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Gene information
General information
TC004901 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid6: 2038086..2045420
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004901
    iBeetle screen iB_00782
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax segment sometimes number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004901 RA
      MYSTTVLLV LIGVGIVKS RSLTSIETI PRGCDWQMQ 
      PVENEAREE PVLSCRIRT INSAGPLLG NLSQTQIDR 
      VAVLRLECS DMLFFESSL EAGSKHGSF LTGLRWLKE 
      LKLEFCKMR NIPAGVLKP LRQLRAFSL RSHNSDWSS 
      MTLELHPES FLGLSELRS LDLGDNNIW STPPDLFCP 
      LFSLTHLNL TMNKLQNLS ALGFSDWGN GPLAPGRAC 
      VSGLEILDL SHNDIIALP DNGLSGLRS LEELHLQDN 
      AVSTLGDRA FVGLTSLHT LNLSSNCLV ALPPELFQS 
      SRDLKHLYL HNNSLSVLA PGLLEGLDQ LTVLDLSRN 
      ELSSHWVNR DTFAGLVRL VVLNLAHNQ LTRIDANLF 
      HDLYTLQIL NLENNQIEF IADGAFSEL KNLHALTLT 
      HNFITRIEA YHFSGMYAL NQLLLDSNK IEYIHPQTF 
      ENVTNLQDL GLNGNMLGG VPVGIGKLR FLKTLDLGK 
      NHIEIVNNS SFEGLDSLY GLRLVDNHI VNISRDAFS 
      TLPSLQVLN LASNKIKYV EQSAFASNP TLKAIRLDA 
      NELSDISGV FTNLQSLVW LNVSSNKLL WFDFSHLPS 
      SLEWLDMHD NKITELGNY YDVKNTLKI KMLDASFNL 
      LTDVHDNCI PDSVETLFL NNNKITNVH PNTFVKKIH 
      LQKVVLYGN DIKYLDIAA LSLSTVSDD KDLPQFYIG 
      GNPFYCDCK MEWLLRINH LSNLRQYPQ VLDLDTVTC 
      ELAHARGSP PQPLLSLKP SQFLCPYKS HCFALCHCC 
      EFDACDCEM TCPTNCTCF HDHTWSSNV VDCSNAGYT 
      NVPEKIPMD ATEIYLDGN NLGELGSHV FIGKKKLEV 
      LFLNNSNVN AVHNRTFNG VKSLKVLHM ENNNLEELR 
      GFEFDQLEK LNELYLDHN KIAFVSNQT FKNMHELEV 
      LKLDENKIV DFIPHWENV APRVTLDGN KWSCDCNSL 
      GRLEIWIRE TGGDPSKLF CLDRDDSET VADVITRCD 
      NKDNAIATS VIQREVLKN NPLLGGSYV PLLAATLVA 
      VIVFCLLAA VVFVFRQDV RLWAHSKYG IRIFQDAAT 
      AHDNKDERD RLYDCYMVY SHKDEELAA RIIAPELEQ 
      LGHTLCLHN RDLALHGGA SYLADAMVG ASDASRRVI 
      FLVSPALIC NEWSRPEFR AALQTALRT AHRHKLVCV 
      LSGDPLEPM DPELRALLR ACTVTRWGE RRFWEKLRY 
      AMPDVANSN RSRRGKKHH QPEIRCKVS ARYTAAPTA 
      PDSWYKYSA MPGVHAALT PTPTQSTYV SGESARSTE 
      DEGSSASSQ HYGSEQLNH SYVSIDGRP PPQYTQYPI 
      CRPEIAHHL YSTIPDTQP QSRTYFV
    CDS sequence
    • >TC004901 RA
      atgtattca acaacagta ctattagtg ttgatcggt 
      gtcggaatt gtgaaatcg cgctctcta accagcatc 
      gagacaata ccacgagga tgcgactgg cagatgcaa 
      ccggtggag aacgaagcc agagaggag ccagtgctc 
      tcctgcagg atacggact attaacagt gccggaccc 
      ttgctgggc aacctcagc cagacccag attgaccgc 
      gttgcagtt ctaagactc gaatgtagc gatatgttg 
      tttttcgaa agttcgttg gaggcgggc agcaagcac 
      ggaagtttc ctcaccggc ttgcgttgg ttgaaagaa 
      ctcaagttg gaattttgc aaaatgagg aacatcccg 
      gccggtgtt ttgaaaccg ctgcgccaa ttacgagcg 
      ttctccctc cgttcacac aattccgac tggtcttcg 
      atgactctg gaattgcac cctgagagc tttctcggc 
      ttgagcgag ttgcgcagc ctggatttg ggcgacaac 
      aacatctgg agtacgccg ccggatttg ttttgcccg 
      ctcttcagt ctcacgcat ttaaatttg acgatgaat 
      aaactgcag aatttgtcc gcgcttggc ttttccgac 
      tggggaaat gggcccttg gcgccgggg agggcctgc 
      gtcagcgga ctggaaatc ctcgatctg tcgcacaac 
      gacatcatt gcccttcct gacaacgga ttatccgga 
      ctgcgatcg ttggaagaa ctgcacttg caagataac 
      gccgtttcg acgttaggt gatcgcgcg ttcgtcggt 
      ttgacaagt ttgcacacg ctcaactta tccagcaat 
      tgtctcgtc gctcttccg ccagagttg tttcagtcg 
      tccagagac ctcaaacat ctctatctc cacaacaat 
      tcgctgagc gtgttagcg ccgggactt cttgaaggg 
      ttggatcag ttgacagtg cttgacttg tcgcggaac 
      gagctgtcg agccattgg gtcaataga gacactttc 
      gccggtctc gtgcgactt gttgtgctc aatttggcc 
      cacaatcaa ttaacgcgc atcgatgcc aacttgttc 
      catgatttg tacacctta caaatactc aatcttgag 
      aacaatcag atcgagttt atcgccgat ggggctttt 
      tccgaactg aagaaccta catgctctc accctaacc 
      cataatttt ataacgaga atagaggcc taccatttc 
      tccggtatg tatgccctc aatcaacta ctgctcgac 
      tcaaataaa atcgaatac attcatcca caaaccttc 
      gagaacgtg actaactta caagacttg ggtctgaac 
      gggaatatg ctggggggc gtccctgtc ggtattggg 
      aaattgaga ttcctcaaa acactagat ttaggcaaa 
      aatcatatc gaaattgtt aataattcg tcgtttgag 
      ggactggat tcgttgtac ggacttaga cttgtcgat 
      aatcatatt gtaaacatc tcacgagat gctttctcc 
      actttacct tcattacaa gttttgaac ttggcatcg 
      aataaaatt aaatacgtc gaacagagt gcttttgct 
      agtaatccg accttgaaa gccattaga ctggacgcc 
      aacgagtta tccgatata agtggcgtg ttcaccaat 
      ttacagagc ttggtttgg ttgaacgtt tcatcgaat 
      aagctgctc tggttcgac ttcagccat ttgccctcg 
      agcttagag tggcttgac atgcacgat aataaaata 
      accgaacta ggaaattat tatgacgta aaaaacacg 
      ttgaaaatt aaaatgcta gatgctagc tttaattta 
      ttgaccgat gtgcatgat aattgcatt ccggacagc 
      gtcgagacc ctcttcctc aacaacaac aaaattaca 
      aacgtccat ccgaacacc ttcgtcaaa aaaatccac 
      ctccaaaaa gtggttttg tacggcaat gacatcaaa 
      tacctcgat atagccgcg ctgagtctc agtacggtc 
      tccgacgat aaagacttg ccccagttt tacatcgga 
      ggcaaccct ttctactgc gactgcaaa atggagtgg 
      ctattgagg ataaaccac ttgagcaat ttgcgccaa 
      tacccccaa gtgctggat ttggatacc gtaacgtgc 
      gaactggct catgccagg gggtcgcca ccccagcca 
      ctgctcagt ctgaaaccg tcgcaattt ttgtgccct 
      tacaagagt cactgcttc gctttgtgt cactgttgc 
      gagtttgac gcttgcgat tgcgaaatg acgtgtcca 
      acaaactgc acgtgcttt cacgaccac acgtggtcc 
      agcaatgtg gtcgattgc agcaatgcg ggctacaca 
      aacgtcccc gagaagata ccgatggac gccactgaa 
      atatacctg gacggcaat aacttgggt gagctcggc 
      agtcacgta ttcatcggc aagaaaaaa ctggaagtc 
      ttgttttta aacaacagc aacgtcaac gccgtgcat 
      aacaggaca ttcaacggt gtcaaatcg ctcaaagtc 
      ctccacatg gaaaacaac aatttggaa gaactgcgc 
      ggcttcgag ttcgaccaa ctggaaaag ctcaacgaa 
      ttgtatctg gaccacaac aagatcgcg ttcgtcagc 
      aatcagacg ttcaaaaac atgcacgag ctggaggtt 
      ttgaagctg gacgagaat aaaatcgtc gactttatc 
      cctcattgg gaaaatgtg gctccgcgc gtgactctc 
      gacggcaac aagtggtcg tgcgactgc aattcgtta 
      ggtcgtctt gagatctgg atccgggag accggaggc 
      gacccttcc aaattattt tgtctggat cgggacgac 
      tccgaaacg gtggccgat gtgatcacc cgctgtgat 
      aacaaagac aatgccatt gccacgtcg gtcattcaa 
      cgagaagtg ctcaaaaac aacccgctt ttgggtggc 
      agttacgtg ccgcttttg gccgccacg ctcgtcgct 
      gtcattgtt ttctgtttg ctggctgct gtcgttttc 
      gtattccgg caagacgtt cgcttgtgg gcccactcg 
      aagtacggc attcgaatt tttcaagac gccgccact 
      gcgcacgat aataaagac gaacgtgat cgcctctac 
      gactgctac atggtatac agccacaag gatgaagaa 
      ctggccgcc aggataatt gccccagaa cttgaacaa 
      ctcggacac accctatgc cttcacaac agagacttg 
      gcgttgcac ggcggcgcc tcgtatctc gccgacgcc 
      atggtgggt gcctcggac gcgtcccga cgcgtcatc 
      ttcctggtc tctcctgcc ctcatctgc aacgagtgg 
      tctcgtccc gagttccgc gccgcgctg cagacggcc 
      cttcgcacg gcccaccgt cacaaacta gtgtgtgtt 
      ttgagcggt gaccctctg gagcccatg gaccccgag 
      ctgcgggcc ttgctccgg gcctgcaca gtgacgaga 
      tggggggag cgccgcttc tgggagaag ctgcgttat 
      gccatgccc gatgtggcc aacagcaac cggtcgcgc 
      cgcggcaag aaacaccac caaccggaa atccggtgt 
      aaggtcagt gcgagatac actgcggcg ccgacggcc 
      cccgactcc tggtacaag tactcggcg atgccgggg 
      gtgcacgcc gcgctgact ccaaccccc acgcagagc 
      acttacgtc tcgggggag tcggcccgg agcaccgag 
      gacgaaggg tccagtgcg agcagccag cattatggc 
      tccgagcag ttgaaccac agctacgtg tccatcgac 
      ggaaggcct ccgccgcag tacacacag tatccgata 
      tgtaggcca gaaattgcc caccatctc tattcgacc 
      attccggat acgcaacca caatctagg acttacttt 
      gtgtga
    mRNA sequence
    • >TC004901 RA
      tttcagttc cgactacgt cgttggaga gggtggatg 
      cgttcgctc tccggtaga tcgtcgggt agtcgtgcc 
      gttaccagt gatgattta acgaaaaac tgattcaag 
      tgtcttgcc ataatgtat tcaacaaca gtactatta 
      gtgttgatc ggtgtcgga attgtgaaa tcgcgctct 
      ctaaccagc atcgagaca ataccacga ggatgcgac 
      tggcagatg caaccggtg gagaacgaa gccagagag 
      gagccagtg ctctcctgc aggatacgg actattaac 
      agtgccgga cccttgctg ggcaacctc agccagacc 
      cagattgac cgcgttgca gttctaaga ctcgaatgt 
      agcgatatg ttgtttttc gaaagttcg ttggaggcg 
      ggcagcaag cacggaagt ttcctcacc ggcttgcgt 
      tggttgaaa gaactcaag ttggaattt tgcaaaatg 
      aggaacatc ccggccggt gttttgaaa ccgctgcgc 
      caattacga gcgttctcc ctccgttca cacaattcc 
      gactggtct tcgatgact ctggaattg caccctgag 
      agctttctc ggcttgagc gagttgcgc agcctggat 
      ttgggcgac aacaacatc tggagtacg ccgccggat 
      ttgttttgc ccgctcttc agtctcacg catttaaat 
      ttgacgatg aataaactg cagaatttg tccgcgctt 
      ggcttttcc gactgggga aatgggccc ttggcgccg 
      gggagggcc tgcgtcagc ggactggaa atcctcgat 
      ctgtcgcac aacgacatc attgccctt cctgacaac 
      ggattatcc ggactgcga tcgttggaa gaactgcac 
      ttgcaagat aacgccgtt tcgacgtta ggtgatcgc 
      gcgttcgtc ggtttgaca agtttgcac acgctcaac 
      ttatccagc aattgtctc gtcgctctt ccgccagag 
      ttgtttcag tcgtccaga gacctcaaa catctctat 
      ctccacaac aattcgctg agcgtgtta gcgccggga 
      cttcttgaa gggttggat cagttgaca gtgcttgac 
      ttgtcgcgg aacgagctg tcgagccat tgggtcaat 
      agagacact ttcgccggt ctcgtgcga cttgttgtg 
      ctcaatttg gcccacaat caattaacg cgcatcgat 
      gccaacttg ttccatgat ttgtacacc ttacaaata 
      ctcaatctt gagaacaat cagatcgag tttatcgcc 
      gatggggct ttttccgaa ctgaagaac ctacatgct 
      ctcacccta acccataat tttataacg agaatagag 
      gcctaccat ttctccggt atgtatgcc ctcaatcaa 
      ctactgctc gactcaaat aaaatcgaa tacattcat 
      ccacaaacc ttcgagaac gtgactaac ttacaagac 
      ttgggtctg aacgggaat atgctgggg ggcgtccct 
      gtcggtatt gggaaattg agattcctc aaaacacta 
      gatttaggc aaaaatcat atcgaaatt gttaataat 
      tcgtcgttt gagggactg gattcgttg tacggactt 
      agacttgtc gataatcat attgtaaac atctcacga 
      gatgctttc tccacttta ccttcatta caagttttg 
      aacttggca tcgaataaa attaaatac gtcgaacag 
      agtgctttt gctagtaat ccgaccttg aaagccatt 
      agactggac gccaacgag ttatccgat ataagtggc 
      gtgttcacc aatttacag agcttggtt tggttgaac 
      gtttcatcg aataagctg ctctggttc gacttcagc 
      catttgccc tcgagctta gagtggctt gacatgcac 
      gataataaa ataaccgaa ctaggaaat tattatgac 
      gtaaaaaac acgttgaaa attaaaatg ctagatgct 
      agctttaat ttattgacc gatgtgcat gataattgc 
      attccggac agcgtcgag accctcttc ctcaacaac 
      aacaaaatt acaaacgtc catccgaac accttcgtc 
      aaaaaaatc cacctccaa aaagtggtt ttgtacggc 
      aatgacatc aaatacctc gatatagcc gcgctgagt 
      ctcagtacg gtctccgac gataaagac ttgccccag 
      ttttacatc ggaggcaac cctttctac tgcgactgc 
      aaaatggag tggctattg aggataaac cacttgagc 
      aatttgcgc caatacccc caagtgctg gatttggat 
      accgtaacg tgcgaactg gctcatgcc agggggtcg 
      ccaccccag ccactgctc agtctgaaa ccgtcgcaa 
      tttttgtgc ccttacaag agtcactgc ttcgctttg 
      tgtcactgt tgcgagttt gacgcttgc gattgcgaa 
      atgacgtgt ccaacaaac tgcacgtgc tttcacgac 
      cacacgtgg tccagcaat gtggtcgat tgcagcaat 
      gcgggctac acaaacgtc cccgagaag ataccgatg 
      gacgccact gaaatatac ctggacggc aataacttg 
      ggtgagctc ggcagtcac gtattcatc ggcaagaaa 
      aaactggaa gtcttgttt ttaaacaac agcaacgtc 
      aacgccgtg cataacagg acattcaac ggtgtcaaa 
      tcgctcaaa gtcctccac atggaaaac aacaatttg 
      gaagaactg cgcggcttc gagttcgac caactggaa 
      aagctcaac gaattgtat ctggaccac aacaagatc 
      gcgttcgtc agcaatcag acgttcaaa aacatgcac 
      gagctggag gttttgaag ctggacgag aataaaatc 
      gtcgacttt atccctcat tgggaaaat gtggctccg 
      cgcgtgact ctcgacggc aacaagtgg tcgtgcgac 
      tgcaattcg ttaggtcgt cttgagatc tggatccgg 
      gagaccgga ggcgaccct tccaaatta ttttgtctg 
      gatcgggac gactccgaa acggtggcc gatgtgatc 
      acccgctgt gataacaaa gacaatgcc attgccacg 
      tcggtcatt caacgagaa gtgctcaaa aacaacccg 
      cttttgggt ggcagttac gtgccgctt ttggccgcc 
      acgctcgtc gctgtcatt gttttctgt ttgctggct 
      gctgtcgtt ttcgtattc cggcaagac gttcgcttg 
      tgggcccac tcgaagtac ggcattcga atttttcaa 
      gacgccgcc actgcgcac gataataaa gacgaacgt 
      gatcgcctc tacgactgc tacatggta tacagccac 
      aaggatgaa gaactggcc gccaggata attgcccca 
      gaacttgaa caactcgga cacacccta tgccttcac 
      aacagagac ttggcgttg cacggcggc gcctcgtat 
      ctcgccgac gccatggtg ggtgcctcg gacgcgtcc 
      cgacgcgtc atcttcctg gtctctcct gccctcatc 
      tgcaacgag tggtctcgt cccgagttc cgcgccgcg 
      ctgcagacg gcccttcgc acggcccac cgtcacaaa 
      ctagtgtgt gttttgagc ggtgaccct ctggagccc 
      atggacccc gagctgcgg gccttgctc cgggcctgc 
      acagtgacg agatggggg gagcgccgc ttctgggag 
      aagctgcgt tatgccatg cccgatgtg gccaacagc 
      aaccggtcg cgccgcggc aagaaacac caccaaccg 
      gaaatccgg tgtaaggtc agtgcgaga tacactgcg 
      gcgccgacg gcccccgac tcctggtac aagtactcg 
      gcgatgccg ggggtgcac gccgcgctg actccaacc 
      cccacgcag agcacttac gtctcgggg gagtcggcc 
      cggagcacc gaggacgaa gggtccagt gcgagcagc 
      cagcattat ggctccgag cagttgaac cacagctac 
      gtgtccatc gacggaagg cctccgccg cagtacaca 
      cagtatccg atatgtagg ccagaaatt gcccaccat 
      ctctattcg accattccg gatacgcaa ccacaatct 
      aggacttac tttgtgtga atgtaagtt gacttaaaa 
      ggctgtttt ttaattaat taggacgta taaagcaat 
      tcgtaggga tttttcata attactcaa atgtcacta 
      ggctctaat tacgatgta atgataact aaattattt 
      gttgtatat attttgtaa atatgcgaa aacgatatt 
      tatttatat tgtacgtgt agtactact tactgaacg 
      cttattagt ca