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Gene information
General information
TC005313 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid58: 1357637..1359388
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005313
    iBeetle screen iB_00825
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005313 RA
      MADAAFTRN EIISLVVRL TLVSAVTFI SIKWLMNQV 
      DPTNKSKKK ARKKAQEQL KRLAGSGHA PLVIENLSD 
      YEMMIAAHL IHPQDINVT WGNIAGLDD MIQELRETV 
      ILPIQRKEL FADSQLTTA PRGVLLHGP PGCGKTLIA 
      KATAKEAGT RFINLDLSI LTDKWYGES QKLAAAVFT 
      LAVKLQPCI IFIDEIDSF LRSRNTTDH EATAMMKAQ 
      FMSLWDGLI TDPNCTVIV MGATNRPQD LDRAILRRM 
      PATFHISMP NPVQRKKIL QLTLENEPV AHDVDIDRL 
      ARLTDGFSG SDLRELCRN GSVYRVRDY MKTHSDTVL 
      DSSEDEYQD ALRPITMED LMVSLNKMK ESKMHCGAL 
      PTNRIELD
    CDS sequence
    • >TC005313 RA
      atggccgat gccgctttt acccgtaat gagatcata 
      agtttagtt gtgaggtta accttggtt tctgctgta 
      acgttcatt agtataaaa tggttaatg aaccaagtc 
      gatcccact aataagagt aaaaagaag gctcggaaa 
      aaggcacag gagcagttg aaaaggtta gctggtagc 
      gggcatgct cctttagtt atcgaaaac ttgtccgac 
      tatgaaatg atgatagcg gcgcacttg atacacccc 
      caggacatt aacgtgact tggggcaac atcgccggc 
      ttggacgac atgatccag gagctacgc gagactgtt 
      atacttcca atccaacgc aaggagctg tttgcggac 
      tcgcaactc accactgcc cccagaggt gtgttgtta 
      catgggccc ccaggttgt ggcaaaacg ctcatcgct 
      aaggctaca gccaaagag gctgggacc cgatttatc 
      aacttggac ttatcaatc ctcactgat aagtggtat 
      ggggagagc cagaaatta gcagctgct gtgttcacc 
      cttgctgtt aaacttcaa ccctgtatt atttttatt 
      gacgaaatt gactcgttt ctaaggtcg aggaatacc 
      accgaccac gaggcgact gctatgatg aaagcccaa 
      tttatgtca ttgtgggat ggtttgatc actgatccg 
      aattgtact gttatagtc atgggggct acaaatcgc 
      ccccaggat ttagaccgg gcgatttta cgccgaatg 
      ccggccaca ttccatatt tcaatgcca aaccctgta 
      caaagaaaa aagattcta caactgact cttgagaat 
      gaacccgtt gcgcatgat gttgacata gatagacta 
      gcgaggctc actgatggc ttctcaggt tcggatttg 
      agagagttg tgtcggaac gggtcagtt tatcgtgtt 
      agggattac atgaaaacg cacagtgac acggttttg 
      gattcgtcg gaagatgag tatcaagac gcattacgg 
      ccaatcaca atggaagat ttaatggtt tcgttaaat 
      aaaatgaaa gaatcgaaa atgcattgt ggggcttta 
      ccgacgaat cggatcgag ttggattag
    mRNA sequence
    • >TC005313 RA
      cgccacttt tggtaacaa agtcgtgcc aacccaccg 
      tgtgacagt taacaacaa gtgacactg tttttacat 
      ttctttgta atagtggga tgccccggt tttaaaatc 
      gtcaatggt agtgtaatt ccaactttc gcgataaac 
      tgaaatggc cgatgccgc ttttacccg taatgagat 
      cataagttt agttgtgag gttaacctt ggtttctgc 
      tgtaacgtt cattagtat aaaatggtt aatgaacca 
      agtcgatcc cactaataa gagtaaaaa gaaggctcg 
      gaaaaaggc acaggagca gttgaaaag gttagctgg 
      tagcgggca tgctccttt agttatcga aaacttgtc 
      cgactatga aatgatgat agcggcgca cttgataca 
      cccccagga cattaacgt gacttgggg caacatcgc 
      cggcttgga cgacatgat ccaggagct acgcgagac 
      tgttatact tccaatcca acgcaagga gctgtttgc 
      ggactcgca actcaccac tgcccccag aggtgtgtt 
      gttacatgg gcccccagg ttgtggcaa aacgctcat 
      cgctaaggc tacagccaa agaggctgg gacccgatt 
      tatcaactt ggacttatc aatcctcac tgataagtg 
      gtatgggga gagccagaa attagcagc tgctgtgtt 
      cacccttgc tgttaaact tcaaccctg tattatttt 
      tattgacga aattgactc gtttctaag gtcgaggaa 
      taccaccga ccacgaggc gactgctat gatgaaagc 
      ccaatttat gtcattgtg ggatggttt gatcactga 
      tccgaattg tactgttat agtcatggg ggctacaaa 
      tcgccccca ggatttaga ccgggcgat tttacgccg 
      aatgccggc cacattcca tatttcaat gccaaaccc 
      tgtacaaag aaaaaagat tctacaact gactcttga 
      gaatgaacc cgttgcgca tgatgttga catagatag 
      actagcgag gctcactga tggcttctc aggttcgga 
      tttgagaga gttgtgtcg gaacgggtc agtttatcg 
      tgttaggga ttacatgaa aacgcacag tgacacggt 
      tttggattc gtcggaaga tgagtatca agacgcatt 
      acggccaat cacaatgga agatttaat ggtttcgtt 
      aaataaaat gaaagaatc gaaaatgca ttgtggggc 
      tttaccgac gaatcggat cgagttgga ttagcagcc 
      gtggtttca gtacttagc acccattat agataattt 
      tgacgacac tgtagaaat gtatcaaag tttttcaaa 
      taatttcaa acattgtgt taattatca gaaaaagct 
      ttagtttta tttagccca acgaggctg tgccacgag 
      tattgtgtt gggttattt gtgtatata gtttaggga 
      aatgagtcg ctctcttca gagtacaaa cactttcta 
      ttcagcgtt gatatttta agcatgtcg cattcataa 
      tatgacttt atgtgtagt ttacaaaga ttttaaagt 
      gtgatgatc aacggtttt cactccatg ttgaagtca 
      tgcgtcgca tgtttacag tgttcttca ttttttttt 
      atgtgaaaa ggttaaaga ataaaaatt acggacgtt 
      a