKQ GEPFTKVKE RIQKRLGVP DKEFEKYKF SIVAMGRQQ VLQDDEYVV NLADFRPLP NQAGSPKPW LGLDHMNKA PKRSRFNYL EKAIKIYN
- >TC005316 RA
atgaaccac gttacgggc cagtacaga cgcaacgac gacacttcc gccgaagtg gaggagatg gaaacacag gaagtcacc accaccgat tctacggac actgggggc ggtgaaatg gaccaacag aacggcgaa gtcatcatc gggccacag cccgaccct ggaatgaca gttcaagac gcagacatg gaagaggac gaatcgcgt tcagaagcc acctttcgc tacactgtc caacagttt agcaaactg aaagactct gctctgtct ccggcatgt tacgtacgt aacctccct tggaaaatc atggttatg ccccggaac agtcatgga caggatcgg actgcacag cgctctttg ggctttttc cttcagtgt aacggcgaa agcgagtcc tcttcgtgg tcttgctat gccgttgct gagctgaga ctgctctca gttcgacca gatgttgag cctttctcg cgaaaaatt caacatttg ttctattcg aaggaaaat gattgggga ttttcacat tttatggcg tggaatgag gtcttagac ccggagaaa ggctatatt aaagatgac gcgattact ttggaagta catgtggtg gctgatgcc ccacatgga gtctcatgg gacagtaaa aaacataca ggttacgtg ggtttaaaa aatcaagga gcgacctgt tacatgaac tcacttctt caaacttta tattttaca aatcagtta cgaaaagcc gtttataaa atgccaacc gaatcagat gattcgact aaatctgta gcacttgct ctacagcga gtctttcac gaactacaa ttctgcgat aaaccagta ggtacgaag aaattgacg aaaagtttc ggttgggaa acgttagat tcatttatg caacatgat gtgcaggaa tttctcagg gtactttta gataaactt gaaagtaag atgaaggga acttgtgtg gagggcacc gttccgaaa cttttcgaa ggtaaaatg atttcgtac attcggtgt aagaacgtg gattattcg agcacacgt tcggagacg ttctatgat attcagttg aatattaag gggaagaaa aatatcgat gagtcgttt aaggattac atcgcaaag gagactcta gatggcgat aataaatac gacgcaggt gaacatggg ttgcaagat gctgaaaaa ggggtgatt ttttcggct tttcctcct gttttgcac ttgcatttg atgcggttt caatacgat cccgtcact gactgttcc gttaaattc aatgatcgt tttgaattt tacgaaaag atttcgtta gatgcttat ttgcaagaa ccggatcct aacaatccg gccaactat actctacat gcagttcta gtacattca ggcgacaat cacggtggt cattatgtt gtttttata aatcctaga ggtgatggc aagtggtgt aaatttgac gatgatgtt gtctccaaa tgtacgaaa caagaagca atcgaacat aactacggc ggacatgat gaggatatg aacatgaca gtgaaacat tgtacaaat gcgtacatg ttggtatac atcagagat tcagagttg cataacgta ttacaagaa gtaaccgat gctgatata ccaagtgaa ttagctgat cgtttggct gaagaaaaa cgaatggaa caagtgaga cgaaaagaa cgcaacgaa gcccatctc tatatgact atcaatgtt ttgcttgaa gatagtttt gatggacat cagggtaat gatttatac gatcctgat cgtccacta tttcgtgtt tttaaaatc aaaaagatg gcactagtg agcgaaatg atggatatg ttcgccgat gcgtttaaa tatccaact gaacaaata agaccatgg ccatttagt caacgatca aatcagaca atgaggcct tgtatgtta gatctagac actgagtcg cataaagct attatagat tcagctgaa aaccagaat ccttggact atatttctc gagttgttg ccacctgat tcgggctta actgcgtta ccaaatttt gacaaagag accgatgtt ttgttgttt ttcaaaatg tatgatccg aaacagaag aaaatacat tattgtgga catagttac ttaccggtt actagtaaa ttaggagat ttgataccg atgttgaat gagagggca gggtttccg ccggatacg gagttggtc ttgtatgag gagattagg cctaatatg attgagaaa attactaat tatagtgat ccgttggaa aaggttttg gacgagttg atggatggc gatataatc ctgttcgag aaagaagaa cgtgaggag ttttctgac ttgccaacc tgtatcgac tacttcaaa gacctatat tatcgtgtc gaggtaaca tttgtcgac aaatgtaca ccgaacgat cccggtttt acaatggaa ttatctcaa agaatgact tatgatcag ttggcacgt gctgtagct cagagggtc ggtaccgat ccttactta ttacaattt tttaaatgt caaaattac aaagatagt cctggacat cctttaagg tgcactttt gagggcacc ttgaaagat ctcttagtt ttttccaag ccaaaggcg cctaagaaa atcttttat caacagttg agtattcgt gttaacgaa ttagaaaat aaaaaacaa tttaaatgt ctttatgtg ggaccgaat gtgtttgaa gagaaggag ttaattttg tatccgaat aagagaggg acggtaagt gatttgttg gaagaggcg aagaaacaa atcgagttt ggtgaagga agtacaggg aaattaagg tttaccgaa gttagttgt aataaagta gcactgggt cccaaagaa gatacgccg ctagatcat ttggtaata aatgcagca aaggtttat agaattgaa caagtgcca agagatgag ttgcacata aacgaggac gaaatgctt atttcttgt gcgcatttt caaaaggaa gttttttcg acttttggc tcccctttc ttatttaaa attaaacaa ggggagcct tttaccaag gtcaaagaa cgtattcaa aagcgactt ggtgttcct gataaggaa ttcgaaaag tacaagttt tctatcgtt gctatgggc cgacaacag gtccttcaa gacgacgaa tatgtagtt aatttagcc gattttaga ccacttccg aatcaagct gggagtccc aaaccatgg ttgggcctg gatcatatg aacaaggcc ccgaaaaga tcacgtttt aattattta gaaaaagcc atcaaaatt tacaattga
- >TC005316 RA
ttgccagta tttttttcc ttaaaatta attaaagaa aaactgtgt tcggtttcg aattgccaa tatgacagt tgtgggtgc atttgttta aaattgttg taaattgtg taacttgtg gaaagtgcg ttattttgt gccatttcc ggtgttttt tgtgaatta gcgcgggaa aattcgtat ctgggcccc ccatttctt gcgccctcg tcgcatacg catccgcgc agtcgattg cagaaaccg gtgttcgtc gtcgagtgt tgtgcccac catcgctat ttcaatgca aagaaaatg cctccattt tgtttattt ttctcattg aaaagctca attagcgtt tccaaataa aatcgagtg agtgtttca agtgtgaaa agtccgtca cgtgtcaca actctctat atatgtgtg ttgtgtgca gccctctct cagagggta caaaaacca gcaagaatt ggtcgcgtc acgagtaag cactttgtc aaattgact acggtctct ggcactaaa aataatacc cagaataac cacggtggt aactgttgc gttgcacag tcggacaaa gaccttttg aatagcaac aaaaagaac aaaggtgtg ttcgggttc gcgaaagac agaggggct gtcaagacg ccagcgctc aatatgaac cacgttacg ggccagtac agacgcaac gacgacact tccgccgaa gtggaggag atggaaaca caggaagtc accaccacc gattctacg gacactggg ggcggtgaa atggaccaa cagaacggc gaagtcatc atcgggcca cagcccgac cctggaatg acagttcaa gacgcagac atggaagag gacgaatcg cgttcagaa gccaccttt cgctacact gtccaacag tttagcaaa ctgaaagac tctgctctg tctccggca tgttacgta cgtaacctc ccttggaaa atcatggtt atgccccgg aacagtcat ggacaggat cggactgca cagcgctct ttgggcttt ttccttcag tgtaacggc gaaagcgag tcctcttcg tggtcttgc tatgccgtt gctgagctg agactgctc tcagttcga ccagatgtt gagcctttc tcgcgaaaa attcaacat ttgttctat tcgaaggaa aatgattgg ggattttca cattttatg gcgtggaat gaggtctta gacccggag aaaggctat attaaagat gacgcgatt actttggaa gtacatgtg gtggctgat gccccacat ggagtctca tgggacagt aaaaaacat acaggttac gtgggttta aaaaatcaa ggagcgacc tgttacatg aactcactt cttcaaact ttatatttt acaaatcag ttacgaaaa gccgtttat aaaatgcca accgaatca gatgattcg actaaatct gtagcactt gctctacag cgagtcttt cacgaacta caattctgc gataaacca gtaggtacg aagaaattg acgaaaagt ttcggttgg gaaacgtta gattcattt atgcaacat gatgtgcag gaatttctc agggtactt ttagataaa cttgaaagt aagatgaag ggaacttgt gtggagggc accgttccg aaacttttc gaaggtaaa atgatttcg tacattcgg tgtaagaac gtggattat tcgagcaca cgttcggag acgttctat gatattcag ttgaatatt aaggggaag aaaaatatc gatgagtcg tttaaggat tacatcgca aaggagact ctagatggc gataataaa tacgacgca ggtgaacat gggttgcaa gatgctgaa aaaggggtg attttttcg gcttttcct cctgttttg cacttgcat ttgatgcgg tttcaatac gatcccgtc actgactgt tccgttaaa ttcaatgat cgttttgaa ttttacgaa aagatttcg ttagatgct tatttgcaa gaaccggat cctaacaat ccggccaac tatactcta catgcagtt ctagtacat tcaggcgac aatcacggt ggtcattat gttgttttt ataaatcct agaggtgat ggcaagtgg tgtaaattt gacgatgat gttgtctcc aaatgtacg aaacaagaa gcaatcgaa cataactac ggcggacat gatgaggat atgaacatg acagtgaaa cattgtaca aatgcgtac atgttggta tacatcaga gattcagag ttgcataac gtattacaa gaagtaacc gatgctgat ataccaagt gaattagct gatcgtttg gctgaagaa aaacgaatg gaacaagtg agacgaaaa gaacgcaac gaagcccat ctctatatg actatcaat gttttgctt gaagatagt tttgatgga catcagggt aatgattta tacgatcct gatcgtcca ctatttcgt gtttttaaa atcaaaaag atggcacta gtgagcgaa atgatggat atgttcgcc gatgcgttt aaatatcca actgaacaa ataagacca tggccattt agtcaacga tcaaatcag acaatgagg ccttgtatg ttagatcta gacactgag tcgcataaa gctattata gattcagct gaaaaccag aatccttgg actatattt ctcgagttg ttgccacct gattcgggc ttaactgcg ttaccaaat tttgacaaa gagaccgat gttttgttg tttttcaaa atgtatgat ccgaaacag aagaaaata cattattgt ggacatagt tacttaccg gttactagt aaattagga gatttgata ccgatgttg aatgagagg gcagggttt ccgccggat acggagttg gtcttgtat gaggagatt aggcctaat atgattgag aaaattact aattatagt gatccgttg gaaaaggtt ttggacgag ttgatggat ggcgatata atcctgttc gagaaagaa gaacgtgag gagttttct gacttgcca acctgtatc gactacttc aaagaccta tattatcgt gtcgaggta acatttgtc gacaaatgt acaccgaac gatcccggt tttacaatg gaattatct caaagaatg acttatgat cagttggca cgtgctgta gctcagagg gtcggtacc gatccttac ttattacaa ttttttaaa tgtcaaaat tacaaagat agtcctgga catccttta aggtgcact tttgagggc accttgaaa gatctctta gttttttcc aagccaaag gcgcctaag aaaatcttt tatcaacag ttgagtatt cgtgttaac gaattagaa aataaaaaa caatttaaa tgtctttat gtgggaccg aatgtgttt gaagagaag gagttaatt ttgtatccg aataagaga gggacggta agtgatttg ttggaagag gcgaagaaa caaatcgag tttggtgaa ggaagtaca gggaaatta aggtttacc gaagttagt tgtaataaa gtagcactg ggtcccaaa gaagatacg ccgctagat catttggta ataaatgca gcaaaggtt tatagaatt gaacaagtg ccaagagat gagttgcac ataaacgag gacgaaatg cttatttct tgtgcgcat tttcaaaag gaagttttt tcgactttt ggctcccct ttcttattt aaaattaaa caaggggag ccttttacc aaggtcaaa gaacgtatt caaaagcga cttggtgtt cctgataag gaattcgaa aagtacaag ttttctatc gttgctatg ggccgacaa caggtcctt caagacgac gaatatgta gttaattta gccgatttt agaccactt ccgaatcaa gctgggagt cccaaacca tggttgggc ctggatcat atgaacaag gccccgaaa agatcacgt tttaattat ttagaaaaa gccatcaaa atttacaat tgagagact gagtgagga agcttattt tcacaacct tgcactttt cggctctgc agtattgcc aattgatcg tgatttccc taaagaatc gataaatgt aaggtgcgg ctgctttgt catttggtg ctttgtgtg agcacaagt cttctgctg tgtgacacc attttgtat ataaaatat atatttgtt ctgtctgtc tatttagaa tatattcgc gctaaatgc tgaatgcta gctgtttat ttacttcgg tttcgagtt attttcttt gaattaatt gaattaact gtttgtaga tttttccgg taatgagac ggtttgaat catgttgaa aataattat tatttgaac taaatcatt aagggagct gcgattttt ttacattaa atttataaa ttgtgatgt tggttgtgt aacattttg tttttaata agcatgaaa tatatgcaa ctgattttt cactgcgtt ctactatgt ttctcaagc tgtaaaaat tatatactt tattgttat ttgaaactt tatgtaaac acattttat aatagaata aagaactaa agtatta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
