Status
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Gene information
General information
TC004841 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2733018..2734443
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004841
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00776

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004841 RA
      MENSPLNKA HQLDRRAEA LLKQKKFDE SIECHQNAM 
      KYLHDALNL TQNPRSLES IQLQIDHHQ KQVDSLKIK 
      KLQYEDLRR YQESVKNRV VNFDSICEN GETLQHEIF 
      RTIETHDSL IEVMMKRNC GEECLRDFS SDDEKVSSD 
      TMKVSGKKR EVDDSTLLE EFKGLSEKL REFVQILVL 
      QLEEKDNEI ARLKAEIYK LECEKNKDG TKNSNTLRV 
      ITDSSGGTS PYVFSPCSE LSPEVHDIR GLPPLAPLE 
      MPNFDFSSL VKNANNLNL NGTNN
    CDS sequence
    • >TC004841 RA
      atggaaaat tctccgtta aataaggct caccagctg 
      gatcggcgc gctgaagcc ttattgaaa cagaaaaaa 
      ttcgacgaa agcattgaa tgccaccaa aacgctatg 
      aaatatctc cacgatgcg ttaaatttg actcaaaac 
      ccgcgctcg ttagaatca atccaattg caaattgac 
      caccaccaa aaacaagtc gactcgtta aaaataaaa 
      aaattgcag tacgaagat ttgagacgc taccaagag 
      tcggtgaaa aaccgtgtt gttaacttc gattcgatt 
      tgtgagaac ggggaaacg ctccaacac gaaatattc 
      cgcaccata gaaacgcac gattcttta atcgaggtt 
      atgatgaag cggaattgt ggggaagag tgtcttagg 
      gacttttca agtgacgac gaaaaagtc tccagcgac 
      acgatgaaa gtctcgggc aagaagcga gaggtggac 
      gattcaaca ctccttgaa gagttcaag gggctaagc 
      gagaaatta cgggagttt gtacaaatt ttggttctc 
      caactggag gagaaagac aatgaaatt gcacgactc 
      aaagccgag atttacaaa ttagagtgt gaaaaaaat 
      aaagatggt actaaaaat agcaacact ctcagagtc 
      attaccgac tcttcggga gggacttcc ccttatgtg 
      ttctcaccc tgtagtgaa ctaagtccc gaagtacat 
      gacatcaga gggcttcca cctttggca cccttagaa 
      atgccaaat tttgatttc tcaagtttg gtcaaaaac 
      gctaacaac ttgaacctc aacggtaca aacaactag
    mRNA sequence
    • >TC004841 RA
      cagccgatt gacagttat ttttaactg gaacattta 
      ccttttatt tacattttt gtgatacgt ttagttgtt 
      agtgacttg aattgagcc caatggaaa attctccgt 
      taaataagg ctcaccagc tggatcggc gcgctgaag 
      ccttattga aacagaaaa aattcgacg aaagcattg 
      aatgccacc aaaacgcta tgaaatatc tccacgatg 
      cgttaaatt tgactcaaa acccgcgct cgttagaat 
      caatccaat tgcaaattg accaccacc aaaaacaag 
      tcgactcgt taaaaataa aaaaattgc agtacgaag 
      atttgagac gctaccaag agtcggtga aaaaccgtg 
      ttgttaact tcgattcga tttgtgaga acggggaaa 
      cgctccaac acgaaatat tccgcacca tagaaacgc 
      acgattctt taatcgagg ttatgatga agcggaatt 
      gtggggaag agtgtctta gggactttt caagtgacg 
      acgaaaaag tctccagcg acacgatga aagtctcgg 
      gcaagaagc gagaggtgg acgattcaa cactccttg 
      aagagttca aggggctaa gcgagaaat tacgggagt 
      ttgtacaaa ttttggttc tccaactgg aggagaaag 
      acaatgaaa ttgcacgac tcaaagccg agatttaca 
      aattagagt gtgaaaaaa ataaagatg gtactaaaa 
      atagcaaca ctctcagag tcattaccg actcttcgg 
      gagggactt ccccttatg tgttctcac cctgtagtg 
      aactaagtc ccgaagtac atgacatca gagggcttc 
      cacctttgg cacccttag aaatgccaa attttgatt 
      tctcaagtt tggtcaaaa acgctaaca acttgaacc 
      tcaacggta caaacaact aggaattca agtattacg 
      caagatttg tgttcaatt cacgaccag ttatttatg 
      ttttttatt gttcatcag acgtactaa gattaaaaa 
      tactaacaa agtttctga aaaatatat tgatcgtta 
      catcaatca agcattaaa tcatatcaa cactaacta 
      agataatac agtagaacc tctgtcgcc tcaaagcat 
      attcagcga tacagattt cagaagtaa ttaactaat 
      gtttagttt ttattttaa ttaaaaaat gattcagga 
      gacatgttt tatagaaat ttgttggtt atgttcgat 
      cgaaaatgg tgatttttg tgtgcttat acgtataac 
      cttacggaa tgatagctt ttgacaaat taataataa 
      cgaataata gtgaaaaaa gtta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY