| General information | |||
| TC004949 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 4969373..4979486 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC004949 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00788 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC004949 RA
MGDMFRSEQ MVLAQLFIQ PEAAYFAIS ELGESGIVQ FRDLNENVN VFQRKFVNE VRRCDEMER KLRYIEAEV KKDNVAIPD QSELPKAPN PREIIDLEA HLEKTEGDI KELSESAVN LKSNYLELI ELKQVLEKT QAFFNEQDE ANGLDSAHK ALINDESHN VSIRGRLGF VAGVINRER VPGFERMLW RISRGNVFL RQVEIEKPL EDPATGNQL YKTVFVAFF QGEQLKTRI KKVCAGYHA SLYACPSSL QERNEMLKG VCTRLEDLN LVLNQTQDH RQRVLVSVA KELQNWSVM VSKMKAIYH TLNFFNMDV TKKCLIGEC WVSSKDIPI VQKALSDGS SACGSSIPS FLNVINTNE DPPTFNRTN KFTRGFQNL IDSYGVASY REANPALYT IITFPFLFA VMFGDVGHA MIMALFGGY LVISEKKIM AKRSNNEIF NIFFAGRYI ILLMGLFSM YTGFVYNDI FSKSMNLFG SSWFVQFNK EQALELDER DLDPRYDYT GTPYFIGMD PAWQLAKNK IIFLNSYKM KLSIIFGVV HMIFGVCVS VVNFVHFRK YSSIFLEFL PQILLLCFL FLWMVVMMF MKWIIYSAD LGKPVELGT SCAPNVLIY FINMMLFKA TESPEGCKD YMFEGQQTV QQILVFLSL ACIPVLLLG KPLYIKCTR KSKPHVRSN GDVNQGMEL GEYPEAQQN AAATSHEDE EEEEPMSEI FIHQAIHTI EYVLSTISH TASYLRLWA LSLAHAQLS EVLWSMLFR MGLTNSSYV GAITAFVFF AAWAAFTIA ILVTMEGLS AFLHTLRLH WVEFMSKFY AGLGYPFQP FSFKAILEE ENAPEE
- >TC004949 RA
atgggggac atgttccgc agtgagcaa atggtactt gctcagctc ttcatccaa cctgaggct gcatatttc gccatatcg gagctcgga gaatccgga atcgttcaa ttccgagac ttgaatgaa aacgtgaac gtgttccaa cgaaaattc gtaaacgag gtccgaaga tgcgacgaa atggagcgc aagctgcgc tacatcgag gccgaggtg aagaaagac aatgtggcg attcccgac cagtctgag ctccccaaa gctccaaat cccagggag attattgat ctggaagcc caccttgag aaaaccgaa ggcgatatc aaggagttg tccgaaagc gcggttaac ctgaagtcc aattatctg gagctgatc gagttgaag caagtgctt gagaaaact caagctttc tttaacgag caagacgaa gccaacggt ttagattcg gcccacaag gctttgatc aatgacgaa agtcacaat gtgtccatt aggggccgt ttggggttc gttgcgggg gttatcaac cgggagcga gtgccaggt ttcgagaga atgctctgg aggatatcc agagggaac gtttttttg agacaggtc gagattgaa aaaccgctc gaagatccg gccacgggg aatcaactg tacaaaacc gttttcgtt gcgtttttc caaggcgag cagttgaaa acaaggatt aagaaagtt tgtgctgga tatcacgcc tcgctttat gcgtgtcca agctcgctt caagaacgc aacgagatg cttaaaggt gtctgcacg aggttggaa gatttgaat ttggttcta aaccaaacg caagaccac cggcaaaga gtgctagtg agtgtggcc aaggaattg caaaattgg agcgtgatg gttagcaaa atgaaggcg atttatcac actttgaat ttctttaat atggacgtg acgaaaaaa tgcttgatt ggggagtgt tgggtcagc agtaaagac attccaatc gtacagaag gccctgtcg gatggttcc agtgcttgc ggcagctcc attccgtca tttttgaac gtgattaac accaatgag gatcctccg acgttcaat agaacgaat aaattcacc agagggttc cagaatctg attgattcg tacggagtt gcatcgtat cgtgaagct aaccctgcc ctttacacc atcatcact tttccgttt ctgttcgct gtgatgttc ggtgacgtt ggacacgca atgatcatg gccctattt ggcggttat ttggtaatt tccgagaag aaaatcatg gccaaacgg agtaataac gaaattttc aacattttc ttcgccggg agatacatc attctcctc atgggtctc ttctcaatg tacaccggt tttgtctac aacgacatt ttctcaaag tccatgaac ttgttcggg tccagttgg tttgtccaa ttcaacaaa gaacaagcc ttggagttg gatgaacgc gatctggac ccccgatat gactacact gggaccccc tatttcatc ggcatggac cccgcgtgg caattggcc aaaaacaag atcattttc ctaaattcg tacaaaatg aaactgtct attattttc ggggtcgtt catatgatt ttcggcgtg tgcgtcagc gtggtcaat ttcgtccat ttcaggaaa tactcaagc atcttcctg gaattcctc ccacagatc ctcctgctg tgtttcctc ttcttgtgg atggtggtg atgatgttc atgaaatgg attatctat tcggctgat ttgggcaaa cctgttgag ttgggtact tcgtgcgca cccaatgtc ctcatctac ttcatcaac atgatgctg ttcaaagcg acagagtcg ccggaaggt tgcaaggat tacatgttt gagggccaa cagaccgtg caacaaatc ttagttttc ttgtcattg gcgtgtatc ccggtgctg ttgctcgga aagccgctt tatatcaaa tgcacgagg aaaagtaag cctcatgtg cgaagtaac ggcgatgtt aaccaagga atggagttg ggggaatac cccgaagcg cagcaaaac gcagctgcg acgtcgcac gaagacgag gaggaggag gaaccgatg agtgagatt ttcatccat caggcaatt cacacaatt gagtacgtt ttgagtact atttcgcac actgcgtcc tatttgcgg ttgtgggcc ttgtcgtta gctcacgcg caactttct gaagtattg tggagcatg ttgttcagg atgggcttg acaaatagc tcgtatgtt ggggcgatt acggccttt gtgtttttc gcagcgtgg gcggctttt acgatagcc attttagtc acaatggaa gggttatca gcgtttttg cacactttg cgtttgcat tgggtcgaa tttatgagt aagttttat gccgggttg ggatatccg ttccaacca ttcagtttt aaagcaatc ctcgaggaa gaaaatgcg ccagaggaa taa
- >TC004949 RA
attgtgttg aacctgaag aaaacgtgc gtctggttg tagtgtgaa cgtacagtt tgtggtgaa atgactgta actttaaaa tcggcgctg agtgtcacg tgattgtta ttttgtttt tgttttatt tacgcagaa agtgaaacc gcctcaaaa tgggggaca tgttccgca gtgagcaaa tggtacttg ctcagctct tcatccaac ctgaggctg catatttcg ccatatcgg agctcggag aatccggaa tcgttcaat tccgagact tgaatgaaa acgtgaacg tgttccaac gaaaattcg taaacgagg tccgaagat gcgacgaaa tggagcgca agctgcgct acatcgagg ccgaggtga agaaagaca atgtggcga ttcccgacc agtctgagc tccccaaag ctccaaatc ccagggaga ttattgatc tggaagccc accttgaga aaaccgaag gcgatatca aggagttgt ccgaaagcg cggttaacc tgaagtcca attatctgg agctgatcg agttgaagc aagtgcttg agaaaactc aagctttct ttaacgagc aagacgaag ccaacggtt tagattcgg cccacaagg ctttgatca atgacgaaa gtcacaatg tgtccatta ggggccgtt tggggttcg ttgcggggg ttatcaacc gggagcgag tgccaggtt tcgagagaa tgctctgga ggatatcca gagggaacg tttttttga gacaggtcg agattgaaa aaccgctcg aagatccgg ccacgggga atcaactgt acaaaaccg ttttcgttg cgtttttcc aaggcgagc agttgaaaa caaggatta agaaagttt gtgctggat atcacgcct cgctttatg cgtgtccaa gctcgcttc aagaacgca acgagatgc ttaaaggtg tctgcacga ggttggaag atttgaatt tggttctaa accaaacgc aagaccacc ggcaaagag tgctagtga gtgtggcca aggaattgc aaaattgga gcgtgatgg ttagcaaaa tgaaggcga tttatcaca ctttgaatt tctttaata tggacgtga cgaaaaaat gcttgattg gggagtgtt gggtcagca gtaaagaca ttccaatcg tacagaagg ccctgtcgg atggttcca gtgcttgcg gcagctcca ttccgtcat ttttgaacg tgattaaca ccaatgagg atcctccga cgttcaata gaacgaata aattcacca gagggttcc agaatctga ttgattcgt acggagttg catcgtatc gtgaagcta accctgccc tttacacca tcatcactt ttccgtttc tgttcgctg tgatgttcg gtgacgttg gacacgcaa tgatcatgg ccctatttg gcggttatt tggtaattt ccgagaaga aaatcatgg ccaaacgga gtaataacg aaattttca acattttct tcgccggga gatacatca ttctcctca tgggtctct tctcaatgt acaccggtt ttgtctaca acgacattt tctcaaagt ccatgaact tgttcgggt ccagttggt ttgtccaat tcaacaaag aacaagcct tggagttgg atgaacgcg atctggacc cccgatatg actacactg ggaccccct atttcatcg gcatggacc ccgcgtggc aattggcca aaaacaaga tcattttcc taaattcgt acaaaatga aactgtcta ttattttcg gggtcgttc atatgattt tcggcgtgt gcgtcagcg tggtcaatt tcgtccatt tcaggaaat actcaagca tcttcctgg aattcctcc cacagatcc tcctgctgt gtttcctct tcttgtgga tggtggtga tgatgttca tgaaatgga ttatctatt cggctgatt tgggcaaac ctgttgagt tgggtactt cgtgcgcac ccaatgtcc tcatctact tcatcaaca tgatgctgt tcaaagcga cagagtcgc cggaaggtt gcaaggatt acatgtttg agggccaac agaccgtgc aacaaatct tagttttct tgtcattgg cgtgtatcc cggtgctgt tgctcggaa agccgcttt atatcaaat gcacgagga aaagtaagc ctcatgtgc gaagtaacg gcgatgtta accaaggaa tggagttgg gggaatacc ccgaagcgc agcaaaacg cagctgcga cgtcgcacg aagacgagg aggaggagg aaccgatga gtgagattt tcatccatc aggcaattc acacaattg agtacgttt tgagtacta tttcgcaca ctgcgtcct atttgcggt tgtgggcct tgtcgttag ctcacgcgc aactttctg aagtattgt ggagcatgt tgttcagga tgggcttga caaatagct cgtatgttg gggcgatta cggcctttg tgtttttcg cagcgtggg cggctttta cgatagcca ttttagtca caatggaag ggttatcag cgtttttgc acactttgc gtttgcatt gggtcgaat ttatgagta agttttatg ccgggttgg gatatccgt tccaaccat tcagtttta aagcaatcc tcgaggaag aaaatgcgc cagaggaat aacactttt atcagcttt ttagatagt gcacaaata gaatagcag caaataaaa atacttgtt aaaa
