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Gene information
General information
TC005232 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 1781370..1799790
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005232
    iBeetle screen iB_00817

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005232 RA
      MFALIPRSL VTNVSSRSA VSLSGQKAT VQEAVKWLI 
      DSKKDYHTV SGDTITPSK VMGIDHLRD PRLNKGLAF 
      TLEERQALG IHGLQPARF KTQEEQLEL CRISVMRYQ 
      ENLNKYLYM TELHDRNEK LFFRLLSEN IEMLMPIVY 
      TPTVGLACQ RFGLIYRRP RGLFITIND RGHVYDVLK 
      NWPESDVRA IVVTDGERI LGLGDLGAC GMGIPVGKL 
      ALYTALAGI KPHHCLPIV LDVGTNNQT LLEDPLYVG 
      LRQKRLSGP EYDSFVDEF MQAAVKRYG QNVLIQFED 
      FGNHNAFRY LDKYRGVYC TFNDDIQGT AGVAVSGLL 
      ASARVTGKK ISENKFLFL GAGEAAIGI ADLCVKAMQ 
      VEGLSLEEA RGKIWMMDI DGLLAKGRP EGKLDGHKA 
      YYAKEHKPV KDLASVVNE VKPSVLIGA SAASGAFTP 
      EILKSMASF NDRPIIFAL SNPTDRAEC TAEQAYNNT 
      DGRCIFSSG SPFPPVTYK GTTFYPGQG NNAYIFPGV 
      ALGVILARI HHIKEELFL LAAQAVADH VNDSDIQKG 
      SLYPPLSCI RECSVDIAT RILDYSYQE GIATVYPEP 
      QDKKAYVMQ HLYNYNYES ALPTTWPWP NAPEIKTRP 
      IEPTKLMA
    CDS sequence
    • >TC005232 RA
      atgttcgct ctcattccc cggtccctt gtaacgaac 
      gtgtcatca cgatcggcg gttagttta tcgggacag 
      aaagcaacc gttcaagaa gcagtcaaa tggctcata 
      gattcgaaa aaagattac cacactgtg tcaggagat 
      acaatcacg ccttcaaaa gtcatgggt atcgaccat 
      cttcgtgat cctcgtctc aacaagggt ctggctttc 
      actttggaa gaacgtcaa gctttgggt atccatggt 
      ctacaacct gcccgattt aaaacacaa gaagaacaa 
      ctggaatta tgccgaatt tctgtgatg cgttatcag 
      gagaatctc aacaaatat ctgtatatg acggaattg 
      catgatcgt aatgagaaa ttatttttc cgtttgtta 
      tccgaaaat atcgaaatg ttgatgccg attgtttac 
      acacctaca gttggttta gcctgtcag aggtttgga 
      ttgatctat cggagacca agaggactc tttatcaca 
      atcaatgac agaggacat gtctacgac gttctgaaa 
      aactggcca gaatcagac gtccgagca atcgtcgta 
      acagacggt gaacgtata ttgggtttg ggcgattta 
      ggagcatgt ggtatgggt attcctgtt ggaaaactc 
      gctctatac actgcactt gccggcatc aaaccgcat 
      cattgtctt ccgatcgtc ctagatgtc ggaaccaac 
      aaccaaaca ctgttggaa gaccctctc tatgtaggt 
      ctacgccag aaaagactt tcaggacct gaatacgac 
      tcatttgtt gatgaattc atgcaagct gcagtcaag 
      cgttacggt caaaacgtc ctcatccaa ttcgaagat 
      ttcggaaat cacaacgct ttccgttat ttggataaa 
      taccgaggt gtctattgc accttcaat gacgatatt 
      cagggtact gcaggtgtt gctgtttcg ggccttttg 
      gcttctgca agagttacc ggcaagaaa atatccgaa 
      aataagttt ctgtttttg ggagctggt gaagctgcc 
      attggtatt gctgacctg tgtgttaaa gcaatgcaa 
      gtcgaagga ttgtcactg gaagaagcg agaggaaaa 
      atctggatg atggatatt gatggttta ttggctaaa 
      ggacgtccg gaagggaaa ctagacgga cataaggcg 
      tactatgcg aaagaacat aaaccagtc aaagatttg 
      gcttcagtt gtaaatgaa gttaaacca tcggtttta 
      attggtgcg tctgctgca agtggcgct tttacgcct 
      gaaattttg aaaagtatg gcgtcgttt aacgataga 
      ccgattatt tttgccttg agtaatcct accgacagg 
      gcggagtgt acagcagaa caggcctat aataatact 
      gatggtcgt tgcatcttc tcaagcggt tctccgttc 
      cctcctgtc acctacaaa ggtaccact ttttaccca 
      ggtcaaggc aacaacgct tacattttc cctggagtt 
      gctttgggt gttatctta gccagaatt caccacatt 
      aaggaagaa ttgttctta ttggctgct caagccgtt 
      gctgatcat gtgaacgac tccgatatc caaaagggt 
      agtttatat ccacctctc agttgcatc agggaatgt 
      tctgtcgat attgctacg agaatttta gattactcc 
      taccaagaa ggaattgca accgtatat ccggaacca 
      caggacaaa aaagcatac gttatgcaa cacctttat 
      aattacaat tacgaatcg gcattgcct actacatgg 
      ccatggcct aatgcaccc gaaattaag acaaggccc 
      atcgaaccg acaaagttg atggcttaa
    mRNA sequence
    • >TC005232 RA
      ttcaataag ataagaaac acaacaaag tgcgataaa 
      tttcgcgca gtcgcgtgc tgtgtgtac tgcgagagg 
      ccgactaca atcagctgt gagcaacta ccggggttc 
      taaaaatgt tcgctctca ttccccggt cccttgtaa 
      cgaacgtgt catcacgat cggcggtta gtttatcgg 
      gacagaaag caaccgttc aagaagcag tcaaatggc 
      tcatagatt cgaaaaaag attaccaca ctgtgtcag 
      gagatacaa tcacgcctt caaaagtca tgggtatcg 
      accatcttc gtgatcctc gtctcaaca agggtctgg 
      ctttcactt tggaagaac gtcaagctt tgggtatcc 
      atggtctac aacctgccc gatttaaaa cacaagaag 
      aacaactgg aattatgcc gaatttctg tgatgcgtt 
      atcaggaga atctcaaca aatatctgt atatgacgg 
      aattgcatg atcgtaatg agaaattat ttttccgtt 
      tgttatccg aaaatatcg aaatgttga tgccgattg 
      tttacacac ctacagttg gtttagcct gtcagaggt 
      ttggattga tctatcgga gaccaagag gactcttta 
      tcacaatca atgacagag gacatgtct acgacgttc 
      tgaaaaact ggccagaat cagacgtcc gagcaatcg 
      tcgtaacag acggtgaac gtatattgg gtttgggcg 
      atttaggag catgtggta tgggtattc ctgttggaa 
      aactcgctc tatacactg cacttgccg gcatcaaac 
      cgcatcatt gtcttccga tcgtcctag atgtcggaa 
      ccaacaacc aaacactgt tggaagacc ctctctatg 
      taggtctac gccagaaaa gactttcag gacctgaat 
      acgactcat ttgttgatg aattcatgc aagctgcag 
      tcaagcgtt acggtcaaa acgtcctca tccaattcg 
      aagatttcg gaaatcaca acgctttcc gttatttgg 
      ataaatacc gaggtgtct attgcacct tcaatgacg 
      atattcagg gtactgcag gtgttgctg tttcgggcc 
      ttttggctt ctgcaagag ttaccggca agaaaatat 
      ccgaaaata agtttctgt ttttgggag ctggtgaag 
      ctgccattg gtattgctg acctgtgtg ttaaagcaa 
      tgcaagtcg aaggattgt cactggaag aagcgagag 
      gaaaaatct ggatgatgg atattgatg gtttattgg 
      ctaaaggac gtccggaag ggaaactag acggacata 
      aggcgtact atgcgaaag aacataaac cagtcaaag 
      atttggctt cagttgtaa atgaagtta aaccatcgg 
      ttttaattg gtgcgtctg ctgcaagtg gcgctttta 
      cgcctgaaa ttttgaaaa gtatggcgt cgtttaacg 
      atagaccga ttatttttg ccttgagta atcctaccg 
      acagggcgg agtgtacag cagaacagg cctataata 
      atactgatg gtcgttgca tcttctcaa gcggttctc 
      cgttccctc ctgtcacct acaaaggta ccacttttt 
      acccaggtc aaggcaaca acgcttaca ttttccctg 
      gagttgctt tgggtgtta tcttagcca gaattcacc 
      acattaagg aagaattgt tcttattgg ctgctcaag 
      ccgttgctg atcatgtga acgactccg atatccaaa 
      agggtagtt tatatccac ctctcagtt gcatcaggg 
      aatgttctg tcgatattg ctacgagaa ttttagatt 
      actcctacc aagaaggaa ttgcaaccg tatatccgg 
      aaccacagg acaaaaaag catacgtta tgcaacacc 
      tttataatt acaattacg aatcggcat tgcctacta 
      catggccat ggcctaatg cacccgaaa ttaagacaa 
      ggcccatcg aaccgacaa agttgatgg cttaaaatt 
      tgaaaaaca tgtaattta attgatagt aaggcctct 
      gtaactttc tgatttgct tcagatgga tggaaatta 
      aaagttaca aaaggcact tcaataggt aattctttt 
      tccatctgt attttttaa attcgacgt attttaaca 
      taatttatt gttgaatgt ttacaacct aagctttat 
      ttaaaaata tacaatgtt gatcattac tttgttttg 
      cgtgggcac tagaaaata gtgccattt tcagttaat 
      tttgtactt aattttatt tagtattca ttcgatcac 
      agcagcttt cgattaata agtattgtt tttgtgttt 
      cttttttgt atttgctga tctacgagc aatatcgtt 
      gtacgtttt aaacagtat ttctttctg gtcattaaa 
      atgtagaac gttagatta agaccacct gtaataaaa 
      aaacaaaat aaatgttat tgtactgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY