Status
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Gene information
General information
TC005099 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 1695667..1708737
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005099
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00804

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005099 RA
      MADSNSLLE DGQSLQDMM NDGVGLTYN DFIILPGYI 
      DFTPDAVDL TTQLTKKIT LKSPLVSSP MDTVTESSM 
      AIAMALCGG IGVIHHNCS PSYQANEVL KVKKYKHGF 
      IHNPVVLCP TNTVADVLK TKKEQGFSG IPITEDGKM 
      GGKLVGIVT SRDLDFLED QNYSNSKLE TIMTKLEDL 
      VTAQSGVTL PEANSILAK SKKGKLPIV NAEGNLVAL 
      MARTDLKKA KSYPTASKD DNKQLIVGA AIGTRDEDK 
      ERLKLLVNA GVDVIVLDS SQGNSVYQI EMIKYIKKS 
      YPSLQVIAG NVVTAKQAK NLIDAGADA LRCGMGSGS 
      ICITQEVMA VGRAQATAV YRVAQYAKR YGVPVIADG 
      GIQSIGHII KALALGASS VMMGSMLAG TSEAPGEYY 
      FSDGVRLKK YRGMGSIEA MNRKDAMGS AMNRYFHSD 
      ADKLKVAQG VSGSIVDKG SVFRFVPYL QCGIRHGCQ 
      DIGVKSLTK LKELIDCGE VRFEKRTHS AQLEGNVHG 
      LFSYEKRLF
    CDS sequence
    • >TC005099 RA
      atggctgac agcaacagt ttgcttgaa gatggccaa 
      tcactccaa gacatgatg aacgatggc gttggactc 
      acttacaat gactttata atcctccca ggatatatc 
      gattttaca ccggacgca gttgacttg acgacccaa 
      ttgaccaaa aaaataacg ctcaaatcg ccgcttgtt 
      tcgtctccg atggacact gtgacagaa tcgtcaatg 
      gcgattgca atggcgctt tgtggtggg attggggtg 
      attcatcac aattgctcc ccctcgtat caagccaac 
      gaagtactt aaagtaaaa aaatacaaa cacggattt 
      attcataat ccggtcgtt ttgtgtccg acaaatacg 
      gttgctgat gtgctcaaa actaagaag gaacaaggt 
      ttttcgggc attccgatc actgaagat ggaaaaatg 
      ggcggaaag cttgttgga attgtgacg tcgagagat 
      cttgatttt cttgaagat caaaattac tcaaactct 
      aaactcgaa actataatg accaaacta gaagatctc 
      gtaacggct cagtctgga gtgaccctt cccgaagcc 
      aactcaatc cttgccaaa agcaaaaag ggcaaactc 
      ccaatcgta aacgccgaa ggcaacctc gttgcctta 
      atggcccga accgatttg aaaaaagcg aaaagctac 
      cccacagca tctaaagac gataataaa caattgatc 
      gtcggagca gctattgga acacgcgac gaagacaaa 
      gaacgactg aaactccta gtcaatgcc ggagttgac 
      gttattgtt ctcgattcc tcgcaagga aattcagtt 
      tatcaaata gaaatgatc aaatatatt aaaaaatcg 
      tatccgagc ctgcaagtg attgctggg aatgtggta 
      accgccaaa caggcgaag aatttaata gatgctggt 
      gccgatgcg ctacgttgt ggcatgggg agtgggagt 
      atttgcata acgcaggag gtgatggcc gttgggagg 
      gcacaagct acagcggtc tacagggtg gcgcagtat 
      gcgaagaga tacggagtg cctgttatt gcagatggg 
      gggattcaa tcgattggg cacataatc aaggctttg 
      gcattgggg gcatcttct gtgatgatg gggtcgatg 
      ctggccggt acctccgaa gcacccggc gaatattac 
      ttttctgat ggtgtaaga ttaaaaaaa tataggggt 
      atgggcagt attgaagca atgaaccgg aaagatgcc 
      atgggttct gccatgaac agatacttc cacagcgat 
      gccgacaaa ctaaaagtg gctcaaggt gttagtgga 
      agtattgta gacaaggga tctgttttc cgcttcgtt 
      ccatatttg caatgtgga attagacac ggctgtcaa 
      gatatagga gtcaaatct ctgactaag cttaaagaa 
      ttgatcgat tgtggggag gttcgattt gagaagaga 
      acacattcg gcgcaattg gaaggaaac gttcatggt 
      ttattctcg tacgagaaa agattgttt tag
    mRNA sequence
    • >TC005099 RA
      gttctttta cctctaaca gcttctgtg attggacgg 
      ttatcgctc caagtccgg aatcctccc ttctgctcg 
      atatttcac cgatataat caatagtag tccaaatgg 
      ctgacagca acagtttgc ttgaagatg gccaatcac 
      tccaagaca tgatgaacg atggcgttg gactcactt 
      acaatgact ttataatcc tcccaggat atatcgatt 
      ttacaccgg acgcagttg acttgacga cccaattga 
      ccaaaaaaa taacgctca aatcgccgc ttgtttcgt 
      ctccgatgg acactgtga cagaatcgt caatggcga 
      ttgcaatgg cgctttgtg gtgggattg gggtgattc 
      atcacaatt gctccccct cgtatcaag ccaacgaag 
      tacttaaag taaaaaaat acaaacacg gatttattc 
      ataatccgg tcgttttgt gtccgacaa atacggttg 
      ctgatgtgc tcaaaacta agaaggaac aaggttttt 
      cgggcattc cgatcactg aagatggaa aaatgggcg 
      gaaagcttg ttggaattg tgacgtcga gagatcttg 
      attttcttg aagatcaaa attactcaa actctaaac 
      tcgaaacta taatgacca aactagaag atctcgtaa 
      cggctcagt ctggagtga cccttcccg aagccaact 
      caatccttg ccaaaagca aaaagggca aactcccaa 
      tcgtaaacg ccgaaggca acctcgttg ccttaatgg 
      cccgaaccg atttgaaaa aagcgaaaa gctacccca 
      cagcatcta aagacgata ataaacaat tgatcgtcg 
      gagcagcta ttggaacac gcgacgaag acaaagaac 
      gactgaaac tcctagtca atgccggag ttgacgtta 
      ttgttctcg attcctcgc aaggaaatt cagtttatc 
      aaatagaaa tgatcaaat atattaaaa aatcgtatc 
      cgagcctgc aagtgattg ctgggaatg tggtaaccg 
      ccaaacagg cgaagaatt taatagatg ctggtgccg 
      atgcgctac gttgtggca tggggagtg ggagtattt 
      gcataacgc aggaggtga tggccgttg ggagggcac 
      aagctacag cggtctaca gggtggcgc agtatgcga 
      agagatacg gagtgcctg ttattgcag atgggggga 
      ttcaatcga ttgggcaca taatcaagg ctttggcat 
      tgggggcat cttctgtga tgatggggt cgatgctgg 
      ccggtacct ccgaagcac ccggcgaat attactttt 
      ctgatggtg taagattaa aaaaatata ggggtatgg 
      gcagtattg aagcaatga accggaaag atgccatgg 
      gttctgcca tgaacagat acttccaca gcgatgccg 
      acaaactaa aagtggctc aaggtgtta gtggaagta 
      ttgtagaca agggatctg ttttccgct tcgttccat 
      atttgcaat gtggaatta gacacggct gtcaagata 
      taggagtca aatctctga ctaagctta aagaattga 
      tcgattgtg gggaggttc gatttgaga agagaacac 
      attcggcgc aattggaag gaaacgttc atggtttat 
      tctcgtacg agaaaagat tgttttagc aagagtgcg 
      gtgggaatt aagttgtaa ataaatact tatgtgata 
      taggtacga tcggtgtcg cattttctg atatttgta 
      aatattgtg tatttccaa caatttgcg tttacactt 
      ttaaacaat catttttgt tgtgaaagt attatggaa 
      attttaata caatagaag catatttat ttaagacta 
      tatatttat aagtgtacc tatattttt tatattagt 
      tatgtttta ttaaaaaat tatgaaaat g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY