| General information | |||
| TC004831 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 2540684..2543120 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004831 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00774 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 11.11% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
- >TC004831 RA
MTILEDNVI PLRSKLRLP TQNQAPKRE GSFLEAPYR KRQELDMLN GYRAPEAGD FPVATLANM GNTCFLNSV LYTLRYAPT FLHNLHHLI GDLALVSSR LSQTKAKTS SLGRNVGAI TGPSSRSTS SKDLLALSS SDIIPKSKV QIVTEKLHE LYVTMHNLE VKESSDAYQ PVALLQAIR EANSIFEGN HQQDAHELL VYLLDNIRE TCDLLTQQV QQNPDLLTE SEIIPSPNS SKIWSVRRS WKITKKKEK NAKEAISEE QVNGASVTD TEDACSVDG GGDNNKKKL GYNFVAEDF EGVTLRRTR CLECESVTE RKEPFYDIP VPIPVKDTD FEPDSASEI YRKACVTSE KLCDANKYL CEKCQRYNE ASRDVLFEK LPNIMVLQL KRFTTSSAG VQKVNTYLP TPLELECFC ESCCNVEED ETPPHRYRL CCVIMHLGA SMASGHYIA YVKASDHFE DYVECSRNM PKGSLSASS SEKSLNILK FFKPRTIGS LSENKSILS KSVVNGVRM CKSMDCCGV KINKNVVEN VVNSYPRNK EDFCNGGPE DMWLECDDE SVKPITSQE FKEELAFKP NSTSTPYLL FYSKITDIP LD
- >TC004831 RA
atgacaatt ctggaagat aacgtaatc cccttgcgt agcaaacta cgtctcccg acgcaaaat caggccccg aagcgcgaa ggctcattc ctcgaagcc ccctatcgc aagcgccag gagctggac atgttgaac ggctatcgg gcccccgaa gcgggcgat ttcccagtc gccaccctc gcgaacatg ggcaacacg tgcttcctc aacagcgtt ctgtacact ctgcgctac gccccgacg ttcctacat aacctccac cacctgatc ggcgacctg gcgctcgta agctcgcgc ctcagccag accaaagcc aagacctca tcgctgggg cgcaacgtg ggggccatc acgggcccc agctcgcgc agcaccagc agcaaggac ttgctcgcc ttgagctcc tccgacatt atccccaag tctaaggtg caaatcgtc accgagaag ctgcacgag ctctacgtc acgatgcac aatttggag gtgaaggag tcgagcgat gcctaccaa cctgttgca ctcctccaa gccattcgg gaggcgaat tcgattttc gaagggaac caccagcaa gacgcccat gagttgctc gtctacttg ttggataat ataagggag acatgtgac ttgcttacg caacaagtg caacaaaac cccgatttg ttgactgag agtgagatc attccctcg cccaacagc agcaagatt tggagtgtg cgacgttcg tggaaaatc acgaagaag aaggaaaag aacgcgaag gaggctata agtgaggag caagtcaac ggtgcgagt gtcactgac acggaggac gcctgttcc gtcgacgga ggcggcgat aacaacaag aagaagctg gggtacaac ttcgtcgca gaggacttc gaaggtgtg accctccgg cgcaccagg tgtctcgaa tgcgagagc gtgaccgag cgcaaggag ccgttctac gacatcccg gtgccaatc cccgtcaaa gacaccgat ttcgagccg gactctgcg agcgaaatc taccgcaaa gcgtgtgtc actagtgaa aagttgtgc gatgccaat aaatatttg tgtgagaag tgccagcgt tataatgag gctagtcgt gacgtcctc ttcgagaaa ctcccgaat attatggtc ctgcaactc aagcgcttc accacgtct tccgccggc gtgcagaaa gtcaacact tacctcccg acgccgctc gaactggag tgcttttgc gagtcgtgt tgcaacgtg gaggaggac gagacgccc ccgcatcgc taccgcctg tgctgcgtt atcatgcac ctgggggcg tccatggcc tcgggccac tacatcgcc tacgtgaag gcgtccgat cacttcgag gactacgtc gagtgctcc aggaatatg cccaagggg agtttgtcg gcgagcagc agcgagaag tcgctcaac attttgaag ttttttaag cccaggacg atagggtcg ttaagtgag aacaagagt attttgtcg aaaagtgtg gtgaacggt gtgaggatg tgcaaaagt atggactgt tgtggcgtt aaaattaat aaaaatgtg gtggagaac gttgttaac tcttatccg aggaacaaa gaggatttt tgcaacggc gggcccgag gatatgtgg ttggagtgc gatgatgaa agtgttaaa cccataacg agtcaagag tttaaggag gagttggcg ttcaaaccc aactccacc tcgacgccc tacttgctg ttctattcc aaaattact gatatacct ttagattaa
- >TC004831 RA
ggaaatgtt aagcaagtc tccggtgtg gagtgtgtc actaatgat tgctaatta ccgcaatta ataggcttt ttccactgt aatgaattc aaaagccca acatttatc caacgattg agccaccgt cacacatgt ttggacaag aagatgaca attctggaa gataacgta atccccttg cgtagcaaa ctacgtctc ccgacgcaa aatcaggcc ccgaagcgc gaaggctca ttcctcgaa gccccctat cgcaagcgc caggagctg gacatgttg aacggctat cgggccccc gaagcgggc gatttccca gtcgccacc ctcgcgaac atgggcaac acgtgcttc ctcaacagc gttctgtac actctgcgc tacgccccg acgttccta cataacctc caccacctg atcggcgac ctggcgctc gtaagctcg cgcctcagc cagaccaaa gccaagacc tcatcgctg gggcgcaac gtgggggcc atcacgggc cccagctcg cgcagcacc agcagcaag gacttgctc gccttgagc tcctccgac attatcccc aagtctaag gtgcaaatc gtcaccgag aagctgcac gagctctac gtcacgatg cacaatttg gaggtgaag gagtcgagc gatgcctac caacctgtt gcactcctc caagccatt cgggaggcg aattcgatt ttcgaaggg aaccaccag caagacgcc catgagttg ctcgtctac ttgttggat aatataagg gagacatgt gacttgctt acgcaacaa gtgcaacaa aaccccgat ttgttgact gagagtgag atcattccc tcgcccaac agcagcaag atttggagt gtgcgacgt tcgtggaaa atcacgaag aagaaggaa aagaacgcg aaggaggct ataagtgag gagcaagtc aacggtgcg agtgtcact gacacggag gacgcctgt tccgtcgac ggaggcggc gataacaac aagaagaag ctggggtac aacttcgtc gcagaggac ttcgaaggt gtgaccctc cggcgcacc aggtgtctc gaatgcgag agcgtgacc gagcgcaag gagccgttc tacgacatc ccggtgcca atccccgtc aaagacacc gatttcgag ccggactct gcgagcgaa atctaccgc aaagcgtgt gtcactagt gaaaagttg tgcgatgcc aataaatat ttgtgtgag aagtgccag cgttataat gaggctagt cgtgacgtc ctcttcgag aaactcccg aatattatg gtcctgcaa ctcaagcgc ttcaccacg tcttccgcc ggcgtgcag aaagtcaac acttacctc ccgacgccg ctcgaactg gagtgcttt tgcgagtcg tgttgcaac gtggaggag gacgagacg cccccgcat cgctaccgc ctgtgctgc gttatcatg cacctgggg gcgtccatg gcctcgggc cactacatc gcctacgtg aaggcgtcc gatcacttc gaggactac gtcgagtgc tccaggaat atgcccaag gggagtttg tcggcgagc agcagcgag aagtcgctc aacattttg aagtttttt aagcccagg acgataggg tcgttaagt gagaacaag agtattttg tcgaaaagt gtggtgaac ggtgtgagg atgtgcaaa agtatggac tgttgtggc gttaaaatt aataaaaat gtggtggag aacgttgtt aactcttat ccgaggaac aaagaggat ttttgcaac ggcgggccc gaggatatg tggttggag tgcgatgat gaaagtgtt aaacccata acgagtcaa gagtttaag gaggagttg gcgttcaaa cccaactcc acctcgacg ccctacttg ctgttctat tccaaaatt actgatata cctttagat taatttttg cgttgaagc acttaggta agatcaatt atttaggaa gtacaaaac ccctcgact aactgaatg tgatattca aagaatctt tatctataa attctgtta tgcacgtcc aagtctttg ggcccgtgc aaaaataat tgtttcctt gatattgcg caaaatttg taatgtatg gctgtgaga cacatttct ttaagttgt tcgcatgtt tttagtaat tatttttct aaaagttga taggttcta aacatttag atcgaacag ttcgaatgg tagttgagg ataattcca ttaaaaaaa ggtccgcaa tggaccgct ttatacgag taggtccta aataccatt acgaacctt ttcctgcgt caaggaatt ctgacctca tcacaaaac ttgtcatga ttattacaa ctttgcgat tgtaatatt aaacgttaa tcactcca
