Status
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Gene information
General information
TC005163 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4565093..4568116
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005163
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00808

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 5 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005163 RA
      MDIAKSIFS HRDREGYVG KEDHKKREI ELAISEDDV 
      DDYFIENFS EGLDMNDLL TPSPGGPFS ALTPSMWPQ 
      DIMAKLSVV PDDPNSQPE YRFDEFGFR VDEEDGPEQ 
      NSNKLLGIP FVEDPQDRL QWVAHLEFS HNKEVSDLT 
      WDKVEVKLP RTDKLRSMV RAGIPHSLR PQMWMRMSG 
      ALEKKQQSE LCYKDIVKM SSNDSLMTS KQIEKDLLH 
      TMPTNTCFS HINSTGIPR LRRILRGIA WLYPDIGYC 
      QGTGMIAAS LLLLLEEEE AFWIMVTIV EDLLPASYY 
      SSTLLGIQA DQRVLRTLI TNFLPDIDE TLKNHDIEL 
      SLITLHWFL TLFASAVHM KILLRIWDL FFFEGSIVL 
      FQVTLSMLK MKEPQLKQL ENSAQIFNA LSDIPGDID 
      DVEKLFEVS QTLTTSLNE VMIETYRRR HLAYLMADQ 
      GALVGNPEA APNLPKQHL ARRQVKKNK SVIQLLLFN 
      ENTEDDIKC KNIKQTEIL VDLREAILQ IARHFLQVD 
      PKLSTEISL VADYSTESH ATDHDNYIN VSKNRKRRA 
      KALLDFERH DDDELGFRK NDIITIISQ KDEHCWVGE 
      LNGLRGWFP AKFVQLLDE RSKQYSSAG DDSISETVT 
      DLVRGTLCP VIKQVLEHG MKRPNFLGG PCHPWLFIE 
      EAATKEVEK DFNSVYSRL VLCKTYRLD EDGKVLTPE 
      ELLYRCVQS VNLSHDNAH AQMDVKLRS LICMGLNEQ 
      VLHLWLEVL CSCSEVVQK WYHPWSFVY SPGWVQIKC 
      DLRILSQLS FNLNPDWEL PVKKETNNQ PLKDGVRDM 
      LVKHHLFSW DI
    CDS sequence
    • >TC005163 RA
      atggatatt gcaaaatcc attttcagt cacagagac 
      cgcgagggt tacgtgggc aaagaagat cacaagaaa 
      cgtgaaatt gagttggca atttcagag gacgatgtt 
      gatgattac tttattgaa aatttctct gagggccta 
      gacatgaac gacttgctt acacctagc cccggcggt 
      ccattttcg gccctgacc cctagcatg tggcctcaa 
      gacatcatg gcaaaatta agtgtagtt cctgacgac 
      cccaattcc cagccagaa tacagattt gacgaattt 
      gggttccgc gttgacgaa gaggacggt cctgagcaa 
      aattctaac aagttgttg ggtattccc tttgtggag 
      gacccccag gataggctt caatgggtt gctcattta 
      gaattctct cataataag gaagtgtcg gatttgact 
      tgggataag gttgaggtg aaattgccc agaactgac 
      aagttgcgc tcaatggtc agagcgggg attccccac 
      tctttgagg ccccaaatg tggatgcga atgtcaggg 
      gcgttagaa aagaaacaa cagtcggaa ttgtgctac 
      aaagatatc gttaaaatg tccagcaac gattcttta 
      atgacctcg aagcaaatt gaaaaagat ttgttgcac 
      acaatgccg acaaatacg tgttttagt cacattaac 
      agcactggg attcccaga ttgagacgg attttgcga 
      ggaatcgct tggctttat ccagatatt gggtattgt 
      caagggact ggcatgatc gccgcgtcg cttcttctt 
      ctacttgaa gaagaagaa gcgttttgg atcatggtg 
      acaatagtt gaagatctc ttacctgcc tcttactac 
      agctcaact ttgttaggg attcaagct gaccaaaga 
      gttttgcgc acattaatc acgaatttt ttgcccgat 
      atcgacgaa actctgaaa aaccatgac atagaacta 
      tctctaata actctacac tggtttcta acgctcttt 
      gccagcgct gtccatatg aaaatcttg ctaagaatc 
      tgggactta tttttcttc gaaggatct atagtatta 
      tttcaagta acgttaagc atgttgaaa atgaaagaa 
      cctcagctg aagcaactc gaaaattct gctcaaatt 
      tttaacgca ttgtccgac attcctgga gatatagac 
      gacgtagag aaactgttt gaagtgtct caaacgttg 
      acgacctct ttgaacgaa gtcatgata gagacttac 
      aggcggcgt cacttggcc taccttatg gcggaccaa 
      ggggcctta gtaggcaac ccggaggcc gcccccaac 
      ttgcccaaa cagcacctc gccaggcgc caggtcaag 
      aaaaacaaa tccgttatc caactcttg ctcttcaac 
      gaaaacact gaagacgac atcaagtgc aaaaacatc 
      aaacagacg gagattctg gtagacttg cgagaggcg 
      attttacag atcgcgcgt cattttctg caagtggat 
      ccgaaattg agcactgaa atttccctg gttgcggac 
      tattcaacg gaaagtcat gcaaccgat cacgacaat 
      tacataaac gtttcgaaa aatcgcaaa cggcgggct 
      aaggctcta ttagatttc gagcgtcat gatgacgac 
      gagttgggt tttcgtaaa aacgacatt attacgatt 
      ataagtcag aaggatgag cactgttgg gtgggggaa 
      ctgaacgga ttgagaggg tggtttccg gcaaaattt 
      gtccagtta ttggacgaa agaagtaaa caatacagt 
      agcgccggg gatgatagc atatccgaa acagtgact 
      gacttagtc agggggacc ttatgccca gttattaag 
      caagtatta gaacacggg atgaaaagg cctaacttt 
      cttggtgga ccttgccat ccttggctt ttcatagaa 
      gaagcggca acgaaagag gttgaaaaa gactttaat 
      tcggtgtat agtaggcta gttctttgc aaaacatac 
      agactggac gaagacgga aaagttcta actccggaa 
      gaattgctg taccgctgc gtccagtcg gtcaacctg 
      tctcatgac aatgcgcac gctcaaatg gatgttaaa 
      ctgcgctcg ttgatttgt atgggtttg aacgagcaa 
      gttttacac ttatggctc gaagtccta tgttcttgc 
      agcgaagtt gttcagaaa tggtaccat ccgtggtcg 
      ttcgtttac agcccgggt tgggtgcag atcaaatgt 
      gatttacga attttatcg caactgtcg tttaatttg 
      aaccccgac tgggagctt cccgttaaa aaagaaacc 
      aataaccaa ccactgaag gatggtgtt cgcgatatg 
      ttagtcaaa catcatctc ttcagttgg gacatctga
    mRNA sequence
    • >TC005163 RA
      aagcgtcaa aatttgttg tccatatgt tgttgttaa 
      cacaaatag ataatttat taatttatg ttgtattga 
      ccgttctta tctaaaatg aaaatttac ttttataaa 
      agtgttaca accatatga ccggttaca ctggtagcg 
      aaaatctga tttgttgtt aaaaatctg tgaaaaaat 
      ggatattgc aaaatccat tttcagtca cagagaccg 
      cgagggtta cgtgggcaa agaagatca caagaaacg 
      tgaaattga gttggcaat ttcagagga cgatgttga 
      tgattactt tattgaaaa tttctctga gggcctaga 
      catgaacga cttgcttac acctagccc cggcggtcc 
      attttcggc cctgacccc tagcatgtg gcctcaaga 
      catcatggc aaaattaag tgtagttcc tgacgaccc 
      caattccca gccagaata cagatttga cgaatttgg 
      gttccgcgt tgacgaaga ggacggtcc tgagcaaaa 
      ttctaacaa gttgttggg tattccctt tgtggagga 
      cccccagga taggcttca atgggttgc tcatttaga 
      attctctca taataagga agtgtcgga tttgacttg 
      ggataaggt tgaggtgaa attgcccag aactgacaa 
      gttgcgctc aatggtcag agcggggat tccccactc 
      tttgaggcc ccaaatgtg gatgcgaat gtcaggggc 
      gttagaaaa gaaacaaca gtcggaatt gtgctacaa 
      agatatcgt taaaatgtc cagcaacga ttctttaat 
      gacctcgaa gcaaattga aaaagattt gttgcacac 
      aatgccgac aaatacgtg ttttagtca cattaacag 
      cactgggat tcccagatt gagacggat tttgcgagg 
      aatcgcttg gctttatcc agatattgg gtattgtca 
      agggactgg catgatcgc cgcgtcgct tcttcttct 
      acttgaaga agaagaagc gttttggat catggtgac 
      aatagttga agatctctt acctgcctc ttactacag 
      ctcaacttt gttagggat tcaagctga ccaaagagt 
      tttgcgcac attaatcac gaatttttt gcccgatat 
      cgacgaaac tctgaaaaa ccatgacat agaactatc 
      tctaataac tctacactg gtttctaac gctctttgc 
      cagcgctgt ccatatgaa aatcttgct aagaatctg 
      ggacttatt tttcttcga aggatctat agtattatt 
      tcaagtaac gttaagcat gttgaaaat gaaagaacc 
      tcagctgaa gcaactcga aaattctgc tcaaatttt 
      taacgcatt gtccgacat tcctggaga tatagacga 
      cgtagagaa actgtttga agtgtctca aacgttgac 
      gacctcttt gaacgaagt catgataga gacttacag 
      gcggcgtca cttggccta ccttatggc ggaccaagg 
      ggccttagt aggcaaccc ggaggccgc ccccaactt 
      gcccaaaca gcacctcgc caggcgcca ggtcaagaa 
      aaacaaatc cgttatcca actcttgct cttcaacga 
      aaacactga agacgacat caagtgcaa aaacatcaa 
      acagacgga gattctggt agacttgcg agaggcgat 
      tttacagat cgcgcgtca ttttctgca agtggatcc 
      gaaattgag cactgaaat ttccctggt tgcggacta 
      ttcaacgga aagtcatgc aaccgatca cgacaatta 
      cataaacgt ttcgaaaaa tcgcaaacg gcgggctaa 
      ggctctatt agatttcga gcgtcatga tgacgacga 
      gttgggttt tcgtaaaaa cgacattat tacgattat 
      aagtcagaa ggatgagca ctgttgggt gggggaact 
      gaacggatt gagagggtg gtttccggc aaaatttgt 
      ccagttatt ggacgaaag aagtaaaca atacagtag 
      cgccgggga tgatagcat atccgaaac agtgactga 
      cttagtcag ggggacctt atgcccagt tattaagca 
      agtattaga acacgggat gaaaaggcc taactttct 
      tggtggacc ttgccatcc ttggctttt catagaaga 
      agcggcaac gaaagaggt tgaaaaaga ctttaattc 
      ggtgtatag taggctagt tctttgcaa aacatacag 
      actggacga agacggaaa agttctaac tccggaaga 
      attgctgta ccgctgcgt ccagtcggt caacctgtc 
      tcatgacaa tgcgcacgc tcaaatgga tgttaaact 
      gcgctcgtt gatttgtat gggtttgaa cgagcaagt 
      tttacactt atggctcga agtcctatg ttcttgcag 
      cgaagttgt tcagaaatg gtaccatcc gtggtcgtt 
      cgtttacag cccgggttg ggtgcagat caaatgtga 
      tttacgaat tttatcgca actgtcgtt taatttgaa 
      ccccgactg ggagcttcc cgttaaaaa agaaaccaa 
      taaccaacc actgaagga tggtgttcg cgatatgtt 
      agtcaaaca tcatctctt cagttggga catctgaac 
      tgtaaatat attctgtcc ataacg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY