Status
Please wait ...
Gene information
General information
TC004831 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2540684..2543120
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004831
    iBeetle screen iB_00774

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 11.11% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004831 RA
      MTILEDNVI PLRSKLRLP TQNQAPKRE GSFLEAPYR 
      KRQELDMLN GYRAPEAGD FPVATLANM GNTCFLNSV 
      LYTLRYAPT FLHNLHHLI GDLALVSSR LSQTKAKTS 
      SLGRNVGAI TGPSSRSTS SKDLLALSS SDIIPKSKV 
      QIVTEKLHE LYVTMHNLE VKESSDAYQ PVALLQAIR 
      EANSIFEGN HQQDAHELL VYLLDNIRE TCDLLTQQV 
      QQNPDLLTE SEIIPSPNS SKIWSVRRS WKITKKKEK 
      NAKEAISEE QVNGASVTD TEDACSVDG GGDNNKKKL 
      GYNFVAEDF EGVTLRRTR CLECESVTE RKEPFYDIP 
      VPIPVKDTD FEPDSASEI YRKACVTSE KLCDANKYL 
      CEKCQRYNE ASRDVLFEK LPNIMVLQL KRFTTSSAG 
      VQKVNTYLP TPLELECFC ESCCNVEED ETPPHRYRL 
      CCVIMHLGA SMASGHYIA YVKASDHFE DYVECSRNM 
      PKGSLSASS SEKSLNILK FFKPRTIGS LSENKSILS 
      KSVVNGVRM CKSMDCCGV KINKNVVEN VVNSYPRNK 
      EDFCNGGPE DMWLECDDE SVKPITSQE FKEELAFKP 
      NSTSTPYLL FYSKITDIP LD
    CDS sequence
    • >TC004831 RA
      atgacaatt ctggaagat aacgtaatc cccttgcgt 
      agcaaacta cgtctcccg acgcaaaat caggccccg 
      aagcgcgaa ggctcattc ctcgaagcc ccctatcgc 
      aagcgccag gagctggac atgttgaac ggctatcgg 
      gcccccgaa gcgggcgat ttcccagtc gccaccctc 
      gcgaacatg ggcaacacg tgcttcctc aacagcgtt 
      ctgtacact ctgcgctac gccccgacg ttcctacat 
      aacctccac cacctgatc ggcgacctg gcgctcgta 
      agctcgcgc ctcagccag accaaagcc aagacctca 
      tcgctgggg cgcaacgtg ggggccatc acgggcccc 
      agctcgcgc agcaccagc agcaaggac ttgctcgcc 
      ttgagctcc tccgacatt atccccaag tctaaggtg 
      caaatcgtc accgagaag ctgcacgag ctctacgtc 
      acgatgcac aatttggag gtgaaggag tcgagcgat 
      gcctaccaa cctgttgca ctcctccaa gccattcgg 
      gaggcgaat tcgattttc gaagggaac caccagcaa 
      gacgcccat gagttgctc gtctacttg ttggataat 
      ataagggag acatgtgac ttgcttacg caacaagtg 
      caacaaaac cccgatttg ttgactgag agtgagatc 
      attccctcg cccaacagc agcaagatt tggagtgtg 
      cgacgttcg tggaaaatc acgaagaag aaggaaaag 
      aacgcgaag gaggctata agtgaggag caagtcaac 
      ggtgcgagt gtcactgac acggaggac gcctgttcc 
      gtcgacgga ggcggcgat aacaacaag aagaagctg 
      gggtacaac ttcgtcgca gaggacttc gaaggtgtg 
      accctccgg cgcaccagg tgtctcgaa tgcgagagc 
      gtgaccgag cgcaaggag ccgttctac gacatcccg 
      gtgccaatc cccgtcaaa gacaccgat ttcgagccg 
      gactctgcg agcgaaatc taccgcaaa gcgtgtgtc 
      actagtgaa aagttgtgc gatgccaat aaatatttg 
      tgtgagaag tgccagcgt tataatgag gctagtcgt 
      gacgtcctc ttcgagaaa ctcccgaat attatggtc 
      ctgcaactc aagcgcttc accacgtct tccgccggc 
      gtgcagaaa gtcaacact tacctcccg acgccgctc 
      gaactggag tgcttttgc gagtcgtgt tgcaacgtg 
      gaggaggac gagacgccc ccgcatcgc taccgcctg 
      tgctgcgtt atcatgcac ctgggggcg tccatggcc 
      tcgggccac tacatcgcc tacgtgaag gcgtccgat 
      cacttcgag gactacgtc gagtgctcc aggaatatg 
      cccaagggg agtttgtcg gcgagcagc agcgagaag 
      tcgctcaac attttgaag ttttttaag cccaggacg 
      atagggtcg ttaagtgag aacaagagt attttgtcg 
      aaaagtgtg gtgaacggt gtgaggatg tgcaaaagt 
      atggactgt tgtggcgtt aaaattaat aaaaatgtg 
      gtggagaac gttgttaac tcttatccg aggaacaaa 
      gaggatttt tgcaacggc gggcccgag gatatgtgg 
      ttggagtgc gatgatgaa agtgttaaa cccataacg 
      agtcaagag tttaaggag gagttggcg ttcaaaccc 
      aactccacc tcgacgccc tacttgctg ttctattcc 
      aaaattact gatatacct ttagattaa
    mRNA sequence
    • >TC004831 RA
      ggaaatgtt aagcaagtc tccggtgtg gagtgtgtc 
      actaatgat tgctaatta ccgcaatta ataggcttt 
      ttccactgt aatgaattc aaaagccca acatttatc 
      caacgattg agccaccgt cacacatgt ttggacaag 
      aagatgaca attctggaa gataacgta atccccttg 
      cgtagcaaa ctacgtctc ccgacgcaa aatcaggcc 
      ccgaagcgc gaaggctca ttcctcgaa gccccctat 
      cgcaagcgc caggagctg gacatgttg aacggctat 
      cgggccccc gaagcgggc gatttccca gtcgccacc 
      ctcgcgaac atgggcaac acgtgcttc ctcaacagc 
      gttctgtac actctgcgc tacgccccg acgttccta 
      cataacctc caccacctg atcggcgac ctggcgctc 
      gtaagctcg cgcctcagc cagaccaaa gccaagacc 
      tcatcgctg gggcgcaac gtgggggcc atcacgggc 
      cccagctcg cgcagcacc agcagcaag gacttgctc 
      gccttgagc tcctccgac attatcccc aagtctaag 
      gtgcaaatc gtcaccgag aagctgcac gagctctac 
      gtcacgatg cacaatttg gaggtgaag gagtcgagc 
      gatgcctac caacctgtt gcactcctc caagccatt 
      cgggaggcg aattcgatt ttcgaaggg aaccaccag 
      caagacgcc catgagttg ctcgtctac ttgttggat 
      aatataagg gagacatgt gacttgctt acgcaacaa 
      gtgcaacaa aaccccgat ttgttgact gagagtgag 
      atcattccc tcgcccaac agcagcaag atttggagt 
      gtgcgacgt tcgtggaaa atcacgaag aagaaggaa 
      aagaacgcg aaggaggct ataagtgag gagcaagtc 
      aacggtgcg agtgtcact gacacggag gacgcctgt 
      tccgtcgac ggaggcggc gataacaac aagaagaag 
      ctggggtac aacttcgtc gcagaggac ttcgaaggt 
      gtgaccctc cggcgcacc aggtgtctc gaatgcgag 
      agcgtgacc gagcgcaag gagccgttc tacgacatc 
      ccggtgcca atccccgtc aaagacacc gatttcgag 
      ccggactct gcgagcgaa atctaccgc aaagcgtgt 
      gtcactagt gaaaagttg tgcgatgcc aataaatat 
      ttgtgtgag aagtgccag cgttataat gaggctagt 
      cgtgacgtc ctcttcgag aaactcccg aatattatg 
      gtcctgcaa ctcaagcgc ttcaccacg tcttccgcc 
      ggcgtgcag aaagtcaac acttacctc ccgacgccg 
      ctcgaactg gagtgcttt tgcgagtcg tgttgcaac 
      gtggaggag gacgagacg cccccgcat cgctaccgc 
      ctgtgctgc gttatcatg cacctgggg gcgtccatg 
      gcctcgggc cactacatc gcctacgtg aaggcgtcc 
      gatcacttc gaggactac gtcgagtgc tccaggaat 
      atgcccaag gggagtttg tcggcgagc agcagcgag 
      aagtcgctc aacattttg aagtttttt aagcccagg 
      acgataggg tcgttaagt gagaacaag agtattttg 
      tcgaaaagt gtggtgaac ggtgtgagg atgtgcaaa 
      agtatggac tgttgtggc gttaaaatt aataaaaat 
      gtggtggag aacgttgtt aactcttat ccgaggaac 
      aaagaggat ttttgcaac ggcgggccc gaggatatg 
      tggttggag tgcgatgat gaaagtgtt aaacccata 
      acgagtcaa gagtttaag gaggagttg gcgttcaaa 
      cccaactcc acctcgacg ccctacttg ctgttctat 
      tccaaaatt actgatata cctttagat taatttttg 
      cgttgaagc acttaggta agatcaatt atttaggaa 
      gtacaaaac ccctcgact aactgaatg tgatattca 
      aagaatctt tatctataa attctgtta tgcacgtcc 
      aagtctttg ggcccgtgc aaaaataat tgtttcctt 
      gatattgcg caaaatttg taatgtatg gctgtgaga 
      cacatttct ttaagttgt tcgcatgtt tttagtaat 
      tatttttct aaaagttga taggttcta aacatttag 
      atcgaacag ttcgaatgg tagttgagg ataattcca 
      ttaaaaaaa ggtccgcaa tggaccgct ttatacgag 
      taggtccta aataccatt acgaacctt ttcctgcgt 
      caaggaatt ctgacctca tcacaaaac ttgtcatga 
      ttattacaa ctttgcgat tgtaatatt aaacgttaa 
      tcactcca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY