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Gene information
General information
TC004950 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4981528..4982826
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004950
    iBeetle screen iB_00789

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004950 RA
      MSKTLKLNN GLEIPVVGL GTYKSGKGE VKRAVSDAI 
      DAGYRHFDC AWFYGNEDE VGVALNEKI KQGVVKRED 
      LFVTSKLWN NFHARDKVV PMCKETLSS LKLSYIDLY 
      LVHWPFGFK ESASLWPIN EGEAAFSDI DYLETWQGM 
      EECVRLGLT KSIGVSNFN EEQIERLIK NCKIKPVVN 
      QVEVNPNLN QKKLIEFCK KHDIVVTGY CPLGRSEYA 
      GTPGFPEPT IFDPKVIEM GKKYKKTPA QIVLNYLVS 
      LGISVVPKS VTKSRIIEN IDIFDFKLD AGDVAYLDS 
      CNKNQRVCP LSEFSAHKY YSF
    CDS sequence
    • >TC004950 RA
      atgagtaaa accttaaag ctcaacaac ggccttgag 
      attccggtt gtgggtctg gggacatac aagtcggga 
      aaaggtgaa gtaaaaagg gcggtctca gacgctata 
      gacgccggt tatcgtcac tttgactgc gcttggttt 
      tacggcaat gaggacgaa gttggggtt gcgctcaat 
      gaaaaaatt aaacaaggg gttgttaaa cgggaagat 
      ttatttgta acgagcaaa ctatggaac aatttccat 
      gcgagggac aaggtggtt cccatgtgc aaggaaact 
      ttgagcagt ttgaaactg tcctacatc gatttatat 
      ctcgtgcat tggccattt ggattcaag gaaagtgcg 
      tctttgtgg ccaataaac gaaggcgaa gccgctttc 
      agcgacatt gactatctc gaaacctgg caagggatg 
      gaagaatgc gtccgtctg ggtctcaca aaaagcatc 
      ggagtgtcc aacttcaac gaggaacaa atcgagcgt 
      ctcatcaaa aattgtaaa atcaagccc gttgtcaac 
      caagtcgaa gtcaacccg aatttaaac cagaaaaaa 
      ttgatcgaa ttttgcaaa aaacacgac attgtcgtt 
      accggttac tgtcctttg gggcgtagc gaatatgct 
      ggaaccccg ggattcccc gaacctaca atcttcgac 
      ccgaaagtc atcgaaatg ggcaagaaa tacaagaaa 
      acgccggca caaatcgtc ttaaattac ttggtttct 
      ttgggaatt agtgtggtc ccgaaatcc gtcacgaaa 
      tcccggatt atcgaaaat atcgatatt ttcgatttc 
      aagttggat gcgggtgac gtcgcctac ttggattcg 
      tgcaataag aatcaaaga gtgtgtcct ttgagcgag 
      ttttcggca cacaaatat tatagtttt tga
    mRNA sequence
    • >TC004950 RA
      gtgatatta taaacacag aaaactcca gcacaagtg 
      ataagctct gctgaacgt tgaaatcga tcttccttc 
      gttttgcca ggaacagta cacaggcat aatgagtaa 
      aaccttaaa gctcaacaa cggccttga gattccggt 
      tgtgggtct ggggacata caagtcggg aaaaggtga 
      agtaaaaag ggcggtctc agacgctat agacgccgg 
      ttatcgtca ctttgactg cgcttggtt ttacggcaa 
      tgaggacga agttggggt tgcgctcaa tgaaaaaat 
      taaacaagg ggttgttaa acgggaaga tttatttgt 
      aacgagcaa actatggaa caatttcca tgcgaggga 
      caaggtggt tcccatgtg caaggaaac tttgagcag 
      tttgaaact gtcctacat cgatttata tctcgtgca 
      ttggccatt tggattcaa ggaaagtgc gtctttgtg 
      gccaataaa cgaaggcga agccgcttt cagcgacat 
      tgactatct cgaaacctg gcaagggat ggaagaatg 
      cgtccgtct gggtctcac aaaaagcat cggagtgtc 
      caacttcaa cgaggaaca aatcgagcg tctcatcaa 
      aaattgtaa aatcaagcc cgttgtcaa ccaagtcga 
      agtcaaccc gaatttaaa ccagaaaaa attgatcga 
      attttgcaa aaaacacga cattgtcgt taccggtta 
      ctgtccttt ggggcgtag cgaatatgc tggaacccc 
      gggattccc cgaacctac aatcttcga cccgaaagt 
      catcgaaat gggcaagaa atacaagaa aacgccggc 
      acaaatcgt cttaaatta cttggtttc tttgggaat 
      tagtgtggt cccgaaatc cgtcacgaa atcccggat 
      tatcgaaaa tatcgatat tttcgattt caagttgga 
      tgcgggtga cgtcgccta cttggattc gtgcaataa 
      gaatcaaag agtgtgtcc tttgagcga gttttcggc 
      acacaaata ttatagttt ttgaaataa agagttgtt 
      ttgtgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY