Status
Please wait ...
Gene information
General information
TC005305 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14574422..14576533
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005305
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00822

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005305 RA
      MLAKTGLLV VSNPTQIGK ILPSIQKRV KNTLYIQLL 
      SALSEPFGN FYTNVFTSW PKYSKTLYG IYSQAATHC 
      ENLDVRVLL SGLKYQTVS QIQTKSNID LIIFDRIHS 
      KTDIDNFIK TKVANIASE YNVVTLDSA GDRIEDFIC 
      DDDESIYNH TVLGGTFDR LHTAHKLLL TEAALRSRR 
      KVTVGVTEE SMLPGKILW ELIENLDVR IQNVENFLN 
      DICAELDYN VVPISDPFG PSITDPTME MIVVSEETV 
      KGSQKINEI RLEKQLNPL DVHSVKLID EPYPNLNEE 
      RKISSSTGR IRLLGTLLR PVAKKNIPN KPYVIGLTG 
      GVASGKSGV ANWLIQHEA EIINCDLIG HEVYKSGRP 
      CHTKIVDCF GDSVLTPEG EVDRKVLGS IVFSDSSAL 
      KKLNGLVWP AIADEVQKM ITNSKNPVV VVEAAVLLT 
      AGWQSFCHE VWSTLVPRS EAIKRLMAR NKLTEEQAI 
      ARINSQPSN KAYVQEAHV VLCPLWDVE FTGMQVKKA 
      WDLLQDRLL
    CDS sequence
    • >TC005305 RA
      atgttggca aaaactggc cttttagtt gtttcaaat 
      cctacacaa atcggtaaa attttgcct tcgattcaa 
      aaacgtgta aaaaacaca ctctacata caattatta 
      tcggctctg agtgaaccg tttggtaat ttttacaca 
      aacgtgttt acttcgtgg ccaaagtat agtaagaca 
      ctttatggg atttattca caggctgca acgcattgc 
      gaaaacctc gatgttcgt gtccttttg tctggtttg 
      aaataccaa actgtgagc caaatccaa accaaatcc 
      aacatagat ttaataatt ttcgataga attcacagt 
      aaaaccgat attgataat ttcattaaa acaaaggtt 
      gctaacatt gcctctgaa tataatgtt gtgacattg 
      gattcagca ggcgatagg atcgaagat ttcatttgt 
      gacgatgat gagagcatt tacaatcac actgttcta 
      gggggcact tttgataga ctccacacg gcccacaaa 
      ttgctttta actgaagca gcattgagg agcagacgt 
      aaagtaact gtgggtgtc actgaagaa tccatgttg 
      ccaggtaag attttatgg gaactcatt gaaaatctt 
      gatgtgcga atccaaaat gtcgagaat tttttaaat 
      gacatttgc gccgaattg gattataac gtcgtgcca 
      atttcggat ccgtttggt ccttcaatc acagatcca 
      acgatggaa atgatcgta gtgagcgaa gaaactgta 
      aaaggcagt caaaaaatc aatgaaata cgtttagaa 
      aaacaattg aatccactt gacgttcac tccgtcaaa 
      ttaatcgac gagccgtat ccgaacttg aacgaagaa 
      agaaagata agttcgagt acgggccgt atacgactt 
      ttggggaca cttttacga ccagtagca aaaaaaaat 
      atccctaac aaaccctac gttattggt ttaaccggc 
      ggtgttgcc agtggcaaa tctggtgtt gccaactgg 
      ttgatacaa cacgaagct gaaataatc aactgtgat 
      ttaatcgga cacgaagtc tacaaatct ggtaggccg 
      tgtcataca aaaatcgtc gattgtttc ggtgattcg 
      gtcttaacg ccagaaggc gaagtcgac cgaaaggtt 
      ctcggatcg atcgttttc tccgattcg agtgctttg 
      aaaaaattg aacggatta gtgtggcca gcaatcgca 
      gatgaggtt caaaaaatg atcacaaat agcaaaaat 
      ccggttgtg gttgttgaa gctgctgtg cttttaacg 
      gccggttgg caaagtttt tgtcacgag gtttggagt 
      acgttagtg ccccgtagt gaggctatt aaacggctc 
      atggcaaga aataagttg acagaagaa caggccatt 
      gcacgaatt aacagtcaa ccgagtaat aaagcatac 
      gttcaagag gcacatgtt gtgttgtgt cctttatgg 
      gacgtggaa tttacaggg atgcaggtc aagaaggca 
      tgggatttg ctgcaggat agattgttg tga
    mRNA sequence
    • >TC005305 RA
      atagtgttt attgaggag tacgttcct aaaaatttt 
      gatttggca cctaaaatt ctcttaaaa cgaattaaa 
      aacaaaaaa aaaacactt aaaccaaca acacttggg 
      aaatctgtg tggacttta agattagca aaaaaaaca 
      aattacaat tctcccatt agactgcct tcatgtgac 
      aaattacct aacctcaaa atttataag agataagta 
      taaaactga cgcaaattt tttgtttct gctcattcg 
      cgttatgta aatatatac tttggtcaa ggttttgca 
      tagtgaaaa tgttggcaa aaactggcc ttttagttg 
      tttcaaatc ctacacaaa tcggtaaaa ttttgcctt 
      cgattcaaa aacgtgtaa aaaacacac tctacatac 
      aattattat cggctctga gtgaaccgt ttggtaatt 
      tttacacaa acgtgttta cttcgtggc caaagtata 
      gtaagacac tttatggga tttattcac aggctgcaa 
      cgcattgcg aaaacctcg atgttcgtg tccttttgt 
      ctggtttga aataccaaa ctgtgagcc aaatccaaa 
      ccaaatcca acatagatt taataattt tcgatagaa 
      ttcacagta aaaccgata ttgataatt tcattaaaa 
      caaaggttg ctaacattg cctctgaat ataatgttg 
      tgacattgg attcagcag gcgatagga tcgaagatt 
      tcatttgtg acgatgatg agagcattt acaatcaca 
      ctgttctag ggggcactt ttgatagac tccacacgg 
      cccacaaat tgcttttaa ctgaagcag cattgagga 
      gcagacgta aagtaactg tgggtgtca ctgaagaat 
      ccatgttgc caggtaaga ttttatggg aactcattg 
      aaaatcttg atgtgcgaa tccaaaatg tcgagaatt 
      ttttaaatg acatttgcg ccgaattgg attataacg 
      tcgtgccaa tttcggatc cgtttggtc cttcaatca 
      cagatccaa cgatggaaa tgatcgtag tgagcgaag 
      aaactgtaa aaggcagtc aaaaaatca atgaaatac 
      gtttagaaa aacaattga atccacttg acgttcact 
      ccgtcaaat taatcgacg agccgtatc cgaacttga 
      acgaagaaa gaaagataa gttcgagta cgggccgta 
      tacgacttt tggggacac ttttacgac cagtagcaa 
      aaaaaaata tccctaaca aaccctacg ttattggtt 
      taaccggcg gtgttgcca gtggcaaat ctggtgttg 
      ccaactggt tgatacaac acgaagctg aaataatca 
      actgtgatt taatcggac acgaagtct acaaatctg 
      gtaggccgt gtcatacaa aaatcgtcg attgtttcg 
      gtgattcgg tcttaacgc cagaaggcg aagtcgacc 
      gaaaggttc tcggatcga tcgttttct ccgattcga 
      gtgctttga aaaaattga acggattag tgtggccag 
      caatcgcag atgaggttc aaaaaatga tcacaaata 
      gcaaaaatc cggttgtgg ttgttgaag ctgctgtgc 
      ttttaacgg ccggttggc aaagttttt gtcacgagg 
      tttggagta cgttagtgc cccgtagtg aggctatta 
      aacggctca tggcaagaa ataagttga cagaagaac 
      aggccattg cacgaatta acagtcaac cgagtaata 
      aagcatacg ttcaagagg cacatgttg tgttgtgtc 
      ctttatggg acgtggaat ttacaggga tgcaggtca 
      agaaggcat gggatttgc tgcaggata gattgttgt 
      gattgatta ttttatttt aaatttatg taaaaacca 
      agtgatgga aatatttta ataaataat aatgtttgg 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY