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Gene information
General information
TC005168 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4627520..4629254
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC005168
    iBeetle screen iB_00810

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005168 RA
      MLVLNRFAA ISRKLNTVF HAKVAFHLS HRLKNENKN 
LYDEILTED REFLAFSEN YFKFESGRN CYIIQPYIK 
WGPQKLQGI TPIEQLDEA KALVNTLPS WNIQDSISV 
PLESFDKKT LFKSGSLDK LRQIIRKNS TINAVFVNL 
GTLKKAQIV SLEREFGVP ILDRYKVVM YILKLHVVS 
KHAKLQVAL AELYYLQRS TFHEANFGK AEREKVKLM 
FQTREQKLK NAIKELRTE RALLRNKRQ KIDFPVVAV 
VGYTNAGKT SLIKALTGE ERLRPKNQL FATLDVTLH 
AGLLPSGMK VLYVDTVGF ISDIPTNLI ECFVATLED 
AVLADVILH VEDISSSFF EFKRKHVLA TLQDLATNA 
GTGDVLNKV ISVGNKCDL VPKETDFGI LKVSSKDDT 
GLDVLRMTL EKAILENTN RAKIKIRVP VGGDAIRWF 
LSSLM
    CDS sequence
    • >TC005168 RA
      atgctagtc ctcaatcgt tttgccgca atttcacgg 
aaactgaac acggttttt cacgcaaaa gtagctttc 
catctctcc caccgtcta aaaaacgaa aataaaaac 
ctatacgac gaaatttta accgaagac agagagttt 
ttagcgttc tctgaaaat tatttcaaa ttcgaatct 
gggcgaaac tgctacatt atccaaccg tacatcaag 
tggggccct cagaaactg caaggaatc acccccatt 
gaacaacta gacgaagcc aaagcccta gtcaacaca 
ctcccttcc tggaacatt caagactca atttccgta 
ccgctagaa tcatttgac aaaaaaact ctcttcaag 
tcgggttcc ttagataag ttgcgccag atcattcgc 
aaaaactcg accatcaat gccgttttc gtcaatctg 
gggaccctc aaaaaggcc caaatcgtc tcgttggag 
agggagttt ggggtcccg attctagac cgctataaa 
gtcgtcatg tacatatta aaactccac gtggtttcg 
aaacacgcc aaactccaa gtcgcccta gctgagctt 
tattatttg caacgatcc acgtttcac gaggccaat 
ttcgggaaa gctgagcgg gaaaaagtc aagctcatg 
tttcaaacg cgcgagcaa aaactcaaa aacgctatt 
aaggagttg cgcaccgaa agggccttg ttgcggaac 
aaaaggcaa aaaattgat tttccggta gttgccgtg 
gtggggtac acaaacgcc gggaagacg tcgctcatt 
aaggcgctc acaggggag gagagacta aggcccaaa 
aaccagctt tttgccact ttggatgtt acgctccac 
gcggggctg ctgccctcg gggatgaag gttttgtat 
gttgataca gtcggcttc atttcggat attccgacg 
aatttaatt gagtgtttt gtggccact cttgaagac 
gcggtcttg gcagacgtc attttgcac gttgaggat 
atttcaagc tcgtttttt gaatttaag aggaaacac 
gttttggcc actttgcaa gacctggct accaatgct 
ggcaccgga gacgttttg aacaaagtc attagtgta 
ggaaataaa tgcgatctg gtaccgaaa gagaccgat 
tttggaatt ttgaaagtg tcgtctaaa gatgacact 
ggcctagac gtccttaga atgacgttg gagaaggca 
attctggaa aacaccaac cgggcaaaa atcaaaatt 
cgagttcct gttggtggt gacgcaata agatggttt 
ttgtcatcg ctaatgtga
    mRNA sequence
    • >TC005168 RA
      cttgtacta cttttaaac cattatttt tgttactga 
taaatagaa atgagttaa ttaagtgtt ggaggttac 
ttagaagac agagtttta ttaaaagga gcaattcaa 
cagttctat tacccagtt ttaagcaaa gcgtcagcg 
ctccactgt aaccattcg gattttttc cacaaagtc 
gggcgccaa tttcaaata tgctagtcc tcaatcgtt 
ttgccgcaa tttcacgga aactgaaca cggtttttc 
acgcaaaag tagctttcc atctctccc accgtctaa 
aaaacgaaa ataaaaacc tatacgacg aaattttaa 
ccgaagaca gagagtttt tagcgttct ctgaaaatt 
atttcaaat tcgaatctg ggcgaaact gctacatta 
tccaaccgt acatcaagt ggggccctc agaaactgc 
aaggaatca cccccattg aacaactag acgaagcca 
aagccctag tcaacacac tcccttcct ggaacattc 
aagactcaa tttccgtac cgctagaat catttgaca 
aaaaaactc tcttcaagt cgggttcct tagataagt 
tgcgccaga tcattcgca aaaactcga ccatcaatg 
ccgttttcg tcaatctgg ggaccctca aaaaggccc 
aaatcgtct cgttggaga gggagtttg gggtcccga 
ttctagacc gctataaag tcgtcatgt acatattaa 
aactccacg tggtttcga aacacgcca aactccaag 
tcgccctag ctgagcttt attatttgc aacgatcca 
cgtttcacg aggccaatt tcgggaaag ctgagcggg 
aaaaagtca agctcatgt ttcaaacgc gcgagcaaa 
aactcaaaa acgctatta aggagttgc gcaccgaaa 
gggccttgt tgcggaaca aaaggcaaa aaattgatt 
ttccggtag ttgccgtgg tggggtaca caaacgccg 
ggaagacgt cgctcatta aggcgctca caggggagg 
agagactaa ggcccaaaa accagcttt ttgccactt 
tggatgtta cgctccacg cggggctgc tgccctcgg 
ggatgaagg ttttgtatg ttgatacag tcggcttca 
tttcggata ttccgacga atttaattg agtgttttg 
tggccactc ttgaagacg cggtcttgg cagacgtca 
ttttgcacg ttgaggata tttcaagct cgttttttg 
aatttaaga ggaaacacg ttttggcca ctttgcaag 
acctggcta ccaatgctg gcaccggag acgttttga 
acaaagtca ttagtgtag gaaataaat gcgatctgg 
taccgaaag agaccgatt ttggaattt tgaaagtgt 
cgtctaaag atgacactg gcctagacg tccttagaa 
tgacgttgg agaaggcaa ttctggaaa acaccaacc 
gggcaaaaa tcaaaattc gagttcctg ttggtggtg 
acgcaataa gatggtttt tgtcatcgc taatgtgat 
aataaccca gggaaaatt ggagcaatt c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY