Status
Please wait ...
Gene information
General information
TC005012 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid4: 340868..350451
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005012
    iBeetle screen iB_00796
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    head size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs segment shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs segment broader - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    thorax size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 3

    wings not closed - Number of animals showing the phenotype: 3

    wings shortened - Number of animals showing the phenotype: 3
    legs orientation irregular - Number of animals showing the phenotype: 3
    female genital lobes broader - Number of animals showing the phenotype: 3

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005012 RA
      MGNNAATAN KKVDSAESV KEFLDQAKE DFEEKWKKN 
      PTNTACLDD FERIKTLGT GSFGRVMIV QHKPTKEYY 
      AMKILDKQK VVKLKQVEH TLNEKRILQ AISFPFLVS 
      LRFHFKDNS NLYMVLEYV PGGEMFSHL RKVGRFSEP 
      HSRFYAAQI VLAFEYLHY LDLIYRDLK PENLLIDSQ 
      GYLKVTDFG FAKRVKGRT WTLCGTPEY LAPEIILSK 
      GYNKAVDWW ALGVLVYEM AAGYPPFFA DQPIQIYEK 
      IVSGKVRFP SHFGSDLKD LLRNLLQVD LTKRYGNLK 
      AGVNDIKGH KWFASTDWI AIFQKKIEA PFIPRCKGP 
      GDTSNFDDY EEEALRISS TEKCAKEFA EF
    CDS sequence
    • >TC005012 RA
      atgggcaac aatgccgca acggcgaac aaaaaggtc 
      gactccgcc gaaagcgtg aaggagttc ctcgaccag 
      gccaaggag gacttcgag gagaagtgg aagaagaat 
      ccgacaaac accgcctgt ttggacgac ttcgagcgc 
      attaaaacc ctaggtacg ggttcattc ggacgtgta 
      atgatcgtc caacacaaa cccacgaaa gagtactat 
      gcgatgaaa atcctcgat aagcaaaag gtggtcaag 
      ttgaagcag gtggagcac accctcaac gagaagcgc 
      atcctgcag gcgatcagt ttcccgttc ctagtcagt 
      ctgcggttt cacttcaaa gacaattcg aacttgtac 
      atggtgttg gagtatgtg cctggagga gagatgttt 
      tcgcatttg aggaaagtc ggacgcttc tctgagcca 
      cattcgagg ttttatgct gcccaaatt gtgctcgct 
      ttcgaatac ttgcactat ctagatctg atctacagg 
      gatctgaaa ccggagaat cttctcatc gactcgcag 
      ggatacctc aaggtcacc gatttcggg ttcgctaaa 
      cgcgtcaaa ggtcgcacc tggacctta tgtggtacc 
      cccgaatat ctcgcaccg gagatcatc ctcagcaag 
      ggttacaac aaggcggtg gactggtgg gcactaggt 
      gtcctagtc tatgaaatg gcggctgga tatcctcca 
      tttttcgcc gaccaaccc attcaaatc tacgagaaa 
      atcgtttcg gggaaagtg cgttttccc tcacatttc 
      ggttccgac ctgaaggat ctcctccgt aacctactc 
      caggtggat ttgacaaaa cgttacggt aatttgaaa 
      gccggcgtt aacgacatc aaaggtcac aagtggttt 
      gcgtccact gattggatc gcgattttt caaaagaag 
      attgaagca cctttcata cccaggtgc aagggacct 
      ggagatacg agtaatttc gacgattac gaggaggag 
      gcgttgcgt atctcttcg acggagaag tgcgccaag 
      gaatttgcg gagttttga
    mRNA sequence
    • >TC005012 RA
      attaaaccg tgtaattga cttgcgagc gagtgtctt 
      cgcttcaat ccccaaatg aagtgaaaa tctgggcac 
      ttggtagcc attttatcg ttagctcgt tggtttttc 
      ggcgtgtat tttgaattt gtgttgccc gttttaatc 
      agtgtctgt gcctcaatc gtgcgagat aaactcaat 
      aatgcgtgt gtacggatc gttgtgttg acacaaacc 
      aaacgcgac atccgctcc gagctcatc cttggtgct 
      tcaggcggt gttagaaat caatcgaag ccctggaag 
      gtgtccaag tccaaacca tgggcaaca atgccgcaa 
      cggcgaaca aaaaggtcg actccgccg aaagcgtga 
      aggagttcc tcgaccagg ccaaggagg acttcgagg 
      agaagtgga agaagaatc cgacaaaca ccgcctgtt 
      tggacgact tcgagcgca ttaaaaccc taggtacgg 
      gttcattcg gacgtgtaa tgatcgtcc aacacaaac 
      ccacgaaag agtactatg cgatgaaaa tcctcgata 
      agcaaaagg tggtcaagt tgaagcagg tggagcaca 
      ccctcaacg agaagcgca tcctgcagg cgatcagtt 
      tcccgttcc tagtcagtc tgcggtttc acttcaaag 
      acaattcga acttgtaca tggtgttgg agtatgtgc 
      ctggaggag agatgtttt cgcatttga ggaaagtcg 
      gacgcttct ctgagccac attcgaggt tttatgctg 
      cccaaattg tgctcgctt tcgaatact tgcactatc 
      tagatctga tctacaggg atctgaaac cggagaatc 
      ttctcatcg actcgcagg gatacctca aggtcaccg 
      atttcgggt tcgctaaac gcgtcaaag gtcgcacct 
      ggaccttat gtggtaccc ccgaatatc tcgcaccgg 
      agatcatcc tcagcaagg gttacaaca aggcggtgg 
      actggtggg cactaggtg tcctagtct atgaaatgg 
      cggctggat atcctccat ttttcgccg accaaccca 
      ttcaaatct acgagaaaa tcgtttcgg ggaaagtgc 
      gttttccct cacatttcg gttccgacc tgaaggatc 
      tcctccgta acctactcc aggtggatt tgacaaaac 
      gttacggta atttgaaag ccggcgtta acgacatca 
      aaggtcaca agtggtttg cgtccactg attggatcg 
      cgatttttc aaaagaaga ttgaagcac ctttcatac 
      ccaggtgca agggacctg gagatacga gtaatttcg 
      acgattacg aggaggagg cgttgcgta tctcttcga 
      cggagaagt gcgccaagg aatttgcgg agttttgaa 
      tttttttat gtgtaagta gccttgcca tttcctcag 
      gctattgac ggacttcca gccgagggt ttttgtaag 
      attggtgat tctgcacct aattgtaca gggtgagtc 
      atttatacg cattcactt gggtatggt agtttttgc 
      caaataaaa ataattcca gtga