Status
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Gene information
General information
TC033493
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005094,TC030417
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 1325730..1347981
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033493
    iBeetle screen iB_00803
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    egg chamber mostly shape rounded - Number of animals showing the phenotype: 4

    egg chamber potentially degenerated - Number of animals showing the phenotype: 4
    egg chamber sometimes fused - Number of animals showing the phenotype: 4
    previtellogenic egg chamber mostly not present - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033493 RA
      MNFDFSEPE KQEEDYLEL LKLCFIDLF RTYFAKFFL 
      TIAFINITA LTILQTYYS LTSFNTVQF LKYAPVYFG 
      RFHAFICLC FILHYIHVT DKLFENVPR WSYTTIGKK 
      YDNEVKRNA LYTNILVAC IAIAVCHKR LKTPVGSIL 
      LHSVTKARS RRASIQNHI VSSKMGKTP AIGIDLGTT 
      YSCVGVWQQ GKVEIIANE QGNRTTPSY VAFTDTERL 
      IGDAAKNQV AMNPSNTVF DAKRLIGRK FDDPKIQQD 
      MKHWPFKVV NDGGKPKIQ VEFKGEVKR FAPEEISSM 
      VLRKMKETA DAFLGKETK DAVITVPAY FNDSQRQAT 
      KDAGMIAGI NVLRIINEP TAAALAYGL DKNLKGERN 
      VLIFDLGGG TFDVSILTI DEGSLFEVK ATAGDTHLG 
      GEDFDNRLV NHFADEFKR KYKKDLTKN PRALRRLRT 
      AAERAKRTL SSSTEASLE IDALFEGID FYTKISRAR 
      FEELNSDLF RGTLEPVEK ALNDAKMDK SMIHDVVLV 
      GGSTRIPKI QNLLQNYFS GKTLNLSIN PDEAVAYGA 
      AVQAAILSG DSSSKIQDV LLVDVTPLS LGIETAGGV 
      MSKIVERNA RIPCKQTQT FTTYSDNQP AVSIQVFEG 
      ERAMTKDNN LLGTFDLSG IPPAPRGVP KIDVTFDLD 
      ANGILNVSA KEVSTGKSK NITIKNDKG RLSQREIEK 
      MLADAERYK EEDDRQRER IGSKNQLES YVFNVKQAV 
      EEAGNKLSA EDKKKALKE CEACIKWLD SNQMAEKEE 
      FEYKLKDLT KICSPIMTK LHRGGGSSG GGGNDGPTI 
      EEVD
    CDS sequence
    • >TC033493 RA
      atgaacttc gattttagc gaacccgaa aagcaagaa 
      gaagattat ttggaacta cttaaactt tgcttcatc 
      gatttgttt cggacctat ttcgccaag ttctttctc 
      accattgcg ttcatcaac attacagca ttgaccatt 
      cttcagacg tactactct ctcaccagt ttcaatacg 
      gtacaattt ttaaaatac gctcctgtt tatttcgga 
      cgctttcac gcattcata tgtttgtgt ttcatcttg 
      cattatata catgttacg gacaagttg tttgaaaat 
      gttccccgg tggagttat accactatt ggaaaaaag 
      tatgataac gaggttaag agaaacgcc ctgtataca 
      aacatcttg gtcgcttgc attgcgata gcagtgtgc 
      cacaaacga cttaaaact cccgttggg tcgattttg 
      cttcactcg gtcacaaaa gctcgaagt aggcgcgcc 
      tcaattcaa aatcacatc gtgtcaagc aaaatgggc 
      aaaacccca gcgattggc atcgacttg ggcacgacg 
      tactcctgt gtgggtgtg tggcagcaa ggcaaagtg 
      gagatcatc gccaacgag cagggaaat cgcaccact 
      cccagctat gtggccttc accgacacg gagcgcctc 
      atcggcgac gcggccaag aaccaagtg gcgatgaat 
      cccagcaac acggtgttc gatgcgaag aggctgatt 
      gggaggaaa ttcgacgat ccgaagatt cagcaggat 
      atgaagcat tggccgttc aaggtggtg aatgacgga 
      gggaagccg aagatccag gtggagttt aaaggggag 
      gttaagagg tttgcgccg gaggagatt agtagtatg 
      gtgttgagg aagatgaag gagacggcc gatgcgttt 
      ttgggaaag gagaccaag gatgcggtt attacggtt 
      ccggcgtat tttaacgac tcgcagagg caggctacc 
      aaggacgct gggatgatc gcaggtatc aacgtttta 
      cgaataatc aacgaacca acagctgca gctctagcc 
      tacggcttg gacaaaaac ctaaaaggc gaacgcaac 
      gtccttatt ttcgattta ggcggtggc acttttgac 
      gtttcaatt ctaacaatc gacgaaggt tcacttttc 
      gaagtcaaa gcaacagcc ggcgataca catttagga 
      ggcgaagac tttgataac cgccttgtt aatcatttc 
      gccgacgaa tttaaacgc aaatacaaa aaagactta 
      accaaaaac ccgcgagct ttgcgccgt cttcgaacg 
      gccgcagaa agagctaag cgaactctg agttctagc 
      acggaagca tctcttgaa attgacgct ctgtttgaa 
      gggattgac ttttatacg aagatttca agagctagg 
      tttgaagaa ctcaactct gatttattc agaggtact 
      ttggaaccg gtcgaaaag gcactgaac gatgccaag 
      atggataag tctatgata catgatgtt gtgctggtt 
      gggggttcg accagaatt cccaaaatt caaaatttg 
      ctccagaat tatttttcg gggaagacc ctaaatttg 
      agtattaat cccgacgaa gctgttgct tatggtgcg 
      gctgttcaa gctgcaatt ttgagtggt gatagcagt 
      tcgaaaata caagacgtg ttgcttgtt gatgttaca 
      cctttatca cttggtatc gaaacagca ggaggtgtt 
      atgtcgaag attgtagaa cgcaatgcg agaattccc 
      tgcaaacaa actcaaact tttaccact tattccgat 
      aatcaacca gctgttagt attcaagtg tttgaaggg 
      gagagagcc atgacaaag gataacaac ctgttgggg 
      acgtttgat ttgtctgga atcccacct gcaccacgt 
      ggcgtccca aaaatcgac gttactttt gatttggac 
      gcaaatgga attctgaat gtttctgca aaggaggtt 
      agtaccggg aagtccaaa aacatcacc atcaagaac 
      gacaaagga agactgtcg caaagagaa atcgagaaa 
      atgttagcc gatgcggaa cgttacaag gaggaagat 
      gacagacaa agggaacga attggaagc aagaatcag 
      ctcgagagt tatgttttt aatgtgaaa caagcggtg 
      gaagaagct gggaacaag ctaagtgcc gaagataag 
      aaaaaagct ttgaaggag tgtgaggct tgtattaaa 
      tggttggat agtaaccaa atggcggaa aaagaagag 
      tttgagtat aagttgaaa gatttgacg aaaatttgc 
      agtccgatt atgaccaaa ttacataga ggtggtggt 
      tcaagtggt ggtggggga aatgatggc ccaacaatt 
      gaagaagtg gattaa
    mRNA sequence
    • >TC033493 RA
      ttgaagcaa gtcgctaaa atgaacttc gattttagc 
      gaacccgaa aagcaagaa gaagattat ttggaacta 
      cttaaactt tgcttcatc gatttgttt cggacctat 
      ttcgccaag ttctttctc accattgcg ttcatcaac 
      attacagca ttgaccatt cttcagacg tactactct 
      ctcaccagt ttcaatacg gtacaattt ttaaaatac 
      gctcctgtt tatttcgga cgctttcac gcattcata 
      tgtttgtgt ttcatcttg cattatata catgttacg 
      gacaagttg tttgaaaat gttccccgg tggagttat 
      accactatt ggaaaaaag tatgataac gaggttaag 
      agaaacgcc ctgtataca aacatcttg gtcgcttgc 
      attgcgata gcagtgtgc cacaaacga cttaaaact 
      cccgttggg tcgattttg cttcactcg gtcacaaaa 
      gctcgaagt aggcgcgcc tcaattcaa aatcacatc 
      gtgtcaagc aaaatgggc aaaacccca gcgattggc 
      atcgacttg ggcacgacg tactcctgt gtgggtgtg 
      tggcagcaa ggcaaagtg gagatcatc gccaacgag 
      cagggaaat cgcaccact cccagctat gtggccttc 
      accgacacg gagcgcctc atcggcgac gcggccaag 
      aaccaagtg gcgatgaat cccagcaac acggtgttc 
      gatgcgaag aggctgatt gggaggaaa ttcgacgat 
      ccgaagatt cagcaggat atgaagcat tggccgttc 
      aaggtggtg aatgacgga gggaagccg aagatccag 
      gtggagttt aaaggggag gttaagagg tttgcgccg 
      gaggagatt agtagtatg gtgttgagg aagatgaag 
      gagacggcc gatgcgttt ttgggaaag gagaccaag 
      gatgcggtt attacggtt ccggcgtat tttaacgac 
      tcgcagagg caggctacc aaggacgct gggatgatc 
      gcaggtatc aacgtttta cgaataatc aacgaacca 
      acagctgca gctctagcc tacggcttg gacaaaaac 
      ctaaaaggc gaacgcaac gtccttatt ttcgattta 
      ggcggtggc acttttgac gtttcaatt ctaacaatc 
      gacgaaggt tcacttttc gaagtcaaa gcaacagcc 
      ggcgataca catttagga ggcgaagac tttgataac 
      cgccttgtt aatcatttc gccgacgaa tttaaacgc 
      aaatacaaa aaagactta accaaaaac ccgcgagct 
      ttgcgccgt cttcgaacg gccgcagaa agagctaag 
      cgaactctg agttctagc acggaagca tctcttgaa 
      attgacgct ctgtttgaa gggattgac ttttatacg 
      aagatttca agagctagg tttgaagaa ctcaactct 
      gatttattc agaggtact ttggaaccg gtcgaaaag 
      gcactgaac gatgccaag atggataag tctatgata 
      catgatgtt gtgctggtt gggggttcg accagaatt 
      cccaaaatt caaaatttg ctccagaat tatttttcg 
      gggaagacc ctaaatttg agtattaat cccgacgaa 
      gctgttgct tatggtgcg gctgttcaa gctgcaatt 
      ttgagtggt gatagcagt tcgaaaata caagacgtg 
      ttgcttgtt gatgttaca cctttatca cttggtatc 
      gaaacagca ggaggtgtt atgtcgaag attgtagaa 
      cgcaatgcg agaattccc tgcaaacaa actcaaact 
      tttaccact tattccgat aatcaacca gctgttagt 
      attcaagtg tttgaaggg gagagagcc atgacaaag 
      gataacaac ctgttgggg acgtttgat ttgtctgga 
      atcccacct gcaccacgt ggcgtccca aaaatcgac 
      gttactttt gatttggac gcaaatgga attctgaat 
      gtttctgca aaggaggtt agtaccggg aagtccaaa 
      aacatcacc atcaagaac gacaaagga agactgtcg 
      caaagagaa atcgagaaa atgttagcc gatgcggaa 
      cgttacaag gaggaagat gacagacaa agggaacga 
      attggaagc aagaatcag ctcgagagt tatgttttt 
      aatgtgaaa caagcggtg gaagaagct gggaacaag 
      ctaagtgcc gaagataag aaaaaagct ttgaaggag 
      tgtgaggct tgtattaaa tggttggat agtaaccaa 
      atggcggaa aaagaagag tttgagtat aagttgaaa 
      gatttgacg aaaatttgc agtccgatt atgaccaaa 
      ttacataga ggtggtggt tcaagtggt ggtggggga 
      aatgatggc ccaacaatt gaagaagtg gattaattt 
      aataacttt gtctcatca tagcttgga tttttggct 
      tgcttagtt ggaaatttg tgtgataat ctagatttg 
      aaataaaga gctttattc tac