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Gene information
General information
TC004881 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid6: 1571796..1573390
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004881
    iBeetle screen iB_00781
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004881 RA
      MIEGLSLQV PTLKLKNGE TLPALGLGT WKSKPKDVG 
      QAIKDAIDI GYRLFDCAY IYKNEKEIG EAIREKIKE 
      GVIQRDGIY IVNKLWSTY HRPDLVEPA LDKSLANMG 
      LDYIDLYLI HWPMGLKED GSEYPISKD ARLIFSNVD 
      YVDTWKAME RVCKKGKVK SIGLSNFNR KQIERVIKY 
      AKIHPVVVQ IECHPYLTQ TRMSEFLQS KGIILMAYS 
      PLGSKDRPW AKPGDKELL NDPKMHKVA VKYNKTVAQ 
      ILLRYHIQR GHVVIPKSV NKARLQENF NIFDFELSD 
      EDMKAINTL DCNIRFCPY KEASEHAYY PFKRSDE
    CDS sequence
    • >TC004881 RA
      atgatcgaa ggtctgtcc cttcaagtc ccaaccctc 
      aagctcaag aacggcgag actttgccc gccctcggc 
      ttagggact tggaagtca aaacctaaa gatgtaggc 
      caggccata aaagacgcc atcgatatc ggctaccgc 
      cttttcgat tgtgcttac atttacaaa aacgagaaa 
      gaaattggc gaagcaatt cgggaaaaa atcaaagaa 
      ggggtaatc caacgtgac ggcatttac attgttaac 
      aaattatgg tcaacttat caccgcccg gatttggta 
      gagccggcc cttgacaag tccttggcc aacatgggg 
      cttgactac atcgacttg tacctcatc cactggccc 
      atgggtcta aaggaagat ggcagtgaa tatccgatc 
      agtaaagac gcgcggtta atattcagc aatgttgat 
      tatgtggac acgtggaaa gccatggaa cgggtgtgt 
      aaaaaagga aaggtgaag tcgatcggt ctctccaac 
      tttaacaga aagcaaatt gaacgtgtg atcaaatac 
      gcgaaaatt cacccggtg gtagtccaa atcgagtgt 
      cacccgtac ttgacccaa acccgaatg tcggaattt 
      ttgcaatcg aagggaata attttaatg gcgtatagt 
      ccactcggt tccaaagac aggccgtgg gccaaaccc 
      ggcgataaa gaactactg aacgatccc aaaatgcac 
      aaagttgca gtcaagtac aacaaaact gtagctcag 
      attttgcta agatatcac atccaacgc ggacacgtt 
      gtgatccca aaatcggtc aacaaggcg cgacttcag 
      gaaaatttc aacattttt gatttcgag ttatccgac 
      gaggacatg aaagccatt aacacactg gactgtaac 
      atccggttt tgtccctac aaagaagca tctgaacac 
      gcctattat ccgttcaaa cgaagtgac gagtaa
    mRNA sequence
    • >TC004881 RA
      tctcagtgt caatacaca caatgatcg aaggtctgt 
      cccttcaag tcccaaccc tcaagctca agaacggcg 
      agactttgc ccgccctcg gcttaggga cttggaagt 
      caaaaccta aagatgtag gccaggcca taaaagacg 
      ccatcgata tcggctacc gccttttcg attgtgctt 
      acatttaca aaaacgaga aagaaattg gcgaagcaa 
      ttcgggaaa aaatcaaag aaggggtaa tccaacgtg 
      acggcattt acattgtta acaaattat ggtcaactt 
      atcaccgcc cggatttgg tagagccgg cccttgaca 
      agtccttgg ccaacatgg ggcttgact acatcgact 
      tgtacctca tccactggc ccatgggtc taaaggaag 
      atggcagtg aatatccga tcagtaaag acgcgcggt 
      taatattca gcaatgttg attatgtgg acacgtgga 
      aagccatgg aacgggtgt gtaaaaaag gaaaggtga 
      agtcgatcg gtctctcca actttaaca gaaagcaaa 
      ttgaacgtg tgatcaaat acgcgaaaa ttcacccgg 
      tggtagtcc aaatcgagt gtcacccgt acttgaccc 
      aaacccgaa tgtcggaat ttttgcaat cgaagggaa 
      taattttaa tggcgtata gtccactcg gttccaaag 
      acaggccgt gggccaaac ccggcgata aagaactac 
      tgaacgatc ccaaaatgc acaaagttg cagtcaagt 
      acaacaaaa ctgtagctc agattttgc taagatatc 
      acatccaac gcggacacg ttgtgatcc caaaatcgg 
      tcaacaagg cgcgacttc aggaaaatt tcaacattt 
      ttgatttcg agttatccg acgaggaca tgaaagcca 
      ttaacacac tggactgta acatccggt tttgtccct 
      acaaagaag catctgaac acgcctatt atccgttca 
      aacgaagtg acgagtaaa gtaattttt tattccttt 
      tatatacaa taagttatg atacaaata attaaaaat 
      agcaacgta cgaaatcat tcatcacat tctggtatt 
      gtgggcggt atggaaaac ggcgggggc ggcggaaag 
      ttggggtag gccgggggc gggtagctc gggggttcg 
      ttcttgggc tgctcggca tcttcaaac gcgtcctcg 
      tcccctgat ccattctcc tccaatcgc ttctccaaa 
      ctgatcaaa cgcatttga aaatactgt ttttcctcg 
      ttcagacaa gttaagtcc ttctcctgt tcggcaata 
      acgcaaatc agagattat gttcaaatc ttcttgacg 
      ggacttcct tctctcgta gcgaagtct gacttcctc 
      gaacctgca ataacgggt cacacgctg gacgagatt 
      tatccaaag aa