General information | |||
TC005310 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 14556507..14559613 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC005310 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00824 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
appendages partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
- >TC005310 RA
MNESGNHED GEIRATRFR NLPSVNSLS QEELIVTQP ENTELLSSI RRRVQTSSE KIETGSLNV LSAKYESLD YDTCENYLL LDEERKKGY RFIVKKSLA RWFIFLLIG VVTALIACA IDISIEELS QLKYASLSK YVDEYVTQG KLHIPYFFW VLYNIIPVL IGSTLVAYV EPIAAGSGI PQVKCYLNG VKIPRVVRI KTLFVKSVG VVCSVVGGL AGGKEGPMI HSGAVVAAG LSQGKSTTF RRDFKIFKF FREDHEKRD FVSGGAAAG VSAAFGAPV GGVLFSLEE GTSFWNQSL TWRTFFASV ISTFTLNVV LSTYHGVPG DLSYPGLLN LGKFENFSY KVYELPIFL VMGALGGLS GALWNHINY KLSVFRMRY IRKRWLKVI EACIVAAVS ATLGFLMMF LLNDCKPLG QDPTKYPTQ LYCQDGQYN VLASIWFQT PEASVRSLF HDPPSTHNA TSLAFFVLV YFFLSSWTF GLASSNGLF IPTLLTGAA WGRLISVGL FRVIPDAVL IHPGKYALI GAAAQLGGV VRMTISLTV IIMETTGNI SFALPLILT LIAAKWTGD FFNEGIYDT LIQLSGVPL LPWEPPPLV HNIYASEVM SHPVVTLKC VENVGHIVE LLKLTTYNG FPVVDPPLT DQSEVTTYG RIRGLVLRS QLIVILKKK IFNENSDFW EDINAGIFR DEYPRYPTI EQVSVSEVE KTYSIDLRP FMNPSPYTV LHSASLPRM FRLFRALGL RHLPIVNDT NEVIGMVTR KDLARYRVW RHRGRMGVE ELLISKEI
- >TC005310 RA
atgaacgaa agtgggaat catgaggat ggtgaaatt cgagccacc agatttagg aatttgcca tcggtcaat tcgttgagt caagaagaa ctgattgtg acacaacct gaaaatacc gaattgtta tcgtcaatc agacggagg gtacaaact agttctgaa aaaattgaa acagggtct ttgaacgtg ttatcggcg aaatatgaa agccttgat tacgacacg tgtgagaat tatttactt ttggatgaa gagagaaaa aaaggttat agatttatt gtgaaaaaa agtttagcg agatggttt attttcctc ttaattgga gtggtaacc gcgttaatt gcttgtgcc atagatata tcaatagaa gaattatca caactgaaa tatgcctca ttaagcaaa tatgtggac gaatacgtc acacagggg aaactccac atcccctac ttcttctgg gtgttatac aacataatc ccagtccta ataggctca actctagtt gcttacgtt gagcctatt gctgctggt agtggaatt cctcaagtg aaatgttac ctcaatggg gttaaaata ccccgcgtc gtacgaata aaaacacta tttgtaaaa agtgtaggc gttgtgtgt tcagttgtg ggtggttta gctgggggc aaagaagga ccaatgatt cattcagga gctgtcgtt gcagcggga ctttctcaa gggaaaagt acaacgttt aggcgagat ttcaaaata tttaaattt tttcgggaa gatcacgaa aaacgggac tttgtctct gggggcgcc gctgcggga gtttcggca gctttcgga gcaccagtt ggtggagtg ctttttagt ttggaagaa ggtacaagc ttttggaat cagagccta acatggcgc acttttttc gcttccgtg ataagcacc tttactcta aacgtagtt ttatcaact tatcacgga gttcccggt gatttaagc tatccagga cttttgaat ttaggcaaa tttgaaaac tttagttat aaagtgtac gaattacca attttcctc gtaatgggg gctttaggt ggattatcg ggggcgtta tggaatcac atcaattac aaattatca gtttttcga atgagatat atacgaaaa agatggtta aaagtaatt gaagcttgt attgtcgca gctgttagt gccacttta ggtttcctg atgatgttt ttactgaat gattgcaag cctttggga caagatccc acgaaatat ccaactcag ttatactgt caagatggc caatacaat gttttggca agcatttgg tttcaaact cctgaagcc tcagtccgt tctcttttt cacgatccc ccaagtacg cacaacgcc acctctctg gcctttttc gtcctagtt tatttcttt ttatcgtca tggactttt ggtcttgcc tcttctaat ggtcttttt ataccgact ctcctaacg ggggctgct tggggtcgg cttatttca gtcggacta tttcgagta atccccgac gctgttctc attcatccg gggaaatac gccctgatt ggtgcagcc gctcaatta ggaggcgta gtcagaatg acaattagt ttgacagtg attataatg gaaacgaca ggaaacatt tcctttgct ttgcctcta attctgaca ttaattgcg gcaaaatgg actggtgat tttttcaat gagggaatt tatgacaca cttatacaa ctcagtggt gttccgtta ctcccatgg gaacccccg ccactagtt cacaatatc tacgccagt gaggtgatg tcgcatccg gtggtcaca ctgaagtgt gttgagaat gtggggcat attgttgaa ttgttgaaa ttaacaact tataacgga tttccggtt gttgatccg ccactaact gatcaatcg gaagttacc acgtatggg agaataaga gggttagtt ttgaggtca caattaatt gttattttg aaaaagaag atttttaac gaaaattcg gacttttgg gaggatatt aatgcggga atctttcga gatgagtat ccccgatat ccaacaata gagcaagtg agtgtttcc gaagttgaa aaaacttat tctattgat ctgaggccg tttatgaat ccgtcacca tatacggtg cttcattcg gcatcacta cctcgaatg tttagatta ttccgagct ctcggttta cgacatttg ccgattgtt aatgacaca aatgaggtt ataggaatg gtaacaagg aaagattta gctcgttat agagtctgg agacatcgg ggtcgcatg ggggtagaa gaattatta atttccaaa gaaatatga
- >TC005310 RA
tgacgttta atttttcgg agaatgcaa aataatatg aatacgttt ttatcgcag atgattcat ttcgtaatc aaatcggga cacaattca aagttataa tgcgcgtta ttttctaat ctgggagaa tgaacgaaa gtgggaatc atgaggatg gtgaaattc gagccacca gatttagga atttgccat cggtcaatt cgttgagtc aagaagaac tgattgtga cacaacctg aaaataccg aattgttat cgtcaatca gacggaggg tacaaacta gttctgaaa aaattgaaa cagggtctt tgaacgtgt tatcggcga aatatgaaa gccttgatt acgacacgt gtgagaatt atttacttt tggatgaag agagaaaaa aaggttata gatttattg tgaaaaaaa gtttagcga gatggttta ttttcctct taattggag tggtaaccg cgttaattg cttgtgcca tagatatat caatagaag aattatcac aactgaaat atgcctcat taagcaaat atgtggacg aatacgtca cacagggga aactccaca tcccctact tcttctggg tgttataca acataatcc cagtcctaa taggctcaa ctctagttg cttacgttg agcctattg ctgctggta gtggaattc ctcaagtga aatgttacc tcaatgggg ttaaaatac cccgcgtcg tacgaataa aaacactat ttgtaaaaa gtgtaggcg ttgtgtgtt cagttgtgg gtggtttag ctgggggca aagaaggac caatgattc attcaggag ctgtcgttg cagcgggac tttctcaag ggaaaagta caacgttta ggcgagatt tcaaaatat ttaaatttt ttcgggaag atcacgaaa aacgggact ttgtctctg ggggcgccg ctgcgggag tttcggcag ctttcggag caccagttg gtggagtgc tttttagtt tggaagaag gtacaagct tttggaatc agagcctaa catggcgca cttttttcg cttccgtga taagcacct ttactctaa acgtagttt tatcaactt atcacggag ttcccggtg atttaagct atccaggac ttttgaatt taggcaaat ttgaaaact ttagttata aagtgtacg aattaccaa ttttcctcg taatggggg ctttaggtg gattatcgg gggcgttat ggaatcaca tcaattaca aattatcag tttttcgaa tgagatata tacgaaaaa gatggttaa aagtaattg aagcttgta ttgtcgcag ctgttagtg ccactttag gtttcctga tgatgtttt tactgaatg attgcaagc ctttgggac aagatccca cgaaatatc caactcagt tatactgtc aagatggcc aatacaatg ttttggcaa gcatttggt ttcaaactc ctgaagcct cagtccgtt ctctttttc acgatcccc caagtacgc acaacgcca cctctctgg cctttttcg tcctagttt atttctttt tatcgtcat ggacttttg gtcttgcct cttctaatg gtcttttta taccgactc tcctaacgg gggctgctt ggggtcggc ttatttcag tcggactat ttcgagtaa tccccgacg ctgttctca ttcatccgg ggaaatacg ccctgattg gtgcagccg ctcaattag gaggcgtag tcagaatga caattagtt tgacagtga ttataatgg aaacgacag gaaacattt cctttgctt tgcctctaa ttctgacat taattgcgg caaaatgga ctggtgatt ttttcaatg agggaattt atgacacac ttatacaac tcagtggtg ttccgttac tcccatggg aacccccgc cactagttc acaatatct acgccagtg aggtgatgt cgcatccgg tggtcacac tgaagtgtg ttgagaatg tggggcata ttgttgaat tgttgaaat taacaactt ataacggat ttccggttg ttgatccgc cactaactg atcaatcgg aagttacca cgtatggga gaataagag ggttagttt tgaggtcac aattaattg ttattttga aaaagaaga tttttaacg aaaattcgg acttttggg aggatatta atgcgggaa tctttcgag atgagtatc cccgatatc caacaatag agcaagtga gtgtttccg aagttgaaa aaacttatt ctattgatc tgaggccgt ttatgaatc cgtcaccat atacggtgc ttcattcgg catcactac ctcgaatgt ttagattat tccgagctc tcggtttac gacatttgc cgattgtta atgacacaa atgaggtta taggaatgg taacaagga aagatttag ctcgttata gagtctgga gacatcggg gtcgcatgg gggtagaag aattattaa tttccaaag aaatatgac cgcttacag tattatgat atgataata tagtttcac gaataatta tgttgtcgc ttttgacaa aagaatttt atttattgt taattaaag actttaccc attt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
