Status
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Gene information
General information
TC034281
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005217,TC005216
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 1749546..1788262
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034281
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00815

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034281 RA
      MSNFDIIEF GFNTADETV NFGTRSLAD GDNLYEAST 
      LATLKPSNG ADANLDPYI NAVVKNGSI YFSIDNSLR 
      IFEDKQCSY VAFSATFSA VVDSFCVSS DCEYLLVCL 
      RNGTVQLID QNNENKCVF TDLCERDED CDRFFINSF 
      VYNLCFVFV TKQGKIYQL DPRHFELKC VHVFNFATN 
      VASFKAPFL LLSGTKLAV LNLESRKSV IDSEDNQFK 
      KIYPLRNNF FALDFEGKL YKICAMTLL IFPINSQHL 
      YRDMLLISD SNEEQVVVT EDEMQQKSL QLLSYPALS 
      KIYGLNVKT DIYLISPEC VNDELLYIS KIVENNSVS 
      ELRFHMVYE TDAEQRLAR LLRKRKYEE AERFAALFH 
      LDASLVNEA KAQDIVDKM TCTSEEIDQ LINLLNLID 
      NDVFKLQSC LGVTCSKFE DVRKILEYG CTLQLKKVD 
      NEAEKMQNS VIDVMARFE TFGALSKRA ELDIQTWQQ 
      FANADLMDE VKSFLQNHE IEEAKIIYS RLDVATIET 
      LTDDKISDI LNILNMLPP ATYQSFLSI FIPVTLSLY 
      PTALPLIVK WTRNKVFSL EQHSKSDFP SNAIILTKK 
      IIALMKMGD VADISFQRQ HILHRDSLS ELLATLEII 
      KTLKEEYRI SVPVQNYLT GPQNVIRIL LNVEMCPND 
      FDRFLRNFL CKYIVDNHM DPNVILLEE IKNLMKYDE 
      RYWVNIISL ILDCINSVE IKLQGVQTI LNCAKVPWS 
      DTFIFWPSL PKYREKLIQ RFDEALHII DNNNADVQH 
      LCLLTETIV KDKLRLQDE TISNFYTVA GSLFKRLNK 
      NSNEFERSF NDTTLDLIK NVYNLRKHF GIRTTALKS 
      HSDKNKIID EILENVRLE FENEKQTID EVFATCHKL 
      SVCLSCDYH DILLRFCEK TGDYNFIVR TAEKFHDRE 
      TSTRNFCLM AILLLRYIG AYKLSLGDS FSDSDRSLV 
      VADSERVVS DDVFCKGLR IATSIVAKA MAKAEPDEL 
      MPCMEVVNW VYVAFHFTG SQESDLSKL LFRNNYRPL 
      QMVSGFHTF SAIKLVFNT FCGFVAHVK STNSAYLTH 
      FKNQPLTEE EFMHELQPL IPILENLSK DGQHYTSFV 
      IVSTLTNCL MQYNPLAKN LITTFDKIL KKKCIPKLL 
      SVVVSSNTI DTDLLNNLL QLCDREDAT KFILNALRM 
      YKRHPRKFE CLTRTGLKI CDHYELTDY KRVMMDSLI 
      TLKWWKRFE GHKIPYDKF FKSKPEQRF ELLINTLDC 
      FHIEWLNEF CKDYKLEPQ TCYLFYLKR VLQNWKPDY 
      EIKSDDTNR RFLIVKNSE VELLAKCRE IMQGLNNEQ 
      ISGVVRTVR NSINTYYYE VFITILDIL CEVDPQESQ 
      FNELAMLQF LKNYQRVSK ASQIEIEQW FTNFPDAQT 
      VDVLSEFRL AFTPLLFTS DIFTIIRPE INLKTYHLW 
      FNTIEILRP HLSKNQICS YVVKHVVAS GVLSKSDET 
      WVLNPKHDD LLRAIDECV NNITDPELA TSVMYHVMT 
      NTCPGLDQV KAASLCYKY ATKYRQLNP DSSAVEKAV 
      TKVEKKYYM YRALHILNV HHLTDSKYI DLLTHPNDL 
      ILALYLDDR ILDDFHCVE INKASEALA ELFQLDIKK 
      IRLKLINSW LDDQALVDF DSSVIESNC SEESCLKRI 
      AYICSGDQV TYWQTVLLE IGQKGESVR CRANALRVF 
      AMISDVETI ETMAEMSYD RLMLFINRL TLVGNLEYL 
      GFGVDVAVL DHTNRQSLL NRLVQQGGN AFAIKTMAL 
      YCMTYQIYG AKYWEFVLN NAVKFSLFT ELKTYVTFL 
      SKSKYQYKG FYINAWQKL IDYYFSNLN CAKNLDQTL 
      LKHFLTIQS CPVLFSLDF NHAFAMCLE VKKAEFAAV 
      LLQYLQKND TYFSNVEKV RNCSKEIMP GLNYLDQNG 
      ILGISNVRE ILHNSV
    CDS sequence
    • >TC034281 RA
      atgtctaat tttgatata attgagttc ggctttaac 
      acagctgat gaaaccgtt aacttcgga actcgaagt 
      ttagcagac ggagacaat ttatacgaa gcctcaact 
      ttagccacc cttaaacca tcaaatggg gctgacgcg 
      aatttggac ccttatatc aacgccgtt gtaaaaaat 
      ggctcaatt tatttcagt attgataat tcgctgcgt 
      atttttgaa gacaaacag tgctcttat gtcgctttt 
      agtgcaact tttagcgct gttgtggac tcgttttgt 
      gtctcgtcc gattgcgaa tatttatta gtttgtctc 
      aggaatggc acagtgcaa ttgattgac caaaacaac 
      gaaaataaa tgtgttttc accgattta tgtgaacgg 
      gatgaggat tgtgatcgc ttctttatc aattctttt 
      gtttacaat ttatgcttt gtttttgtc acaaaacag 
      ggaaaaatt taccaactg gaccccaga cactttgaa 
      ctgaaatgt gttcatgtg tttaatttt gcaacaaat 
      gtggcgagt tttaaagcc ccttttttg ctacttagt 
      ggtacaaaa ttggcggtt ttaaatttg gaaagtcgt 
      aaaagtgtc attgattcc gaggacaat cagtttaag 
      aaaatttac ccattaaga aataatttt tttgccttg 
      gattttgag ggaaaatta tacaagatt tgtgcaatg 
      actcttttg atttttccg attaattcg caacacttg 
      tatagagac atgcttcta atttctgat tcgaatgaa 
      gaacaggtt gttgtgacg gaagatgaa atgcagcaa 
      aaaagtctt cagttactt tcatatcca gccttgagt 
      aaaatatac ggtttgaac gtgaaaacc gacatctat 
      ttaatatct ccagaatgt gtaaacgat gagctttta 
      tacatttct aaaattgtc gagaacaat tcagtgtcg 
      gaattgcgt tttcacatg gtttatgaa actgatgcc 
      gaacagaga cttgcaaga ttgttaaga aaacgcaaa 
      tatgaagaa gctgaacgt tttgctgct cttttccac 
      ttggatgcg agtcttgtg aacgaggca aaagcccaa 
      gatattgtc gataaaatg acttgcact agtgaagaa 
      atcgaccaa cttatcaat cttttgaat ttaatcgac 
      aacgatgtg ttcaaactg cagtcttgt ttgggtgta 
      acttgtagt aaatttgaa gatgttcgc aaaatactc 
      gagtatggt tgtacattg caattgaag aaagtggac 
      aacgaagct gaaaaaatg caaaacagt gtaattgat 
      gttatggca aggtttgag acgtttggg gcgctttca 
      aaaagggct gagttggat atacagact tggcaacaa 
      ttcgcaaac gctgatttg atggatgag gtcaagagt 
      ttcctgcaa aatcatgaa atcgaagaa gccaaaata 
      atttacagt cgcttagac gtcgccact atcgaaacc 
      ctaactgac gacaaaatt tcagacatt ttaaacatc 
      ttaaatatg ctaccacca gctacctat cagtctttc 
      ctatcaatt ttcattcca gtaacactt tcactttac 
      cctacagcc cttcctcta atcgttaaa tggacacgc 
      aataaagtg ttttctttg gagcaacat tcaaagagc 
      gattttcca agcaatgca attatttta acgaaaaaa 
      ataatcgca ttgatgaag atgggcgat gtggccgat 
      atttccttt caaagacaa cacattttg catcgggac 
      agtttatcc gagttgttg gcaacactg gaaattatt 
      aaaacactt aaggaggaa tacagaata agtgttccg 
      gtgcaaaat tacttgaca gggcctcaa aacgtaatc 
      agaattttg ctaaacgtt gagatgtgt cccaatgat 
      tttgaccgg tttttgagg aatttcttg tgtaaatat 
      attgtggat aatcacatg gaccccaac gttatactt 
      ttagaggaa attaaaaac ttgatgaaa tacgatgag 
      agatattgg gtgaatatt attagcctg attctggac 
      tgtattaat tcggttgaa attaaactg caaggtgtt 
      caaactatt ttgaattgt gccaaagta ccttggagt 
      gacacattc attttttgg cccagtttg cccaaatat 
      cgcgaaaag ctgattcag cgttttgat gaagccttg 
      cacattatt gacaataat aatgcagac gtgcaacat 
      ttgtgtttg ttgaccgaa accatagta aaagacaag 
      ttgcgactc caagacgaa acgattagt aatttttac 
      acagtcgca ggatcgctt ttcaagcga ttgaacaaa 
      aactcgaac gaattcgaa cgctctttt aatgacaca 
      actttagat ttgatcaaa aatgtgtac aatttgaga 
      aaacatttc ggaataaga acaaccgct ctcaaatcg 
      cacagcgat aagaacaaa atcattgat gaaatcctc 
      gaaaatgtg cgtttggaa ttcgaaaac gagaaacaa 
      accatcgat gaagttttt gcaacttgt cacaaactt 
      tcggtttgt ttgagttgc gattatcat gacattctt 
      ctacgtttt tgtgaaaaa actggcgat tataatttt 
      attgtgaga acggcggaa aaattccac gatcgcgaa 
      acgtccact agaaatttt tgtttgatg gcaattttg 
      ctgttgaga tacattgga gcgtataaa ctaagttta 
      ggggacagt ttttccgat tctgacagg agtttggtt 
      gttgccgat tcagagcgt gttgttagc gatgatgtt 
      ttttgtaaa ggtttgcgc atagctaca agtattgta 
      gccaaagct atggctaaa gcagaacca gatgagctt 
      atgccgtgt atggaagtt gtaaattgg gtttatgtt 
      gcgttccat ttcactggg agtcaagag agtgatttg 
      agtaaacta ctgtttagg aataattac aggccactt 
      caaatggtt tcaggattt catactttt tcagcgatt 
      aaattggtt tttaacacc ttttgtgga tttgtagcc 
      catgtaaaa agcacgaac agcgcctat ttgacccac 
      ttcaaaaac caacctcta actgaggaa gaatttatg 
      catgaactt caacctcta atccccata ttagaaaac 
      ttgagtaaa gatggccaa cattacacc tcattcgta 
      atcgtctcg actttaaca aattgtttg atgcaatat 
      aacccactt gccaaaaat ctaatcaca accttcgat 
      aaaatattg aagaagaaa tgtattccg aaattattg 
      tctgttgtt gttagtagt aacaccatc gatacagat 
      ctcctaaac aatttatta caattatgc gatcgcgaa 
      gatgcaaca aaattcatc ttaaacgca cttcgaatg 
      tacaaaaga catccaaga aaattcgag tgtctcact 
      cgaaccggc cttaaaata tgcgatcat tacgaatta 
      actgattac aaacgcgta atgatggac tcattgatc 
      accctaaag tggtggaaa agattcgaa gggcacaag 
      ataccctat gataaattt ttcaaaagc aaaccggaa 
      caacgcttc gaattgctg attaacact ttggattgt 
      ttccatatc gagtggctc aacgaattt tgcaaggac 
      tacaaactt gagcctcaa acttgttat ttgttctac 
      ttaaagcga gtgttacaa aattggaaa cccgactat 
      gaaatcaaa agtgatgat acaaatcgg cgattttta 
      attgtaaaa aatagtgaa gttgaattg ttagcaaag 
      tgtcgtgag attatgcaa ggtttaaac aacgaacaa 
      atttctggc gttgttaga acagttcgc aactcgatt 
      aacacctac tactatgaa gttttcatc acgattttg 
      gatatttta tgcgaggtt gacccacaa gagagtcag 
      tttaacgaa ctggcaatg ttacagttc ttgaagaat 
      tatcaaaga gttagtaaa gctagtcaa attgagatt 
      gagcaatgg tttacgaat tttcccgac gctcaaacc 
      gttgatgtc ctctccgaa tttcgacta gctttcact 
      cctctcctc ttcacaagc gatatcttc acaataatt 
      cgccccgaa atcaattta aaaacctac cacttgtgg 
      ttcaataca atcgaaata cttcgccca catttgagc 
      aaaaaccag atttgttca tacgtcgta aaacacgtt 
      gtcgcaagt ggcgttttg tcgaaaagt gacgaaacg 
      tgggttttg aacccgaaa catgacgat ttgttacga 
      gccatagac gagtgtgtt aacaacatc accgatccc 
      gaactggca acgtcggta atgtaccac gtgatgacc 
      aacacttgt cccggattg gaccaagtc aaggccgcc 
      agtctgtgc tacaaatat gccacgaaa tatcgacaa 
      ctaaacccc gattcgagt gcggtcgag aaggctgtg 
      accaaagtc gagaaaaaa tattatatg taccgtgcg 
      ttacacatc ctgaatgtg catcacttg actgattcc 
      aaatacatt gatttgttg acgcacccg aatgatttg 
      atcctagct ttgtatctc gatgatcgc attttggac 
      gattttcat tgtgttgag atcaacaag gcaagcgag 
      gcgttagcc gagctgttt cagttagat attaaaaaa 
      attcgcttg aagttgatt aattcgtgg ctggatgat 
      caggcgttg gttgatttt gactcctcg gtgattgaa 
      tcaaattgc agtgaggag agttgtttg aaacgcatt 
      gcatatatt tgtagcggg gatcaggtg acttactgg 
      caaactgtt cttttggag attggtcag aaaggcgag 
      agtgttcgt tgtagagcc aatgccttg cgtgtgttt 
      gcgatgatt tcggatgtt gaaactatt gagacaatg 
      gccgaaatg agttatgac agactgatg ttgtttatt 
      aataggctg acgcttgtt ggtaacttg gaatatttg 
      ggatttggt gtagatgtt gccgttctg gaccacaca 
      aataggcaa tccttgttg aatcggttg gtgcaacaa 
      ggaggcaat gcattcgct attaaaaca atggctttg 
      tactgtatg acgtaccag atttatggt gcgaaatat 
      tgggagttt gttttaaac aacgctgtc aaattttct 
      ttgtttacc gaattgaaa acctatgtt acgttcctc 
      agcaaaagt aaataccag tacaaggga ttttacata 
      aatgcttgg caaaaattg attgattat tatttctcc 
      aacttgaat tgtgccaaa aatttggac caaacactc 
      ctcaaacat ttcttaaca attcagtcg tgtcccgtt 
      ttgttcagt cttgatttt aatcatgca tttgcaatg 
      tgtttggag gtaaagaaa gcagaattt gccgcagtt 
      ttattacaa taccttcaa aaaaatgac acctatttt 
      tcgaatgtc gagaaagta cgtaattgt agtaaggaa 
      ataatgcca ggtttgaat tatttggac cagaatgga 
      atcttgggg atatcgaat gtacgggag attttgcat 
      aatagtgtt taa
    mRNA sequence
    • >TC034281 RA
      ccaattttt gagttcaat ttaacgaac tttactaaa 
      taaaaaaca atacttttt tacaagtga ttgtcaaga 
      caaaagtgt tcgaataaa tcgttataa ccccatttc 
      tagtgttgt tgttatttg aggttaagt cacgcaatt 
      cgggggcaa aaccataaa aaatgtcta attttgata 
      taattgagt tcggcttta acacagctg atgaaaccg 
      ttaacttcg gaactcgaa gtttagcag acggagaca 
      atttatacg aagcctcaa ctttagcca cccttaaac 
      catcaaatg gggctgacg cgaatttgg acccttata 
      tcaacgccg ttgtaaaaa atggctcaa tttatttca 
      gtattgata attcgctgc gtatttttg aagacaaac 
      agtgctctt atgtcgctt ttagtgcaa cttttagcg 
      ctgttgtgg actcgtttt gtgtctcgt ccgattgcg 
      aatatttat tagtttgtc tcaggaatg gcacagtgc 
      aattgattg accaaaaca acgaaaata aatgtgttt 
      tcaccgatt tatgtgaac gggatgagg attgtgatc 
      gcttcttta tcaattctt ttgtttaca atttatgct 
      ttgtttttg tcacaaaac agggaaaaa tttaccaac 
      tggacccca gacactttg aactgaaat gtgttcatg 
      tgtttaatt ttgcaacaa atgtggcga gttttaaag 
      ccccttttt tgctactta gtggtacaa aattggcgg 
      ttttaaatt tggaaagtc gtaaaagtg tcattgatt 
      ccgaggaca atcagttta agaaaattt acccattaa 
      gaaataatt tttttgcct tggattttg agggaaaat 
      tatacaaga tttgtgcaa tgactcttt tgatttttc 
      cgattaatt cgcaacact tgtatagag acatgcttc 
      taatttctg attcgaatg aagaacagg ttgttgtga 
      cggaagatg aaatgcagc aaaaaagtc ttcagttac 
      tttcatatc cagccttga gtaaaatat acggtttga 
      acgtgaaaa ccgacatct atttaatat ctccagaat 
      gtgtaaacg atgagcttt tatacattt ctaaaattg 
      tcgagaaca attcagtgt cggaattgc gttttcaca 
      tggtttatg aaactgatg ccgaacaga gacttgcaa 
      gattgttaa gaaaacgca aatatgaag aagctgaac 
      gttttgctg ctcttttcc acttggatg cgagtcttg 
      tgaacgagg caaaagccc aagatattg tcgataaaa 
      tgacttgca ctagtgaag aaatcgacc aacttatca 
      atcttttga atttaatcg acaacgatg tgttcaaac 
      tgcagtctt gtttgggtg taacttgta gtaaatttg 
      aagatgttc gcaaaatac tcgagtatg gttgtacat 
      tgcaattga agaaagtgg acaacgaag ctgaaaaaa 
      tgcaaaaca gtgtaattg atgttatgg caaggtttg 
      agacgtttg gggcgcttt caaaaaggg ctgagttgg 
      atatacaga cttggcaac aattcgcaa acgctgatt 
      tgatggatg aggtcaaga gtttcctgc aaaatcatg 
      aaatcgaag aagccaaaa taatttaca gtcgcttag 
      acgtcgcca ctatcgaaa ccctaactg acgacaaaa 
      tttcagaca ttttaaaca tcttaaata tgctaccac 
      cagctacct atcagtctt tcctatcaa ttttcattc 
      cagtaacac tttcacttt accctacag cccttcctc 
      taatcgtta aatggacac gcaataaag tgttttctt 
      tggagcaac attcaaaga gcgattttc caagcaatg 
      caattattt taacgaaaa aaataatcg cattgatga 
      agatgggcg atgtggccg atatttcct ttcaaagac 
      aacacattt tgcatcggg acagtttat ccgagttgt 
      tggcaacac tggaaatta ttaaaacac ttaaggagg 
      aatacagaa taagtgttc cggtgcaaa attacttga 
      cagggcctc aaaacgtaa tcagaattt tgctaaacg 
      ttgagatgt gtcccaatg attttgacc ggtttttga 
      ggaatttct tgtgtaaat atattgtgg ataatcaca 
      tggacccca acgttatac ttttagagg aaattaaaa 
      acttgatga aatacgatg agagatatt gggtgaata 
      ttattagcc tgattctgg actgtatta attcggttg 
      aaattaaac tgcaaggtg ttcaaacta ttttgaatt 
      gtgccaaag taccttgga gtgacacat tcatttttt 
      ggcccagtt tgcccaaat atcgcgaaa agctgattc 
      agcgttttg atgaagcct tgcacatta ttgacaata 
      ataatgcag acgtgcaac atttgtgtt tgttgaccg 
      aaaccatag taaaagaca agttgcgac tccaagacg 
      aaacgatta gtaattttt acacagtcg caggatcgc 
      ttttcaagc gattgaaca aaaactcga acgaattcg 
      aacgctctt ttaatgaca caactttag atttgatca 
      aaaatgtgt acaatttga gaaaacatt tcggaataa 
      gaacaaccg ctctcaaat cgcacagcg ataagaaca 
      aaatcattg atgaaatcc tcgaaaatg tgcgtttgg 
      aattcgaaa acgagaaac aaaccatcg atgaagttt 
      ttgcaactt gtcacaaac tttcggttt gtttgagtt 
      gcgattatc atgacattc ttctacgtt tttgtgaaa 
      aaactggcg attataatt ttattgtga gaacggcgg 
      aaaaattcc acgatcgcg aaacgtcca ctagaaatt 
      tttgtttga tggcaattt tgctgttga gatacattg 
      gagcgtata aactaagtt taggggaca gtttttccg 
      attctgaca ggagtttgg ttgttgccg attcagagc 
      gtgttgtta gcgatgatg ttttttgta aaggtttgc 
      gcatagcta caagtattg tagccaaag ctatggcta 
      aagcagaac cagatgagc ttatgccgt gtatggaag 
      ttgtaaatt gggtttatg ttgcgttcc atttcactg 
      ggagtcaag agagtgatt tgagtaaac tactgttta 
      ggaataatt acaggccac ttcaaatgg tttcaggat 
      ttcatactt tttcagcga ttaaattgg tttttaaca 
      ccttttgtg gatttgtag cccatgtaa aaagcacga 
      acagcgcct atttgaccc acttcaaaa accaacctc 
      taactgagg aagaattta tgcatgaac ttcaacctc 
      taatcccca tattagaaa acttgagta aagatggcc 
      aacattaca cctcattcg taatcgtct cgactttaa 
      caaattgtt tgatgcaat ataacccac ttgccaaaa 
      atctaatca caaccttcg ataaaatat tgaagaaga 
      aatgtattc cgaaattat tgtctgttg ttgttagta 
      gtaacacca tcgatacag atctcctaa acaatttat 
      tacaattat gcgatcgcg aagatgcaa caaaattca 
      tcttaaacg cacttcgaa tgtacaaaa gacatccaa 
      gaaaattcg agtgtctca ctcgaaccg gccttaaaa 
      tatgcgatc attacgaat taactgatt acaaacgcg 
      taatgatgg actcattga tcaccctaa agtggtgga 
      aaagattcg aagggcaca agataccct atgataaat 
      ttttcaaaa gcaaaccgg aacaacgct tcgaattgc 
      tgattaaca ctttggatt gtttccata tcgagtggc 
      tcaacgaat tttgcaagg actacaaac ttgagcctc 
      aaacttgtt atttgttct acttaaagc gagtgttac 
      aaaattgga aacccgact atgaaatca aaagtgatg 
      atacaaatc ggcgatttt taattgtaa aaaatagtg 
      aagttgaat tgttagcaa agtgtcgtg agattatgc 
      aaggtttaa acaacgaac aaatttctg gcgttgtta 
      gaacagttc gcaactcga ttaacacct actactatg 
      aagttttca tcacgattt tggatattt tatgcgagg 
      ttgacccac aagagagtc agtttaacg aactggcaa 
      tgttacagt tcttgaaga attatcaaa gagttagta 
      aagctagtc aaattgaga ttgagcaat ggtttacga 
      attttcccg acgctcaaa ccgttgatg tcctctccg 
      aatttcgac tagctttca ctcctctcc tcttcacaa 
      gcgatatct tcacaataa ttcgccccg aaatcaatt 
      taaaaacct accacttgt ggttcaata caatcgaaa 
      tacttcgcc cacatttga gcaaaaacc agatttgtt 
      catacgtcg taaaacacg ttgtcgcaa gtggcgttt 
      tgtcgaaaa gtgacgaaa cgtgggttt tgaacccga 
      aacatgacg atttgttac gagccatag acgagtgtg 
      ttaacaaca tcaccgatc ccgaactgg caacgtcgg 
      taatgtacc acgtgatga ccaacactt gtcccggat 
      tggaccaag tcaaggccg ccagtctgt gctacaaat 
      atgccacga aatatcgac aactaaacc ccgattcga 
      gtgcggtcg agaaggctg tgaccaaag tcgagaaaa 
      aatattata tgtaccgtg cgttacaca tcctgaatg 
      tgcatcact tgactgatt ccaaataca ttgatttgt 
      tgacgcacc cgaatgatt tgatcctag ctttgtatc 
      tcgatgatc gcattttgg acgattttc attgtgttg 
      agatcaaca aggcaagcg aggcgttag ccgagctgt 
      ttcagttag atattaaaa aaattcgct tgaagttga 
      ttaattcgt ggctggatg atcaggcgt tggttgatt 
      ttgactcct cggtgattg aatcaaatt gcagtgagg 
      agagttgtt tgaaacgca ttgcatata tttgtagcg 
      gggatcagg tgacttact ggcaaactg ttcttttgg 
      agattggtc agaaaggcg agagtgttc gttgtagag 
      ccaatgcct tgcgtgtgt ttgcgatga tttcggatg 
      ttgaaacta ttgagacaa tggccgaaa tgagttatg 
      acagactga tgttgttta ttaataggc tgacgcttg 
      ttggtaact tggaatatt tgggatttg gtgtagatg 
      ttgccgttc tggaccaca caaataggc aatccttgt 
      tgaatcggt tggtgcaac aaggaggca atgcattcg 
      ctattaaaa caatggctt tgtactgta tgacgtacc 
      agatttatg gtgcgaaat attgggagt ttgttttaa 
      acaacgctg tcaaatttt ctttgttta ccgaattga 
      aaacctatg ttacgttcc tcagcaaaa gtaaatacc 
      agtacaagg gattttaca taaatgctt ggcaaaaat 
      tgattgatt attatttct ccaacttga attgtgcca 
      aaaatttgg accaaacac tcctcaaac atttcttaa 
      caattcagt cgtgtcccg ttttgttca gtcttgatt 
      ttaatcatg catttgcaa tgtgtttgg aggtaaaga 
      aagcagaat ttgccgcag ttttattac aataccttc 
      aaaaaaatg acacctatt tttcgaatg tcgagaaag 
      tacgtaatt gtagtaagg aaataatgc caggtttga 
      attatttgg accagaatg gaatcttgg ggatatcga 
      atgtacggg agattttgc ataatagtg tttaataaa 
      ttttctgtt ttttttttg ttttttcat taataaaag 
      aattctcaa aat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY