Status
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Gene information
General information
TC031795
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005137,TC005136
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450232.1: 175897..200678
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031795
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00807

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 10 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    delayed (still prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031795 RA
      MVFATLLVV SLLFAGTLQ FQFKHHNND ELVQVLQDV 
      NSRCPNITR VYTLTETSV LGLPLYLIE FSTKPGHHE 
      IMKPEFKYI ANMHGNEVL GRELLLKLA DYLCEQYTA 
      GNPEIQSLI EQTRIHLMP SMNPDGWQL ATDTGGKDY 
      LIGRTNNNS IDLNRNFPD LDRIMFSNE ENHIDHNNH 
      LLAQLTRLK EPLQPETKA VIRLIMQIP FVLSANLHG 
      GDLVANYPY DESKTERQK DEYSKSPDD ETFRHLALA 
      YSTHHVDMA NPTRGGCGD AHSDRFGKQ GGITNGAKW 
      YSLAGGMQD FNYLSSNDF EITLELGCD KYPPAYTLQ 
      HEWERNKNA LINFIWQSH IGIKGIVYD SVLNRGIAN 
      AIIHVKNIT GGQVRDVQH DVTSVHDGD YFRLLTPGQ 
      YKITAYKDG YLPHSRLVT VTNIPHSSA QRVDFGLKP 
      ISVPPYVRI QANNFIDEK VDNYENMQE PWTNWVNMN 
      RQPIAVE
    CDS sequence
    • >TC031795 RA
      atggtgttc gcaacattg ttggtcgtg tccctgctc 
      ttcgcgggg acactgcag ttccaattc aaacaccac 
      aataacgac gaattggtg caagtgctg caagatgtc 
      aacagcagg tgtccgaat atcacgagg gtctacaca 
      ctgacggag accagcgtt ttggggctt cccttgtat 
      ctgatcgag ttttccacc aagccagga catcacgaa 
      attatgaaa cccgaattc aaatacata gcaaacatg 
      cacggtaat gaggttttg ggaagagaa cttttatta 
      aagttagct gattacctc tgcgaacaa tacactgct 
      ggcaacccc gaaattcag tctcttata gaacagacc 
      cgaattcat cttatgcct tcaatgaat ccagacggc 
      tggcagctt gctactgac acgggaggc aaagactat 
      ttaatagga cgcactaat aacaattcg attgatttg 
      aatcgaaat tttcccgat ttggataga atcatgttc 
      agcaatgaa gaaaaccat atcgatcac aataatcat 
      cttcttgca caattgaca aggctgaag gaaccgctt 
      cagccggaa acgaaagcc gtaattcga cttatcatg 
      caaattccg ttcgtgctt tccgccaac cttcacggg 
      ggtgacttg gtcgctaat tatccgtac gatgagagt 
      aaaaccgaa agacaaaag gacgagtat tctaaaagt 
      ccggatgat gagacattt aggcattta gctttggct 
      tattcgact catcatgta gatatggca aatccgaca 
      aggggagga tgcggtgat gctcacagt gaccggttt 
      gggaaacaa gggggaatt accaatggg gctaaatgg 
      tacagcttg gctggagga atgcaggat ttcaattat 
      ctgtcgagt aacgatttt gagataact ttggaactc 
      ggctgtgac aagtaccca ccggcgtac actcttcag 
      catgaatgg gaaaggaac aagaatgca ttgattaat 
      ttcatttgg caaagtcat attggtatc aaaggaatt 
      gtttatgac agtgttttg aatagggga atagcaaat 
      gcgattatt catgtcaag aatatcacg ggtggacaa 
      gttagagat gtgcaacat gatgttact tcagttcac 
      gatggtgac tactttcgc cttctcaca cctggccaa 
      tacaaaatc acagcctat aaagacgga tatttgcct 
      cattccaga ctagtaaca gtcacgaac attccccat 
      tcatctgct caaagagtc gacttcggg cttaaacca 
      atttcggta ccaccctat gtgagaatc caagcaaat 
      aattttatc gatgagaaa gtagataac tacgaaaac 
      atgcaagaa ccgtggaca aactgggtt aatatgaac 
      aggcaaccg atagcagtt gaataa
    mRNA sequence
    • >TC031795 RA
      cgccattga taaacaaac taagcgtct tccgtcgag 
      gagaaaatc catattgtc gtcgtttac gtcaagcgc 
      cggcgtagt gacgcttct tcatttcga cgctcgtcg 
      acgaccatt cacaacagc tggagctgt tatggtgtt 
      cgcaacatt gttggtcgt gtccctgct cttcgcggg 
      gacactgca gttccaatt caaacacca caataacga 
      cgaattggt gcaagtgct gcaagatgt caacagcag 
      gtgtccgaa tatcacgag ggtctacac actgacgga 
      gaccagcgt tttggggct tcccttgta tctgatcga 
      gttttccac caagccagg acatcacga aattatgaa 
      acccgaatt caaatacat agcaaacat gcacggtaa 
      tgaggtttt gggaagaga acttttatt aaagttagc 
      tgattacct ctgcgaaca atacactgc tggcaaccc 
      cgaaattca gtctcttat agaacagac ccgaattca 
      tcttatgcc ttcaatgaa tccagacgg ctggcagct 
      tgctactga cacgggagg caaagacta tttaatagg 
      acgcactaa taacaattc gattgattt gaatcgaaa 
      ttttcccga tttggatag aatcatgtt cagcaatga 
      agaaaacca tatcgatca caataatca tcttcttgc 
      acaattgac aaggctgaa ggaaccgct tcagccgga 
      aacgaaagc cgtaattcg acttatcat gcaaattcc 
      gttcgtgct ttccgccaa ccttcacgg gggtgactt 
      ggtcgctaa ttatccgta cgatgagag taaaaccga 
      aagacaaaa ggacgagta ttctaaaag tccggatga 
      tgagacatt taggcattt agctttggc ttattcgac 
      tcatcatgt agatatggc aaatccgac aaggggagg 
      atgcggtga tgctcacag tgaccggtt tgggaaaca 
      agggggaat taccaatgg ggctaaatg gtacagctt 
      ggctggagg aatgcagga tttcaatta tctgtcgag 
      taacgattt tgagataac tttggaact cggctgtga 
      caagtaccc accggcgta cactcttca gcatgaatg 
      ggaaaggaa caagaatgc attgattaa tttcatttg 
      gcaaagtca tattggtat caaaggaat tgtttatga 
      cagtgtttt gaatagggg aatagcaaa tgcgattat 
      tcatgtcaa gaatatcac gggtggaca agttagaga 
      tgtgcaaca tgatgttac ttcagttca cgatggtga 
      ctactttcg ccttctcac acctggcca atacaaaat 
      cacagccta taaagacgg atatttgcc tcattccag 
      actagtaac agtcacgaa cattcccca ttcatctgc 
      tcaaagagt cgacttcgg gcttaaacc aatttcggt 
      accacccta tgtgagaat ccaagcaaa taattttat 
      cgatgagaa agtagataa ctacgaaaa catgcaaga 
      accgtggac aaactgggt taatatgaa caggcaacc 
      gatagcagt tgaataaat attttttgg aaatttctt 
      taagttttt taggtctag gatgaacca aactcttta 
      atataataa aaaagatta tttttgtta ttttggagt 
      agtcaaatt agttagaat tgatggttt ataaaacac 
      ataaagatt tataaaatt taaatatgt tcttatgta 
      caaaaaatg taaaatgtt gtgtatact gtgattatt 
      tatttaaaa taaaatttt agtccactc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY