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Gene information
General information
TC005308 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid58: 1379181..1381358
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005308
    iBeetle screen iB_00823
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005308 RA
      MGRNILKSS VQNASFSII FQILFRCIT FVLNAFIIR 
      TVGQEVLGI MNVRLLLLE STILFLSHE PLMKACLTD 
      TKSHNWAQV INQIWLSVP MTAVLSLVL VYIWINILS 
      PTGDNYVSQ YRLGCYSIA LSCIIEQMT QSVVLVAQS 
      YCFVKLKVV LETIYIVSR TVIFVCTVV KHPNDAINA 
      FSIAQLGSA VILCLSYYG FFFWYIRRL NLVKKGTTV 
      KSSLFTDMN DFPFSSIME FFPGIMENG ERILNQDLC 
      LLTISFAKQ SIIKQILTE GERYVMTVS PVLTFSQQS 
      MYDIVNNLG SLAARFIFR PIEESAYFY FTQMIKRDE 
      PVDKQDQRY ISESANVLS HLCNIVTCI GLTVVVFGQ 
      SYSHTLLYL YGGKKLVEN TLPVTLLRF HSFAIVLLA 
      INGVTEGYV FATMNNKQL DRYNYIMVI FSVSFLVIS 
      YVLTNALGP VGFILANCF NMLARIIHS LIYINKKYK 
      GTVYKPLEG LIPSKKFLL TLAVSGVVT KISDSLISS 
      IIVHILIGA VFFLASFSI WAIDNKSIL KLGYNKYKR 
      RMSTKTD
    CDS sequence
    • >TC005308 RA
      atggggcga aatatactg aaaagtagc gtccagaac 
      gcatcattc agcattatt tttcaaatt ctcttccga 
      tgcattact tttgtgtta aatgctttc ataattcga 
      actgttggc caggaagtg ttgggcata atgaacgtt 
      cggttgctt ctcctcgaa tcaaccatt ctgttcctg 
      tcacatgag cccctaatg aaagcgtgt ttgacagac 
      accaagtcg cacaattgg gcgcaagtt atcaaccaa 
      atttggctt tctgttccg atgacggca gttttaagt 
      ttggttttg gtgtacatt tggatcaat atcctaagc 
      cccactggt gataattac gtcagtcaa tatagactt 
      ggttgttac tctattgct ctgtcatgt attatcgag 
      caaatgacc caaagtgtg gtacttgtg gctcaaagt 
      tactgtttt gttaaatta aaagttgtc cttgagaca 
      atttatatt gtctctagg acggttatt tttgtttgc 
      acggttgta aagcaccca aatgatgct attaatgcg 
      ttttcaatt gcgcagcta gggtcagct gttatttta 
      tgcctgtct tattacggt tttttcttt tggtatatt 
      aggcgattg aatctagta aaaaaaggg acaactgtt 
      aaaagtagt cttttcacc gacatgaac gactttccg 
      ttctcttcc ataatggag tttttccct ggaattatg 
      gaaaatggc gaaagaatt ctaaaccaa gacttgtgt 
      cttttaaca atcagtttt gccaaacaa tcaatcatc 
      aaacaaatt ttaacggaa ggggagcgc tacgtaatg 
      actgtctct ccagtgctg acctttagt caacagtca 
      atgtacgac attgtaaat aatttaggc agcttggct 
      gcacgattt atttttcga ccaattgag gaaagtgct 
      tatttttat tttacgcaa atgatcaaa cgagacgaa 
      cctgtggac aaacaggac caaagatac atttccgaa 
      tcagcaaat gtcttaagc cacttgtgc aacattgtg 
      acatgtata ggcctcact gttgttgtt tttggccaa 
      agttatagt cacacttta ctttacctt tatgggggc 
      aaaaaacta gttgaaaac actttgcct gtgacgcta 
      ttacgtttt cacagtttt gcgattgtt ctactcgcc 
      ataaatggg gtcacagag ggctatgtt ttcgcaaca 
      atgaataac aaacagtta gataggtac aattacatt 
      atggtgata ttttcagtc agttttttg gtgatttct 
      tacgtcctg acaaatgca ttaggacca gttggattt 
      attttagca aattgcttt aatatgtta gcgcgtatt 
      atccacagt ctcatttat atcaataaa aaatacaaa 
      gggacagtt tacaaacct cttgaaggt ttaatccca 
      agcaaaaaa ttcttacta acgctggct gtgtcagga 
      gttgttact aaaatatca gattcgttg atttcctca 
      atcattgtt catatttta ataggggct gtatttttc 
      cttgcaagt tttagcatc tgggccatt gataataaa 
      tcaatcctc aagctgggt tacaacaaa tacaaacgc 
      cgaatgtcc acaaaaact gattaa
    mRNA sequence
    • >TC005308 RA
      cataaccta aaaatgtaa acacgaata taaattaca 
      aaatggggc gaaatatac tgaaaagta gcgtccaga 
      acgcatcat tcagcatta tttttcaaa ttctcttcc 
      gatgcatta cttttgtgt taaatgctt tcataattc 
      gaactgttg gccaggaag tgttgggca taatgaacg 
      ttcggttgc ttctcctcg aatcaacca ttctgttcc 
      tgtcacatg agcccctaa tgaaagcgt gtttgacag 
      acaccaagt cgcacaatt gggcgcaag ttatcaacc 
      aaatttggc tttctgttc cgatgacgg cagttttaa 
      gtttggttt tggtgtaca tttggatca atatcctaa 
      gccccactg gtgataatt acgtcagtc aatatagac 
      ttggttgtt actctattg ctctgtcat gtattatcg 
      agcaaatga cccaaagtg tggtacttg tggctcaaa 
      gttactgtt ttgttaaat taaaagttg tccttgaga 
      caatttata ttgtctcta ggacggtta tttttgttt 
      gcacggttg taaagcacc caaatgatg ctattaatg 
      cgttttcaa ttgcgcagc tagggtcag ctgttattt 
      tatgcctgt cttattacg gttttttct tttggtata 
      ttaggcgat tgaatctag taaaaaaag ggacaactg 
      ttaaaagta gtcttttca ccgacatga acgactttc 
      cgttctctt ccataatgg agtttttcc ctggaatta 
      tggaaaatg gcgaaagaa ttctaaacc aagacttgt 
      gtcttttaa caatcagtt ttgccaaac aatcaatca 
      tcaaacaaa ttttaacgg aaggggagc gctacgtaa 
      tgactgtct ctccagtgc tgaccttta gtcaacagt 
      caatgtacg acattgtaa ataatttag gcagcttgg 
      ctgcacgat ttatttttc gaccaattg aggaaagtg 
      cttattttt attttacgc aaatgatca aacgagacg 
      aacctgtgg acaaacagg accaaagat acatttccg 
      aatcagcaa atgtcttaa gccacttgt gcaacattg 
      tgacatgta taggcctca ctgttgttg tttttggcc 
      aaagttata gtcacactt tactttacc tttatgggg 
      gcaaaaaac tagttgaaa acactttgc ctgtgacgc 
      tattacgtt ttcacagtt ttgcgattg ttctactcg 
      ccataaatg gggtcacag agggctatg ttttcgcaa 
      caatgaata acaaacagt tagataggt acaattaca 
      ttatggtga tattttcag tcagttttt tggtgattt 
      cttacgtcc tgacaaatg cattaggac cagttggat 
      ttattttag caaattgct ttaatatgt tagcgcgta 
      ttatccaca gtctcattt atatcaata aaaaataca 
      aagggacag tttacaaac ctcttgaag gtttaatcc 
      caagcaaaa aattcttac taacgctgg ctgtgtcag 
      gagttgtta ctaaaatat cagattcgt tgatttcct 
      caatcattg ttcatattt taatagggg ctgtatttt 
      tccttgcaa gttttagca tctgggcca ttgataata 
      aatcaatcc tcaagctgg gttacaaca aatacaaac 
      gccgaatgt ccacaaaaa ctgattaat ttaataaaa 
      gagaagaaa agtcaacac ttattgtat tttattctt 
      ataaacagc agcagtaac agtccctgt gcttgtagg 
      tctgaatat gagacagtc cttgttatc actttcaag 
      tttaatgat aggatcccc actgtgagg ctcaagttt 
      agcggcctg ctcggctat cgcagcgat taggccggc 
      gctctcggt cgaggtttt ccccttcgc ccccaggca 
      caaaccttg acggtcaac gaaaacaca accaaagac 
      ccccgctct gctcaccct accaaaaca attg