Status
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Gene information
General information
TC032021
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004927,TC004926
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid6: 2639795..2646743
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032021
    iBeetle screen iB_00784
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection



    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Pupal morphology
    wings orientation irregular - Number of animals showing the phenotype: 1

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032021 RA
      MSFYILNNS RKICHHRTI IYKNWASNF TNQGLRKPP 
      NFISARNFS RKERTSDRF EWKGILTRF VPVGICLIA 
      VMQWRSYRK KSSERLANK WEIDCYCML PLRTVSRWW 
      GWLADKEIP EFLRPVVYG LYANTFGVK LSEALHEDL 
      RSYPSLADF FARPLKSGI RQVDHESDL VSPCDGTVL 
      HFGTVHTGE IEQVKGVTY SLEKFLGEN TWNNNNNVK 
      DYRTSLLHN AHTLYQCVI YLAPGDYHR FHSAANWKP 
      THRRHFHGE LLSVSPRIA KWVPGLFCL NERAVYLGS 
      WDHGFFSYT AVGATNVGT VKVYCDKTL HTNHPKKSD 
      RCKDLCLGN GTYFKKGDP FGEFRMGST IVLVFEAPV 
      NFQFTILPG DRVQMGQGL GRVGQHKFI MNLKQVLLL 
      KNDFLTVFG LLAFVIKAE SGPNNLHVH DGATTAEII 
      AGDIFFEIV DPLELEYTY RIRPAKDFG APFNESFYI 
      KHVPLVPIQ PKFGCTPPE NIEDIEGNV ALIERGECS 
      FKMKAKIAE KAGAQAVII TDVSKPTEE YFIEMIDDD 
      SSDEVHIPA AFLMGKNGI MITKTLERL KRSYAIINL 
      PVNLTFTPV HEMNQPPWL GW
    CDS sequence
    • >TC032021 RA
      atgtctttt tacatatta aataattcc cgcaaaata 
      tgtcaccac cggaccatc atctacaaa aactgggcc 
      tcaaatttt acaaatcag ggtttacga aaaccccct 
      aattttatc tctgccaga aacttttca cgcaaggaa 
      cgaacttct gatcggttt gaatggaag ggaattttg 
      actagattc gttccagta ggaatatgt cttattgct 
      gtgatgcaa tggcgttct taccggaaa aaatcgtca 
      gaacgcttg gccaacaaa tgggaaatt gactgttac 
      tgtatgttg ccgttgagg acggtttcg cggtggtgg 
      ggctggctt gcagacaaa gaaataccg gagttttta 
      cgacctgta gtctatggt ttgtatgct aacactttc 
      ggcgttaaa ttgtcggaa gctttacat gaggattta 
      aggtcttat ccaagtcta gcggacttc tttgcgagg 
      cctttgaaa agcggaatt cggcaagtt gaccacgag 
      agtgactta gtttcacct tgtgacggc actgtgctg 
      catttcggc acggtccac actggcgag atcgaacaa 
      gtgaaaggt gtaacctac tcgcttgaa aaattcctg 
      ggtgaaaac acttggaat aataacaac aacgttaaa 
      gattacaga acgtcgctg cttcacaac gcccacaca 
      ctgtaccag tgtgttatt tatttggcc ccgggggac 
      taccacagg ttccattcg gctgcaaat tggaagcca 
      acccaccgg cgccatttc cacggagaa ctcctgtcg 
      gtgagtccc cggatcgcc aaatgggtt cctgggttg 
      ttctgtctg aacgaacgc gcagtgtat ttgggttca 
      tgggaccac gggtttttc tcatacacc gcagttggt 
      gccacaaat gtggggaca gtgaaagtc tactgcgac 
      aaaaccctc cacacaaat cacccgaaa aaatcagat 
      cgttgcaaa gatctgtgt ttgggtaat gggacgtat 
      tttaagaaa ggggatccg tttggggag tttcgaatg 
      ggttctact atcgtctta gtgtttgaa gcacccgtt 
      aattttcaa ttcacgatc ttaccgggt gatagagta 
      caaatgggt caaggctta ggtcgagtt gggcaacat 
      aaatttatt atgaacctc aagcaagtc ttgttgcta 
      aaaaacgat tttttaaca gtgttcggt ttgctggct 
      tttgttata aaggcggaa agtggtcca aataattta 
      cacgttcat gacggtgcc acaacggct gaaataata 
      gctggtgat atatttttt gaaatagtt gacccctta 
      gagctagaa tatacgtac aggataagg cctgccaaa 
      gattttgga gcacctttt aacgaatcg ttttatatt 
      aaacatgtc ccgcttgtg cctattcaa ccaaagttt 
      ggttgtaca ccccctgag aacatcgag gatattgaa 
      gggaacgtt gctttaatt gagagaggg gaatgttcg 
      ttcaaaatg aaggcaaaa attgcggaa aaagccggt 
      gctcaagcc gtaattata acagatgtg tccaagccg 
      actgaagaa tactttatt gagatgatt gatgatgac 
      tcatctgat gaagttcac atacctgcg gcttttctc 
      atgggcaaa aacggtatt atgatcaca aaaacgctg 
      gaaaggtta aaaagatcg tatgcaata atcaattta 
      cctgtaaat ttaaccttc acaccagta catgaaatg 
      aatcaacca ccgtggtta ggatggtaa
    mRNA sequence
    • >TC032021 RA
      tgcgcaaat gaccctatg tcaaaaaag tcaaacgta 
      taacctcac ttttggtcc aatgaaaat gtgtctatt 
      ggtttaatt tgtgttttc aatttacaa gaaaataca 
      tccataatg tctttttac atattaaat aattcccgc 
      aaaatatgt caccaccgg accatcatc tacaaaaac 
      tgggcctca aattttaca aatcagggt ttacgaaaa 
      ccccctaat tttatctct gccagaaac ttttcacgc 
      aaggaacga acttctgat cggtttgaa tggaaggga 
      attttgact agattcgtt ccagtagga atatgtctt 
      attgctgtg atgcaatgg cgttcttac cggaaaaaa 
      tcgtcagaa cgcttggcc aacaaatgg gaaattgac 
      tgttactgt atgttgccg ttgaggacg gtttcgcgg 
      tggtggggc tggcttgca gacaaagaa ataccggag 
      tttttacga cctgtagtc tatggtttg tatgctaac 
      actttcggc gttaaattg tcggaagct ttacatgag 
      gatttaagg tcttatcca agtctagcg gacttcttt 
      gcgaggcct ttgaaaagc ggaattcgg caagttgac 
      cacgagagt gacttagtt tcaccttgt gacggcact 
      gtgctgcat ttcggcacg gtccacact ggcgagatc 
      gaacaagtg aaaggtgta acctactcg cttgaaaaa 
      ttcctgggt gaaaacact tggaataat aacaacaac 
      gttaaagat tacagaacg tcgctgctt cacaacgcc 
      cacacactg taccagtgt gttatttat ttggccccg 
      ggggactac cacaggttc cattcggct gcaaattgg 
      aagccaacc caccggcgc catttccac ggagaactc 
      ctgtcggtg agtccccgg atcgccaaa tgggttcct 
      gggttgttc tgtctgaac gaacgcgca gtgtatttg 
      ggttcatgg gaccacggg tttttctca tacaccgca 
      gttggtgcc acaaatgtg gggacagtg aaagtctac 
      tgcgacaaa accctccac acaaatcac ccgaaaaaa 
      tcagatcgt tgcaaagat ctgtgtttg ggtaatggg 
      acgtatttt aagaaaggg gatccgttt ggggagttt 
      cgaatgggt tctactatc gtcttagtg tttgaagca 
      cccgttaat tttcaattc acgatctta ccgggtgat 
      agagtacaa atgggtcaa ggcttaggt cgagttggg 
      caacataaa tttattatg aacctcaag caagtcttg 
      ttgctaaaa aacgatttt ttaacagtg ttcggtttg 
      ctggctttt gttataaag gcggaaagt ggtccaaat 
      aatttacac gttcatgac ggtgccaca acggctgaa 
      ataatagct ggtgatata ttttttgaa atagttgac 
      cccttagag ctagaatat acgtacagg ataaggcct 
      gccaaagat tttggagca ccttttaac gaatcgttt 
      tatattaaa catgtcccg cttgtgcct attcaacca 
      aagtttggt tgtacaccc cctgagaac atcgaggat 
      attgaaggg aacgttgct ttaattgag agaggggaa 
      tgttcgttc aaaatgaag gcaaaaatt gcggaaaaa 
      gccggtgct caagccgta attataaca gatgtgtcc 
      aagccgact gaagaatac tttattgag atgattgat 
      gatgactca tctgatgaa gttcacata cctgcggct 
      tttctcatg ggcaaaaac ggtattatg atcacaaaa 
      acgctggaa aggttaaaa agatcgtat gcaataatc 
      aatttacct gtaaattta accttcaca ccagtacat 
      gaaatgaat caaccaccg tggttagga tggtaatta 
      gtgaatgaa acacaaatc agggtcaag ttcctcagt 
      tgttaaccc cagttggac actgtactt atgtttatt 
      tattgccaa acaagttat taactggtg tggggttgt 
      attgtttaa atggccacg tctgtgcca tgtgtgaaa 
      aattgctca cctctagtc aactgttag caataattt 
      tttaagaat ttactgtct taattgttt gtacatatc 
      acttgtatt tattgaata aattttaag gtgttct