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Gene information
General information
TC005074 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7193829..7199492
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005074
    iBeetle screen iB_00800

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005074 RA
      MPRPFLRTE SPRGYRAAM SETTPDMGI VKSELVTSP 
      HLLDHGYGA TPQNQLLPS AAPNATAPV KRRLNLELE 
      RGRVVPLKQ EFKPPQPRK QKRLTPTKK NTRYDTSLG 
      LLTQKFSAL LEESPNGVV DLNKASQQL NVQKRRIYD 
      ITNVLEGIG IIEKKSKNN IQWKASRKD NEKFLKLTK 
      DLQDLENQE NDLNRMINT VAKQLIGLN NDKHGFVTY 
      QDLRSIEKF KQNTVIVVK APPKTHLSV KTASKEDDK 
      YSIQLKSDT GEIEVFLCP EYVPPAKPK TEPLEDDFK 
      DIKFSPSFD LTTPPVPLL DSPKAPHKQ ISSQICRNL 
      SFTKDPVVS ARTPLSVQN PFTYNNVLT SSQEPAPGE 
      VDPLRLSPY ASLVDGYPT CLDSSSLKM GTLPLINDN 
      EVLGPMMGP YSMHTGNIQ TGYTVDSFF SNEPFLHLE 
      PPAMHSEYN FSLDANEGL RDLFDFL
    CDS sequence
    • >TC005074 RA
      atgcctcgt cctttcctc cgcacggaa agccccagg 
      gggtacaga gctgccatg tccgagaca acccccgac 
      atgggcatc gtcaagtcc gagctggtg accagcccc 
      cacttgctg gaccacggc tatggggcc acgccgcaa 
      aaccagttg ctcccgtcg gcggctccg aacgcaact 
      gcgcccgtt aaacgcaga cttaacctc gaactcgag 
      cgggggaga gtggtgcct ctgaagcag gagttcaag 
      ccgccgcag ccccggaag caaaaacgg ctcaccccc 
      acgaagaaa aacacgcgt tatgacacg tccttgggg 
      ctcctcacg cagaagttc tcggcactg cttgaggaa 
      tcccccaat ggggttgtc gatctgaac aaggcttcg 
      cagcagctc aacgtgcaa aagcgccgg atttacgac 
      atcacgaac gtgttggag ggcattggc attattgag 
      aaaaagtcc aagaacaac atccagtgg aaagccagt 
      cggaaagac aacgaaaag ttcctcaaa ctcaccaag 
      gacctgcag gacctggag aaccaggaa aacgacctc 
      aaccgaatg atcaacacg gtggctaag cagctgatc 
      gggttgaac aacgacaag cacgggttt gtcacgtat 
      caggacctg aggtcgata gagaagttc aagcagaac 
      acggtcatc gtggtcaaa gcgcccccg aaaacgcat 
      ttatcggtc aaaaccgcg tccaaagag gacgataaa 
      tacagtatt caattgaag agcgacacg ggggagatc 
      gaggtgttc ctctgtcct gagtacgtc ccacctgcc 
      aaaccaaag actgaaccg ctcgaagac gactttaaa 
      gatatcaaa ttcagtcca agtttcgat ttgactact 
      ccacctgtg cctcttctc gacagtcct aaagcaccg 
      cacaagcag atttcctcg cagatatgc aggaactta 
      tcatttacg aaagatccc gtggtcagt gcccgtaca 
      ccattatca gtacaaaat ccgtttaca tataataac 
      gttttaacg tcgtcgcaa gaaccagcg cctggagaa 
      gtggacccg ttacgactt tccccttac gcgtcattg 
      gtggatggt tacccaact tgtttggat tccagcagt 
      ttgaaaatg gggacgcta cccctcatt aatgataat 
      gaggtactc gggcccatg atgggacct tatagcatg 
      cacacagga aacatacaa acagggtat acagttgac 
      tcatttttc tcaaacgag ccgtttcta catttggaa 
      ccgccagca atgcactct gagtataac ttcagtttg 
      gatgcaaac gaagggttg cgagatctc tttgatttc 
      ttataa
    mRNA sequence
    • >TC005074 RA
      cccggtaca gatggcaga tgttttgta accagtgcg 
      tacttgtac acgcttggc aaaatcgta agcaatttc 
      aataatttt ttgtgagcc gcctagccc ccctatcgc 
      aaatccaca aacgccgcg tgaaagccc acactttgc 
      ggaacatga atctggtaa aattttcgt tattagccg 
      aggcccgat gcagttgag gccgagttt gtcgccgcg 
      aacccagct gctttgtat aaagtggtt tggaaacca 
      ttttgttta gtattccgg aacatctcg tttttgagc 
      cggtgtttt cggaaatcc ctcccttga gccagtaag 
      acagtgcaa gacaccctc gcaaggagg cccgagctg 
      ccagatcgg ccaccacgt caattttaa tgatttgat 
      ttgctataa tgcctcgtc ctttcctcc gcacggaaa 
      gccccaggg ggtacagag ctgccatgt ccgagacaa 
      cccccgaca tgggcatcg tcaagtccg agctggtga 
      ccagccccc acttgctgg accacggct atggggcca 
      cgccgcaaa accagttgc tcccgtcgg cggctccga 
      acgcaactg cgcccgtta aacgcagac ttaacctcg 
      aactcgagc gggggagag tggtgcctc tgaagcagg 
      agttcaagc cgccgcagc cccggaagc aaaaacggc 
      tcaccccca cgaagaaaa acacgcgtt atgacacgt 
      ccttggggc tcctcacgc agaagttct cggcactgc 
      ttgaggaat cccccaatg gggttgtcg atctgaaca 
      aggcttcgc agcagctca acgtgcaaa agcgccgga 
      tttacgaca tcacgaacg tgttggagg gcattggca 
      ttattgaga aaaagtcca agaacaaca tccagtgga 
      aagccagtc ggaaagaca acgaaaagt tcctcaaac 
      tcaccaagg acctgcagg acctggaga accaggaaa 
      acgacctca accgaatga tcaacacgg tggctaagc 
      agctgatcg ggttgaaca acgacaagc acgggtttg 
      tcacgtatc aggacctga ggtcgatag agaagttca 
      agcagaaca cggtcatcg tggtcaaag cgcccccga 
      aaacgcatt tatcggtca aaaccgcgt ccaaagagg 
      acgataaat acagtattc aattgaaga gcgacacgg 
      gggagatcg aggtgttcc tctgtcctg agtacgtcc 
      cacctgcca aaccaaaga ctgaaccgc tcgaagacg 
      actttaaag atatcaaat tcagtccaa gtttcgatt 
      tgactactc cacctgtgc ctcttctcg acagtccta 
      aagcaccgc acaagcaga tttcctcgc agatatgca 
      ggaacttat catttacga aagatcccg tggtcagtg 
      cccgtacac cattatcag tacaaaatc cgtttacat 
      ataataacg ttttaacgt cgtcgcaag aaccagcgc 
      ctggagaag tggacccgt tacgacttt ccccttacg 
      cgtcattgg tggatggtt acccaactt gtttggatt 
      ccagcagtt tgaaaatgg ggacgctac ccctcatta 
      atgataatg aggtactcg ggcccatga tgggacctt 
      atagcatgc acacaggaa acatacaaa cagggtata 
      cagttgact catttttct caaacgagc cgtttctac 
      atttggaac cgccagcaa tgcactctg agtataact 
      tcagtttgg atgcaaacg aagggttgc gagatctct 
      ttgatttct tataaagga aatcagttt tcatcattg 
      ctcatgtac aaattatct gcttatgat cacattgat 
      tgtaactgt atctggggt tctcaagat tactaaata 
      gttttcgta cttaattga ctattggaa gctggattt 
      gtacataat cgtattaaa agatgtata ttttactgc 
      ccgtgtgtt tatttatgg cacaaattt gtaaatatc 
      gcagtgagt tttggaagt tgtaatgaa acggctcaa 
      accacattc ctaattcga tgttttctc tggactatt 
      tcgtaatga tgagaacct tgtatttgt gtcatttca 
      ttcatttta tatttattg ttgagttaa cattggtta 
      aatattaaa attagagtt ttacaaata ttttgtaag 
      gagaaactg gaccaaagt gcctattgt ttggtcacg 
      attaatatt taatttttg agcagtctc agtgtcaaa 
      cttatttat tcaaaccga cagaactta tcactacta 
      tcaaaagga gttaatatg aaatggaat caaatggtg 
      ccattggaa aatctcttg taaatatat ttatacacg 
      cttatgggg catttttgt ctgttttaa ttccataat 
      tagaagttt aagagaaaa ttatataga ttttacctg 
      ctgttgttt tctttttgt tagtaaata cattgtttg 
      cacccactt ctggatcac cttgctctt gttgttttt 
      aataaaatg ataattgcg ctattgttg caataagtc 
      cgtactagt ccttttaca gttgcaatt tcgtaagat 
      ttgtaattt tttgaacaa tattttaaa tcttgtttg 
      actgagtat ttagtattg caatttttg tagatgaag 
      gtgggtgtt cttgtttat tcagtacaa ttatttttt 
      aattctaat tgttacttc ttctgtaaa taggttatt 
      ttaatgttt atcttttat attattata tattataaa 
      tgtccgaag ccaatagag gccttaaat cagttcggt 
      agaaacatc acatacagg tgatcttta tctgatgcc 
      ctctttgac ttgcctcgg aaccagcga cttcaattt 
      tattgttta ggaccgttc aagctttac ttgtaaaat 
      attctatgc atatcactc caattttta tcgtttgta 
      caacttagc ttgaaatac cgagtcaca tttgccatc 
      ttatcattt cagtttcag ttttctaat tggtcgaaa 
      attgtaata accatgcgg aaaactgaa gatctttgc 
      catgcaaga actgcaaat catatcgaa tgtattttt 
      tattcgaag gtgtggcac gttttggct gcccagttt 
      taaattttt atagctttt tcgagttag aagttatga 
      ggcgttaat aaaaatcat tctgtacat ttttataat 
      gtacataaa attgtaaat taaataata gatgcactt 
      acgttttgt attattaat tttccaatg tccagaatt 
      taatttgac caacttcaa gaaataaaa attgtagaa 
      caag
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