Status
Please wait ...
Gene information
General information
TC004949 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4969373..4979486
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004949
    iBeetle screen iB_00788

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004949 RA
      MGDMFRSEQ MVLAQLFIQ PEAAYFAIS ELGESGIVQ 
      FRDLNENVN VFQRKFVNE VRRCDEMER KLRYIEAEV 
      KKDNVAIPD QSELPKAPN PREIIDLEA HLEKTEGDI 
      KELSESAVN LKSNYLELI ELKQVLEKT QAFFNEQDE 
      ANGLDSAHK ALINDESHN VSIRGRLGF VAGVINRER 
      VPGFERMLW RISRGNVFL RQVEIEKPL EDPATGNQL 
      YKTVFVAFF QGEQLKTRI KKVCAGYHA SLYACPSSL 
      QERNEMLKG VCTRLEDLN LVLNQTQDH RQRVLVSVA 
      KELQNWSVM VSKMKAIYH TLNFFNMDV TKKCLIGEC 
      WVSSKDIPI VQKALSDGS SACGSSIPS FLNVINTNE 
      DPPTFNRTN KFTRGFQNL IDSYGVASY REANPALYT 
      IITFPFLFA VMFGDVGHA MIMALFGGY LVISEKKIM 
      AKRSNNEIF NIFFAGRYI ILLMGLFSM YTGFVYNDI 
      FSKSMNLFG SSWFVQFNK EQALELDER DLDPRYDYT 
      GTPYFIGMD PAWQLAKNK IIFLNSYKM KLSIIFGVV 
      HMIFGVCVS VVNFVHFRK YSSIFLEFL PQILLLCFL 
      FLWMVVMMF MKWIIYSAD LGKPVELGT SCAPNVLIY 
      FINMMLFKA TESPEGCKD YMFEGQQTV QQILVFLSL 
      ACIPVLLLG KPLYIKCTR KSKPHVRSN GDVNQGMEL 
      GEYPEAQQN AAATSHEDE EEEEPMSEI FIHQAIHTI 
      EYVLSTISH TASYLRLWA LSLAHAQLS EVLWSMLFR 
      MGLTNSSYV GAITAFVFF AAWAAFTIA ILVTMEGLS 
      AFLHTLRLH WVEFMSKFY AGLGYPFQP FSFKAILEE 
      ENAPEE
    CDS sequence
    • >TC004949 RA
      atgggggac atgttccgc agtgagcaa atggtactt 
      gctcagctc ttcatccaa cctgaggct gcatatttc 
      gccatatcg gagctcgga gaatccgga atcgttcaa 
      ttccgagac ttgaatgaa aacgtgaac gtgttccaa 
      cgaaaattc gtaaacgag gtccgaaga tgcgacgaa 
      atggagcgc aagctgcgc tacatcgag gccgaggtg 
      aagaaagac aatgtggcg attcccgac cagtctgag 
      ctccccaaa gctccaaat cccagggag attattgat 
      ctggaagcc caccttgag aaaaccgaa ggcgatatc 
      aaggagttg tccgaaagc gcggttaac ctgaagtcc 
      aattatctg gagctgatc gagttgaag caagtgctt 
      gagaaaact caagctttc tttaacgag caagacgaa 
      gccaacggt ttagattcg gcccacaag gctttgatc 
      aatgacgaa agtcacaat gtgtccatt aggggccgt 
      ttggggttc gttgcgggg gttatcaac cgggagcga 
      gtgccaggt ttcgagaga atgctctgg aggatatcc 
      agagggaac gtttttttg agacaggtc gagattgaa 
      aaaccgctc gaagatccg gccacgggg aatcaactg 
      tacaaaacc gttttcgtt gcgtttttc caaggcgag 
      cagttgaaa acaaggatt aagaaagtt tgtgctgga 
      tatcacgcc tcgctttat gcgtgtcca agctcgctt 
      caagaacgc aacgagatg cttaaaggt gtctgcacg 
      aggttggaa gatttgaat ttggttcta aaccaaacg 
      caagaccac cggcaaaga gtgctagtg agtgtggcc 
      aaggaattg caaaattgg agcgtgatg gttagcaaa 
      atgaaggcg atttatcac actttgaat ttctttaat 
      atggacgtg acgaaaaaa tgcttgatt ggggagtgt 
      tgggtcagc agtaaagac attccaatc gtacagaag 
      gccctgtcg gatggttcc agtgcttgc ggcagctcc 
      attccgtca tttttgaac gtgattaac accaatgag 
      gatcctccg acgttcaat agaacgaat aaattcacc 
      agagggttc cagaatctg attgattcg tacggagtt 
      gcatcgtat cgtgaagct aaccctgcc ctttacacc 
      atcatcact tttccgttt ctgttcgct gtgatgttc 
      ggtgacgtt ggacacgca atgatcatg gccctattt 
      ggcggttat ttggtaatt tccgagaag aaaatcatg 
      gccaaacgg agtaataac gaaattttc aacattttc 
      ttcgccggg agatacatc attctcctc atgggtctc 
      ttctcaatg tacaccggt tttgtctac aacgacatt 
      ttctcaaag tccatgaac ttgttcggg tccagttgg 
      tttgtccaa ttcaacaaa gaacaagcc ttggagttg 
      gatgaacgc gatctggac ccccgatat gactacact 
      gggaccccc tatttcatc ggcatggac cccgcgtgg 
      caattggcc aaaaacaag atcattttc ctaaattcg 
      tacaaaatg aaactgtct attattttc ggggtcgtt 
      catatgatt ttcggcgtg tgcgtcagc gtggtcaat 
      ttcgtccat ttcaggaaa tactcaagc atcttcctg 
      gaattcctc ccacagatc ctcctgctg tgtttcctc 
      ttcttgtgg atggtggtg atgatgttc atgaaatgg 
      attatctat tcggctgat ttgggcaaa cctgttgag 
      ttgggtact tcgtgcgca cccaatgtc ctcatctac 
      ttcatcaac atgatgctg ttcaaagcg acagagtcg 
      ccggaaggt tgcaaggat tacatgttt gagggccaa 
      cagaccgtg caacaaatc ttagttttc ttgtcattg 
      gcgtgtatc ccggtgctg ttgctcgga aagccgctt 
      tatatcaaa tgcacgagg aaaagtaag cctcatgtg 
      cgaagtaac ggcgatgtt aaccaagga atggagttg 
      ggggaatac cccgaagcg cagcaaaac gcagctgcg 
      acgtcgcac gaagacgag gaggaggag gaaccgatg 
      agtgagatt ttcatccat caggcaatt cacacaatt 
      gagtacgtt ttgagtact atttcgcac actgcgtcc 
      tatttgcgg ttgtgggcc ttgtcgtta gctcacgcg 
      caactttct gaagtattg tggagcatg ttgttcagg 
      atgggcttg acaaatagc tcgtatgtt ggggcgatt 
      acggccttt gtgtttttc gcagcgtgg gcggctttt 
      acgatagcc attttagtc acaatggaa gggttatca 
      gcgtttttg cacactttg cgtttgcat tgggtcgaa 
      tttatgagt aagttttat gccgggttg ggatatccg 
      ttccaacca ttcagtttt aaagcaatc ctcgaggaa 
      gaaaatgcg ccagaggaa taa
    mRNA sequence
    • >TC004949 RA
      attgtgttg aacctgaag aaaacgtgc gtctggttg 
      tagtgtgaa cgtacagtt tgtggtgaa atgactgta 
      actttaaaa tcggcgctg agtgtcacg tgattgtta 
      ttttgtttt tgttttatt tacgcagaa agtgaaacc 
      gcctcaaaa tgggggaca tgttccgca gtgagcaaa 
      tggtacttg ctcagctct tcatccaac ctgaggctg 
      catatttcg ccatatcgg agctcggag aatccggaa 
      tcgttcaat tccgagact tgaatgaaa acgtgaacg 
      tgttccaac gaaaattcg taaacgagg tccgaagat 
      gcgacgaaa tggagcgca agctgcgct acatcgagg 
      ccgaggtga agaaagaca atgtggcga ttcccgacc 
      agtctgagc tccccaaag ctccaaatc ccagggaga 
      ttattgatc tggaagccc accttgaga aaaccgaag 
      gcgatatca aggagttgt ccgaaagcg cggttaacc 
      tgaagtcca attatctgg agctgatcg agttgaagc 
      aagtgcttg agaaaactc aagctttct ttaacgagc 
      aagacgaag ccaacggtt tagattcgg cccacaagg 
      ctttgatca atgacgaaa gtcacaatg tgtccatta 
      ggggccgtt tggggttcg ttgcggggg ttatcaacc 
      gggagcgag tgccaggtt tcgagagaa tgctctgga 
      ggatatcca gagggaacg tttttttga gacaggtcg 
      agattgaaa aaccgctcg aagatccgg ccacgggga 
      atcaactgt acaaaaccg ttttcgttg cgtttttcc 
      aaggcgagc agttgaaaa caaggatta agaaagttt 
      gtgctggat atcacgcct cgctttatg cgtgtccaa 
      gctcgcttc aagaacgca acgagatgc ttaaaggtg 
      tctgcacga ggttggaag atttgaatt tggttctaa 
      accaaacgc aagaccacc ggcaaagag tgctagtga 
      gtgtggcca aggaattgc aaaattgga gcgtgatgg 
      ttagcaaaa tgaaggcga tttatcaca ctttgaatt 
      tctttaata tggacgtga cgaaaaaat gcttgattg 
      gggagtgtt gggtcagca gtaaagaca ttccaatcg 
      tacagaagg ccctgtcgg atggttcca gtgcttgcg 
      gcagctcca ttccgtcat ttttgaacg tgattaaca 
      ccaatgagg atcctccga cgttcaata gaacgaata 
      aattcacca gagggttcc agaatctga ttgattcgt 
      acggagttg catcgtatc gtgaagcta accctgccc 
      tttacacca tcatcactt ttccgtttc tgttcgctg 
      tgatgttcg gtgacgttg gacacgcaa tgatcatgg 
      ccctatttg gcggttatt tggtaattt ccgagaaga 
      aaatcatgg ccaaacgga gtaataacg aaattttca 
      acattttct tcgccggga gatacatca ttctcctca 
      tgggtctct tctcaatgt acaccggtt ttgtctaca 
      acgacattt tctcaaagt ccatgaact tgttcgggt 
      ccagttggt ttgtccaat tcaacaaag aacaagcct 
      tggagttgg atgaacgcg atctggacc cccgatatg 
      actacactg ggaccccct atttcatcg gcatggacc 
      ccgcgtggc aattggcca aaaacaaga tcattttcc 
      taaattcgt acaaaatga aactgtcta ttattttcg 
      gggtcgttc atatgattt tcggcgtgt gcgtcagcg 
      tggtcaatt tcgtccatt tcaggaaat actcaagca 
      tcttcctgg aattcctcc cacagatcc tcctgctgt 
      gtttcctct tcttgtgga tggtggtga tgatgttca 
      tgaaatgga ttatctatt cggctgatt tgggcaaac 
      ctgttgagt tgggtactt cgtgcgcac ccaatgtcc 
      tcatctact tcatcaaca tgatgctgt tcaaagcga 
      cagagtcgc cggaaggtt gcaaggatt acatgtttg 
      agggccaac agaccgtgc aacaaatct tagttttct 
      tgtcattgg cgtgtatcc cggtgctgt tgctcggaa 
      agccgcttt atatcaaat gcacgagga aaagtaagc 
      ctcatgtgc gaagtaacg gcgatgtta accaaggaa 
      tggagttgg gggaatacc ccgaagcgc agcaaaacg 
      cagctgcga cgtcgcacg aagacgagg aggaggagg 
      aaccgatga gtgagattt tcatccatc aggcaattc 
      acacaattg agtacgttt tgagtacta tttcgcaca 
      ctgcgtcct atttgcggt tgtgggcct tgtcgttag 
      ctcacgcgc aactttctg aagtattgt ggagcatgt 
      tgttcagga tgggcttga caaatagct cgtatgttg 
      gggcgatta cggcctttg tgtttttcg cagcgtggg 
      cggctttta cgatagcca ttttagtca caatggaag 
      ggttatcag cgtttttgc acactttgc gtttgcatt 
      gggtcgaat ttatgagta agttttatg ccgggttgg 
      gatatccgt tccaaccat tcagtttta aagcaatcc 
      tcgaggaag aaaatgcgc cagaggaat aacactttt 
      atcagcttt ttagatagt gcacaaata gaatagcag 
      caaataaaa atacttgtt aaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY