General information | |||
TC004992 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 557126..574218 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC004992 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00794 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult nutritional state - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (16 dpi)
Comment: at this timepoint still living |
eclosion not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (19 dpi)
wings shortened - Number of animals showing the phenotype: 9
oblique dorsal thoracic muscles fragmented - Number of animals showing the phenotype: 8
female genital lobes decreased - Number of animals showing the phenotype: 9
Comment: black border around the eye is missing. surface of the eye appears plain. the GFP expression of the ommatidia points to a reduced number of ommatidia as well as a malformation of the ommatidia |
antenna shortened - Number of animals showing the phenotype: 6
- >TC004992 RA
MATKRNLPP PNSSGKRFK NDKIFRPPV GDLNPTDDA LIFQELELD HYISAPYPG MPGAQTGTV PVIRMFGIT KVGNSVCCH IHGFSPYFY INLPNDFTQ ADLSRFKEK LNEAVVSDM RSNRDNITE AVLMVELVR GKSLVEYQG EDDNNFAKI TLALPKFIA ASKRLLSTT VVYQPLAHH TFHFFEANV DIETRFMVD TNILGCCWI ELPAGKWFK RPENKFPVT SRCQIEVDI SWTDFISHS PEGEWSPVA RFRIHSFDI ECAGRKGIF PEPNVDPII QIANVIKLH GDEDVVTRN IFTLNSCAP IGHAEVRSF DTEAEMLEE WAEFVRETD PDIFTGYNI NNFDFPYLI ERANHLKLK RFDYLGRIL DIRSSIKET VTQTKIGKR TFKSINFEG RVAYDMLMV IKRDFKLRS YTLNSVSQE ILGEQKEDV HYSVITDLQ NGDEQTRRR LAVYCLKDA DLPVRILST VRSFTNDVE MARVTGVPI TSLLTKGEQ VKVVAQLLK HARAAGYFM PTHQGAPSS EQYEGATVI EPRRGYYTD PIATLDFSS LYPSIMIAH NLCYTTLIK PSIKEKLCL SDEQVTTTP ANCMFVKSS VRPGLLPHI LQHLLAARK KTKAELKEA TDPVVRAVL DGRQLAYKI SANSVYGFT GAQVGKLPC LEISSSVTA YGRTMIEQT KQEVEQHYR IENGYESDA MVIYGDTDS VMVNFRVKS LERSMELGR EAADLISAK FIKPIKLEF EKVYWPYLL INKKRYAGL YFTKPDKYD KMDCKGIET VRRDNCTLV VDVINSCLR KLLIDKDPD GAVNYAKKV ISDLLQNNV DISQLVITK ELTKNDYAA KQAHVELSK KMTKRDPGN APKLGDRIP YVIIAGTKN SKAFEKAED PIYVLENNI PIDSQYYLQ NQLSKPLIR IFQPILGDS AESILLRGE HTRTKTMVT SRVGALSAF TKKKETCLG CRSVLPAGY EAEAVCSHC KPKEATLYQ QELGHHRML ENRFSELFT ECQRCQGSL HEEILCTSR DCPIFYIRK KVQMELDAS KGKISRFGD PIMEEW
- >TC004992 RA
atggcaaca aaacgcaat cttccgcct ccgaattca tcagggaag aggttcaag aacgacaaa atttttcgc cccccagtt ggcgattta aaccccact gatgatgca ctaattttt caagaatta gagttggat cattacatt tcagcccct tatcctgga atgccaggg gctcaaact ggtactgtt cctgtgatt cgaatgttt ggtattaca aaagtgggc aatagtgtg tgttgtcat attcatggt ttttctcca tacttttac attaatctg ccgaatgat tttacacaa gcggatttg tcccgtttc aaggaaaag ttaaatgaa gctgtggta agcgatatg cgctcaaac cgtgacaac ataaccgaa gcagtttta atggtggaa ctggttaga ggaaaatct ctcgtagag tatcaagga gaggacgac aacaacttt gctaaaata accctagcc ttaccgaaa tttattgct gcatctaaa cgtctccta agcactaca gtcgtctat caaccgctt gcccatcac acgtttcat ttctttgaa gcaaatgtg gatattgaa acgcgtttt atggttgat acgaatatt ttgggttgt tgctggatt gagttgcca gcaggcaaa tggttcaaa cgaccagaa aataagttc cctgtaact tcacgttgc caaatagaa gtggacatc tcatggact gactttatt tcacattca ccagaaggt gaatggtca ccagtggct cgatttcgt attcatagt ttcgatatt gaatgtgct ggaagaaaa gggattttt ccggagccg aacgttgat cccattatt caaattgcc aacgtgatt aagttacat ggtgacgag gatgtggtt actcgaaat attttcact ttaaacagt tgcgcaccc ataggacat gctgaggtt cgtagtttc gacaccgaa gcggaaatg ttggaggaa tgggcggaa tttgtaaga gaaacagat ccagacata ttcaccggt tacaacata aataacttt gactttccg tatctgatt gaacgtgca aatcacttg aaattaaag cgtttcgat tatttggga cgtattctt gacattagg tctagcata aaagaaaca gtcacgcaa acaaaaatc ggtaaaaga acattcaag tcgattaat tttgaagga cgcgtagca tatgatatg ttaatggta attaagcgc gattttaag cttcgatca tatacgtta aacagcgtt tctcaagaa atattagga gagcagaag gaagatgta cattatagt gttattacg gatttgcaa aacggcgac gaacagaca agaagaaga cttgcagtg tactgttta aaagatgcg gatttacca gttagaata ctgagtaca gttcgaagt tttaccaat gatgtcgaa atggcaaga gtcaccggt gtacctata actagcttg ttgacaaaa ggggagcaa gttaaagtt gtggcccaa ttattgaaa catgctcga gctgctgga tatttcatg ccaacacat caaggagca ccttcttct gaacagtat gaaggagct acagttata gaaccgaga cgaggttac tacactgat ccaatagcc actcttgat ttcagttct ctatatccc agtattatg atagcacat aatttgtgt tatacgact ttgattaaa cccagtatc aaagaaaag ctctgttta agtgacgag caagttaca accactcca gcaaattgt atgtttgtg aagtcatcg gttagaccc ggtttgtta ccacacatc ttgcaacat cttcttgca gcgcgtaaa aaaaccaaa gctgagtta aaggaagca acagatcct gttgtaaga gccgttttg gacggtcgg caattggct tacaaaatt tcagctaac tcggtttat ggttttacg ggagctcaa gtcggaaaa ttgccttgt ttggaaatt tcaagtagt gtaactgct tatggtcga acaatgatt gaacagacg aaacaagaa gttgaacaa cattatcgc attgaaaat ggttacgaa tccgatgcg atggttatt tatggggac actgactcg gttatggtt aacttccga gtgaagtct ttagaaaga agtatggag ctggggagg gaagcagct gatttgata agtgccaag tttataaag cccatcaag ttagaattt gaaaaggtt tattggcca tacttattg attaataaa aagcgttat gctggattg tattttaca aaaccggat aaatacgac aaaatggat tgcaaaggt attgaaacc gtacgaagg gataattgt acattggtt gttgatgta attaattcg tgtctgcga aaacttctg attgataaa gatccagat ggtgctgtt aattacgcc aaaaaagtt atttcggat ttgctccaa aacaatgta gatatttct caattggtg attacgaaa gaacttaca aaaaatgat tacgctgcc aaacaggca catgttgaa ttatcgaaa aaaatgact aaacgagat cctggaaat gctcctaaa ttgggagat cgcatacct tatgttata attgcagga acgaagaat tccaaagct tttgaaaaa gcagaggat cctatctac gttttggag aacaacatt ccaattgat tcgcagtat tacttgcaa aatcaattg tctaaacct ttgattcgc atttttcaa ccgattttg ggagacagt gctgagtca attttgcta cggggtgaa cacacacgg actaaaaca atggtcact tctcgagtg ggtgcttta tcagcattt actaaaaag aaagagact tgtctgggg tgtcgttcg gttttacca gctggttat gaagcagaa gctgtttgt agtcactgt aaaccaaag gaggcaacg ttgtatcaa caggaattg ggacatcac agaatgtta gaaaatcgt ttttcggaa ctttttacc gagtgccag agatgccag ggatctttg catgaggag attttgtgc acaagtcgt gactgtcca attttttat atacggaag aaggttcaa atggaattg gatgcaagc aaagggaaa atttcgaga tttggtgat cctattatg gaggaatgg tga
- >TC004992 RA
ggaagaaac tgaaatatc ttgtttgga gtccgtttg tttacagca aaatggcaa caaaacgca atcttccgc ctccgaatt catcaggga agaggttca agaacgaca aaatttttc gccccccag ttggcgatt taaacccca ctgatgatg cactaattt ttcaagaat tagagttgg atcattaca tttcagccc cttatcctg gaatgccag gggctcaaa ctggtactg ttcctgtga ttcgaatgt ttggtatta caaaagtgg gcaatagtg tgtgttgtc atattcatg gtttttctc catactttt acattaatc tgccgaatg attttacac aagcggatt tgtcccgtt tcaaggaaa agttaaatg aagctgtgg taagcgata tgcgctcaa accgtgaca acataaccg aagcagttt taatggtgg aactggtta gaggaaaat ctctcgtag agtatcaag gagaggacg acaacaact ttgctaaaa taaccctag ccttaccga aatttattg ctgcatcta aacgtctcc taagcacta cagtcgtct atcaaccgc ttgcccatc acacgtttc atttctttg aagcaaatg tggatattg aaacgcgtt ttatggttg atacgaata ttttgggtt gttgctgga ttgagttgc cagcaggca aatggttca aacgaccag aaaataagt tccctgtaa cttcacgtt gccaaatag aagtggaca tctcatgga ctgacttta tttcacatt caccagaag gtgaatggt caccagtgg ctcgatttc gtattcata gtttcgata ttgaatgtg ctggaagaa aagggattt ttccggagc cgaacgttg atcccatta ttcaaattg ccaacgtga ttaagttac atggtgacg aggatgtgg ttactcgaa atattttca ctttaaaca gttgcgcac ccataggac atgctgagg ttcgtagtt tcgacaccg aagcggaaa tgttggagg aatgggcgg aatttgtaa gagaaacag atccagaca tattcaccg gttacaaca taaataact ttgactttc cgtatctga ttgaacgtg caaatcact tgaaattaa agcgtttcg attatttgg gacgtattc ttgacatta ggtctagca taaaagaaa cagtcacgc aaacaaaaa tcggtaaaa gaacattca agtcgatta attttgaag gacgcgtag catatgata tgttaatgg taattaagc gcgatttta agcttcgat catatacgt taaacagcg tttctcaag aaatattag gagagcaga aggaagatg tacattata gtgttatta cggatttgc aaaacggcg acgaacaga caagaagaa gacttgcag tgtactgtt taaaagatg cggatttac cagttagaa tactgagta cagttcgaa gttttacca atgatgtcg aaatggcaa gagtcaccg gtgtaccta taactagct tgttgacaa aaggggagc aagttaaag ttgtggccc aattattga aacatgctc gagctgctg gatatttca tgccaacac atcaaggag caccttctt ctgaacagt atgaaggag ctacagtta tagaaccga gacgaggtt actacactg atccaatag ccactcttg atttcagtt ctctatatc ccagtatta tgatagcac ataatttgt gttatacga ctttgatta aacccagta tcaaagaaa agctctgtt taagtgacg agcaagtta caaccactc cagcaaatt gtatgtttg tgaagtcat cggttagac ccggtttgt taccacaca tcttgcaac atcttcttg cagcgcgta aaaaaacca aagctgagt taaaggaag caacagatc ctgttgtaa gagccgttt tggacggtc ggcaattgg cttacaaaa tttcagcta actcggttt atggtttta cgggagctc aagtcggaa aattgcctt gtttggaaa tttcaagta gtgtaactg cttatggtc gaacaatga ttgaacaga cgaaacaag aagttgaac aacattatc gcattgaaa atggttacg aatccgatg cgatggtta tttatgggg acactgact cggttatgg ttaacttcc gagtgaagt ctttagaaa gaagtatgg agctgggga gggaagcag ctgatttga taagtgcca agtttataa agcccatca agttagaat ttgaaaagg tttattggc catacttat tgattaata aaaagcgtt atgctggat tgtatttta caaaaccgg ataaatacg acaaaatgg attgcaaag gtattgaaa ccgtacgaa gggataatt gtacattgg ttgttgatg taattaatt cgtgtctgc gaaaacttc tgattgata aagatccag atggtgctg ttaattacg ccaaaaaag ttatttcgg atttgctcc aaaacaatg tagatattt ctcaattgg tgattacga aagaactta caaaaaatg attacgctg ccaaacagg cacatgttg aattatcga aaaaaatga ctaaacgag atcctggaa atgctccta aattgggag atcgcatac cttatgtta taattgcag gaacgaaga attccaaag cttttgaaa aagcagagg atcctatct acgttttgg agaacaaca ttccaattg attcgcagt attacttgc aaaatcaat tgtctaaac ctttgattc gcatttttc aaccgattt tgggagaca gtgctgagt caattttgc tacggggtg aacacacac ggactaaaa caatggtca cttctcgag tgggtgctt tatcagcat ttactaaaa agaaagaga cttgtctgg ggtgtcgtt cggttttac cagctggtt atgaagcag aagctgttt gtagtcact gtaaaccaa aggaggcaa cgttgtatc aacaggaat tgggacatc acagaatgt tagaaaatc gtttttcgg aacttttta ccgagtgcc agagatgcc agggatctt tgcatgagg agattttgt gcacaagtc gtgactgtc caatttttt atatacgga agaaggttc aaatggaat tggatgcaa gcaaaggga aaatttcga gatttggtg atcctatta tggaggaat ggtgatgtt ttttttaca tgattttgt attgttagg ttgaacgcc ttttacttt aaatttatg cttttataa taaaaaagt ttcaattat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
