Status
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Gene information
General information
TC004983 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450958.1: 3155..14506
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004983
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00792

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004983 RA
      MVNTRRTES IGSTPDFNT QHRPGKTAA ADSDSSSVS 
      ESEDSQSED ASWESEDKT KTHSKKDPA LGPGVRELR 
      SRFVYSERK YSSSLRSDN LNTRKRIAL DARHSLTVY 
      SSRNMHNDS DMYNGNDSD DDMYSRKPR RSRHLRSHL 
      DRIERRTAL SLRPISQKC YADSMSDTE LRRSKRQRK 
      VQFENMSWL ADDQMHKMG YPNLNTPGY SEEDSRDAP 
      DVRLEEPSR STRHSNDVN SGKRREIKS PKYLYNYET 
      NDVPPRRRR SEITSRSSE RNQKELRNR RESNDKENN 
      EETNINGRS SHSRSSKRT SEAAGEDSE KKSDDTVED 
      DSNDSKIQE NGVETNQEG AKVEENKSS ESDLPKVRT 
      RPKPSNATI ILDRNFSRR PKNRKPFDS SSSESEGVK 
      KGYSLRARQ PKPPPMKHS ALHSHELPR NRKRFTRRR 
      KVSSSSSSS SSDADVRHS KTPKNQHSK SEQKMKSGA 
      GGSKIIPIG PETLDTKVR FSSIGGLDG HIQCLKEMI 
      LLPMMYPEV FRQFQIQPP RGVLFHGPP GTGKTLIAR 
      ALANECSFG CRKVSFFMR KGADLLSKW IGESEKQLR 
      LLFEQAAEL HPSIIFFDE LDGLAPVRS SRQDQVHAS 
      IVSTLLALM DGLDNRGEV IVIGATNRI DAIDPALRR 
      PGRFDRELF FPLPAKQER ESILKVHVS QWSSPPSEE 
      LLSYLAETA VGYCGSDLR ALCSEAVIQ GFRRTYPQV 
      YNADYRLML NPENVKVEK IDFLRAKSL LVPASHRVT 
      QGLGRKLLV ILEPLLSEA VKNVFKMLE QTFPHALNP 
      ALSKVKLSP NLRPAQLLL LGETPDCGQ TSHLAPAIL 
      YKMEHIHAY ILDLATLYR ETGRSAEET CIQVFNEAR 
      RNVPSVIYI PNIDNWWAL VSETVKAIL DAQLSQMDP 
      NIPILLLAT AETTYDNLP DEIQNIFSR YRKEVYQLD 
      LPNDASREA FFKPLLIDS CLKPHRPPR KRPKTPPPL 
      PRARTPPPT PLTEEQSKK LYEQEEHTL RELRIFLRD 
      ICKKLANNK LFYMFTKPV DTEEVPDYP TIIKQPMDL 
      ETMMTKVDF HRYECAKDF LKDIELIVH NALEYNPAK
    CDS sequence
    • >TC004983 RA
      atggtgaat actcgtcgt acggagtca attggctcg 
      acgccagac ttcaacaca cagcatcgt ccagggaaa 
      acagcagct gcagactca gactcatcc agtgttagc 
      gaaagtgaa gattctcaa agtgaggac gcttcatgg 
      gagtccgaa gacaagacc aagacccac tccaagaag 
      gatccggcc ttaggaccc ggagtcaga gaactgcga 
      agcagattt gtttatagc gaaagaaaa tattcttcc 
      agcttacgc agtgataat ttaaataca cggaaacga 
      atagcactt gatgcgaga cattctctt acagtctat 
      tcatcaagg aatatgcac aatgattca gacatgtac 
      aatggaaat gattcggat gacgacatg tattcccga 
      aaaccaagg cgatcacgt cacttaagg tcgcattta 
      gataggatt gaacgtagg actgcgctg tcgctgcgc 
      cccatcagc caaaaatgc tacgccgac tccatgtcc 
      gacaccgaa ctgaggcgc agcaagcgg cagcgcaag 
      gtccaattc gaaaacatg tcgtggctt gccgacgat 
      cagatgcac aaaatgggt tatcccaac ttaaataca 
      cctggatat tcggaggaa gacagtcgt gatgcccca 
      gatgttcgc ttagaagaa cccagcaga agcactcgt 
      cattcaaac gatgtaaat agcggcaaa cgtagagaa 
      ataaaaagc cccaagtat ttatacaac tacgaaacg 
      aatgatgtc cctccacga agacgtcga agcgagata 
      acgtctcgt tcgagcgaa cgcaatcaa aaagaacta 
      cgaaatagg cgcgaatcg aacgataaa gaaaacaac 
      gaagaaact aacattaat ggacgatca tcacatagt 
      cggtcttcg aagaggaca agtgaggcc gcaggggag 
      gatagtgag aagaaaagc gatgatact gtggaagat 
      gattccaat gacagcaag atacaagaa aatggggtt 
      gaaacgaat caagaaggg gccaaagtt gaagaaaat 
      aaatcatca gagagtgat ctcccgaag gtgcggaca 
      agaccaaaa ccgtcgaat gccaccatc atactggat 
      cggaatttt tcgcggagg cctaagaat cgcaagccc 
      tttgatagt tcaagttcc gaatctgag ggcgttaag 
      aagggctac tcgctacga gctcggcaa ccgaaaccg 
      cccccaatg aaacattcg gcgttacat tcacacgaa 
      cttcccaga aaccggaag cggttcact cgacgaaga 
      aaagtttca tcatcgagt tcgagttct tcatcagat 
      gcggacgta cggcatagt aaaacaccc aaaaatcag 
      cattccaag agtgagcaa aaaatgaaa tctggcgcc 
      ggtggtagt aaaattata ccaatcggg cctgaaacc 
      ttagacact aaagttagg tttagtagt attggaggt 
      ctagatggc cacatccag tgtctcaaa gaaatgatt 
      ctactgcct atgatgtat cccgaagtg ttcagacaa 
      ttccaaata caaccaccc agaggtgtt ctctttcat 
      gggcctcca ggtactggc aaaactcta atcgcgcgt 
      gctctggct aatgaatgc agttttggt tgccgcaag 
      gtgtcgttt tttatgcga aaaggagca gatttgctc 
      agtaaatgg attggtgaa tcggaaaaa caactacga 
      ttgcttttc gaacaagcc gctgagtta catccttcg 
      attattttt ttcgacgag ttggacggt ttagctcct 
      gtgaggtct tccagacag gatcaggtt catgctagt 
      attgtttca acgttgctt gctttgatg gacggtttg 
      gataatcgt ggtgaagta atagtaata ggagcgacg 
      aatcggata gatgccatt gatcctgcg ctcagaaga 
      ccagggagg ttcgatcgg gagctgttt tttcctctt 
      cctgcgaaa caagaacgt gagtccatt ttgaaggtg 
      cacgtctcg cagtggtcc tctccgcca agtgaggag 
      cttttatct tacttggca gaaacggcg gtcggctat 
      tgcggctcc gacttgcgt gctttgtgt tcggaagcc 
      gttatacaa gggttcaga cgaacatat cctcaagtt 
      tacaatgca gactatcga ctgatgtta aatccggag 
      aacgttaaa gtcgaaaag atcgatttt ttaagagcc 
      aaatctctc ctagtacct gcctcgcat cgggtcact 
      caaggccta ggacgcaaa ctgttggta atcctagaa 
      ccattgttg agtgaagcc gttaaaaac gttttcaaa 
      atgcttgaa caaacgttt ccgcatgct ttaaaccct 
      gctctctcc aaagttaaa ttatctccg aatttacga 
      ccagctcag cttctcctc ttgggagaa acgcccgac 
      tgtggtcaa acttcacat ctggcgcct gcgatattg 
      tacaaaatg gaacatatc catgcctac attctcgat 
      ctagcgact ttgtataga gaaacgggt cgttcagct 
      gaagaaacg tgcattcaa gtgtttaat gaggcgcga 
      cgaaacgtt ccaagtgtg atctacatt ccaaacatt 
      gataattgg tgggctttg gttagcgaa acagtgaag 
      gcgattttg gatgcgcaa ttgtcgcaa atggatccg 
      aatattccc attttgtta ctagcaacg gccgagaca 
      acctacgat aatctacca gacgaaatt caaaatatt 
      ttctcccgc taccggaag gaagtttac caactggat 
      ttgccaaac gatgcaagt cgtgaagca tttttcaaa 
      ccattgttg atcgattcg tgtttgaag ccacacaga 
      ccgccgagg aaaaggccc aagacaccg ccacctctt 
      ccgagagct cgcacccct ccacctacg cctctaacc 
      gaagaacag tcgaaaaaa ttatacgaa caagaggaa 
      cacactctc agggagctt aggatattt ttaagagat 
      atttgtaaa aagttagcc aataacaaa ttgttctat 
      atgttcacc aaaccagtc gacactgaa gaagtgcct 
      gattatccc accattatc aaacagccc atggatctg 
      gagacgatg atgaccaaa gtcgatttt caccgatac 
      gaatgtgcc aaagatttt ctaaaggat atcgagctt 
      atcgttcac aacgccctc gagtataat ccagccaag 
      taa
    mRNA sequence
    • >TC004983 RA
      gcgccgttt ggtgaaaga cgtgaatgg ctgtgtgtt 
      ttgttcatt gtcctgatt tttagaaga aaaacacct 
      cagaattta gttttttaa cagaaattc tgacggtgg 
      tgtgatggt gaatactcg tcgtacgga gtcaattgg 
      ctcgacgcc agacttcaa cacacagca tcgtccagg 
      gaaaacagc agctgcaga ctcagactc atccagtgt 
      tagcgaaag tgaagattc tcaaagtga ggacgcttc 
      atgggagtc cgaagacaa gaccaagac ccactccaa 
      gaaggatcc ggccttagg acccggagt cagagaact 
      gcgaagcag atttgttta tagcgaaag aaaatattc 
      ttccagctt acgcagtga taatttaaa tacacggaa 
      acgaatagc acttgatgc gagacattc tcttacagt 
      ctattcatc aaggaatat gcacaatga ttcagacat 
      gtacaatgg aaatgattc ggatgacga catgtattc 
      ccgaaaacc aaggcgatc acgtcactt aaggtcgca 
      tttagatag gattgaacg taggactgc gctgtcgct 
      gcgccccat cagccaaaa atgctacgc cgactccat 
      gtccgacac cgaactgag gcgcagcaa gcggcagcg 
      caaggtcca attcgaaaa catgtcgtg gcttgccga 
      cgatcagat gcacaaaat gggttatcc caacttaaa 
      tacacctgg atattcgga ggaagacag tcgtgatgc 
      cccagatgt tcgcttaga agaacccag cagaagcac 
      tcgtcattc aaacgatgt aaatagcgg caaacgtag 
      agaaataaa aagccccaa gtatttata caactacga 
      aacgaatga tgtccctcc acgaagacg tcgaagcga 
      gataacgtc tcgttcgag cgaacgcaa tcaaaaaga 
      actacgaaa taggcgcga atcgaacga taaagaaaa 
      caacgaaga aactaacat taatggacg atcatcaca 
      tagtcggtc ttcgaagag gacaagtga ggccgcagg 
      ggaggatag tgagaagaa aagcgatga tactgtgga 
      agatgattc caatgacag caagataca agaaaatgg 
      ggttgaaac gaatcaaga aggggccaa agttgaaga 
      aaataaatc atcagagag tgatctccc gaaggtgcg 
      gacaagacc aaaaccgtc gaatgccac catcatact 
      ggatcggaa tttttcgcg gaggcctaa gaatcgcaa 
      gccctttga tagttcaag ttccgaatc tgagggcgt 
      taagaaggg ctactcgct acgagctcg gcaaccgaa 
      accgccccc aatgaaaca ttcggcgtt acattcaca 
      cgaacttcc cagaaaccg gaagcggtt cactcgacg 
      aagaaaagt ttcatcatc gagttcgag ttcttcatc 
      agatgcgga cgtacggca tagtaaaac acccaaaaa 
      tcagcattc caagagtga gcaaaaaat gaaatctgg 
      cgccggtgg tagtaaaat tataccaat cgggcctga 
      aaccttaga cactaaagt taggtttag tagtattgg 
      aggtctaga tggccacat ccagtgtct caaagaaat 
      gattctact gcctatgat gtatcccga agtgttcag 
      acaattcca aatacaacc acccagagg tgttctctt 
      tcatgggcc tccaggtac tggcaaaac tctaatcgc 
      gcgtgctct ggctaatga atgcagttt tggttgccg 
      caaggtgtc gttttttat gcgaaaagg agcagattt 
      gctcagtaa atggattgg tgaatcgga aaaacaact 
      acgattgct tttcgaaca agccgctga gttacatcc 
      ttcgattat ttttttcga cgagttgga cggtttagc 
      tcctgtgag gtcttccag acaggatca ggttcatgc 
      tagtattgt ttcaacgtt gcttgcttt gatggacgg 
      tttggataa tcgtggtga agtaatagt aataggagc 
      gacgaatcg gatagatgc cattgatcc tgcgctcag 
      aagaccagg gaggttcga tcgggagct gttttttcc 
      tcttcctgc gaaacaaga acgtgagtc cattttgaa 
      ggtgcacgt ctcgcagtg gtcctctcc gccaagtga 
      ggagctttt atcttactt ggcagaaac ggcggtcgg 
      ctattgcgg ctccgactt gcgtgcttt gtgttcgga 
      agccgttat acaagggtt cagacgaac atatcctca 
      agtttacaa tgcagacta tcgactgat gttaaatcc 
      ggagaacgt taaagtcga aaagatcga ttttttaag 
      agccaaatc tctcctagt acctgcctc gcatcgggt 
      cactcaagg cctaggacg caaactgtt ggtaatcct 
      agaaccatt gttgagtga agccgttaa aaacgtttt 
      caaaatgct tgaacaaac gtttccgca tgctttaaa 
      ccctgctct ctccaaagt taaattatc tccgaattt 
      acgaccagc tcagcttct cctcttggg agaaacgcc 
      cgactgtgg tcaaacttc acatctggc gcctgcgat 
      attgtacaa aatggaaca tatccatgc ctacattct 
      cgatctagc gactttgta tagagaaac gggtcgttc 
      agctgaaga aacgtgcat tcaagtgtt taatgaggc 
      gcgacgaaa cgttccaag tgtgatcta cattccaaa 
      cattgataa ttggtgggc tttggttag cgaaacagt 
      gaaggcgat tttggatgc gcaattgtc gcaaatgga 
      tccgaatat tcccatttt gttactagc aacggccga 
      gacaaccta cgataatct accagacga aattcaaaa 
      tattttctc ccgctaccg gaaggaagt ttaccaact 
      ggatttgcc aaacgatgc aagtcgtga agcattttt 
      caaaccatt gttgatcga ttcgtgttt gaagccaca 
      cagaccgcc gaggaaaag gcccaagac accgccacc 
      tcttccgag agctcgcac ccctccacc tacgcctct 
      aaccgaaga acagtcgaa aaaattata cgaacaaga 
      ggaacacac tctcaggga gcttaggat atttttaag 
      agatatttg taaaaagtt agccaataa caaattgtt 
      ctatatgtt caccaaacc agtcgacac tgaagaagt 
      gcctgatta tcccaccat tatcaaaca gcccatgga 
      tctggagac gatgatgac caaagtcga ttttcaccg 
      atacgaatg tgccaaaga ttttctaaa ggatatcga 
      gcttatcgt tcacaacgc cctcgagta taatccagc 
      caagtaagt ttttctttt aagacccgt gaattttgt 
      caataaaaa aaatgaaaa aaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY