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Gene information
General information
TC005178 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 4415210..4420510
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005178
    iBeetle screen iB_00813
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (22 dpi)
    Days post injection


    Adult morphology
    motility decreased - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005178 RA
      MSGYETNLY LYSEEMEDR PRISTLISS LANYSNTIP 
      PASNDPDAR PAGGGARMG TLMGVYLPC IQNIFGVIL 
      FIRLTWVVG TAGAIQGFL IVLCCCCVT MLTAISMSA 
      IATNGVVPG GGSYFMISR SLGPEFGGA VGMLFYTGT 
      TLAAAMYIV GAVEIVLTY MAPWLSIFG DFTTNQDAM 
      YNNFRVYGT GLLVVMGTI VFVGVKFVN KFAAVALAC 
      VILSILAVY IGIFRNING KDDLQMCVL GKRLLRVPA 
      ADCYKNGSG FIHNMFCPN GTASCDPYY ERNDVRLVQ 
      GIKGLSSGV ILDNIYDSF LENGQYIAI GNNPEDIEP 
      MEPHRDTAQ VTADITTTF TILIGIFFP SVTGIMAGS 
      NRSGDLADA QKSIPIGTI CAILTTSTV YLSSVILFA 
      ATVDNLLLR DKFGSSIGG KLVVANMAW PNEWVILIG 
      SFLSTLGAG LQSLTGAPR LLQAIAKDG IIPFLSPFA 
      VSSSRGEPT RALLLTIVI CQCGILLGN VDLLAPLLS 
      MFFLMCYGF VNLACALQT LLRTPNWRP RFKYYHWSL 
      SFIGLSLCI AIMFMTSWY FALLAMGMA GLIYKYIEY 
      RGAEKEWGD GIRGLALSA ARYSLLRLE EGPPHTKNW 
      RPQLLILVK LTTDLVPKY RKLFAFASQ LKAGKGLTV 
      CAAVIGGDF TRSYGEAMA AKQSLRKTM DEERVKGFV 
      DILVARNIT EGLSNLVQT SGLGGMKPN TVILGWPYG 
      WRQSEDERT WQVFLQTVR NVTAAHMAL LVPKGINFF 
      PDSTEKVFG NIDIWWIVH DGGLLMLLP FLLKQHRTW 
      KNCKMRIFT VAQMEDNSI QMKKDLKTF LYHLRIEAE 
      VEVVEMMDS DISAYTYER TLMMEQRNQ MLRELRLNK 
      KESLGVVDL VDFNEMSTE EKLPLVQSI VDQHHQNID 
      AKTATKVRF QEPSAEGDK DNKAAETEP TNEPNSQTK 
      PEDNDNSTT EKEDPSEKE CNEETKENN ISSEEKKKP 
      ATITPDEGN VRRMHTAVK LNEVIVNRS HEAQLVILN 
      LPGPPKDTK MERESNYME FLEVLTEGL ERVLMVRGG 
      GQEVITIYS
    • >TC005178 RB
      MSGYETNLY LYSEEMEDR PRISTLISS LANYSNTIP 
      PASNDPDAR PAGGGARMG TLMGVYLPC IQNIFGVIL 
      FIRLTWVVG TAGAIQGFL IVLCCCCVT MLTAISMSA 
      IATNGVVPG GGSYFMISR SLGPEFGGA VGMLFYTGT 
      TLAAAMYIV GAVEIVLTY MAPWLSIFG DFTTNQDAM 
      YNNFRVYGT GLLVVMGTI VFVGVKFVN KFAAVALAC 
      VILSILAVY IGIFRNING KDDLQMCVL GKRLLRVPA 
      ADCYKNGSG FIHNMFCPN GTASCDPYY ERNDVRLVQ 
      GIKGLSSGV ILDNIYDSF LENGQYIAI GNNPEDIEP 
      MEPHRDTAQ VTADITTTF TILIGIFFP SVTGIMAGS 
      NRSGDLADA QKSIPIGTI CAILTTSTV YLSSVILFA 
      ATVDNLLLR DKFGSSIGG KLVVANMAW PNEWVILIG 
      SFLSTLGAG LQSLTGAPR LLQAIAKDG IIPFLSPFA 
      VSSSRGEPT RALLLTIVI CQCGILLGN VDLLAPLLS 
      MFFLMCYGF VNLACALQT LLRTPNWRP RFKYYHWSL 
      SFIGLSLCI AIMFMTSWY FALLAMGMA GLIYKYIEY 
      RGAEKEWGD GIRGLALSA ARYSLLRLE EGPPHTKNW 
      RPQLLILVK LTTDLVPKY RKLFAFASQ LKAGKGLTV 
      CAAVIGGDF TRSYGEAMA AKQSLRKTM DEERVKGFV 
      DILVARNIT EGLSNLVQT SGLGGMKPN TVILGWPYG 
      WRQSEDERT WQVFLQTVR NVTAAHMAL LVPKGINFF 
      PDSTEKVFG NIDIWWIVH DGGLLMLLP FLLKQHRTW 
      KNCKMRIFT VAQMEDNSI QMKKDLKTF LYHLRIEAE 
      VEVVEMMDS DISAYTYER TLMMEQRNQ MLRELRLNK 
      KESLGVVQS IVDQHHQNI DAKTATKVR FQEPSAEGD 
      KDNKAAETE PTNEPNSQT KPEDNDNST TEKEDPSEK 
      ECNEETKEN NISSEEKKK PATITPDEG NVRRMHTAV 
      KLNEVIVNR SHEAQLVIL NLPGPPKDT KMERESNYM 
      EFLEVLTEG LERVLMVRG GGQEVITIY S
    CDS sequence
    • >TC005178 RA
      atgtcgggg tacgagacg aacttgtac ctctactcc 
      gaggagatg gaggacaga cccaggata tctaccctc 
      ataagctca ttagcgaac tactcgaac accattcct 
      ccagcatcc aatgatccc gatgcacgg ccggccggt 
      gggggcgcc cgtatgggc accctcatg ggcgtctat 
      ctcccctgt atccagaac atcttcggc gtgattctt 
      ttcattcgg ttgacgtgg gtggtgggc acagcaggg 
      gcgattcaa ggcttcctt atcgtgttg tgctgttgc 
      tgcgtcacc atgctgacc gccatctcc atgtcggcg 
      atcgccacc aacggggtg gtgcccggg ggcggcagt 
      tatttcatg atatccagg tcacttggt ccagagttt 
      ggcggcgct gttggcatg ctgttttac acagggacg 
      acgttagca gccgcgatg tacatcgtc ggtgctgtt 
      gaaattgtc ttgacctac atggctcct tggttatca 
      atttttggg gattttaca acaaatcag gacgcaatg 
      tacaacaat tttcgggtc tatggaact gggttactc 
      gtggtgatg ggaactatt gtgttcgtc ggggtgaag 
      tttgtcaac aaatttgcc gccgttgct cttgcatgc 
      gtcattctc tccatactg gctgtgtac attggaata 
      ttccgcaat atcaacggc aaagatgac ttgcaaatg 
      tgcgttctt ggtaaacga ttgttacgt gtcccggct 
      gctgattgt tacaaaaac ggtagtggt ttcatacac 
      aacatgttt tgtcctaat ggaaccgct agttgcgat 
      ccgtactat gaacgtaat gacgtaagg ctagttcaa 
      gggattaaa gggttgtca tccggtgtg attttggac 
      aatatctac gatagcttc ttggaaaat ggacagtac 
      atagcaata gggaataat cctgaggat attgagcca 
      atggaacct cacagagac actgcccaa gtgacggca 
      gatataaca acaactttt acaattttg atcggaata 
      tttttccct tcagtgact ggtattatg gctgggtct 
      aatcgttcg ggtgattta gctgatgcc caaaaatct 
      ataccaatc ggaacaatt tgtgccatt ttaacaacg 
      tcgactgtg tatttatca tctgtgatt ctcttcgct 
      gctactgtc gacaacctt ctacttaga gataaattt 
      ggctcatct ataggaggt aaattagtt gtagccaac 
      atggcgtgg ccaaacgaa tgggtcatt ttaatcgga 
      tcattcctt tcgaccctt ggcgctggt ctccagtca 
      ctgactgga gctcctcgc ttactccaa gccatcgcc 
      aaagacggt atcattcca tttttatca ccgtttgcc 
      gtatcatca agccgtggc gagccaacc cgagcatta 
      ttattaact attgtcatt tgtcagtgt ggtatatta 
      ttaggaaat gttgatctt ttggcgccc ttactctcg 
      atgtttttc ttaatgtgc tacggtttt gtaaatctc 
      gcttgcgct ttacaaaca ttactacga acacccaac 
      tggagacca agatttaaa tattaccat tggtccctc 
      tcatttatc ggcttatcg ttgtgcata gcaataatg 
      tttatgacc tcttggtac tttgcgctt ctcgccatg 
      ggaatggca ggcttgatc tataaatac atcgaatac 
      agaggtgcc gagaaagaa tggggtgac ggcattcga 
      ggtctagcg ttgtcagca gcgcgatat tcattactc 
      cgcctagaa gagggtcct ccccacacc aaaaactgg 
      agacctcag ctcctcatt ctcgttaaa ttaacaact 
      gatctcgtt ccgaaatac agaaaatta tttgctttc 
      gcgtcgcaa ctcaaagct gggaagggt ttgacagtt 
      tgcgctgct gtaattggc ggcgatttt acacgatcg 
      tacggagaa gcgatggca gctaaacaa agcttgaga 
      aaaaccatg gacgaagaa cgggtcaag ggttttgtc 
      gatattcta gtggcacgc aacattacc gaaggatta 
      tctaactta gtgcaaact tctggtctt ggtggcatg 
      aagccgaat acggtgatt ttaggatgg ccgtatggc 
      tggcgacag tcggaagat gagcgcacg tggcaggtt 
      ttcttgcaa actgttcgg aatgtgaca gccgcacac 
      atggcgtta ttagttcct aaaggtatc aactttttc 
      ccggactct acggagaaa gtcttcggg aatatcgat 
      atctggtgg attgttcat gacggtggt cttctcatg 
      ttgttaccc ttccttctc aaacagcac cgcacttgg 
      aaaaattgc aagatgcgc attttcact gttgctcaa 
      atggaagac aattccatc caaatgaag aaagatcta 
      aagacgttc ttgtatcac ctccgaatt gaagcagaa 
      gttgaagtc gttgaaatg atggacagc gatatttcg 
      gcatacacc tacgaaagg actctgatg atggaacag 
      cggaatcaa atgttgcga gaacttcgt ctcaacaaa 
      aaagaaagt ttgggtgtg gtggatttg gtggatttc 
      aatgaaatg tcaactgaa gagaagctg cctctggtt 
      cagtcaatt gtagatcaa caccatcaa aatatcgat 
      gctaaaaca gccaccaaa gttaggttc caggaacct 
      agcgctgaa ggcgataaa gataacaaa gcggctgaa 
      acggaaccg acaaacgag cctaattca cagacgaaa 
      cccgaggat aacgataat tcaacaaca gagaaagaa 
      gatccgtcg gagaaagaa tgcaatgag gaaacaaaa 
      gagaacaat atctcctcc gaagagaaa aagaaaccc 
      gcaacgata acccctgac gaaggcaac gtccgtcgg 
      atgcataca gctgtaaaa ttaaacgaa gtgatagtg 
      aatcgttct cacgaagct cagcttgtg atcttgaat 
      ttacccggc cctccaaaa gacaccaag atggaaaga 
      gaatccaat tatatggaa ttcttggaa gtgctcact 
      gaaggtttg gaacgtgtg ctcatggtc cgtggtggc 
      ggtcaagag gtcataact atctattcg taa
    • >TC005178 RB
      atgtcgggg tacgagacg aacttgtac ctctactcc 
      gaggagatg gaggacaga cccaggata tctaccctc 
      ataagctca ttagcgaac tactcgaac accattcct 
      ccagcatcc aatgatccc gatgcacgg ccggccggt 
      gggggcgcc cgtatgggc accctcatg ggcgtctat 
      ctcccctgt atccagaac atcttcggc gtgattctt 
      ttcattcgg ttgacgtgg gtggtgggc acagcaggg 
      gcgattcaa ggcttcctt atcgtgttg tgctgttgc 
      tgcgtcacc atgctgacc gccatctcc atgtcggcg 
      atcgccacc aacggggtg gtgcccggg ggcggcagt 
      tatttcatg atatccagg tcacttggt ccagagttt 
      ggcggcgct gttggcatg ctgttttac acagggacg 
      acgttagca gccgcgatg tacatcgtc ggtgctgtt 
      gaaattgtc ttgacctac atggctcct tggttatca 
      atttttggg gattttaca acaaatcag gacgcaatg 
      tacaacaat tttcgggtc tatggaact gggttactc 
      gtggtgatg ggaactatt gtgttcgtc ggggtgaag 
      tttgtcaac aaatttgcc gccgttgct cttgcatgc 
      gtcattctc tccatactg gctgtgtac attggaata 
      ttccgcaat atcaacggc aaagatgac ttgcaaatg 
      tgcgttctt ggtaaacga ttgttacgt gtcccggct 
      gctgattgt tacaaaaac ggtagtggt ttcatacac 
      aacatgttt tgtcctaat ggaaccgct agttgcgat 
      ccgtactat gaacgtaat gacgtaagg ctagttcaa 
      gggattaaa gggttgtca tccggtgtg attttggac 
      aatatctac gatagcttc ttggaaaat ggacagtac 
      atagcaata gggaataat cctgaggat attgagcca 
      atggaacct cacagagac actgcccaa gtgacggca 
      gatataaca acaactttt acaattttg atcggaata 
      tttttccct tcagtgact ggtattatg gctgggtct 
      aatcgttcg ggtgattta gctgatgcc caaaaatct 
      ataccaatc ggaacaatt tgtgccatt ttaacaacg 
      tcgactgtg tatttatca tctgtgatt ctcttcgct 
      gctactgtc gacaacctt ctacttaga gataaattt 
      ggctcatct ataggaggt aaattagtt gtagccaac 
      atggcgtgg ccaaacgaa tgggtcatt ttaatcgga 
      tcattcctt tcgaccctt ggcgctggt ctccagtca 
      ctgactgga gctcctcgc ttactccaa gccatcgcc 
      aaagacggt atcattcca tttttatca ccgtttgcc 
      gtatcatca agccgtggc gagccaacc cgagcatta 
      ttattaact attgtcatt tgtcagtgt ggtatatta 
      ttaggaaat gttgatctt ttggcgccc ttactctcg 
      atgtttttc ttaatgtgc tacggtttt gtaaatctc 
      gcttgcgct ttacaaaca ttactacga acacccaac 
      tggagacca agatttaaa tattaccat tggtccctc 
      tcatttatc ggcttatcg ttgtgcata gcaataatg 
      tttatgacc tcttggtac tttgcgctt ctcgccatg 
      ggaatggca ggcttgatc tataaatac atcgaatac 
      agaggtgcc gagaaagaa tggggtgac ggcattcga 
      ggtctagcg ttgtcagca gcgcgatat tcattactc 
      cgcctagaa gagggtcct ccccacacc aaaaactgg 
      agacctcag ctcctcatt ctcgttaaa ttaacaact 
      gatctcgtt ccgaaatac agaaaatta tttgctttc 
      gcgtcgcaa ctcaaagct gggaagggt ttgacagtt 
      tgcgctgct gtaattggc ggcgatttt acacgatcg 
      tacggagaa gcgatggca gctaaacaa agcttgaga 
      aaaaccatg gacgaagaa cgggtcaag ggttttgtc 
      gatattcta gtggcacgc aacattacc gaaggatta 
      tctaactta gtgcaaact tctggtctt ggtggcatg 
      aagccgaat acggtgatt ttaggatgg ccgtatggc 
      tggcgacag tcggaagat gagcgcacg tggcaggtt 
      ttcttgcaa actgttcgg aatgtgaca gccgcacac 
      atggcgtta ttagttcct aaaggtatc aactttttc 
      ccggactct acggagaaa gtcttcggg aatatcgat 
      atctggtgg attgttcat gacggtggt cttctcatg 
      ttgttaccc ttccttctc aaacagcac cgcacttgg 
      aaaaattgc aagatgcgc attttcact gttgctcaa 
      atggaagac aattccatc caaatgaag aaagatcta 
      aagacgttc ttgtatcac ctccgaatt gaagcagaa 
      gttgaagtc gttgaaatg atggacagc gatatttcg 
      gcatacacc tacgaaagg actctgatg atggaacag 
      cggaatcaa atgttgcga gaacttcgt ctcaacaaa 
      aaagaaagt ttgggtgtg gttcagtca attgtagat 
      caacaccat caaaatatc gatgctaaa acagccacc 
      aaagttagg ttccaggaa cctagcgct gaaggcgat 
      aaagataac aaagcggct gaaacggaa ccgacaaac 
      gagcctaat tcacagacg aaacccgag gataacgat 
      aattcaaca acagagaaa gaagatccg tcggagaaa 
      gaatgcaat gaggaaaca aaagagaac aatatctcc 
      tccgaagag aaaaagaaa cccgcaacg ataacccct 
      gacgaaggc aacgtccgt cggatgcat acagctgta 
      aaattaaac gaagtgata gtgaatcgt tctcacgaa 
      gctcagctt gtgatcttg aatttaccc ggccctcca 
      aaagacacc aagatggaa agagaatcc aattatatg 
      gaattcttg gaagtgctc actgaaggt ttggaacgt 
      gtgctcatg gtccgtggt ggcggtcaa gaggtcata 
      actatctat tcgtaa
    mRNA sequence
    • >TC005178 RA
      ggttgcagg agatggcgc cgaagagca accttttgt 
      gtcggaaaa gatgtcggg gtacgagac gaacttgta 
      cctctactc cgaggagat ggaggacag acccaggat 
      atctaccct cataagctc attagcgaa ctactcgaa 
      caccattcc tccagcatc caatgatcc cgatgcacg 
      gccggccgg tgggggcgc ccgtatggg caccctcat 
      gggcgtcta tctcccctg tatccagaa catcttcgg 
      cgtgattct tttcattcg gttgacgtg ggtggtggg 
      cacagcagg ggcgattca aggcttcct tatcgtgtt 
      gtgctgttg ctgcgtcac catgctgac cgccatctc 
      catgtcggc gatcgccac caacggggt ggtgcccgg 
      gggcggcag ttatttcat gatatccag gtcacttgg 
      tccagagtt tggcggcgc tgttggcat gctgtttta 
      cacagggac gacgttagc agccgcgat gtacatcgt 
      cggtgctgt tgaaattgt cttgaccta catggctcc 
      ttggttatc aatttttgg ggattttac aacaaatca 
      ggacgcaat gtacaacaa ttttcgggt ctatggaac 
      tgggttact cgtggtgat gggaactat tgtgttcgt 
      cggggtgaa gtttgtcaa caaatttgc cgccgttgc 
      tcttgcatg cgtcattct ctccatact ggctgtgta 
      cattggaat attccgcaa tatcaacgg caaagatga 
      cttgcaaat gtgcgttct tggtaaacg attgttacg 
      tgtcccggc tgctgattg ttacaaaaa cggtagtgg 
      tttcataca caacatgtt ttgtcctaa tggaaccgc 
      tagttgcga tccgtacta tgaacgtaa tgacgtaag 
      gctagttca agggattaa agggttgtc atccggtgt 
      gattttgga caatatcta cgatagctt cttggaaaa 
      tggacagta catagcaat agggaataa tcctgagga 
      tattgagcc aatggaacc tcacagaga cactgccca 
      agtgacggc agatataac aacaacttt tacaatttt 
      gatcggaat atttttccc ttcagtgac tggtattat 
      ggctgggtc taatcgttc gggtgattt agctgatgc 
      ccaaaaatc tataccaat cggaacaat ttgtgccat 
      tttaacaac gtcgactgt gtatttatc atctgtgat 
      tctcttcgc tgctactgt cgacaacct tctacttag 
      agataaatt tggctcatc tataggagg taaattagt 
      tgtagccaa catggcgtg gccaaacga atgggtcat 
      tttaatcgg atcattcct ttcgaccct tggcgctgg 
      tctccagtc actgactgg agctcctcg cttactcca 
      agccatcgc caaagacgg tatcattcc atttttatc 
      accgtttgc cgtatcatc aagccgtgg cgagccaac 
      ccgagcatt attattaac tattgtcat ttgtcagtg 
      tggtatatt attaggaaa tgttgatct tttggcgcc 
      cttactctc gatgttttt cttaatgtg ctacggttt 
      tgtaaatct cgcttgcgc tttacaaac attactacg 
      aacacccaa ctggagacc aagatttaa atattacca 
      ttggtccct ctcatttat cggcttatc gttgtgcat 
      agcaataat gtttatgac ctcttggta ctttgcgct 
      tctcgccat gggaatggc aggcttgat ctataaata 
      catcgaata cagaggtgc cgagaaaga atggggtga 
      cggcattcg aggtctagc gttgtcagc agcgcgata 
      ttcattact ccgcctaga agagggtcc tccccacac 
      caaaaactg gagacctca gctcctcat tctcgttaa 
      attaacaac tgatctcgt tccgaaata cagaaaatt 
      atttgcttt cgcgtcgca actcaaagc tgggaaggg 
      tttgacagt ttgcgctgc tgtaattgg cggcgattt 
      tacacgatc gtacggaga agcgatggc agctaaaca 
      aagcttgag aaaaaccat ggacgaaga acgggtcaa 
      gggttttgt cgatattct agtggcacg caacattac 
      cgaaggatt atctaactt agtgcaaac ttctggtct 
      tggtggcat gaagccgaa tacggtgat tttaggatg 
      gccgtatgg ctggcgaca gtcggaaga tgagcgcac 
      gtggcaggt tttcttgca aactgttcg gaatgtgac 
      agccgcaca catggcgtt attagttcc taaaggtat 
      caacttttt cccggactc tacggagaa agtcttcgg 
      gaatatcga tatctggtg gattgttca tgacggtgg 
      tcttctcat gttgttacc cttccttct caaacagca 
      ccgcacttg gaaaaattg caagatgcg cattttcac 
      tgttgctca aatggaaga caattccat ccaaatgaa 
      gaaagatct aaagacgtt cttgtatca cctccgaat 
      tgaagcaga agttgaagt cgttgaaat gatggacag 
      cgatatttc ggcatacac ctacgaaag gactctgat 
      gatggaaca gcggaatca aatgttgcg agaacttcg 
      tctcaacaa aaaagaaag tttgggtgt ggtggattt 
      ggtggattt caatgaaat gtcaactga agagaagct 
      gcctctggt tcagtcaat tgtagatca acaccatca 
      aaatatcga tgctaaaac agccaccaa agttaggtt 
      ccaggaacc tagcgctga aggcgataa agataacaa 
      agcggctga aacggaacc gacaaacga gcctaattc 
      acagacgaa acccgagga taacgataa ttcaacaac 
      agagaaaga agatccgtc ggagaaaga atgcaatga 
      ggaaacaaa agagaacaa tatctcctc cgaagagaa 
      aaagaaacc cgcaacgat aacccctga cgaaggcaa 
      cgtccgtcg gatgcatac agctgtaaa attaaacga 
      agtgatagt gaatcgttc tcacgaagc tcagcttgt 
      gatcttgaa tttacccgg ccctccaaa agacaccaa 
      gatggaaag agaatccaa ttatatgga attcttgga 
      agtgctcac tgaaggttt ggaacgtgt gctcatggt 
      ccgtggtgg cggtcaaga ggtcataac tatctattc 
      gtaacttaa atatgggat tagatttcg aaattggag 
      tgtcagttt tgtattgtc tgacagcca agcagttaa 
      tcaaaggac attgctatg gaaatggcg atagccgaa 
      cagtattta attataaaa tgttaccac gtctggata 
      gtgattatg ccaaagaga tacttattt attcttctc 
      aatgttata gattgtatg agcggtgaa gttggagga 
      tgtacaggt agaatgatg gtaatcgtt tttctgtac 
      cgtctcctg ctggaaccg acgtgattc tctatccta 
      tgtaatact gtatcaaat caaaatgat gttccgcga 
      agtgctcac ttttctatg agaacggct gggccatgc 
      caaatgcca cttgagtgc atgcagtgt tatgatagg 
      gcgtagcgt aagacttgg tagaatagc ctaatactg 
      ctaacacta acgagtgta ttcatgtag tttcgttag 
      ttctgttat atatcatat tacatttaa tgtccagcg 
      aatttcttt tacactcaa ctaatatcg atacttaga 
      gtgtcattt tataaattc agtaaacat aaagtgagt 
      gtgtactta agtacgcta tcatctcag tctcattag 
      gtggtaaaa tacgcgtat atggtatat actataaga 
      acattactg agcaatatt aactaataa ttactgtat 
      taaatgtca aattaatta tgaagtgat aggatattc 
      acatgtaca atctatacc gaccgccgg agggcgggc 
      tctccgtgc tgtgacagg tgacagtgc tcactcacg 
      atcagttct cttgcatgc tgcgaccga ctctgtctc 
      gtgcggcct gccgtagag ataatagtg cactagtga 
      tctcaacta gccttaaag gtaccttga aatattaaa 
      ttctattta tttatatct atgtgtcac taaattagc 
      tgctgccag attacgcca ttctacatc aaccactta 
      tgactgttg gtgcccgcg tcccagtgg cgttcaaat 
      agaatttat ttattgtca tccgagcta gtcgttatc 
      acttttata cgttcgatt attatcatt tctacccaa 
      tctccatct atttaccat tattgtaaa tggcgcaat 
      ctgtttatc ttattattg acaatgtgt tcattggat 
      aaaaatatt aaataaaat tacatagta tag
    • >TC005178 RB
      ggttgcagg agatggcgc cgaagagca accttttgt 
      gtcggaaaa gatgtcggg gtacgagac gaacttgta 
      cctctactc cgaggagat ggaggacag acccaggat 
      atctaccct cataagctc attagcgaa ctactcgaa 
      caccattcc tccagcatc caatgatcc cgatgcacg 
      gccggccgg tgggggcgc ccgtatggg caccctcat 
      gggcgtcta tctcccctg tatccagaa catcttcgg 
      cgtgattct tttcattcg gttgacgtg ggtggtggg 
      cacagcagg ggcgattca aggcttcct tatcgtgtt 
      gtgctgttg ctgcgtcac catgctgac cgccatctc 
      catgtcggc gatcgccac caacggggt ggtgcccgg 
      gggcggcag ttatttcat gatatccag gtcacttgg 
      tccagagtt tggcggcgc tgttggcat gctgtttta 
      cacagggac gacgttagc agccgcgat gtacatcgt 
      cggtgctgt tgaaattgt cttgaccta catggctcc 
      ttggttatc aatttttgg ggattttac aacaaatca 
      ggacgcaat gtacaacaa ttttcgggt ctatggaac 
      tgggttact cgtggtgat gggaactat tgtgttcgt 
      cggggtgaa gtttgtcaa caaatttgc cgccgttgc 
      tcttgcatg cgtcattct ctccatact ggctgtgta 
      cattggaat attccgcaa tatcaacgg caaagatga 
      cttgcaaat gtgcgttct tggtaaacg attgttacg 
      tgtcccggc tgctgattg ttacaaaaa cggtagtgg 
      tttcataca caacatgtt ttgtcctaa tggaaccgc 
      tagttgcga tccgtacta tgaacgtaa tgacgtaag 
      gctagttca agggattaa agggttgtc atccggtgt 
      gattttgga caatatcta cgatagctt cttggaaaa 
      tggacagta catagcaat agggaataa tcctgagga 
      tattgagcc aatggaacc tcacagaga cactgccca 
      agtgacggc agatataac aacaacttt tacaatttt 
      gatcggaat atttttccc ttcagtgac tggtattat 
      ggctgggtc taatcgttc gggtgattt agctgatgc 
      ccaaaaatc tataccaat cggaacaat ttgtgccat 
      tttaacaac gtcgactgt gtatttatc atctgtgat 
      tctcttcgc tgctactgt cgacaacct tctacttag 
      agataaatt tggctcatc tataggagg taaattagt 
      tgtagccaa catggcgtg gccaaacga atgggtcat 
      tttaatcgg atcattcct ttcgaccct tggcgctgg 
      tctccagtc actgactgg agctcctcg cttactcca 
      agccatcgc caaagacgg tatcattcc atttttatc 
      accgtttgc cgtatcatc aagccgtgg cgagccaac 
      ccgagcatt attattaac tattgtcat ttgtcagtg 
      tggtatatt attaggaaa tgttgatct tttggcgcc 
      cttactctc gatgttttt cttaatgtg ctacggttt 
      tgtaaatct cgcttgcgc tttacaaac attactacg 
      aacacccaa ctggagacc aagatttaa atattacca 
      ttggtccct ctcatttat cggcttatc gttgtgcat 
      agcaataat gtttatgac ctcttggta ctttgcgct 
      tctcgccat gggaatggc aggcttgat ctataaata 
      catcgaata cagaggtgc cgagaaaga atggggtga 
      cggcattcg aggtctagc gttgtcagc agcgcgata 
      ttcattact ccgcctaga agagggtcc tccccacac 
      caaaaactg gagacctca gctcctcat tctcgttaa 
      attaacaac tgatctcgt tccgaaata cagaaaatt 
      atttgcttt cgcgtcgca actcaaagc tgggaaggg 
      tttgacagt ttgcgctgc tgtaattgg cggcgattt 
      tacacgatc gtacggaga agcgatggc agctaaaca 
      aagcttgag aaaaaccat ggacgaaga acgggtcaa 
      gggttttgt cgatattct agtggcacg caacattac 
      cgaaggatt atctaactt agtgcaaac ttctggtct 
      tggtggcat gaagccgaa tacggtgat tttaggatg 
      gccgtatgg ctggcgaca gtcggaaga tgagcgcac 
      gtggcaggt tttcttgca aactgttcg gaatgtgac 
      agccgcaca catggcgtt attagttcc taaaggtat 
      caacttttt cccggactc tacggagaa agtcttcgg 
      gaatatcga tatctggtg gattgttca tgacggtgg 
      tcttctcat gttgttacc cttccttct caaacagca 
      ccgcacttg gaaaaattg caagatgcg cattttcac 
      tgttgctca aatggaaga caattccat ccaaatgaa 
      gaaagatct aaagacgtt cttgtatca cctccgaat 
      tgaagcaga agttgaagt cgttgaaat gatggacag 
      cgatatttc ggcatacac ctacgaaag gactctgat 
      gatggaaca gcggaatca aatgttgcg agaacttcg 
      tctcaacaa aaaagaaag tttgggtgt ggttcagtc 
      aattgtaga tcaacacca tcaaaatat cgatgctaa 
      aacagccac caaagttag gttccagga acctagcgc 
      tgaaggcga taaagataa caaagcggc tgaaacgga 
      accgacaaa cgagcctaa ttcacagac gaaacccga 
      ggataacga taattcaac aacagagaa agaagatcc 
      gtcggagaa agaatgcaa tgaggaaac aaaagagaa 
      caatatctc ctccgaaga gaaaaagaa acccgcaac 
      gataacccc tgacgaagg caacgtccg tcggatgca 
      tacagctgt aaaattaaa cgaagtgat agtgaatcg 
      ttctcacga agctcagct tgtgatctt gaatttacc 
      cggccctcc aaaagacac caagatgga aagagaatc 
      caattatat ggaattctt ggaagtgct cactgaagg 
      tttggaacg tgtgctcat ggtccgtgg tggcggtca 
      agaggtcat aactatcta ttcgtaact taaatatgg 
      gattagatt tcgaaattg gagtgtcag ttttgtatt 
      gtctgacag ccaagcagt taatcaaag gacattgct 
      atggaaatg gcgatagcc gaacagtat ttaattata 
      aaatgttac cacgtctgg atagtgatt atgccaaag 
      agatactta tttattctt ctcaatgtt atagattgt 
      atgagcggt gaagttgga ggatgtaca ggtagaatg 
      atggtaatc gtttttctg taccgtctc ctgctggaa 
      ccgacgtga ttctctatc ctatgtaat actgtatca 
      aatcaaaat gatgttccg cgaagtgct cacttttct 
      atgagaacg gctgggcca tgccaaatg ccacttgag 
      tgcatgcag tgttatgat agggcgtag cgtaagact 
      tggtagaat agcctaata ctgctaaca ctaacgagt 
      gtattcatg tagtttcgt tagttctgt tatatatca 
      tattacatt taatgtcca gcgaatttc ttttacact 
      caactaata tcgatactt agagtgtca ttttataaa 
      ttcagtaaa cataaagtg agtgtgtac ttaagtacg 
      ctatcatct cagtctcat taggtggta aaatacgcg 
      tatatggta tatactata agaacatta ctgagcaat 
      attaactaa taattactg tattaaatg tcaaattaa 
      ttatgaagt gataggata ttcacatgt acaatctat 
      accgaccgc cggagggcg ggctctccg tgctgtgac 
      aggtgacag tgctcactc acgatcagt tctcttgca 
      tgctgcgac cgactctgt ctcgtgcgg cctgccgta 
      gagataata gtgcactag tgatctcaa ctagcctta 
      aaggtacct tgaaatatt aaattctat ttatttata 
      tctatgtgt cactaaatt agctgctgc cagattacg 
      ccattctac atcaaccac ttatgactg ttggtgccc 
      gcgtcccag tggcgttca aatagaatt tatttattg 
      tcatccgag ctagtcgtt atcactttt atacgttcg 
      attattatc atttctacc caatctcca tctatttac 
      cattattgt aaatggcgc aatctgttt atcttatta 
      ttgacaatg tgttcattg gataaaaat attaaataa 
      aattacata gtatag