Status
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Gene information
General information
TC033493
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005094,TC030417 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 1631152..1653403
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033493
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00803

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Fertility
    egg chamber mostly shape rounded - Number of animals showing the phenotype: 4

    egg chamber potentially degenerated - Number of animals showing the phenotype: 4
    egg chamber sometimes fused - Number of animals showing the phenotype: 4
    previtellogenic egg chamber mostly not present - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033493 RA
      MNFDFSEPE KQEEDYLEL LKLCFIDLF RTYFAKFFL 
TIAFINITA LTILQTYYS LTSFNTVQF LKYAPVYFG 
RFHAFICLC FILHYIHVT DKLFENVPR WSYTTIGKK 
YDNEVKRNA LYTNILVAC IAIAVCHKR LKTPVGSIL 
LHSVTKARS RRASIQNHI VSSKMGKTP AIGIDLGTT 
YSCVGVWQQ GKVEIIANE QGNRTTPSY VAFTDTERL 
IGDAAKNQV AMNPSNTVF DAKRLIGRK FDDPKIQQD 
MKHWPFKVV NDGGKPKIQ VEFKGEVKR FAPEEISSM 
VLRKMKETA DAFLGKETK DAVITVPAY FNDSQRQAT 
KDAGMIAGI NVLRIINEP TAAALAYGL DKNLKGERN 
VLIFDLGGG TFDVSILTI DEGSLFEVK ATAGDTHLG 
GEDFDNRLV NHFADEFKR KYKKDLTKN PRALRRLRT 
AAERAKRTL SSSTEASLE IDALFEGID FYTKISRAR 
FEELNSDLF RGTLEPVEK ALNDAKMDK SMIHDVVLV 
GGSTRIPKI QNLLQNYFS GKTLNLSIN PDEAVAYGA 
AVQAAILSG DSSSKIQDV LLVDVTPLS LGIETAGGV 
MSKIVERNA RIPCKQTQT FTTYSDNQP AVSIQVFEG 
ERAMTKDNN LLGTFDLSG IPPAPRGVP KIDVTFDLD 
ANGILNVSA KEVSTGKSK NITIKNDKG RLSQREIEK 
MLADAERYK EEDDRQRER IGSKNQLES YVFNVKQAV 
EEAGNKLSA EDKKKALKE CEACIKWLD SNQMAEKEE 
FEYKLKDLT KICSPIMTK LHRGGGSSG GGGNDGPTI 
EEVD
    CDS sequence
    • >TC033493 RA
      atgaacttc gattttagc gaacccgaa aagcaagaa 
gaagattat ttggaacta cttaaactt tgcttcatc 
gatttgttt cggacctat ttcgccaag ttctttctc 
accattgcg ttcatcaac attacagca ttgaccatt 
cttcagacg tactactct ctcaccagt ttcaatacg 
gtacaattt ttaaaatac gctcctgtt tatttcgga 
cgctttcac gcattcata tgtttgtgt ttcatcttg 
cattatata catgttacg gacaagttg tttgaaaat 
gttccccgg tggagttat accactatt ggaaaaaag 
tatgataac gaggttaag agaaacgcc ctgtataca 
aacatcttg gtcgcttgc attgcgata gcagtgtgc 
cacaaacga cttaaaact cccgttggg tcgattttg 
cttcactcg gtcacaaaa gctcgaagt aggcgcgcc 
tcaattcaa aatcacatc gtgtcaagc aaaatgggc 
aaaacccca gcgattggc atcgacttg ggcacgacg 
tactcctgt gtgggtgtg tggcagcaa ggcaaagtg 
gagatcatc gccaacgag cagggaaat cgcaccact 
cccagctat gtggccttc accgacacg gagcgcctc 
atcggcgac gcggccaag aaccaagtg gcgatgaat 
cccagcaac acggtgttc gatgcgaag aggctgatt 
gggaggaaa ttcgacgat ccgaagatt cagcaggat 
atgaagcat tggccgttc aaggtggtg aatgacgga 
gggaagccg aagatccag gtggagttt aaaggggag 
gttaagagg tttgcgccg gaggagatt agtagtatg 
gtgttgagg aagatgaag gagacggcc gatgcgttt 
ttgggaaag gagaccaag gatgcggtt attacggtt 
ccggcgtat tttaacgac tcgcagagg caggctacc 
aaggacgct gggatgatc gcaggtatc aacgtttta 
cgaataatc aacgaacca acagctgca gctctagcc 
tacggcttg gacaaaaac ctaaaaggc gaacgcaac 
gtccttatt ttcgattta ggcggtggc acttttgac 
gtttcaatt ctaacaatc gacgaaggt tcacttttc 
gaagtcaaa gcaacagcc ggcgataca catttagga 
ggcgaagac tttgataac cgccttgtt aatcatttc 
gccgacgaa tttaaacgc aaatacaaa aaagactta 
accaaaaac ccgcgagct ttgcgccgt cttcgaacg 
gccgcagaa agagctaag cgaactctg agttctagc 
acggaagca tctcttgaa attgacgct ctgtttgaa 
gggattgac ttttatacg aagatttca agagctagg 
tttgaagaa ctcaactct gatttattc agaggtact 
ttggaaccg gtcgaaaag gcactgaac gatgccaag 
atggataag tctatgata catgatgtt gtgctggtt 
gggggttcg accagaatt cccaaaatt caaaatttg 
ctccagaat tatttttcg gggaagacc ctaaatttg 
agtattaat cccgacgaa gctgttgct tatggtgcg 
gctgttcaa gctgcaatt ttgagtggt gatagcagt 
tcgaaaata caagacgtg ttgcttgtt gatgttaca 
cctttatca cttggtatc gaaacagca ggaggtgtt 
atgtcgaag attgtagaa cgcaatgcg agaattccc 
tgcaaacaa actcaaact tttaccact tattccgat 
aatcaacca gctgttagt attcaagtg tttgaaggg 
gagagagcc atgacaaag gataacaac ctgttgggg 
acgtttgat ttgtctgga atcccacct gcaccacgt 
ggcgtccca aaaatcgac gttactttt gatttggac 
gcaaatgga attctgaat gtttctgca aaggaggtt 
agtaccggg aagtccaaa aacatcacc atcaagaac 
gacaaagga agactgtcg caaagagaa atcgagaaa 
atgttagcc gatgcggaa cgttacaag gaggaagat 
gacagacaa agggaacga attggaagc aagaatcag 
ctcgagagt tatgttttt aatgtgaaa caagcggtg 
gaagaagct gggaacaag ctaagtgcc gaagataag 
aaaaaagct ttgaaggag tgtgaggct tgtattaaa 
tggttggat agtaaccaa atggcggaa aaagaagag 
tttgagtat aagttgaaa gatttgacg aaaatttgc 
agtccgatt atgaccaaa ttacataga ggtggtggt 
tcaagtggt ggtggggga aatgatggc ccaacaatt 
gaagaagtg gattaa
    mRNA sequence
    • >TC033493 RA
      ttgaagcaa gtcgctaaa atgaacttc gattttagc 
gaacccgaa aagcaagaa gaagattat ttggaacta 
cttaaactt tgcttcatc gatttgttt cggacctat 
ttcgccaag ttctttctc accattgcg ttcatcaac 
attacagca ttgaccatt cttcagacg tactactct 
ctcaccagt ttcaatacg gtacaattt ttaaaatac 
gctcctgtt tatttcgga cgctttcac gcattcata 
tgtttgtgt ttcatcttg cattatata catgttacg 
gacaagttg tttgaaaat gttccccgg tggagttat 
accactatt ggaaaaaag tatgataac gaggttaag 
agaaacgcc ctgtataca aacatcttg gtcgcttgc 
attgcgata gcagtgtgc cacaaacga cttaaaact 
cccgttggg tcgattttg cttcactcg gtcacaaaa 
gctcgaagt aggcgcgcc tcaattcaa aatcacatc 
gtgtcaagc aaaatgggc aaaacccca gcgattggc 
atcgacttg ggcacgacg tactcctgt gtgggtgtg 
tggcagcaa ggcaaagtg gagatcatc gccaacgag 
cagggaaat cgcaccact cccagctat gtggccttc 
accgacacg gagcgcctc atcggcgac gcggccaag 
aaccaagtg gcgatgaat cccagcaac acggtgttc 
gatgcgaag aggctgatt gggaggaaa ttcgacgat 
ccgaagatt cagcaggat atgaagcat tggccgttc 
aaggtggtg aatgacgga gggaagccg aagatccag 
gtggagttt aaaggggag gttaagagg tttgcgccg 
gaggagatt agtagtatg gtgttgagg aagatgaag 
gagacggcc gatgcgttt ttgggaaag gagaccaag 
gatgcggtt attacggtt ccggcgtat tttaacgac 
tcgcagagg caggctacc aaggacgct gggatgatc 
gcaggtatc aacgtttta cgaataatc aacgaacca 
acagctgca gctctagcc tacggcttg gacaaaaac 
ctaaaaggc gaacgcaac gtccttatt ttcgattta 
ggcggtggc acttttgac gtttcaatt ctaacaatc 
gacgaaggt tcacttttc gaagtcaaa gcaacagcc 
ggcgataca catttagga ggcgaagac tttgataac 
cgccttgtt aatcatttc gccgacgaa tttaaacgc 
aaatacaaa aaagactta accaaaaac ccgcgagct 
ttgcgccgt cttcgaacg gccgcagaa agagctaag 
cgaactctg agttctagc acggaagca tctcttgaa 
attgacgct ctgtttgaa gggattgac ttttatacg 
aagatttca agagctagg tttgaagaa ctcaactct 
gatttattc agaggtact ttggaaccg gtcgaaaag 
gcactgaac gatgccaag atggataag tctatgata 
catgatgtt gtgctggtt gggggttcg accagaatt 
cccaaaatt caaaatttg ctccagaat tatttttcg 
gggaagacc ctaaatttg agtattaat cccgacgaa 
gctgttgct tatggtgcg gctgttcaa gctgcaatt 
ttgagtggt gatagcagt tcgaaaata caagacgtg 
ttgcttgtt gatgttaca cctttatca cttggtatc 
gaaacagca ggaggtgtt atgtcgaag attgtagaa 
cgcaatgcg agaattccc tgcaaacaa actcaaact 
tttaccact tattccgat aatcaacca gctgttagt 
attcaagtg tttgaaggg gagagagcc atgacaaag 
gataacaac ctgttgggg acgtttgat ttgtctgga 
atcccacct gcaccacgt ggcgtccca aaaatcgac 
gttactttt gatttggac gcaaatgga attctgaat 
gtttctgca aaggaggtt agtaccggg aagtccaaa 
aacatcacc atcaagaac gacaaagga agactgtcg 
caaagagaa atcgagaaa atgttagcc gatgcggaa 
cgttacaag gaggaagat gacagacaa agggaacga 
attggaagc aagaatcag ctcgagagt tatgttttt 
aatgtgaaa caagcggtg gaagaagct gggaacaag 
ctaagtgcc gaagataag aaaaaagct ttgaaggag 
tgtgaggct tgtattaaa tggttggat agtaaccaa 
atggcggaa aaagaagag tttgagtat aagttgaaa 
gatttgacg aaaatttgc agtccgatt atgaccaaa 
ttacataga ggtggtggt tcaagtggt ggtggggga 
aatgatggc ccaacaatt gaagaagtg gattaattt 
aataacttt gtctcatca tagcttgga tttttggct 
tgcttagtt ggaaatttg tgtgataat ctagatttg 
aaataaaga gctttattc tac

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY