Status
Please wait ...
Gene information
General information
TC005233 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 1803040..1833566
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005233
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00818

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005233 RA
      MLDYFTKLV GNNSGENGR LLNTGNQKV NDSSNVHTV 
      KMSTYERDR LGQRGAGDN AATSSTSGL DRLKNASLN 
      KGMAFTLEE RQILGIHGF LPPRVKTQE EQVEHCRMC 
      LERLDNSLN KYLYLMNLL DRNEKLFYR LVGENVQDI 
      MPLIYTPTV GEACQKFGL VYRRPRGLF ITIHDKGHV 
      YDILKNWPE SDIRVIVVT DGERILGLG DLGACGMGI 
      PIGKLSLYT ALAGIKPHQ CLPITLDVG TNTEKMLND 
      PLYIGLKQN RVRGQQYDD FIEEFMQSV VRRWGQNTL 
      IQFEDFGNA NAFRLLGKY RDSYCTFND DIQGTASVA 
      VAGILASLR ATKTRLSDN TLVFQGAGE ASLGIAALC 
      VMAMKAEGT PEHEARKRI WMVDSKGLI VKNRPDGGI 
      TEHKEPFAQ DHAPIKTLA EVVQQVKPT ILIGAAAVG 
      GAFTPEILR EMAKNCKKP VIFALSNPT SKAECTAEQ 
      AYENTDGTC IFASGSPFD PVTYKGRTY YPGQGNNSY 
      IFPGVGLAA ICVGMRTIG EDVFLISAE TLANLVSEA 
      DLEKGSLYP PLSTIQEVS VKIATRLGE YAYKKGFAT 
      VCPQPDDME AFIRAQMYN TDYIPAVPC TYPFP
    CDS sequence
    • >TC005233 RA
      atgcttgat tatttcaca aaactggtt ggaaacaat 
      tcaggagaa aacggacga ctactgaac accggcaat 
      cagaaagta aacgactcg tcgaacgtg cacacagtg 
      aaaatgtcg acgtacgaa agagatcga ctgggccaa 
      cgaggagcc ggagacaat gctgctaca agttcaacg 
      agtggtctc gatcgacta aagaatgcc agcttaaat 
      aagggaatg gcttttact ttggaggaa cgacaaatt 
      ttgggcatc cacggtttt ctccctcca cgagtaaaa 
      acacaagaa gaacaagta gaacattgt cgtatgtgt 
      ctggagcgt cttgataat agtctcaac aaatacctt 
      tacctaatg aatctcctt gatcgcaac gagaaattg 
      ttttatcgt ctcgttgga gaaaatgtc caagatata 
      atgcctctg atctacact cctactgta ggtgaagca 
      tgccaaaaa ttcggtttg gtttatcgg cgacctaga 
      ggcttgttc ataaccatt cacgataaa ggacatgtt 
      tatgatatt ttgaagaac tggcccgaa tccgacatc 
      agggtaatc gtcgtaaca gacggcgaa cgcatcctg 
      ggtctgggt gacttgggt gcctgtggt atgggcata 
      cccatcggc aaactctcc ctctacacg gctttagcc 
      ggaattaaa ccgcaccaa tgtctgcca ataactcta 
      gatgttgga acaaacacc gaaaaaatg ctcaacgat 
      ccactttac atcggccta aaacaaaac cgtgtcaga 
      ggccaacaa tacgacgat ttcatcgaa gaattcatg 
      caatcagtt gtaagacgt tggggccaa aacactctc 
      attcaattt gaagatttt ggaaacgca aatgcgttc 
      cgtctttta ggcaaatac agggatagt tattgcacc 
      ttcaatgat gatattcaa ggcactgca agtgttgca 
      gttgccgga attttggca tcacttcgt gccacgaaa 
      actcgattg tctgacaat actttggtg tttcaagga 
      gctggtgaa gcttcgctt ggaattgct gcactttgt 
      gtgatggca atgaaagcc gaaggaaca ccggaacat 
      gaagcacgg aaacgaatc tggatggtt gattccaaa 
      ggtttgatt gttaaaaat cgaccagac gggggaatc 
      accgaacat aaagaaccc ttcgctcag gatcatgca 
      ccaattaaa acacttgcc gaagttgtg caacaagtc 
      aaaccgacc attttgatt ggagctgcg gcagttggc 
      ggtgcgttc acgccagag attttgaga gagatggct 
      aagaattgt aaaaaaccc gtgattttt gcattgagt 
      aatccgact tcgaaggct gaatgtacg gctgaacaa 
      gcgtatgaa aatacagat gggacttgt atttttgca 
      agtggaagt ccctttgat cctgttact tataaggga 
      cgcacttat tatccagga caagggaat aattcgtat 
      attttcccg ggtgttggt ttggctgcg atttgtgtg 
      ggaatgcgt acgattgga gaagatgta tttttgatt 
      tctgctgag accctcgct aatttggta tcagaggct 
      gatttagag aaaggaagt ctctaccca cctctgtct 
      acaattcag gaagtgtct gtgaaaatt gcaacacgt 
      cttggagaa tatgcttac aagaaaggt tttgcaacg 
      gtttgccca caacctgac gacatggaa gctttcata 
      agggcacaa atgtacaac acagactac atccctgca 
      gtcccttgc acttatccc ttcccctaa
    mRNA sequence
    • >TC005233 RA
      gaacaccaa acctttgag tgaaaacca cagttcttt 
      tattatttg gcaataatt acctgttgt tttcatcac 
      taatcagat ttgctctgt taattgctt tattcttga 
      ctttattac taaaattat gtggcggtc gtgaacgat 
      aggagaaat ttgttacct gggtgctat gccattgaa 
      acaaatcta atccattta ttatattta gtttctata 
      tttgcgcaa caactacaa gatgggaaa cgcggcctg 
      tatgtctgt attgtgtgg agaaaacga cacagccac 
      caaaaaaag aataatttg ataagctcg tattattgg 
      gtgcacaat cacaatcta aaagtcgca aagtacaaa 
      ttgaatatg ctatatata agaagaatc aacagccga 
      aatttaaaa ctaccgaac agttcacac tgttccaaa 
      gtgagtgta gtacgtgcc tatgtgaaa atgcttgat 
      tatttcaca aaactggtt ggaaacaat tcaggagaa 
      aacggacga ctactgaac accggcaat cagaaagta 
      aacgactcg tcgaacgtg cacacagtg aaaatgtcg 
      acgtacgaa agagatcga ctgggccaa cgaggagcc 
      ggagacaat gctgctaca agttcaacg agtggtctc 
      gatcgacta aagaatgcc agcttaaat aagggaatg 
      gcttttact ttggaggaa cgacaaatt ttgggcatc 
      cacggtttt ctccctcca cgagtaaaa acacaagaa 
      gaacaagta gaacattgt cgtatgtgt ctggagcgt 
      cttgataat agtctcaac aaatacctt tacctaatg 
      aatctcctt gatcgcaac gagaaattg ttttatcgt 
      ctcgttgga gaaaatgtc caagatata atgcctctg 
      atctacact cctactgta ggtgaagca tgccaaaaa 
      ttcggtttg gtttatcgg cgacctaga ggcttgttc 
      ataaccatt cacgataaa ggacatgtt tatgatatt 
      ttgaagaac tggcccgaa tccgacatc agggtaatc 
      gtcgtaaca gacggcgaa cgcatcctg ggtctgggt 
      gacttgggt gcctgtggt atgggcata cccatcggc 
      aaactctcc ctctacacg gctttagcc ggaattaaa 
      ccgcaccaa tgtctgcca ataactcta gatgttgga 
      acaaacacc gaaaaaatg ctcaacgat ccactttac 
      atcggccta aaacaaaac cgtgtcaga ggccaacaa 
      tacgacgat ttcatcgaa gaattcatg caatcagtt 
      gtaagacgt tggggccaa aacactctc attcaattt 
      gaagatttt ggaaacgca aatgcgttc cgtctttta 
      ggcaaatac agggatagt tattgcacc ttcaatgat 
      gatattcaa ggcactgca agtgttgca gttgccgga 
      attttggca tcacttcgt gccacgaaa actcgattg 
      tctgacaat actttggtg tttcaagga gctggtgaa 
      gcttcgctt ggaattgct gcactttgt gtgatggca 
      atgaaagcc gaaggaaca ccggaacat gaagcacgg 
      aaacgaatc tggatggtt gattccaaa ggtttgatt 
      gttaaaaat cgaccagac gggggaatc accgaacat 
      aaagaaccc ttcgctcag gatcatgca ccaattaaa 
      acacttgcc gaagttgtg caacaagtc aaaccgacc 
      attttgatt ggagctgcg gcagttggc ggtgcgttc 
      acgccagag attttgaga gagatggct aagaattgt 
      aaaaaaccc gtgattttt gcattgagt aatccgact 
      tcgaaggct gaatgtacg gctgaacaa gcgtatgaa 
      aatacagat gggacttgt atttttgca agtggaagt 
      ccctttgat cctgttact tataaggga cgcacttat 
      tatccagga caagggaat aattcgtat attttcccg 
      ggtgttggt ttggctgcg atttgtgtg ggaatgcgt 
      acgattgga gaagatgta tttttgatt tctgctgag 
      accctcgct aatttggta tcagaggct gatttagag 
      aaaggaagt ctctaccca cctctgtct acaattcag 
      gaagtgtct gtgaaaatt gcaacacgt cttggagaa 
      tatgcttac aagaaaggt tttgcaacg gtttgccca 
      caacctgac gacatggaa gctttcata agggcacaa 
      atgtacaac acagactac atccctgca gtcccttgc 
      acttatccc ttcccctaa atgtaattt attagcgaa 
      attatttat atgatgctt aaaactgtt gctggtggt 
      aggaaatga actgtcgac cagtttatt ttgtaaatt 
      tatttttaa aataaatgt aaattattt gtttttata 
      atagtgttt tcattcaat aaaaatatc actctaaat 
      atgtaatca gtttaatct tcaggttct tccatagta 
      actacaatt tctaccagt gctaaatac attatacaa 
      aaaaggaca taaacaacc tctatcaaa atcagttga 
      acattccca gactagatg cttaatatg tagtaacag 
      ctgaatcat agtatttta tcacaaaaa gtataaaaa 
      cgtttggca aaaattaac aaaacattt caccttgac 
      taaagcaaa ttcttcagt gaattggtg ttttcaatt 
      tttgccttc agttcgtgc accggagga ctaaaagac 
      tagaaggca aaatgagat ttttttaat gtttctaaa 
      aacgtgtat tttatttga acataactc caaattggt 
      gaacaaagt gtttatccc aagataact tttaacagt 
      catttcaaa aacaaaata aaatttagc tactatttt 
      tgtgcaaga ttcttttaa gacgctatc tgactaaaa 
      ttccatcat ccacaaaga ttttcaaaa caaaggtct 
      actacacct tttttcaat cgtatttca gattggaaa 
      tatacagtg tgacccagg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY