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Gene information
General information
TC004949 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4969373..4979486
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004949
    iBeetle screen iB_00788

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004949 RA
      MGDMFRSEQ MVLAQLFIQ PEAAYFAIS ELGESGIVQ 
FRDLNENVN VFQRKFVNE VRRCDEMER KLRYIEAEV 
KKDNVAIPD QSELPKAPN PREIIDLEA HLEKTEGDI 
KELSESAVN LKSNYLELI ELKQVLEKT QAFFNEQDE 
ANGLDSAHK ALINDESHN VSIRGRLGF VAGVINRER 
VPGFERMLW RISRGNVFL RQVEIEKPL EDPATGNQL 
YKTVFVAFF QGEQLKTRI KKVCAGYHA SLYACPSSL 
QERNEMLKG VCTRLEDLN LVLNQTQDH RQRVLVSVA 
KELQNWSVM VSKMKAIYH TLNFFNMDV TKKCLIGEC 
WVSSKDIPI VQKALSDGS SACGSSIPS FLNVINTNE 
DPPTFNRTN KFTRGFQNL IDSYGVASY REANPALYT 
IITFPFLFA VMFGDVGHA MIMALFGGY LVISEKKIM 
AKRSNNEIF NIFFAGRYI ILLMGLFSM YTGFVYNDI 
FSKSMNLFG SSWFVQFNK EQALELDER DLDPRYDYT 
GTPYFIGMD PAWQLAKNK IIFLNSYKM KLSIIFGVV 
HMIFGVCVS VVNFVHFRK YSSIFLEFL PQILLLCFL 
FLWMVVMMF MKWIIYSAD LGKPVELGT SCAPNVLIY 
FINMMLFKA TESPEGCKD YMFEGQQTV QQILVFLSL 
ACIPVLLLG KPLYIKCTR KSKPHVRSN GDVNQGMEL 
GEYPEAQQN AAATSHEDE EEEEPMSEI FIHQAIHTI 
EYVLSTISH TASYLRLWA LSLAHAQLS EVLWSMLFR 
MGLTNSSYV GAITAFVFF AAWAAFTIA ILVTMEGLS 
AFLHTLRLH WVEFMSKFY AGLGYPFQP FSFKAILEE 
ENAPEE
    CDS sequence
    • >TC004949 RA
      atgggggac atgttccgc agtgagcaa atggtactt 
gctcagctc ttcatccaa cctgaggct gcatatttc 
gccatatcg gagctcgga gaatccgga atcgttcaa 
ttccgagac ttgaatgaa aacgtgaac gtgttccaa 
cgaaaattc gtaaacgag gtccgaaga tgcgacgaa 
atggagcgc aagctgcgc tacatcgag gccgaggtg 
aagaaagac aatgtggcg attcccgac cagtctgag 
ctccccaaa gctccaaat cccagggag attattgat 
ctggaagcc caccttgag aaaaccgaa ggcgatatc 
aaggagttg tccgaaagc gcggttaac ctgaagtcc 
aattatctg gagctgatc gagttgaag caagtgctt 
gagaaaact caagctttc tttaacgag caagacgaa 
gccaacggt ttagattcg gcccacaag gctttgatc 
aatgacgaa agtcacaat gtgtccatt aggggccgt 
ttggggttc gttgcgggg gttatcaac cgggagcga 
gtgccaggt ttcgagaga atgctctgg aggatatcc 
agagggaac gtttttttg agacaggtc gagattgaa 
aaaccgctc gaagatccg gccacgggg aatcaactg 
tacaaaacc gttttcgtt gcgtttttc caaggcgag 
cagttgaaa acaaggatt aagaaagtt tgtgctgga 
tatcacgcc tcgctttat gcgtgtcca agctcgctt 
caagaacgc aacgagatg cttaaaggt gtctgcacg 
aggttggaa gatttgaat ttggttcta aaccaaacg 
caagaccac cggcaaaga gtgctagtg agtgtggcc 
aaggaattg caaaattgg agcgtgatg gttagcaaa 
atgaaggcg atttatcac actttgaat ttctttaat 
atggacgtg acgaaaaaa tgcttgatt ggggagtgt 
tgggtcagc agtaaagac attccaatc gtacagaag 
gccctgtcg gatggttcc agtgcttgc ggcagctcc 
attccgtca tttttgaac gtgattaac accaatgag 
gatcctccg acgttcaat agaacgaat aaattcacc 
agagggttc cagaatctg attgattcg tacggagtt 
gcatcgtat cgtgaagct aaccctgcc ctttacacc 
atcatcact tttccgttt ctgttcgct gtgatgttc 
ggtgacgtt ggacacgca atgatcatg gccctattt 
ggcggttat ttggtaatt tccgagaag aaaatcatg 
gccaaacgg agtaataac gaaattttc aacattttc 
ttcgccggg agatacatc attctcctc atgggtctc 
ttctcaatg tacaccggt tttgtctac aacgacatt 
ttctcaaag tccatgaac ttgttcggg tccagttgg 
tttgtccaa ttcaacaaa gaacaagcc ttggagttg 
gatgaacgc gatctggac ccccgatat gactacact 
gggaccccc tatttcatc ggcatggac cccgcgtgg 
caattggcc aaaaacaag atcattttc ctaaattcg 
tacaaaatg aaactgtct attattttc ggggtcgtt 
catatgatt ttcggcgtg tgcgtcagc gtggtcaat 
ttcgtccat ttcaggaaa tactcaagc atcttcctg 
gaattcctc ccacagatc ctcctgctg tgtttcctc 
ttcttgtgg atggtggtg atgatgttc atgaaatgg 
attatctat tcggctgat ttgggcaaa cctgttgag 
ttgggtact tcgtgcgca cccaatgtc ctcatctac 
ttcatcaac atgatgctg ttcaaagcg acagagtcg 
ccggaaggt tgcaaggat tacatgttt gagggccaa 
cagaccgtg caacaaatc ttagttttc ttgtcattg 
gcgtgtatc ccggtgctg ttgctcgga aagccgctt 
tatatcaaa tgcacgagg aaaagtaag cctcatgtg 
cgaagtaac ggcgatgtt aaccaagga atggagttg 
ggggaatac cccgaagcg cagcaaaac gcagctgcg 
acgtcgcac gaagacgag gaggaggag gaaccgatg 
agtgagatt ttcatccat caggcaatt cacacaatt 
gagtacgtt ttgagtact atttcgcac actgcgtcc 
tatttgcgg ttgtgggcc ttgtcgtta gctcacgcg 
caactttct gaagtattg tggagcatg ttgttcagg 
atgggcttg acaaatagc tcgtatgtt ggggcgatt 
acggccttt gtgtttttc gcagcgtgg gcggctttt 
acgatagcc attttagtc acaatggaa gggttatca 
gcgtttttg cacactttg cgtttgcat tgggtcgaa 
tttatgagt aagttttat gccgggttg ggatatccg 
ttccaacca ttcagtttt aaagcaatc ctcgaggaa 
gaaaatgcg ccagaggaa taa
    mRNA sequence
    • >TC004949 RA
      attgtgttg aacctgaag aaaacgtgc gtctggttg 
tagtgtgaa cgtacagtt tgtggtgaa atgactgta 
actttaaaa tcggcgctg agtgtcacg tgattgtta 
ttttgtttt tgttttatt tacgcagaa agtgaaacc 
gcctcaaaa tgggggaca tgttccgca gtgagcaaa 
tggtacttg ctcagctct tcatccaac ctgaggctg 
catatttcg ccatatcgg agctcggag aatccggaa 
tcgttcaat tccgagact tgaatgaaa acgtgaacg 
tgttccaac gaaaattcg taaacgagg tccgaagat 
gcgacgaaa tggagcgca agctgcgct acatcgagg 
ccgaggtga agaaagaca atgtggcga ttcccgacc 
agtctgagc tccccaaag ctccaaatc ccagggaga 
ttattgatc tggaagccc accttgaga aaaccgaag 
gcgatatca aggagttgt ccgaaagcg cggttaacc 
tgaagtcca attatctgg agctgatcg agttgaagc 
aagtgcttg agaaaactc aagctttct ttaacgagc 
aagacgaag ccaacggtt tagattcgg cccacaagg 
ctttgatca atgacgaaa gtcacaatg tgtccatta 
ggggccgtt tggggttcg ttgcggggg ttatcaacc 
gggagcgag tgccaggtt tcgagagaa tgctctgga 
ggatatcca gagggaacg tttttttga gacaggtcg 
agattgaaa aaccgctcg aagatccgg ccacgggga 
atcaactgt acaaaaccg ttttcgttg cgtttttcc 
aaggcgagc agttgaaaa caaggatta agaaagttt 
gtgctggat atcacgcct cgctttatg cgtgtccaa 
gctcgcttc aagaacgca acgagatgc ttaaaggtg 
tctgcacga ggttggaag atttgaatt tggttctaa 
accaaacgc aagaccacc ggcaaagag tgctagtga 
gtgtggcca aggaattgc aaaattgga gcgtgatgg 
ttagcaaaa tgaaggcga tttatcaca ctttgaatt 
tctttaata tggacgtga cgaaaaaat gcttgattg 
gggagtgtt gggtcagca gtaaagaca ttccaatcg 
tacagaagg ccctgtcgg atggttcca gtgcttgcg 
gcagctcca ttccgtcat ttttgaacg tgattaaca 
ccaatgagg atcctccga cgttcaata gaacgaata 
aattcacca gagggttcc agaatctga ttgattcgt 
acggagttg catcgtatc gtgaagcta accctgccc 
tttacacca tcatcactt ttccgtttc tgttcgctg 
tgatgttcg gtgacgttg gacacgcaa tgatcatgg 
ccctatttg gcggttatt tggtaattt ccgagaaga 
aaatcatgg ccaaacgga gtaataacg aaattttca 
acattttct tcgccggga gatacatca ttctcctca 
tgggtctct tctcaatgt acaccggtt ttgtctaca 
acgacattt tctcaaagt ccatgaact tgttcgggt 
ccagttggt ttgtccaat tcaacaaag aacaagcct 
tggagttgg atgaacgcg atctggacc cccgatatg 
actacactg ggaccccct atttcatcg gcatggacc 
ccgcgtggc aattggcca aaaacaaga tcattttcc 
taaattcgt acaaaatga aactgtcta ttattttcg 
gggtcgttc atatgattt tcggcgtgt gcgtcagcg 
tggtcaatt tcgtccatt tcaggaaat actcaagca 
tcttcctgg aattcctcc cacagatcc tcctgctgt 
gtttcctct tcttgtgga tggtggtga tgatgttca 
tgaaatgga ttatctatt cggctgatt tgggcaaac 
ctgttgagt tgggtactt cgtgcgcac ccaatgtcc 
tcatctact tcatcaaca tgatgctgt tcaaagcga 
cagagtcgc cggaaggtt gcaaggatt acatgtttg 
agggccaac agaccgtgc aacaaatct tagttttct 
tgtcattgg cgtgtatcc cggtgctgt tgctcggaa 
agccgcttt atatcaaat gcacgagga aaagtaagc 
ctcatgtgc gaagtaacg gcgatgtta accaaggaa 
tggagttgg gggaatacc ccgaagcgc agcaaaacg 
cagctgcga cgtcgcacg aagacgagg aggaggagg 
aaccgatga gtgagattt tcatccatc aggcaattc 
acacaattg agtacgttt tgagtacta tttcgcaca 
ctgcgtcct atttgcggt tgtgggcct tgtcgttag 
ctcacgcgc aactttctg aagtattgt ggagcatgt 
tgttcagga tgggcttga caaatagct cgtatgttg 
gggcgatta cggcctttg tgtttttcg cagcgtggg 
cggctttta cgatagcca ttttagtca caatggaag 
ggttatcag cgtttttgc acactttgc gtttgcatt 
gggtcgaat ttatgagta agttttatg ccgggttgg 
gatatccgt tccaaccat tcagtttta aagcaatcc 
tcgaggaag aaaatgcgc cagaggaat aacactttt 
atcagcttt ttagatagt gcacaaata gaatagcag 
caaataaaa atacttgtt aaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY