Status
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Gene information
General information
TC004857 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3236259..3245793
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004857
    iBeetle screen iB_00779

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004857 RA
      MADEQEIRQ LELLCKQLY ESQDTAIRA DAEKALVVF 
      QDGPDALTK CQVLLDRGD SCYAQLLAA TTLTKLVSR 
      SAQGLSLQQ RVDIRNYVL NYLATRPKL PNFVVQALV 
      TLFARITKL GWFDTHKED FVFRNVVND ISKFLQGSV 
      EHCMIGVQL LSQLTCEMN QISEVEANR SLTKHRKIA 
      SSFRDTQLF EIFRLSCTL LGTAIENCK NLNFNDESQ 
      HGLMTQLLR LAQNCLTFD FIGTSTDES SDDLCTVQI 
      PTSWRPAFL DFTTLKLFF DLYHSLPNT LSSLALSCL 
      VQIASVRRS LFSNTERAK FLTHLVNGV KHILQNPQG 
      LSDSANYHE FCRLLARLK SNYQLGELV MVDNYPEAI 
      QLIAKFTVQ SLQMWQFAP NSVHYLLSL WQRMVASVP 
      YVKATEPHL LETYTPEVT NAYITSRLE SVAVVVQEN 
      LEDPLEDLG MVQQQLEQL SVIGRCEYQ KTCTLLVQL 
      FDQAARTYQ ELMAIPRSN APQHVEITI QEGRLTWLV 
      YIIGSAIGG RVSFNSNEE HDTMDGELV CRVLQLMNL 
      TDSRLAQGG CEKLELAML SFFEQFRKI YVGDQVQKN 
      SKVYRRLSE VLGLNDEAT VLSVFIRKI ITNLKYWGR 
      SEQIISKTL QLLNDLSVG YSCVRKLVK LEEVQFMLN 
      NHTSEHFPF LGNTVNVTE MRCRSMFYT SLGRLLMVD 
      LGEDEDRFH TFMLPLTAA FENIGTMLA GADGPMFAP 
      EDAKKALIG LARDLRGLA FSFNTKTSY MMLFDWIYP 
      NYTPILLRA IELWYHDPQ VTTPVLKLF AELVQNRSQ 
      RLQFDVSSP NGILLFREA SKIICSYGS RILNVEVPK 
      EQTYPLKLK GISICFSML KAALCGSYV NFGVFRLYG 
      DETLDNALN IFVKLLLSI PQSDLLDYP KLSQTYYVL 
      LECLAQDHM SFLSTLEPQ VFLYILSSI SEGLTALDT 
      MVCTGCCAT LDHIVTYLF KQLTQKVYP GKKQRVGLA 
      PNSDMFLKV LEMHPEILQ QILSTVLNV IMFEDCRNQ 
      WSMSRPLLG LILLNEEYF NQMRENIIR SQPPDKQAA 
      MVQWFENLM DGIERNLLT KNRDKFTQN LSMFRRDIN 
      DSLKGPNVN TSSASDMMT S
    CDS sequence
    • >TC004857 RA
      atggctgat gaacaggag atccgtcag ctggaactg 
      ctgtgcaag cagctctac gagtctcag gacaccgcg 
      atcagggct gacgcggag aaggccctc gttgtgttc 
      caggacgga cctgatgcc ctcaccaag tgccaggtt 
      ttgttggac agaggtgac tcgtgttat gcacagttg 
      ttggctgct accaccttg accaagttg gtttcgagg 
      agtgcacaa ggattgagc ttgcagcag agggttgac 
      attaggaac tatgttttg aattatctc gccacgagg 
      cccaagttg cccaatttc gttgtccag gctttggtc 
      acgctcttt gcgaggatc accaagttg gggtggttt 
      gacacacac aaggaggac tttgtcttc aggaatgtc 
      gtcaatgat atcagcaag ttcttacag ggttccgtc 
      gagcattgc atgatcggc gtccaactg ctctctcaa 
      ctcacttgc gagatgaac caaatctct gaagttgaa 
      gcaaaccgg tcattaacc aaacaccgc aaaatcgcg 
      tcatcattc cgcgacact caacttttc gaaatattc 
      cgactttcg tgcacactt ctcggcacc gcaatcgag 
      aattgtaaa aatctcaac tttaacgac gaaagtcaa 
      cacggactc atgacccaa ttattacgt ttagcccaa 
      aattgtcta acgtttgat tttatcgga acttcgacc 
      gatgaaagt tcggatgat ctctgcacg gtacaaatc 
      ccgaccagt tggcggccg gcgttttta gattttaca 
      acactaaag ttgtttttt gacttgtat cattccttg 
      cctaatacg ttatcatca ctggcgctt tcttgtctt 
      gtccaaatt gcgtcagtt cgaagatcg ttgtttagc 
      aacaccgag cgggcgaaa ttcttgact catttggtc 
      aacggagtc aagcatatt ctacagaac ccgcaaggg 
      ttgagcgac tcggcgaat tatcacgag ttttgccgg 
      cttttagcc cgactcaag tccaattac cagctcggg 
      gagctggtc atggttgac aattaccca gaagcgatt 
      caattgatc gccaaattt acagttcaa agtttacaa 
      atgtggcaa tttgcgccc aatagcgtc cattatttg 
      ttgagtctt tggcaaaga atggttgct tcggttccg 
      tacgttaaa gcaaccgaa ccacattta ctcgagacg 
      tacactcct gaagtaaca aatgcttat atcacgtcg 
      cgactcgag tcagtcgca gttgtagtc caggaaaat 
      ctagaagat cctctcgaa gatctaggc atggtacaa 
      caacaactt gaacaactg tctgtgatt ggtagatgc 
      gaataccaa aaaacttgc acacttttg gtgcaattg 
      tttgaccaa gcggcaagg acgtaccag gagctgatg 
      gctattccc aggtccaat gcgccacaa cacgtcgaa 
      attacgata caagaaggt aggttaact tggttggtg 
      tacatcatc ggatcggcg ataggtggt cgtgtctcg 
      ttcaatagc aacgaagag cacgataca atggatggg 
      gagttggtg tgtcgtgtg ttgcaattg atgaatttg 
      accgattcg agattagcg caaggcggt tgtgaaaaa 
      ctcgagttg gcaatgttg agtttcttc gaacagttt 
      agaaagatc tacgttggg gatcaggtg caaaaaaat 
      tcgaaagtt taccgaaga ctatcagag gtgttagga 
      ttgaatgac gaggcgacc gttttgagt gtttttatt 
      aggaaaata atcacgaat ttgaagtat tggggccgc 
      agcgagcaa atcattagc aaaactttg caattgctt 
      aacgattta tcggttggt tatagttgc gttcgcaaa 
      ctagtcaaa cttgaagaa gtccaattc atgttgaat 
      aatcataca agtgaacat ttcccgttt ttgggtaac 
      acggttaac gtaacggag atgcgatgt aggtctatg 
      ttttatacg tctttgggg cgactttta atggtggat 
      ttgggcgag gatgaggat cggtttcat acttttatg 
      ttacctttg actgccgcg tttgagaat attggtaca 
      atgttggcg ggtgctgat ggtccaatg tttgcgcct 
      gaagatgcg aaaaaggcg ctgattggt ctagcgagg 
      gatttgaga ggtttggcg ttctctttc aacacgaag 
      acgtcgtat atgatgctt tttgattgg atatatccg 
      aattatacc ccgatttta ttacgagcg atagaattg 
      tggtatcac gacccgcag gtgacaact cccgtcttg 
      aaacttttt gccgaattg gtacaaaat cgctcgcaa 
      aggttacaa ttcgatgtt tcgtcacca aacggaatt 
      ttattattt agagaagcg agtaaaata atttgtagt 
      tacggtagt agaatatta aatgtagaa gtaccaaaa 
      gaacaaacg tatccgttg aaattgaag gggatatca 
      atatgtttt tccatgctc aaggcggct ctgtgcggc 
      agttacgta aactttgga gtgtttcgc ttatacggc 
      gacgagacg ttagataat gctcttaat atatttgta 
      aaattatta ctatcgata cctcaatcc gatttattg 
      gattatcca aagttgtcg caaacttat tacgtattg 
      ttggaatgc ctggcacag gaccacatg agtttttta 
      tcaactttg gagccgcaa gtatttttg tacatcctc 
      tcctcgatt tccgagggt ctaactgcc ttagataca 
      atggtttgt accggctgt tgtgcaacg ctggatcac 
      attgtgacc tatttattc aaacagttg acgcaaaaa 
      gtatatccg ggaaaaaaa caaagagtc ggtctggca 
      ccgaacagc gacatgttc ctaaaagtg ttagagatg 
      catccggag attttgcaa caaatttta agtactgtt 
      ttaaatgtg atcatgttt gaagattgt cggaatcag 
      tggtcgatg agtcggcca ttgctaggt ctcattctt 
      ttaaacgag gagtatttc aatcaaatg agagagaat 
      atcatacgg agtcagccg ccggataaa caagcggct 
      atggtgcag tggttcgaa aatttaatg gacgggata 
      gaaaggaat ttgcttacg aaaaatagg gacaaattt 
      actcaaaat ttatcaatg tttcggcga gatattaat 
      gactcttta aaagggccc aatgtaaat acttcgtct 
      gctagtgat atgatgaca tcatag
    mRNA sequence
    • >TC004857 RA
      ccgttccgg taaatttgc gttatgttg tttttgagt 
      gttcacagt gaaaagttg ataaaagtg gtgcgaatc 
      aaccttttt gaacacttg cactatcaa ccaagctct 
      cagccaaat caatccaat gaaagtgag cgataatgg 
      ctgatgaac aggagatcc gtcagctgg aactgctgt 
      gcaagcagc tctacgagt ctcaggaca ccgcgatca 
      gggctgacg cggagaagg ccctcgttg tgttccagg 
      acggacctg atgccctca ccaagtgcc aggttttgt 
      tggacagag gtgactcgt gttatgcac agttgttgg 
      ctgctacca ccttgacca agttggttt cgaggagtg 
      cacaaggat tgagcttgc agcagaggg ttgacatta 
      ggaactatg ttttgaatt atctcgcca cgaggccca 
      agttgccca atttcgttg tccaggctt tggtcacgc 
      tctttgcga ggatcacca agttggggt ggtttgaca 
      cacacaagg aggactttg tcttcagga atgtcgtca 
      atgatatca gcaagttct tacagggtt ccgtcgagc 
      attgcatga tcggcgtcc aactgctct ctcaactca 
      cttgcgaga tgaaccaaa tctctgaag ttgaagcaa 
      accggtcat taaccaaac accgcaaaa tcgcgtcat 
      cattccgcg acactcaac ttttcgaaa tattccgac 
      tttcgtgca cacttctcg gcaccgcaa tcgagaatt 
      gtaaaaatc tcaacttta acgacgaaa gtcaacacg 
      gactcatga cccaattat tacgtttag cccaaaatt 
      gtctaacgt ttgatttta tcggaactt cgaccgatg 
      aaagttcgg atgatctct gcacggtac aaatcccga 
      ccagttggc ggccggcgt ttttagatt ttacaacac 
      taaagttgt tttttgact tgtatcatt ccttgccta 
      atacgttat catcactgg cgctttctt gtcttgtcc 
      aaattgcgt cagttcgaa gatcgttgt ttagcaaca 
      ccgagcggg cgaaattct tgactcatt tggtcaacg 
      gagtcaagc atattctac agaacccgc aagggttga 
      gcgactcgg cgaattatc acgagtttt gccggcttt 
      tagcccgac tcaagtcca attaccagc tcggggagc 
      tggtcatgg ttgacaatt acccagaag cgattcaat 
      tgatcgcca aatttacag ttcaaagtt tacaaatgt 
      ggcaatttg cgcccaata gcgtccatt atttgttga 
      gtctttggc aaagaatgg ttgcttcgg ttccgtacg 
      ttaaagcaa ccgaaccac atttactcg agacgtaca 
      ctcctgaag taacaaatg cttatatca cgtcgcgac 
      tcgagtcag tcgcagttg tagtccagg aaaatctag 
      aagatcctc tcgaagatc taggcatgg tacaacaac 
      aacttgaac aactgtctg tgattggta gatgcgaat 
      accaaaaaa cttgcacac ttttggtgc aattgtttg 
      accaagcgg caaggacgt accaggagc tgatggcta 
      ttcccaggt ccaatgcgc cacaacacg tcgaaatta 
      cgatacaag aaggtaggt taacttggt tggtgtaca 
      tcatcggat cggcgatag gtggtcgtg tctcgttca 
      atagcaacg aagagcacg atacaatgg atggggagt 
      tggtgtgtc gtgtgttgc aattgatga atttgaccg 
      attcgagat tagcgcaag gcggttgtg aaaaactcg 
      agttggcaa tgttgagtt tcttcgaac agtttagaa 
      agatctacg ttggggatc aggtgcaaa aaaattcga 
      aagtttacc gaagactat cagaggtgt taggattga 
      atgacgagg cgaccgttt tgagtgttt ttattagga 
      aaataatca cgaatttga agtattggg gccgcagcg 
      agcaaatca ttagcaaaa ctttgcaat tgcttaacg 
      atttatcgg ttggttata gttgcgttc gcaaactag 
      tcaaacttg aagaagtcc aattcatgt tgaataatc 
      atacaagtg aacatttcc cgtttttgg gtaacacgg 
      ttaacgtaa cggagatgc gatgtaggt ctatgtttt 
      atacgtctt tggggcgac ttttaatgg tggatttgg 
      gcgaggatg aggatcggt ttcatactt ttatgttac 
      ctttgactg ccgcgtttg agaatattg gtacaatgt 
      tggcgggtg ctgatggtc caatgtttg cgcctgaag 
      atgcgaaaa aggcgctga ttggtctag cgagggatt 
      tgagaggtt tggcgttct ctttcaaca cgaagacgt 
      cgtatatga tgctttttg attggatat atccgaatt 
      ataccccga ttttattac gagcgatag aattgtggt 
      atcacgacc cgcaggtga caactcccg tcttgaaac 
      tttttgccg aattggtac aaaatcgct cgcaaaggt 
      tacaattcg atgtttcgt caccaaacg gaattttat 
      tatttagag aagcgagta aaataattt gtagttacg 
      gtagtagaa tattaaatg tagaagtac caaaagaac 
      aaacgtatc cgttgaaat tgaagggga tatcaatat 
      gtttttcca tgctcaagg cggctctgt gcggcagtt 
      acgtaaact ttggagtgt ttcgcttat acggcgacg 
      agacgttag ataatgctc ttaatatat ttgtaaaat 
      tattactat cgatacctc aatccgatt tattggatt 
      atccaaagt tgtcgcaaa cttattacg tattgttgg 
      aatgcctgg cacaggacc acatgagtt ttttatcaa 
      ctttggagc cgcaagtat ttttgtaca tcctctcct 
      cgatttccg agggtctaa ctgccttag atacaatgg 
      tttgtaccg gctgttgtg caacgctgg atcacattg 
      tgacctatt tattcaaac agttgacgc aaaaagtat 
      atccgggaa aaaaacaaa gagtcggtc tggcaccga 
      acagcgaca tgttcctaa aagtgttag agatgcatc 
      cggagattt tgcaacaaa ttttaagta ctgttttaa 
      atgtgatca tgtttgaag attgtcgga atcagtggt 
      cgatgagtc ggccattgc taggtctca ttcttttaa 
      acgaggagt atttcaatc aaatgagag agaatatca 
      tacggagtc agccgccgg ataaacaag cggctatgg 
      tgcagtggt tcgaaaatt taatggacg ggatagaaa 
      ggaatttgc ttacgaaaa atagggaca aatttactc 
      aaaatttat caatgtttc ggcgagata ttaatgact 
      ctttaaaag ggcccaatg taaatactt cgtctgcta 
      gtgatatga tgacatcat agtgaatgt gtgctgaaa 
      aatactaat tggttgtta ctttaggta cacattgca 
      tttattttt gtcgggatc gcaaggaaa aaatgcagt 
      gctg
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