Status
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Gene information
General information
TC005316 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14508658..14514473
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC005316
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00826

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg present - Number of animals showing the phenotype: 4

    tropharium irregular - Number of animals showing the phenotype: 4
    egg chamber mostly not present - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    Pupal morphology
    antenna position irregular - Number of animals showing the phenotype: 5

    wings shortened - Number of animals showing the phenotype: 5
    wings not closed - Number of animals showing the phenotype: 5

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005316 RA
      MNHVTGQYR RNDDTSAEV EEMETQEVT TTDSTDTGG 
GEMDQQNGE VIIGPQPDP GMTVQDADM EEDESRSEA 
TFRYTVQQF SKLKDSALS PACYVRNLP WKIMVMPRN 
SHGQDRTAQ RSLGFFLQC NGESESSSW SCYAVAELR 
LLSVRPDVE PFSRKIQHL FYSKENDWG FSHFMAWNE 
VLDPEKGYI KDDAITLEV HVVADAPHG VSWDSKKHT 
GYVGLKNQG ATCYMNSLL QTLYFTNQL RKAVYKMPT 
ESDDSTKSV ALALQRVFH ELQFCDKPV GTKKLTKSF 
GWETLDSFM QHDVQEFLR VLLDKLESK MKGTCVEGT 
VPKLFEGKM ISYIRCKNV DYSSTRSET FYDIQLNIK 
GKKNIDESF KDYIAKETL DGDNKYDAG EHGLQDAEK 
GVIFSAFPP VLHLHLMRF QYDPVTDCS VKFNDRFEF 
YEKISLDAY LQEPDPNNP ANYTLHAVL VHSGDNHGG 
HYVVFINPR GDGKWCKFD DDVVSKCTK QEAIEHNYG 
GHDEDMNMT VKHCTNAYM LVYIRDSEL HNVLQEVTD 
ADIPSELAD RLAEEKRME QVRRKERNE AHLYMTINV 
LLEDSFDGH QGNDLYDPD RPLFRVFKI KKMALVSEM 
MDMFADAFK YPTEQIRPW PFSQRSNQT MRPCMLDLD 
TESHKAIID SAENQNPWT IFLELLPPD SGLTALPNF 
DKETDVLLF FKMYDPKQK KIHYCGHSY LPVTSKLGD 
LIPMLNERA GFPPDTELV LYEEIRPNM IEKITNYSD 
PLEKVLDEL MDGDIILFE KEEREEFSD LPTCIDYFK 
DLYYRVEVT FVDKCTPND PGFTMELSQ RMTYDQLAR 
AVAQRVGTD PYLLQFFKC QNYKDSPGH PLRCTFEGT 
LKDLLVFSK PKAPKKIFY QQLSIRVNE LENKKQFKC 
LYVGPNVFE EKELILYPN KRGTVSDLL EEAKKQIEF 
GEGSTGKLR FTEVSCNKV ALGPKEDTP LDHLVINAA 
KVYRIEQVP RDELHINED EMLISCAHF QKEVFSTFG 
SPFLFKIKQ GEPFTKVKE RIQKRLGVP DKEFEKYKF 
SIVAMGRQQ VLQDDEYVV NLADFRPLP NQAGSPKPW 
LGLDHMNKA PKRSRFNYL EKAIKIYN
    CDS sequence
    • >TC005316 RA
      atgaaccac gttacgggc cagtacaga cgcaacgac 
gacacttcc gccgaagtg gaggagatg gaaacacag 
gaagtcacc accaccgat tctacggac actgggggc 
ggtgaaatg gaccaacag aacggcgaa gtcatcatc 
gggccacag cccgaccct ggaatgaca gttcaagac 
gcagacatg gaagaggac gaatcgcgt tcagaagcc 
acctttcgc tacactgtc caacagttt agcaaactg 
aaagactct gctctgtct ccggcatgt tacgtacgt 
aacctccct tggaaaatc atggttatg ccccggaac 
agtcatgga caggatcgg actgcacag cgctctttg 
ggctttttc cttcagtgt aacggcgaa agcgagtcc 
tcttcgtgg tcttgctat gccgttgct gagctgaga 
ctgctctca gttcgacca gatgttgag cctttctcg 
cgaaaaatt caacatttg ttctattcg aaggaaaat 
gattgggga ttttcacat tttatggcg tggaatgag 
gtcttagac ccggagaaa ggctatatt aaagatgac 
gcgattact ttggaagta catgtggtg gctgatgcc 
ccacatgga gtctcatgg gacagtaaa aaacataca 
ggttacgtg ggtttaaaa aatcaagga gcgacctgt 
tacatgaac tcacttctt caaacttta tattttaca 
aatcagtta cgaaaagcc gtttataaa atgccaacc 
gaatcagat gattcgact aaatctgta gcacttgct 
ctacagcga gtctttcac gaactacaa ttctgcgat 
aaaccagta ggtacgaag aaattgacg aaaagtttc 
ggttgggaa acgttagat tcatttatg caacatgat 
gtgcaggaa tttctcagg gtactttta gataaactt 
gaaagtaag atgaaggga acttgtgtg gagggcacc 
gttccgaaa cttttcgaa ggtaaaatg atttcgtac 
attcggtgt aagaacgtg gattattcg agcacacgt 
tcggagacg ttctatgat attcagttg aatattaag 
gggaagaaa aatatcgat gagtcgttt aaggattac 
atcgcaaag gagactcta gatggcgat aataaatac 
gacgcaggt gaacatggg ttgcaagat gctgaaaaa 
ggggtgatt ttttcggct tttcctcct gttttgcac 
ttgcatttg atgcggttt caatacgat cccgtcact 
gactgttcc gttaaattc aatgatcgt tttgaattt 
tacgaaaag atttcgtta gatgcttat ttgcaagaa 
ccggatcct aacaatccg gccaactat actctacat 
gcagttcta gtacattca ggcgacaat cacggtggt 
cattatgtt gtttttata aatcctaga ggtgatggc 
aagtggtgt aaatttgac gatgatgtt gtctccaaa 
tgtacgaaa caagaagca atcgaacat aactacggc 
ggacatgat gaggatatg aacatgaca gtgaaacat 
tgtacaaat gcgtacatg ttggtatac atcagagat 
tcagagttg cataacgta ttacaagaa gtaaccgat 
gctgatata ccaagtgaa ttagctgat cgtttggct 
gaagaaaaa cgaatggaa caagtgaga cgaaaagaa 
cgcaacgaa gcccatctc tatatgact atcaatgtt 
ttgcttgaa gatagtttt gatggacat cagggtaat 
gatttatac gatcctgat cgtccacta tttcgtgtt 
tttaaaatc aaaaagatg gcactagtg agcgaaatg 
atggatatg ttcgccgat gcgtttaaa tatccaact 
gaacaaata agaccatgg ccatttagt caacgatca 
aatcagaca atgaggcct tgtatgtta gatctagac 
actgagtcg cataaagct attatagat tcagctgaa 
aaccagaat ccttggact atatttctc gagttgttg 
ccacctgat tcgggctta actgcgtta ccaaatttt 
gacaaagag accgatgtt ttgttgttt ttcaaaatg 
tatgatccg aaacagaag aaaatacat tattgtgga 
catagttac ttaccggtt actagtaaa ttaggagat 
ttgataccg atgttgaat gagagggca gggtttccg 
ccggatacg gagttggtc ttgtatgag gagattagg 
cctaatatg attgagaaa attactaat tatagtgat 
ccgttggaa aaggttttg gacgagttg atggatggc 
gatataatc ctgttcgag aaagaagaa cgtgaggag 
ttttctgac ttgccaacc tgtatcgac tacttcaaa 
gacctatat tatcgtgtc gaggtaaca tttgtcgac 
aaatgtaca ccgaacgat cccggtttt acaatggaa 
ttatctcaa agaatgact tatgatcag ttggcacgt 
gctgtagct cagagggtc ggtaccgat ccttactta 
ttacaattt tttaaatgt caaaattac aaagatagt 
cctggacat cctttaagg tgcactttt gagggcacc 
ttgaaagat ctcttagtt ttttccaag ccaaaggcg 
cctaagaaa atcttttat caacagttg agtattcgt 
gttaacgaa ttagaaaat aaaaaacaa tttaaatgt 
ctttatgtg ggaccgaat gtgtttgaa gagaaggag 
ttaattttg tatccgaat aagagaggg acggtaagt 
gatttgttg gaagaggcg aagaaacaa atcgagttt 
ggtgaagga agtacaggg aaattaagg tttaccgaa 
gttagttgt aataaagta gcactgggt cccaaagaa 
gatacgccg ctagatcat ttggtaata aatgcagca 
aaggtttat agaattgaa caagtgcca agagatgag 
ttgcacata aacgaggac gaaatgctt atttcttgt 
gcgcatttt caaaaggaa gttttttcg acttttggc 
tcccctttc ttatttaaa attaaacaa ggggagcct 
tttaccaag gtcaaagaa cgtattcaa aagcgactt 
ggtgttcct gataaggaa ttcgaaaag tacaagttt 
tctatcgtt gctatgggc cgacaacag gtccttcaa 
gacgacgaa tatgtagtt aatttagcc gattttaga 
ccacttccg aatcaagct gggagtccc aaaccatgg 
ttgggcctg gatcatatg aacaaggcc ccgaaaaga 
tcacgtttt aattattta gaaaaagcc atcaaaatt 
tacaattga
    mRNA sequence
    • >TC005316 RA
      ttgccagta tttttttcc ttaaaatta attaaagaa 
aaactgtgt tcggtttcg aattgccaa tatgacagt 
tgtgggtgc atttgttta aaattgttg taaattgtg 
taacttgtg gaaagtgcg ttattttgt gccatttcc 
ggtgttttt tgtgaatta gcgcgggaa aattcgtat 
ctgggcccc ccatttctt gcgccctcg tcgcatacg 
catccgcgc agtcgattg cagaaaccg gtgttcgtc 
gtcgagtgt tgtgcccac catcgctat ttcaatgca 
aagaaaatg cctccattt tgtttattt ttctcattg 
aaaagctca attagcgtt tccaaataa aatcgagtg 
agtgtttca agtgtgaaa agtccgtca cgtgtcaca 
actctctat atatgtgtg ttgtgtgca gccctctct 
cagagggta caaaaacca gcaagaatt ggtcgcgtc 
acgagtaag cactttgtc aaattgact acggtctct 
ggcactaaa aataatacc cagaataac cacggtggt 
aactgttgc gttgcacag tcggacaaa gaccttttg 
aatagcaac aaaaagaac aaaggtgtg ttcgggttc 
gcgaaagac agaggggct gtcaagacg ccagcgctc 
aatatgaac cacgttacg ggccagtac agacgcaac 
gacgacact tccgccgaa gtggaggag atggaaaca 
caggaagtc accaccacc gattctacg gacactggg 
ggcggtgaa atggaccaa cagaacggc gaagtcatc 
atcgggcca cagcccgac cctggaatg acagttcaa 
gacgcagac atggaagag gacgaatcg cgttcagaa 
gccaccttt cgctacact gtccaacag tttagcaaa 
ctgaaagac tctgctctg tctccggca tgttacgta 
cgtaacctc ccttggaaa atcatggtt atgccccgg 
aacagtcat ggacaggat cggactgca cagcgctct 
ttgggcttt ttccttcag tgtaacggc gaaagcgag 
tcctcttcg tggtcttgc tatgccgtt gctgagctg 
agactgctc tcagttcga ccagatgtt gagcctttc 
tcgcgaaaa attcaacat ttgttctat tcgaaggaa 
aatgattgg ggattttca cattttatg gcgtggaat 
gaggtctta gacccggag aaaggctat attaaagat 
gacgcgatt actttggaa gtacatgtg gtggctgat 
gccccacat ggagtctca tgggacagt aaaaaacat 
acaggttac gtgggttta aaaaatcaa ggagcgacc 
tgttacatg aactcactt cttcaaact ttatatttt 
acaaatcag ttacgaaaa gccgtttat aaaatgcca 
accgaatca gatgattcg actaaatct gtagcactt 
gctctacag cgagtcttt cacgaacta caattctgc 
gataaacca gtaggtacg aagaaattg acgaaaagt 
ttcggttgg gaaacgtta gattcattt atgcaacat 
gatgtgcag gaatttctc agggtactt ttagataaa 
cttgaaagt aagatgaag ggaacttgt gtggagggc 
accgttccg aaacttttc gaaggtaaa atgatttcg 
tacattcgg tgtaagaac gtggattat tcgagcaca 
cgttcggag acgttctat gatattcag ttgaatatt 
aaggggaag aaaaatatc gatgagtcg tttaaggat 
tacatcgca aaggagact ctagatggc gataataaa 
tacgacgca ggtgaacat gggttgcaa gatgctgaa 
aaaggggtg attttttcg gcttttcct cctgttttg 
cacttgcat ttgatgcgg tttcaatac gatcccgtc 
actgactgt tccgttaaa ttcaatgat cgttttgaa 
ttttacgaa aagatttcg ttagatgct tatttgcaa 
gaaccggat cctaacaat ccggccaac tatactcta 
catgcagtt ctagtacat tcaggcgac aatcacggt 
ggtcattat gttgttttt ataaatcct agaggtgat 
ggcaagtgg tgtaaattt gacgatgat gttgtctcc 
aaatgtacg aaacaagaa gcaatcgaa cataactac 
ggcggacat gatgaggat atgaacatg acagtgaaa 
cattgtaca aatgcgtac atgttggta tacatcaga 
gattcagag ttgcataac gtattacaa gaagtaacc 
gatgctgat ataccaagt gaattagct gatcgtttg 
gctgaagaa aaacgaatg gaacaagtg agacgaaaa 
gaacgcaac gaagcccat ctctatatg actatcaat 
gttttgctt gaagatagt tttgatgga catcagggt 
aatgattta tacgatcct gatcgtcca ctatttcgt 
gtttttaaa atcaaaaag atggcacta gtgagcgaa 
atgatggat atgttcgcc gatgcgttt aaatatcca 
actgaacaa ataagacca tggccattt agtcaacga 
tcaaatcag acaatgagg ccttgtatg ttagatcta 
gacactgag tcgcataaa gctattata gattcagct 
gaaaaccag aatccttgg actatattt ctcgagttg 
ttgccacct gattcgggc ttaactgcg ttaccaaat 
tttgacaaa gagaccgat gttttgttg tttttcaaa 
atgtatgat ccgaaacag aagaaaata cattattgt 
ggacatagt tacttaccg gttactagt aaattagga 
gatttgata ccgatgttg aatgagagg gcagggttt 
ccgccggat acggagttg gtcttgtat gaggagatt 
aggcctaat atgattgag aaaattact aattatagt 
gatccgttg gaaaaggtt ttggacgag ttgatggat 
ggcgatata atcctgttc gagaaagaa gaacgtgag 
gagttttct gacttgcca acctgtatc gactacttc 
aaagaccta tattatcgt gtcgaggta acatttgtc 
gacaaatgt acaccgaac gatcccggt tttacaatg 
gaattatct caaagaatg acttatgat cagttggca 
cgtgctgta gctcagagg gtcggtacc gatccttac 
ttattacaa ttttttaaa tgtcaaaat tacaaagat 
agtcctgga catccttta aggtgcact tttgagggc 
accttgaaa gatctctta gttttttcc aagccaaag 
gcgcctaag aaaatcttt tatcaacag ttgagtatt 
cgtgttaac gaattagaa aataaaaaa caatttaaa 
tgtctttat gtgggaccg aatgtgttt gaagagaag 
gagttaatt ttgtatccg aataagaga gggacggta 
agtgatttg ttggaagag gcgaagaaa caaatcgag 
tttggtgaa ggaagtaca gggaaatta aggtttacc 
gaagttagt tgtaataaa gtagcactg ggtcccaaa 
gaagatacg ccgctagat catttggta ataaatgca 
gcaaaggtt tatagaatt gaacaagtg ccaagagat 
gagttgcac ataaacgag gacgaaatg cttatttct 
tgtgcgcat tttcaaaag gaagttttt tcgactttt 
ggctcccct ttcttattt aaaattaaa caaggggag 
ccttttacc aaggtcaaa gaacgtatt caaaagcga 
cttggtgtt cctgataag gaattcgaa aagtacaag 
ttttctatc gttgctatg ggccgacaa caggtcctt 
caagacgac gaatatgta gttaattta gccgatttt 
agaccactt ccgaatcaa gctgggagt cccaaacca 
tggttgggc ctggatcat atgaacaag gccccgaaa 
agatcacgt tttaattat ttagaaaaa gccatcaaa 
atttacaat tgagagact gagtgagga agcttattt 
tcacaacct tgcactttt cggctctgc agtattgcc 
aattgatcg tgatttccc taaagaatc gataaatgt 
aaggtgcgg ctgctttgt catttggtg ctttgtgtg 
agcacaagt cttctgctg tgtgacacc attttgtat 
ataaaatat atatttgtt ctgtctgtc tatttagaa 
tatattcgc gctaaatgc tgaatgcta gctgtttat 
ttacttcgg tttcgagtt attttcttt gaattaatt 
gaattaact gtttgtaga tttttccgg taatgagac 
ggtttgaat catgttgaa aataattat tatttgaac 
taaatcatt aagggagct gcgattttt ttacattaa 
atttataaa ttgtgatgt tggttgtgt aacattttg 
tttttaata agcatgaaa tatatgcaa ctgattttt 
cactgcgtt ctactatgt ttctcaagc tgtaaaaat 
tatatactt tattgttat ttgaaactt tatgtaaac 
acattttat aatagaata aagaactaa agtatta
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