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Gene information
General information
TC005277 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450498.1: 14141..18679
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005277
    iBeetle screen iB_00820

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005277 RA
      MSSHRNEQD KWINPSKRE YVPVEGFTL TDQFKQAKN 
      QIDNFEVSD NDIWISTFP KSGTTWTQE MVWLIFNNL 
      DFEKAKQNL NDRSPFLEI STLIDYQNL MKTCPDIQI 
      PESRLDSIK FVKNQKGPK VIKTHLPWE LLPKQIQNG 
      VKKPKIIYV ARNPKDVCV SFFNHEKLI SGYSGTFDE 
      FCELFLDGK VLYAPYWHH VLTYWKMRN TPNFLFLKY 
      EDMKRDLSK VIQKVSEFL ERPLNDEQV EILLEHLSF 
      EKMKQNPAV NKEDMIDIF KKHNLTNSD GQFFRSGKI 
      GDYKVTMSS GMIKRFDEW IKRNTEGSD YVV
    CDS sequence
    • >TC005277 RA
      atgagttct cacagaaat gaacaagac aagtggatt 
      aatccttct aagagagaa tatgtccca gtagaaggt 
      tttactctt actgatcaa tttaaacaa gcaaaaaac 
      caaattgat aactttgaa gttagcgat aatgatatt 
      tggatctca acctttcct aagtcaggt actacgtgg 
      actcaagaa atggtgtgg ttgattttt aataacttg 
      gattttgaa aaagcaaaa caaaattta aatgaccga 
      tctccattt ttagaaata agcacccta attgattat 
      caaaattta atgaaaact tgtccagat attcaaata 
      ccagaaagt cgactagat tcaataaaa tttgtcaaa 
      aatcaaaaa ggaccaaaa gtaattaag acacattta 
      ccttgggag ttgttacca aaacaaatt caaaatggt 
      gttaaaaaa ccaaaaatt atttatgtc gctcgaaat 
      ccaaaagat gtttgtgtt tcttttttt aatcacgag 
      aagttgata agcggttac agcggcact ttcgacgaa 
      ttttgtgaa ctgtttctt gacggaaaa gtattatat 
      gcaccatac tggcatcac gttttgacg tattggaaa 
      atgaggaat acaccaaat ttcctattc ctaaaatat 
      gaagatatg aaaagagat ttaagcaaa gtgattcaa 
      aaggtttct gaatttttg gaacgtcct ttgaatgat 
      gaacaggtt gaaatttta cttgaacac ttaagtttt 
      gagaaaatg aagcaaaat cctgcagtg aataaagaa 
      gacatgatt gatatattc aaaaaacat aatttaaca 
      aattccgat ggacagttt ttcagatct ggaaaaatc 
      ggagattat aaggttaca atgtcttca ggaatgata 
      aagcgattt gatgaatgg attaaaaga aatacagaa 
      ggaagcgat tatgttgtt taa
    mRNA sequence
    • >TC005277 RA
      gttcattta taaaaaaat tcactttaa ctctcgaat 
      agataaaac atgagttct cacagaaat gaacaagac 
      aagtggatt aatccttct aagagagaa tatgtccca 
      gtagaaggt tttactctt actgatcaa tttaaacaa 
      gcaaaaaac caaattgat aactttgaa gttagcgat 
      aatgatatt tggatctca acctttcct aagtcaggt 
      actacgtgg actcaagaa atggtgtgg ttgattttt 
      aataacttg gattttgaa aaagcaaaa caaaattta 
      aatgaccga tctccattt ttagaaata agcacccta 
      attgattat caaaattta atgaaaact tgtccagat 
      attcaaata ccagaaagt cgactagat tcaataaaa 
      tttgtcaaa aatcaaaaa ggaccaaaa gtaattaag 
      acacattta ccttgggag ttgttacca aaacaaatt 
      caaaatggt gttaaaaaa ccaaaaatt atttatgtc 
      gctcgaaat ccaaaagat gtttgtgtt tcttttttt 
      aatcacgag aagttgata agcggttac agcggcact 
      ttcgacgaa ttttgtgaa ctgtttctt gacggaaaa 
      gtattatat gcaccatac tggcatcac gttttgacg 
      tattggaaa atgaggaat acaccaaat ttcctattc 
      ctaaaatat gaagatatg aaaagagat ttaagcaaa 
      gtgattcaa aaggtttct gaatttttg gaacgtcct 
      ttgaatgat gaacaggtt gaaatttta cttgaacac 
      ttaagtttt gagaaaatg aagcaaaat cctgcagtg 
      aataaagaa gacatgatt gatatattc aaaaaacat 
      aatttaaca aattccgat ggacagttt ttcagatct 
      ggaaaaatc ggagattat aaggttaca atgtcttca 
      ggaatgata aagcgattt gatgaatgg attaaaaga 
      aatacagaa ggaagcgat tatgttgtt taaaataat 
      tattatata atttttaat cgaggcata aaatagtta 
      ttgtataca ctactaatg tttatctgt tgggtgtaa 
      aacatggta gccataaag tttttttat aatcttatt 
      actgttcag ttgaaagca caataagca tatttaatt 
      ttaaacaca aaattgagt tacttgaaa caatgtcag 
      aaaacaagg atttccaca ttttaaaga ctcgattta 
      gaataaacg attgcctaa cctttgttt gaaatttcc 
      ctttggcaa ggtttaaac aatgtcctc tcatgcgag 
      cagatgttt tgagtggga aaatatgct taataagag 
      aacatgggt tattagtta ttaattata tacacattg 
      attaattat atacactta cgagtataa taagctgca 
      acaagagat ctggtgtct aaggtaccg aaggttttg 
      cttataata taaataaaa gaataattt ctaaaatat 
      tttgctctt caagtagtc taatgtgta tgtaatcaa 
      aaatagtgg tgcatgggg ccctggggc aacatctca 
      gtggtaaag gtggctctg aaaaaaaga tattatctt 
      cactttgat tacaatcgg tccatttgc ggatattag 
      gtaggataa ggatatatt tatagtttt gaagtttct 
      gaatcttgg atttttgaa tctggactg gcaattgtt 
      gaaaattat ggatttgac tggaaaatt ttaacaatt 
      gaattctgc ataattaca ctcatcgtt acatgcact 
      tcaagggta aaaactatt tgactgcac tttgaacaa 
      cttttaacg tgaaggagc gtgactgta tcaaaaaag 
      tacttccgc aaaactgta caaagtgta aaagtgtaa 
      gctaaaaac gcgcaaata ttcaaagta ttattcatc 
      gttctttta caaaaatcg gtcatttta ttctgcaat 
      gttcgattc agagaggca ccgtgccca tgtatttat 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY