Status
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Gene information
General information
TC030948
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005131,TC005132
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450232.1: 86090..110436
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030948
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00806

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number no/almost no eggs -
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030948 RA
      MFPFLIKSL LLSIVVGAN DTLCPTKCV CKRVNQRDE 
      LKLRCGEPD NKILSFEEI DLLNIANDI VQFNLSNNL 
      LDTFFPKVQ LIALQKLDL SKNRLTELQ DNQFAELPN 
      LRRLDISGN NIKSIQLLA FAKLGSLER LKLNQNQIS 
      TIALGTFNP LVSLKQLDI SNNPLTCDC SLLWLLDWS 
      QKESVKLLS NPTCNTPPS FKNLPLRKL KIGVDIHCK 
      SKALSKGFP IIEMKPSVN QVVFEGDPL KLQCTAPSI 
      SDTYDSPLS SKIEWTWLD SDPKLYFSD ITIENHFLQ 
      STGLVVSTL QIAKLKRNH TGIWTCLLL SMQGNHSKG 
      ITIVVISDE TEYCPITVT SNNKGTYTW PRTVVNYTA 
      TVPCESPNL SYDVSLHKA SYFCSVEGQ WENLNTSAC 
      SYTSETTKI LEQFSKVNS SIVESAKHF KNYTSVAEH 
      FKDEMDIVF AVETMENYL RYLTVNQIG GVLMDVTNN 
      LLNLPKEYL RWADYRYGS CRKLVNISE KLAGISTTA 
      LLHKSNMAT EIFPVKRNG FSSLKCTWY TDIQKETER 
      LFYCVTNSN SDTTELPGK TIEASITIP ETLFEQLED 
      QNYDLSDKI YNILISVYS SNKFFPIDE KRENQVDVT 
      SPVVGAKLG NEFTHFVVT NLTDPIFVM LKIPSEEGV 
      LTPVWWDNG TWSPDSCHF SHNYQDHVV FYCHNFGYY 
      GLLQDVSNL QNSIIGAKF KFSHPAIYV GCFVLFTSL 
      LVVIVIYLF AYASIQMPK KAKHSLINL WISVCLLCF 
      LYIFGIYQT EDVRVCQIV GLCLHYLTL SSLLWMCVG 
      VNCMYKRLS KNDVLELQD DELVSEPPV KKSIIGLYF 
      IGWGVALIV CGISAAINI KDYASPTYC FLRSGPPLS 
      ALYIPFTIL FLFLCIYFL LIRCAIYGL DANGHLSEG 
      TQATENVDL DLLEPNFPE TRSVVSESS SSEVEDPEH 
      APSVQLKSY IIFLFLYMF TWVCCAFAT VEPAFISYQ 
      NDVFSVSFA ILAFVLSVF TVFFYCIAR SDVRGLWVQ 
      LRRKQLCFR SRNVSDTVQ NLPNSVEIV SRSSSRSSS 
      RTKSSNLKA AVDLNGYSS DGAKINNVN LIVLHRQQY 
      RTTIPNIIE NPTSAAEVF YNPHQSIVA RKFFRRQKR 
      HMMKRNNLV PSVKKDNSS NDQSIYGTN SKVNNTNIH 
      VERVRKKQK NPNLFSDSG DECEIVLKK ARKSKTQSE 
      NNMRSVSQQ CTLEYSSEN ISDSILDKT SPDKECEKN 
      DKVPTIYVN PSHDLTVRK RVQSRASSV SVSDLDELY 
      QQIRRGPIV NSFNVTRNS PCFSDSDIT DQYHREQTY 
      LSDNVETTV
    CDS sequence
    • >TC030948 RA
      atgtttcct tttttaatt aaaagtcta cttttgtcg 
      attgtagtc ggtgccaac gacactctt tgtcctaca 
      aaatgtgtg tgcaaaagg gtgaatcaa cgcgacgag 
      ttgaaacta cgttgtggc gaacctgat aataaaatt 
      cttagtttt gaagaaatt gatttgctt aacattgcc 
      aacgatatt gttcaattc aatctgtcc aataatctg 
      ctggatacc ttcttcccg aaagtacaa ttaattgct 
      ctacaaaaa ctcgattta agtaaaaac aggttaacc 
      gaattgcaa gataaccag tttgcagaa ttacccaat 
      ttaagaaga ctagacata tcaggaaat aacataaaa 
      agtatacag ttgctggct tttgcaaaa cttggaagt 
      ttagaacgg cttaaattg aaccagaat cagattagt 
      actattgct ttagggacg tttaatcca ctcgtaagc 
      ttgaaacaa ttagacata tcaaacaac cctttaaca 
      tgcgactgc agcctttta tggcttctc gactggagt 
      caaaaagaa tccgtaaaa cttctttca aaccccact 
      tgcaacacc ccaccatca ttcaaaaac cttccactt 
      cgtaaatta aaaatcggc gttgacatc cactgcaaa 
      tcgaaagct ctcagcaaa ggtttccct ataatcgaa 
      atgaaacca tccgtcaat caagtcgtt ttcgaaggc 
      gaccctttg aaactccaa tgcacagct cctagcata 
      tccgacaca tacgactca cctctaagt tcgaaaatc 
      gaatggact tggctagat tccgatccg aaattatat 
      ttctcagac ataacaatt gaaaaccat tttttacaa 
      agcaccgga ttggtcgtt agcacttta caaatcgcg 
      aaattgaag cgaaaccat acaggaatt tggacttgt 
      ttattgctt tcgatgcag gggaatcac tcgaaaggg 
      atcacaata gtggtgatt tcagacgag actgaatat 
      tgcccaatt actgtgacc tcgaacaat aaagggacg 
      tatacgtgg cctcgaact gttgttaat tacacggcg 
      acagttccg tgtgaatcc ccgaatttg agttacgat 
      gtttcgtta cataaagct tcgtatttt tgttcggtg 
      gagggccag tgggagaat ttaaacact tcggcttgt 
      tcgtatacg tccgagacg acgaaaata ctcgaacag 
      ttttccaaa gtcaattcg agtattgtg gagagtgct 
      aaacatttc aagaattat acgtcggtg gcagagcat 
      tttaaggat gaaatggat attgttttt gctgttgaa 
      acgatggag aattatttg aggtatttg acggttaat 
      cagatcgga ggggtgctt atggatgtg acgaataat 
      ttgttgaat ttaccgaag gagtatctc agatgggct 
      gattatcga tatgggagt tgcaggaag ttggttaat 
      atcagtgag aaattggca gggattagc acgacggca 
      ttgttacat aagtccaac atggctacg gaaatattt 
      cccgtcaaa agaaacggc ttttcaagc ctaaaatgc 
      acttggtac acagacatc caaaaggaa acagaacgc 
      ctgttctat tgcgtcaca aacagcaac agcgacacg 
      accgaactt cccggaaaa acaattgaa gcttccatc 
      accatccca gaaacactc ttcgaacaa ctcgaggat 
      caaaactac gacctctcc gacaaaatc tataacata 
      ctcatatca gtatactcg tcaaacaaa ttcttccct 
      attgatgaa aaacgcgaa aatcaagtt gatgtgaca 
      tcgccggta gttggcgcg aaattgggc aacgaattt 
      acacatttc gtcgtaacg aatttaacc gacccgatt 
      ttcgtcatg ttgaaaata ccatcggaa gaaggtgtt 
      ctaacaccg gtctggtgg gataatggt acttggtca 
      cccgatagt tgtcacttt agtcacaat tatcaagac 
      catgtggtt ttttactgt cacaatttc ggttattat 
      ggacttttg caagacgtt tcgaacttg cagaattcg 
      atcatcggt gccaaattt aaattttcg catccggcg 
      atttatgtc ggatgtttc gtactattc acttcactt 
      cttgtcgtt attgtaatt tatttgttt gcttacgca 
      tcgattcaa atgccgaaa aaagccaaa cactcgctg 
      atcaattta tggatttcg gtgtgtttg ctatgtttt 
      ttgtacatt tttggcatt taccaaacg gaggacgta 
      cgagtttgc caaatcgtc ggtttgtgt ctccactac 
      ttgacactg tcttcgtta ctttggatg tgcgtcggt 
      gtcaattgc atgtacaaa aggctgagt aaaaacgac 
      gttttagag ctgcaagac gacgaatta gtatcggaa 
      cctccagtc aaaaaatcc attatcggt ctctacttt 
      atcggctgg ggtgtggca cttatagtg tgcggtatc 
      tcggcagcg ataaatatt aaggattac gcatcgcct 
      acttattgt tttctacgg agcggtccg ccactcagc 
      gctctctat ataccattc acgatcctt ttcctcttc 
      ctgtgcatc tacttcctg ttgattcgt tgcgccatc 
      tatggactt gacgcgaac ggacatctc agtgaaggg 
      acccaagcc acggaaaac gtcgacttg gatttacta 
      gagccgaat tttcccgaa acacgaagt gtggtaagc 
      gaatcgagt tcgagcgaa gtcgaggat cccgaacat 
      gcgccttcg gtccaactc aaatcgtac ataattttt 
      ttatttttg tatatgttc acgtgggtt tgttgcgcg 
      ttcgcaacc gtcgaaccg gcgttcatt tcgtaccaa 
      aacgatgtt tttagtgtt agttttgcc attcttgcg 
      tttgtttta agtgtgttt actgtgttt ttctactgc 
      attgcacgg agtgatgtt cgagggttg tgggtacag 
      ttgaggcgg aaacagttg tgtttccgc agtcgtaat 
      gtttccgac actgttcaa aatctcccg aattcagtc 
      gagattgtt tcaaggtcg agtagtcgg tcctcaagt 
      cgcactaaa tcgagtaat ttgaaagca gcggttgat 
      ttaaatggg tatagtagc gacggggcg aaaataaac 
      aacgtcaat ttgattgtc ttacaccga caacaatac 
      cgaacaact ataccgaac attatcgag aacccgacg 
      agtgcggcc gaagtgttt tacaatccg catcagagt 
      atcgtggcg aggaagttt ttccggcgc caaaagcgc 
      cacatgatg aagcggaat aatctggta ccgagtgtg 
      aaaaaagac aattcgagt aacgaccag agtatttac 
      gggacgaat tcgaaagtc aacaataca aacattcac 
      gtggaacgg gtgcgcaag aaacagaaa aatccgaat 
      ttgttttcg gatagcgga gatgagtgt gagattgtt 
      ttaaaaaag gcgcgtaaa agtaaaaca cagtcggag 
      aataacatg agatcggtt tcgcaacag tgcactttg 
      gagtacagc tctgagaat atttccgat tcgattttg 
      gacaaaaca agtccggat aaagagtgc gagaaaaac 
      gataaagtt cctacgatt tacgtgaac ccgtcacat 
      gatctcacc gtgcggaaa cgagtacaa agtagagct 
      agtagtgtg tctgtgagt gatttggac gagttgtat 
      caacagatt aggaggggt ccaattgta aatagtttc 
      aacgtgact agaaatagt ccgtgtttt tcagattcc 
      gatattacc gaccagtat catagagaa cagacgtac 
      ctcagcgac aatgtcgaa acaaccgtt tga
    mRNA sequence
    • >TC030948 RA
      ttcgataaa aaatcccca tcaaaatgg ccgccgaaa 
      tgtttttat ttacattta ccataaaca gttttaaat 
      agaccgtgt taaaatggt gaatcgaat agcaaacgc 
      ttcggaaag tggcccaat attgtgata aaattagga 
      aaaatgttt cctttttta attaaaagt ctacttttg 
      tcgattgta gtcggtgcc aacgacact ctttgtcct 
      acaaaatgt gtgtgcaaa agggtgaat caacgcgac 
      gagttgaaa ctacgttgt ggcgaacct gataataaa 
      attcttagt tttgaagaa attgatttg cttaacatt 
      gccaacgat attgttcaa ttcaatctg tccaataat 
      ctgctggat accttcttc ccgaaagta caattaatt 
      gctctacaa aaactcgat ttaagtaaa aacaggtta 
      accgaattg caagataac cagtttgca gaattaccc 
      aatttaaga agactagac atatcagga aataacata 
      aaaagtata cagttgctg gcttttgca aaacttgga 
      agtttagaa cggcttaaa ttgaaccag aatcagatt 
      agtactatt gctttaggg acgtttaat ccactcgta 
      agcttgaaa caattagac atatcaaac aacccttta 
      acatgcgac tgcagcctt ttatggctt ctcgactgg 
      agtcaaaaa gaatccgta aaacttctt tcaaacccc 
      acttgcaac accccacca tcattcaaa aaccttcca 
      cttcgtaaa ttaaaaatc ggcgttgac atccactgc 
      aaatcgaaa gctctcagc aaaggtttc cctataatc 
      gaaatgaaa ccatccgtc aatcaagtc gttttcgaa 
      ggcgaccct ttgaaactc caatgcaca gctcctagc 
      atatccgac acatacgac tcacctcta agttcgaaa 
      atcgaatgg acttggcta gattccgat ccgaaatta 
      tatttctca gacataaca attgaaaac catttttta 
      caaagcacc ggattggtc gttagcact ttacaaatc 
      gcgaaattg aagcgaaac catacagga atttggact 
      tgtttattg ctttcgatg caggggaat cactcgaaa 
      gggatcaca atagtggtg atttcagac gagactgaa 
      tattgccca attactgtg acctcgaac aataaaggg 
      acgtatacg tggcctcga actgttgtt aattacacg 
      gcgacagtt ccgtgtgaa tccccgaat ttgagttac 
      gatgtttcg ttacataaa gcttcgtat ttttgttcg 
      gtggagggc cagtgggag aatttaaac acttcggct 
      tgttcgtat acgtccgag acgacgaaa atactcgaa 
      cagttttcc aaagtcaat tcgagtatt gtggagagt 
      gctaaacat ttcaagaat tatacgtcg gtggcagag 
      cattttaag gatgaaatg gatattgtt tttgctgtt 
      gaaacgatg gagaattat ttgaggtat ttgacggtt 
      aatcagatc ggaggggtg cttatggat gtgacgaat 
      aatttgttg aatttaccg aaggagtat ctcagatgg 
      gctgattat cgatatggg agttgcagg aagttggtt 
      aatatcagt gagaaattg gcagggatt agcacgacg 
      gcattgtta cataagtcc aacatggct acggaaata 
      tttcccgtc aaaagaaac ggcttttca agcctaaaa 
      tgcacttgg tacacagac atccaaaag gaaacagaa 
      cgcctgttc tattgcgtc acaaacagc aacagcgac 
      acgaccgaa cttcccgga aaaacaatt gaagcttcc 
      atcaccatc ccagaaaca ctcttcgaa caactcgag 
      gatcaaaac tacgacctc tccgacaaa atctataac 
      atactcata tcagtatac tcgtcaaac aaattcttc 
      cctattgat gaaaaacgc gaaaatcaa gttgatgtg 
      acatcgccg gtagttggc gcgaaattg ggcaacgaa 
      tttacacat ttcgtcgta acgaattta accgacccg 
      attttcgtc atgttgaaa ataccatcg gaagaaggt 
      gttctaaca ccggtctgg tgggataat ggtacttgg 
      tcacccgat agttgtcac tttagtcac aattatcaa 
      gaccatgtg gttttttac tgtcacaat ttcggttat 
      tatggactt ttgcaagac gtttcgaac ttgcagaat 
      tcgatcatc ggtgccaaa tttaaattt tcgcatccg 
      gcgatttat gtcggatgt ttcgtacta ttcacttca 
      cttcttgtc gttattgta atttatttg tttgcttac 
      gcatcgatt caaatgccg aaaaaagcc aaacactcg 
      ctgatcaat ttatggatt tcggtgtgt ttgctatgt 
      tttttgtac atttttggc atttaccaa acggaggac 
      gtacgagtt tgccaaatc gtcggtttg tgtctccac 
      tacttgaca ctgtcttcg ttactttgg atgtgcgtc 
      ggtgtcaat tgcatgtac aaaaggctg agtaaaaac 
      gacgtttta gagctgcaa gacgacgaa ttagtatcg 
      gaacctcca gtcaaaaaa tccattatc ggtctctac 
      tttatcggc tggggtgtg gcacttata gtgtgcggt 
      atctcggca gcgataaat attaaggat tacgcatcg 
      cctacttat tgttttcta cggagcggt ccgccactc 
      agcgctctc tatatacca ttcacgatc cttttcctc 
      ttcctgtgc atctacttc ctgttgatt cgttgcgcc 
      atctatgga cttgacgcg aacggacat ctcagtgaa 
      gggacccaa gccacggaa aacgtcgac ttggattta 
      ctagagccg aattttccc gaaacacga agtgtggta 
      agcgaatcg agttcgagc gaagtcgag gatcccgaa 
      catgcgcct tcggtccaa ctcaaatcg tacataatt 
      tttttattt ttgtatatg ttcacgtgg gtttgttgc 
      gcgttcgca accgtcgaa ccggcgttc atttcgtac 
      caaaacgat gtttttagt gttagtttt gccattctt 
      gcgtttgtt ttaagtgtg tttactgtg tttttctac 
      tgcattgca cggagtgat gttcgaggg ttgtgggta 
      cagttgagg cggaaacag ttgtgtttc cgcagtcgt 
      aatgtttcc gacactgtt caaaatctc ccgaattca 
      gtcgagatt gtttcaagg tcgagtagt cggtcctca 
      agtcgcact aaatcgagt aatttgaaa gcagcggtt 
      gatttaaat gggtatagt agcgacggg gcgaaaata 
      aacaacgtc aatttgatt gtcttacac cgacaacaa 
      taccgaaca actataccg aacattatc gagaacccg 
      acgagtgcg gccgaagtg ttttacaat ccgcatcag 
      agtatcgtg gcgaggaag tttttccgg cgccaaaag 
      cgccacatg atgaagcgg aataatctg gtaccgagt 
      gtgaaaaaa gacaattcg agtaacgac cagagtatt 
      tacgggacg aattcgaaa gtcaacaat acaaacatt 
      cacgtggaa cgggtgcgc aagaaacag aaaaatccg 
      aatttgttt tcggatagc ggagatgag tgtgagatt 
      gttttaaaa aaggcgcgt aaaagtaaa acacagtcg 
      gagaataac atgagatcg gtttcgcaa cagtgcact 
      ttggagtac agctctgag aatatttcc gattcgatt 
      ttggacaaa acaagtccg gataaagag tgcgagaaa 
      aacgataaa gttcctacg atttacgtg aacccgtca 
      catgatctc accgtgcgg aaacgagta caaagtaga 
      gctagtagt gtgtctgtg agtgatttg gacgagttg 
      tatcaacag attaggagg ggtccaatt gtaaatagt 
      ttcaacgtg actagaaat agtccgtgt ttttcagat 
      tccgatatt accgaccag tatcataga gaacagacg 
      tacctcagc gacaatgtc gaaacaacc gtttgagat 
      taattgaga ttattttta cttatcgct gacctatcg 
      tattcaatg tttgtgctg ttgttgccg aaaatacgt 
      aatagcggc aggtattgc aggtttctt tattgccgt 
      gaaatgctc aatatttag tttctcgta ttagctagg 
      taaagatat attttttgt atgcgattt atggaatat 
      tatacatcg aaatgtgca ttattagta caattaaga 
      attatttat ttgctgaac gtgtgcact gtttcaatt 
      ttagaatag aactgattt atgtcgacg aaataccta 
      cctgtgata tatttatat tgaaaaaat accacaacc 
      taattattt ctgtttggt agtgacaca gaatgtttt 
      taaatcagt tttacttct agaagacct attacatgt 
      gaagctttt gtaaattat ttttaaaaa gcatgtatt 
      tgaaattat gatgtcatt tgatgttga tgcaaaggc 
      agtctgttg taagtatta aaaaaaaga gtacaatta 
      tgtacaata ttataaaga ctgttagct attaaaaga 
      atatacaaa atgctaaaa g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY