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Gene information
General information
TC004978 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5449577..5451031
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004978
    iBeetle screen iB_00791

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004978 RA
      MGDSKKTAP CVYSRDNDK CQDEKLLQP FTYIMQTPG 
      KNIRSKLAR AFNYWLNIS EEKLKAIEE IVEMMHNAS 
      LLLDDIQDN SILRRGVPV AHSIYGVPS TINAANYVI 
      FIALQRVLE LNHPEAVSV YTQQVLELH RGQGMEIYW 
      RDSYTCPTE DEYKQMTIR KTGGLFMLA IRLMQLFSE 
      NKADYTKLT GILGLFFQI RDDFLNLYS KDYMLHKSY 
      CEDLTEGKF SFPIIHAIH SQPNDNQVI HILRQRTKD 
      NEVKKYCVS LLEKYGSFD YTKKVLAEL KQEAQDEVD 
      KLGENPYMA EVMEYIFKL E
    CDS sequence
    • >TC004978 RA
      atgggtgat tcgaaaaaa acagcccct tgtgtgtac 
      agccgagac aacgacaag tgccaagat gagaaattg 
      ctacaaccg ttcacttac atcatgcaa actccgggg 
      aagaacatc cggtcgaaa ttagctcga gctttcaac 
      tattggttg aatatcagt gaagagaag ctgaaggcg 
      attgaggag atagttgaa atgatgcat aacgctagt 
      ctccttttg gatgatatc caggataat tcaatactg 
      agaagagga gtacctgta gctcactca atttatggg 
      gttcctagc accataaat gccgccaat tatgtcatt 
      tttattgca ttgcaaaga gttttagaa ctgaatcac 
      cctgaagcg gtaagtgtc tacacacaa caagttttg 
      gagctccac agaggtcaa ggcatggaa atttattgg 
      cgagacagc tacacctgt cccacggaa gatgaatac 
      aaacagatg acgattaga aaaactggg ggccttttt 
      atgttagca attagatta atgcaacta ttcagtgaa 
      aataaggct gattatacc aaactaacg gggattttg 
      ggactgttt ttccaaatt cgggacgat tttctaaac 
      ctatattct aaagattat atgttacac aagagctat 
      tgcgaggac ctaactgag ggcaaattt agttttccg 
      ataattcac gcaattcac agccaacca aatgataat 
      caagtaatt catatttta aggcaaaga actaaagac 
      aatgaagta aagaaatac tgcgtttcg ttgcttgag 
      aaatacgga agttttgat tacacgaag aaagtgttg 
      gcagaactg aagcaagag gcccaagat gaagttgat 
      aagttgggg gaaaacccg tacatggcc gaagtaatg 
      gagtatatt tttaagttg gaataa
    mRNA sequence
    • >TC004978 RA
      gtacttcca aattttcta accgcaaaa aacacccat 
      taatttttt agtttttcc aaaaaaaac acgtatcgt 
      ttgcataaa cgcctcaaa atgggtgat tcgaaaaaa 
      acagcccct tgtgtgtac agccgagac aacgacaag 
      tgccaagat gagaaattg ctacaaccg ttcacttac 
      atcatgcaa actccgggg aagaacatc cggtcgaaa 
      ttagctcga gctttcaac tattggttg aatatcagt 
      gaagagaag ctgaaggcg attgaggag atagttgaa 
      atgatgcat aacgctagt ctccttttg gatgatatc 
      caggataat tcaatactg agaagagga gtacctgta 
      gctcactca atttatggg gttcctagc accataaat 
      gccgccaat tatgtcatt tttattgca ttgcaaaga 
      gttttagaa ctgaatcac cctgaagcg gtaagtgtc 
      tacacacaa caagttttg gagctccac agaggtcaa 
      ggcatggaa atttattgg cgagacagc tacacctgt 
      cccacggaa gatgaatac aaacagatg acgattaga 
      aaaactggg ggccttttt atgttagca attagatta 
      atgcaacta ttcagtgaa aataaggct gattatacc 
      aaactaacg gggattttg ggactgttt ttccaaatt 
      cgggacgat tttctaaac ctatattct aaagattat 
      atgttacac aagagctat tgcgaggac ctaactgag 
      ggcaaattt agttttccg ataattcac gcaattcac 
      agccaacca aatgataat caagtaatt catatttta 
      aggcaaaga actaaagac aatgaagta aagaaatac 
      tgcgtttcg ttgcttgag aaatacgga agttttgat 
      tacacgaag aaagtgttg gcagaactg aagcaagag 
      gcccaagat gaagttgat aagttgggg gaaaacccg 
      tacatggcc gaagtaatg gagtatatt tttaagttg 
      gaataattt tttcatgat caaattaaa ttattgtga 
      tatctatat taagttgct acaataaac actatttca 
      tcga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY