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Gene information
General information
TC004939 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4845499..4846579
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004939
    iBeetle screen iB_00786

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    remarks: later than schedule, pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    Pupal morphology
    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004939 RA
      MEVLNLKEG ETVLAVCEE GTKSLSDTT LNTITNLKS 
PNELSGLTE NSNDSILVE LPQEQLTES LTKQLFKLC 
KPGGQLCIH KVKDFDTLE LSLKTSGFT NVRNENNWL 
LSNKPKYEI GSSAKLNLK KPVWKLDDA DEGETIDPD 
NLLDDEDLK KPDPSSLRV CGTTGKRKA CKDCSCGLA 
DELAQEAKA GQLVDTTDA PKSSCGNCY LGDAFRCAS 
CPYLGMPAF KPGEKIQLS DSLLKADI
    CDS sequence
    • >TC004939 RA
      atggaagtg ttaaactta aaagagggt gaaacggta 
ctagcggta tgtgaagag ggtacaaaa tcactctca 
gacaccacc cttaacact atcacaaac ctcaaatca 
ccaaatgag ttatcagga ctcaccgag aactccaac 
gactcgatt ctggtcgaa ctgccccag gaacaacta 
acagagtcc ttaaccaaa caattgttc aagttgtgc 
aaacctggg ggccaactg tgcatccac aaggttaaa 
gactttgac actcttgag ctcagcttg aaaactagt 
gggtttaca aacgtccgt aatgaaaac aactggctg 
ctttcaaat aaaccaaaa tacgaaatt ggctcttct 
gccaaactt aaccttaaa aaaccggta tggaaattg 
gatgatgcc gatgagggt gaaactata gaccccgat 
aatttgctc gatgatgag gatttgaag aaaccagac 
ccttcgtca cttagagtt tgtggtacc actggtaaa 
cgcaaggct tgcaaagac tgctcgtgt ggccttgcg 
gacgaactc gcacaggag gcaaaggcc gggcagctt 
gtggacaca actgacgcc ccaaaatcg tcctgtggc 
aactgctac ttgggggac gccttccgg tgcgcgagt 
tgcccttat ctgggaatg ccggccttc aagccgggc 
gaaaagatc caactcagt gacagtctt ttgaaggca 
gatatttaa
    mRNA sequence
    • >TC004939 RA
      gcgcacaaa agccatcaa aatagttca aattgcagg 
agccaagtg aattttcca tttactaaa ttacacgat 
ggaagtgtt aaacttaaa agagggtga aacggtact 
agcggtatg tgaagaggg tacaaaatc actctcaga 
caccaccct taacactat cacaaacct caaatcacc 
aaatgagtt atcaggact caccgagaa ctccaacga 
ctcgattct ggtcgaact gccccagga acaactaac 
agagtcctt aaccaaaca attgttcaa gttgtgcaa 
acctggggg ccaactgtg catccacaa ggttaaaga 
ctttgacac tcttgagct cagcttgaa aactagtgg 
gtttacaaa cgtccgtaa tgaaaacaa ctggctgct 
ttcaaataa accaaaata cgaaattgg ctcttctgc 
caaacttaa ccttaaaaa accggtatg gaaattgga 
tgatgccga tgagggtga aactataga ccccgataa 
tttgctcga tgatgagga tttgaagaa accagaccc 
ttcgtcact tagagtttg tggtaccac tggtaaacg 
caaggcttg caaagactg ctcgtgtgg ccttgcgga 
cgaactcgc acaggaggc aaaggccgg gcagcttgt 
ggacacaac tgacgcccc aaaatcgtc ctgtggcaa 
ctgctactt gggggacgc cttccggtg cgcgagttg 
cccttatct gggaatgcc ggccttcaa gccgggcga 
aaagatcca actcagtga cagtctttt gaaggcaga 
tatttaatt gattccaca tccagcata tgtataaat 
attctatta ataaaagcg ttaaataaa t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY